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Protein

Hexokinase-3

Gene

HK3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.

Pathwayi: hexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi95 – 100ATPSequence analysis6
Nucleotide bindingi542 – 547ATPSequence analysis6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS08548-MONOMER
ReactomeiR-HSA-6798695 Neutrophil degranulation
R-HSA-70171 Glycolysis
SABIO-RKiP52790
UniPathwayiUPA00242

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-3 (EC:2.7.1.1)
Alternative name(s):
Hexokinase type III
Short name:
HK III
Gene namesi
Name:HK3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000160883.10
HGNCiHGNC:4925 HK3
MIMi142570 gene
neXtProtiNX_P52790

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi3101
OpenTargetsiENSG00000160883
PharmGKBiPA29303

Chemistry databases

ChEMBLiCHEMBL2709

Polymorphism and mutation databases

BioMutaiHK3
DMDMi206729871

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001975901 – 923Hexokinase-3Add BLAST923

Proteomic databases

EPDiP52790
MaxQBiP52790
PaxDbiP52790
PeptideAtlasiP52790
PRIDEiP52790
ProteomicsDBi56534

PTM databases

iPTMnetiP52790
PhosphoSitePlusiP52790
SwissPalmiP52790

Expressioni

Gene expression databases

BgeeiENSG00000160883
CleanExiHS_HK3
ExpressionAtlasiP52790 baseline and differential
GenevisibleiP52790 HS

Organism-specific databases

HPAiHPA056743

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi109348, 19 interactors
IntActiP52790, 7 interactors
MINTiP52790
STRINGi9606.ENSP00000292432

Structurei

Secondary structure

1923
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi480 – 487Combined sources8
Helixi488 – 490Combined sources3
Helixi494 – 513Combined sources20
Beta strandi536 – 566Combined sources31
Helixi570 – 573Combined sources4
Helixi577 – 595Combined sources19
Beta strandi604 – 608Combined sources5
Helixi639 – 649Combined sources11
Beta strandi657 – 661Combined sources5
Helixi663 – 672Combined sources10
Beta strandi678 – 695Combined sources18
Helixi696 – 698Combined sources3
Beta strandi706 – 712Combined sources7
Helixi715 – 717Combined sources3
Turni718 – 727Combined sources10
Helixi730 – 737Combined sources8
Beta strandi739 – 741Combined sources3
Helixi748 – 750Combined sources3
Helixi753 – 769Combined sources17
Helixi774 – 776Combined sources3
Helixi780 – 783Combined sources4
Helixi790 – 796Combined sources7
Helixi803 – 811Combined sources9
Turni812 – 814Combined sources3
Helixi819 – 854Combined sources36
Beta strandi858 – 867Combined sources10
Helixi869 – 873Combined sources5
Helixi877 – 888Combined sources12
Beta strandi892 – 898Combined sources7
Helixi903 – 918Combined sources16

3D structure databases

ProteinModelPortaliP52790
SMRiP52790
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52790

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 471Hexokinase 1PROSITE-ProRule annotationAdd BLAST445
Domaini477 – 912Hexokinase 2PROSITE-ProRule annotationAdd BLAST436

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 488RegulatoryAdd BLAST488
Regioni84 – 220Hexokinase small subdomain 1PROSITE-ProRule annotationAdd BLAST137
Regioni162 – 188Glucose-bindingSequence analysisAdd BLAST27
Regioni221 – 460Hexokinase large subdomain 1PROSITE-ProRule annotationAdd BLAST240
Regioni489 – 923CatalyticAdd BLAST435
Regioni531 – 661Hexokinase small subdomain 2PROSITE-ProRule annotationAdd BLAST131
Regioni603 – 629Glucose-bindingSequence analysisAdd BLAST27
Regioni662 – 901Hexokinase large subdomain 2PROSITE-ProRule annotationAdd BLAST240

Domaini

The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1369 Eukaryota
COG5026 LUCA
GeneTreeiENSGT00390000017159
HOGENOMiHOG000162671
HOVERGENiHBG005020
InParanoidiP52790
KOiK00844
OMAiVGTMMGC
OrthoDBiEOG091G08MD
PhylomeDBiP52790
TreeFamiTF314238

Family and domain databases

InterProiView protein in InterPro
IPR001312 Hexokinase
IPR019807 Hexokinase_BS
IPR022673 Hexokinase_C
IPR022672 Hexokinase_N
PANTHERiPTHR19443 PTHR19443, 4 hits
PfamiView protein in Pfam
PF00349 Hexokinase_1, 2 hits
PF03727 Hexokinase_2, 2 hits
PROSITEiView protein in PROSITE
PS00378 HEXOKINASE_1, 1 hit
PS51748 HEXOKINASE_2, 2 hits

Sequencei

Sequence statusi: Complete.

