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UniProtKB - P52789 (HXK2_HUMAN)

Entry version 207 (29 Sep 2021)
Sequence version 2 (07 Dec 2004)
Previous versions | rss
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Protein

Hexokinase-2

Gene

HK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed:23185017, PubMed:26985301, PubMed:29298880).

Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:29298880).

Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed:18350175).

4 Publications

Caution

Hexokinase is known to act as a monomer in normal conditions (By similarity). It however homodimerizes at elevated protein concentrations used for crystallizations (PubMed:26985301, PubMed:29298880).By similarity2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Hexokinase activity is specifically inhibited by 2,6-disubstituted glucosamines.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=0.21 mM for D-glucose1 Publication
  2. KM=1.13 mM for ATP1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: hexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.1 Publication This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei30ATP 1By similarity1
Binding sitei209Glucose-6-phosphate 1Combined sources1 Publication1
Binding sitei232Glucose-6-phosphate 1Combined sources1 Publication1
Binding sitei235Substrate 1Combined sources1 Publication1
Binding sitei260Substrate 1Combined sources1 Publication1
Binding sitei449Glucose-6-phosphate 1Combined sources1 Publication1
Binding sitei657Glucose-6-phosphate 2Combined sources2 Publications1
Binding sitei680ATP 2By similarity1
Binding sitei680Glucose-6-phosphate 2Combined sources2 Publications1
Binding sitei708Substrate 2Combined sources1 Publication1
Binding sitei897Glucose-6-phosphate 2Combined sources2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi84 – 89ATP 1By similarity6
Nucleotide bindingi425 – 426ATP 1By similarity2
Nucleotide bindingi532 – 537ATP 2By similarity6
Nucleotide bindingi747 – 748ATP 2By similarity2
Nucleotide bindingi784 – 788ATP 2By similarity5
Nucleotide bindingi863 – 867ATP 2By similarity5

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		MetaCyc:HS08399-MONOMER

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.7.1.1, 2681
2.7.1.2, 2681

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		P52789

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-70171, Glycolysis

SABIO-RK: Biochemical Reaction Kinetics Database

More...SABIO-RKi		P52789

SIGNOR Signaling Network Open Resource

More...SIGNORi		P52789

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00109;UER00180
UPA00242

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Hexokinase-2Curated (EC:2.7.1.13 Publications)
Alternative name(s):
Hexokinase type II2 Publications
Short name:
HK II2 Publications
Hexokinase-BBy similarity
Muscle form hexokinase1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:HK2Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 2

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:4923, HK2

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		601125, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_P52789

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000159399

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi209D → A: Decreased hexokinase activity. 1 Publication1
Mutagenesisi468R → A: Induces a rapid dissociation of D-glucose. 1 Publication1
Mutagenesisi657D → A: Decreased hexokinase activity. 1 Publication1

Organism-specific databases

DisGeNET

More...DisGeNETi		3099

Open Targets

More...OpenTargetsi		ENSG00000159399

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA29301

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		P52789, Tchem

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL2640

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		HK2

Domain mapping of disease mutations (DMDM)

More...DMDMi		56405344

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001975871 – 917Hexokinase-2Add BLAST917

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		P52789

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P52789

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		P52789

MaxQB - The MaxQuant DataBase

More...MaxQBi		P52789

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P52789

PeptideAtlas

More...PeptideAtlasi		P52789

PRoteomics IDEntifications database

More...PRIDEi		P52789

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		56533

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

More...GlyGeni		P52789, 1 site, 1 O-linked glycan (1 site)

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P52789

MetOSite database of methionine sulfoxide sites

More...MetOSitei		P52789

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		P52789

SwissPalm database of S-palmitoylation events

More...SwissPalmi		P52789

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000159399, Expressed in colon and 232 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		P52789, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		P52789, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000159399, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer (By similarity).

Interacts with TIGAR; the interaction increases hexokinase activity in a hypoxia- and HIF1A-dependent manner (PubMed:23185017).

By similarity1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		109346, 83 interactors

Database of interacting proteins

More...DIPi		DIP-50110N

Protein interaction database and analysis system

More...IntActi		P52789, 34 interactors

Molecular INTeraction database

More...MINTi		P52789

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000290573

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		P52789

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P52789, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1917
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P52789

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P52789

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini16 – 458Hexokinase 1PROSITE-ProRule annotationAdd BLAST443
Domaini464 – 906Hexokinase 2PROSITE-ProRule annotationAdd BLAST443

