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Protein

Diacylglycerol kinase theta

Gene

DGKQ

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor (By similarity). May be involved in cAMP-induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity.By similarity1 Publication

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Activity regulationi

Inactivated by binding to RHOA. Not inhibited by phosphatidylserine.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri60 – 108Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST49
Zinc fingeri121 – 168Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST48
Zinc fingeri183 – 234Phorbol-ester/DAG-type 3PROSITE-ProRule annotationAdd BLAST52

GO - Molecular functioni

  • activating transcription factor binding Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • diacylglycerol kinase activity Source: UniProtKB
  • kinase binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • NAD+ kinase activity Source: InterPro
  • phospholipase binding Source: UniProtKB
  • transmembrane receptor protein tyrosine kinase activator activity Source: ParkinsonsUK-UCL

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-114508 Effects of PIP2 hydrolysis

Chemistry databases

SwissLipidsiSLP:000000740

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase theta (EC:2.7.1.107)
Short name:
DAG kinase theta
Alternative name(s):
Diglyceride kinase theta
Short name:
DGK-theta
Gene namesi
Name:DGKQ
Synonyms:DAGK4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000145214.13
HGNCiHGNC:2856 DGKQ
MIMi601207 gene
neXtProtiNX_P52824

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi100C → G: Abolishes translocation to the plasma membrane. 1 Publication1
Mutagenesisi160C → G: Abolishes translocation to the plasma membrane. 1 Publication1
Mutagenesisi226C → G: Abolishes translocation to the plasma membrane. 1 Publication1
Mutagenesisi237G → R: Abolishes enzymatic activity; no effect on translocation to the plasma membrane. 2 Publications1
Mutagenesisi245P → A or L: Greatly reduces enzymatic activity. 1 Publication1
Mutagenesisi246P → L: Abolishes enzymatic activity. 1 Publication1

Organism-specific databases

DisGeNETi1609
OpenTargetsiENSG00000145214
PharmGKBiPA27317

Polymorphism and mutation databases

BioMutaiDGKQ
DMDMi257051005

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002184671 – 942Diacylglycerol kinase thetaAdd BLAST942

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei22PhosphoserineBy similarity1
Modified residuei26PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by PRKCE and PRKCH in vitro.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP52824
MaxQBiP52824
PaxDbiP52824
PeptideAtlasiP52824
PRIDEiP52824
ProteomicsDBi56542

PTM databases

iPTMnetiP52824
PhosphoSitePlusiP52824

Expressioni

Gene expression databases

BgeeiENSG00000145214 Expressed in 177 organ(s), highest expression level in right hemisphere of cerebellum
CleanExiHS_DGKQ
ExpressionAtlasiP52824 baseline and differential
GenevisibleiP52824 HS

Organism-specific databases

HPAiCAB033835
HPA026797

Interactioni

Subunit structurei

Interacts with RHOA (constitutively activated, GTP-bound); the interaction inhibits DGKQ. Interacts with PRKCE. Interacts with PRKCH. Interacts with PLCB1. Interacts with NR5A1; the interaction requires the LXXLL motif 1 and LXXLL motif 2 in DGKQ.5 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi107979, 10 interactors
IntActiP52824, 1 interactor
STRINGi9606.ENSP00000273814

Structurei

3D structure databases

ProteinModelPortaliP52824
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini395 – 494Ras-associatingPROSITE-ProRule annotationAdd BLAST100
Domaini584 – 721DAGKcPROSITE-ProRule annotationAdd BLAST138

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi555 – 559LXXLL motif 15
Motifi574 – 578LXXLL motif 25

Domaini

The L-X-X-L-L repeats are implicated in binding to the nuclear receptor NR5A1.