P52790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSIGSSGLR QGEETLSCSE EGLPGPSDSS ELVQECLQQF KVTRAQLQQI
60 70 80 90 100
QASLLGSMEQ ALRGQASPAP AVRMLPTYVG STPHGTEQGD FVVLELGATG
110 120 130 140 150
ASLRVLWVTL TGIEGHRVEP RSQEFVIPQE VMLGAGQQLF DFAAHCLSEF
160 170 180 190 200
LDAQPVNKQG LQLGFSFSFP CHQTGLDRST LISWTKGFRC SGVEGQDVVQ
210 220 230 240 250
LLRDAIRRQG AYNIDVVAVV NDTVGTMMGC EPGVRPCEVG LVVDTGTNAC
260 270 280 290 300
YMEEARHVAV LDEDRGRVCV SVEWGSFSDD GALGPVLTTF DHTLDHESLN
310 320 330 340 350
PGAQRFEKMI GGLYLGELVR LVLAHLARCG VLFGGCTSPA LLSQGSILLE
360 370 380 390 400
HVAEMEDPST GAARVHAILQ DLGLSPGASD VELVQHVCAA VCTRAAQLCA
410 420 430 440 450
AALAAVLSCL QHSREQQTLQ VAVATGGRVC ERHPRFCSVL QGTVMLLAPE
460 470 480 490 500
CDVSLIPSVD GGGRGVAMVT AVAARLAAHR RLLEETLAPF RLNHDQLAAV
510 520 530 540 550
QAQMRKAMAK GLRGEASSLR MLPTFVRATP DGSERGDFLA LDLGGTNFRV
560 570 580 590 600
LLVRVTTGVQ ITSEIYSIPE TVAQGSGQQL FDHIVDCIVD FQQKQGLSGQ
610 620 630 640 650
SLPLGFTFSF PCRQLGLDQG ILLNWTKGFK ASDCEGQDVV SLLREAITRR
660 670 680 690 700
QAVELNVVAI VNDTVGTMMS CGYEDPRCEI GLIVGTGTNA CYMEELRNVA
710 720 730 740 750
GVPGDSGRMC INMEWGAFGD DGSLAMLSTR FDASVDQASI NPGKQRFEKM
760 770 780 790 800
ISGMYLGEIV RHILLHLTSL GVLFRGQQIQ RLQTRDIFKT KFLSEIESDS
810 820 830 840 850
LALRQVRAIL EDLGLPLTSD DALMVLEVCQ AVSQRAAQLC GAGVAAVVEK
860 870 880 890 900
IRENRGLEEL AVSVGVDGTL YKLHPRFSSL VAATVRELAP RCVVTFLQSE
910 920
DGSGKGAALV TAVACRLAQL TRV
Length:923
Mass (Da):99,025
Last modified:September 23, 2008 - v2
Checksum:iDA75F2A9DBB895FF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti277F → L in AAC50732 (PubMed:8812439).Curated1
Sequence conflicti420Q → L in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti431E → Q in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti439V → I in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti510K → R in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti512 – 513LR → SE in AAC50422 (PubMed:8717435).Curated2
Sequence conflicti516A → S in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti519L → S in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti530 – 531PD → LT in AAC50422 (PubMed:8717435).Curated2
Sequence conflicti577 – 578GQ → AE in AAC50422 (PubMed:8717435).Curated2
Sequence conflicti604L → T in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti708R → H in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti718F → L in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti728S → R in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti750M → I in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti831A → V in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti836A → P in AAC50422 (PubMed:8717435).Curated1
Sequence conflicti853E → G in AAC50732 (PubMed:8812439).Curated1
Sequence conflicti918A → T in AAC50422 (PubMed:8717435).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_034004281G → R. Corresponds to variant dbSNP:rs35610191Ensembl.1
Natural variantiVAR_036186480R → W in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs376532514Ensembl.1
Natural variantiVAR_036187499A → V in a breast cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs755546220Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51333 mRNA Translation: AAC50732.1
CH471195 Genomic DNA Translation: EAW85048.1
BC028129 mRNA Translation: AAH28129.1
U42303 mRNA Translation: AAC50422.1
CCDSiCCDS4407.1
RefSeqiNP_002106.2, NM_002115.2
UniGeneiHs.411695

Genome annotation databases

EnsembliENST00000292432; ENSP00000292432; ENSG00000160883
GeneIDi3101
KEGGihsa:3101
UCSCiuc003mfa.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiHXK3_HUMAN
AccessioniPrimary (citable) accession number: P52790
Secondary accession number(s): Q8N1E7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 23, 2008
Last modified: July 18, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

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