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri60 – 108Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST49
Zinc fingeri121 – 168Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST48
Zinc fingeri183 – 234Phorbol-ester/DAG-type 3PROSITE-ProRule annotationAdd BLAST52

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1169 Eukaryota
ENOG410XQVB LUCA
GeneTreeiENSGT00760000119050
HOGENOMiHOG000007900
HOVERGENiHBG094675
InParanoidiP52824
KOiK00901
OMAiTKPTFCH
OrthoDBiEOG091G02NN
PhylomeDBiP52824
TreeFamiTF312817

Family and domain databases

CDDicd00029 C1, 2 hits
Gene3Di3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR037607 DGK
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002219 PE/DAG-bd
IPR000159 RA_dom
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
PF00788 RA, 1 hit
SMARTiView protein in SMART
SM00109 C1, 3 hits
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SM00314 RA, 1 hit
SUPFAMiSSF111331 SSF111331, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
PS50200 RA, 1 hit
PS00479 ZF_DAG_PE_1, 3 hits
PS50081 ZF_DAG_PE_2, 3 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P52824-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAAAEPGAR AWLGGGSPRP GSPACSPVLG SGGRARPGPG PGPGPERAGV
60 70 80 90 100
RAPGPAAAPG HSFRKVTLTK PTFCHLCSDF IWGLAGFLCD VCNFMSHEKC
110 120 130 140 150
LKHVRIPCTS VAPSLVRVPV AHCFGPRGLH KRKFCAVCRK VLEAPALHCE
160 170 180 190 200
VCELHLHPDC VPFACSDCRQ CHQDGHQDHD THHHHWREGN LPSGARCEVC
210 220 230 240 250
RKTCGSSDVL AGVRCEWCGV QAHSLCSAAL APECGFGRLR SLVLPPACVR
260 270 280 290 300
LLPGGFSKTQ SFRIVEAAEP GEGGDGADGS AAVGPGRETQ ATPESGKQTL
310 320 330 340 350
KIFDGDDAVR RSQFRLVTVS RLAGAEEVLE AALRAHHIPE DPGHLELCRL
360 370 380 390 400
PPSSQACDAW AGGKAGSAVI SEEGRSPGSG EATPEAWVIR ALPRAQEVLK
410 420 430 440 450
IYPGWLKVGV AYVSVRVTPK STARSVVLEV LPLLGRQAES PESFQLVEVA
460 470 480 490 500
MGCRHVQRTM LMDEQPLLDR LQDIRQMSVR QVSQTRFYVA ESRDVAPHVS
510 520 530 540 550
LFVGGLPPGL SPEEYSSLLH EAGATKATVV SVSHIYSSQG AVVLDVACFA
560 570 580 590 600
EAERLYMLLK DMAVRGRLLT ALVLPDLLHA KLPPDSCPLL VFVNPKSGGL
610 620 630 640 650
KGRDLLCSFR KLLNPHQVFD LTNGGPLPGL HLFSQVPCFR VLVCGGDGTV
660 670 680 690 700
GWVLGALEET RYRLACPEPS VAILPLGTGN DLGRVLRWGA GYSGEDPFSV
710 720 730 740 750
LLSVDEADAV LMDRWTILLD AHEAGSAEND TADAEPPKIV QMSNYCGIGI
760 770 780 790 800
DAELSLDFHQ AREEEPGKFT SRLHNKGVYV RVGLQKISHS RSLHKQIRLQ
810 820 830 840 850
VERQEVELPS IEGLIFINIP SWGSGADLWG SDSDTRFEKP RMDDGLLEVV
860 870 880 890 900
GVTGVVHMGQ VQGGLRSGIR IAQGSYFRVT LLKATPVQVD GEPWVQAPGH
910 920 930 940
MIISAAGPKV HMLRKAKQKP RRAGTTRDAR ADAAPAPESD PR
Length:942
Mass (Da):101,155
Last modified:September 1, 2009 - v2
Checksum:i836D4FCBC208A5B6
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YA20H0YA20_HUMAN
Diacylglycerol kinase
DGKQ
876Annotation score:
D6RJB4D6RJB4_HUMAN
Diacylglycerol kinase theta
DGKQ
143Annotation score:
H0Y8Q7H0Y8Q7_HUMAN
Diacylglycerol kinase theta
DGKQ
157Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45 – 46PE → RD in AAA98749 (PubMed:7607687).Curated2
Sequence conflicti50 – 56VRAPGPA → GVRARAR in AAA98749 (PubMed:7607687).Curated7
Sequence conflicti933A → R in AAA98749 (PubMed:7607687).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05847827P → L1 PublicationCorresponds to variant dbSNP:rs17855876Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38707 mRNA Translation: AAA98749.1
AC019103 Genomic DNA No translation available.
BC063801 mRNA Translation: AAH63801.1
CCDSiCCDS3342.1
RefSeqiNP_001338.2, NM_001347.3
UniGeneiHs.584858

Genome annotation databases

EnsembliENST00000273814; ENSP00000273814; ENSG00000145214
GeneIDi1609
KEGGihsa:1609
UCSCiuc003gbw.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38707 mRNA Translation: AAA98749.1
AC019103 Genomic DNA No translation available.
BC063801 mRNA Translation: AAH63801.1
CCDSiCCDS3342.1
RefSeqiNP_001338.2, NM_001347.3
UniGeneiHs.584858

3D structure databases

ProteinModelPortaliP52824
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107979, 10 interactors
IntActiP52824, 1 interactor
STRINGi9606.ENSP00000273814

Chemistry databases

SwissLipidsiSLP:000000740

PTM databases

iPTMnetiP52824
PhosphoSitePlusiP52824

Polymorphism and mutation databases

BioMutaiDGKQ
DMDMi257051005

Proteomic databases

EPDiP52824
MaxQBiP52824
PaxDbiP52824
PeptideAtlasiP52824
PRIDEiP52824
ProteomicsDBi56542

Protocols and materials databases

DNASUi1609
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000273814; ENSP00000273814; ENSG00000145214
GeneIDi1609
KEGGihsa:1609
UCSCiuc003gbw.5 human

Organism-specific databases

CTDi1609
DisGeNETi1609
EuPathDBiHostDB:ENSG00000145214.13
GeneCardsiDGKQ
HGNCiHGNC:2856 DGKQ
HPAiCAB033835
HPA026797
MIMi601207 gene
neXtProtiNX_P52824
OpenTargetsiENSG00000145214
PharmGKBiPA27317
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1169 Eukaryota
ENOG410XQVB LUCA
GeneTreeiENSGT00760000119050
HOGENOMiHOG000007900
HOVERGENiHBG094675
InParanoidiP52824
KOiK00901
OMAiTKPTFCH
OrthoDBiEOG091G02NN
PhylomeDBiP52824
TreeFamiTF312817

Enzyme and pathway databases

ReactomeiR-HSA-114508 Effects of PIP2 hydrolysis

Miscellaneous databases

GeneWikiiDGKQ
GenomeRNAii1609
PROiPR:P52824
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000145214 Expressed in 177 organ(s), highest expression level in right hemisphere of cerebellum
CleanExiHS_DGKQ
ExpressionAtlasiP52824 baseline and differential
GenevisibleiP52824 HS

Family and domain databases

CDDicd00029 C1, 2 hits
Gene3Di3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR037607 DGK
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002219 PE/DAG-bd
IPR000159 RA_dom
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
PF00788 RA, 1 hit
SMARTiView protein in SMART
SM00109 C1, 3 hits
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SM00314 RA, 1 hit
SUPFAMiSSF111331 SSF111331, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
PS50200 RA, 1 hit
PS00479 ZF_DAG_PE_1, 3 hits
PS50081 ZF_DAG_PE_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiDGKQ_HUMAN
AccessioniPrimary (citable) accession number: P52824
Secondary accession number(s): Q6P3W4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 1, 2009
Last modified: October 10, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
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