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 16Mitochondrial-binding peptide (MBP)1 PublicationAdd BLAST16
Regioni73 – 207Hexokinase small subdomain 1PROSITE-ProRule annotationAdd BLAST135
Regioni84 – 88Glucose-6-phosphate 1 bindingCombined sources1 Publication5
Regioni155 – 156Substrate 1 bindingCombined sources1 Publication2
Regioni172 – 173Substrate 1 bindingCombined sources1 Publication2
Regioni208 – 447Hexokinase large subdomain 1PROSITE-ProRule annotationAdd BLAST240
Regioni208 – 209Substrate 1 bindingCombined sources1 Publication2
Regioni291 – 294Substrate 1 bindingCombined sources1 Publication4
Regioni413 – 415Glucose-6-phosphate 1 bindingCombined sources1 Publication3
Regioni521 – 655Hexokinase small subdomain 2PROSITE-ProRule annotationAdd BLAST135
Regioni532 – 536Glucose-6-phosphate 2 bindingCombined sources2 Publications5
Regioni603 – 604Substrate 2 bindingCombined sources1 Publication2
Regioni620 – 621Substrate 2 bindingCombined sources1 Publication2
Regioni656 – 895Hexokinase large subdomain 2PROSITE-ProRule annotationAdd BLAST240
Regioni656 – 657Substrate 2 bindingCombined sources1 Publication2
Regioni682 – 683Substrate 2 bindingCombined sources1 Publication2
Regioni739 – 742Substrate 2 bindingCombined sources1 Publication4
Regioni861 – 863Glucose-6-phosphate 2 bindingCombined sources2 Publications3

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The N- and C-terminal halves of the protein contain a hexokinase domain (PubMed:29298880). In contrast to hexokinase-1 and -3 (HK1 and HK3, respectively), both hexokinase domains display catalytic activity (PubMed:29298880). The region connecting the two hexokinase domains is required for the catalytic activity of the N-terminal hexokinase domain (PubMed:29298880). The N-terminal half regulates stability of the whole enzyme (PubMed:29298880).1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG1369, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00950000182787

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P52789

Identification of Orthologs from Complete Genome Data

More...OMAi		MEEMRYI

Database of Orthologous Groups

More...OrthoDBi		1153545at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		P52789

TreeFam database of animal gene trees

More...TreeFami		TF314238

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR043129, ATPase_NBD
IPR001312, Hexokinase
IPR019807, Hexokinase_BS
IPR022673, Hexokinase_C
IPR022672, Hexokinase_N

The PANTHER Classification System

More...PANTHERi		PTHR19443, PTHR19443, 2 hits

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00349, Hexokinase_1, 2 hits
PF03727, Hexokinase_2, 2 hits

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF53067, SSF53067, 4 hits

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00378, HEXOKINASE_1, 2 hits
PS51748, HEXOKINASE_2, 2 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P52789-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIASHLLAYF FTELNHDQVQ KVDQYLYHMR LSDETLLEIS KRFRKEMEKG 
        60         70         80         90        100
LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLWVKVTDN 
       110        120        130        140        150
GLQKVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKDKKLPL 
       160        170        180        190        200
GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVALIR KAIQRRGDFD 
       210        220        230        240        250
IDIVAVVNDT VGTMMTCGYD DHNCEIGLIV GTGSNACYME EMRHIDMVEG 
       260        270        280        290        300
DEGRMCINME WGAFGDDGSL NDIRTEFDQE IDMGSLNPGK QLFEKMISGM 
       310        320        330        340        350
YMGELVRLIL VKMAKEELLF GGKLSPELLN TGRFETKDIS DIEGEKDGIR 
       360        370        380        390        400
KAREVLMRLG LDPTQEDCVA THRICQIVST RSASLCAATL AAVLQRIKEN 
       410        420        430        440        450
KGEERLRSTI GVDGSVYKKH PHFAKRLHKT VRRLVPGCDV RFLRSEDGSG 
       460        470        480        490        500
KGAAMVTAVA YRLADQHRAR QKTLEHLQLS HDQLLEVKRR MKVEMERGLS 
       510        520        530        540        550
KETHASAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKW 
       560        570        580        590        600
GGVEMHNKIY AIPQEVMHGT GDELFDHIVQ CIADFLEYMG MKGVSLPLGF 
       610        620        630        640        650
TFSFPCQQNS LDESILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD 
       660        670        680        690        700
VVAVVNDTVG TMMTCGFEDP HCEVGLIVGT GSNACYMEEM RNVELVEGEE 
       710        720        730        740        750
GRMCVNMEWG AFGDNGCLDD FRTEFDVAVD ELSLNPGKQR FEKMISGMYL 
       760        770        780        790        800
GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV 
       810        820        830        840        850
RAILQHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDRIRENRG 
       860        870        880        890        900
LDALKVTVGV DGTLYKLHPH FAKVMHETVK DLAPKCDVSF LQSEDGSGKG 
       910 
AALITAVACR IREAGQR
Length:917
Mass (Da):102,380
Last modified:December 7, 2004 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iF17CE1938CF13880
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PB90E9PB90_HUMAN
Hexokinase
HK2
889Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti803I → T in CAA86511 (PubMed:8250948).Curated1
Sequence conflicti870Missing in CAA86476 (PubMed:8786021).Curated1

<p>This subsection of the 'Sequence' section provides information on polymorphic variants. If the variant is associated with a disease state, the description of the latter can be found in the <a href="http://www.uniprot.org/manual/involvement_in_disease">'Involvement in disease'</a> subsection.<p><a href='/help/polymorphism' target='_top'>More...</a></p>Polymorphismi

Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease (PubMed:7883122, PubMed:7883123).2 Publications

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_003691142Q → H Does not affect activity. 4 PublicationsCorresponds to variant dbSNP:rs2229621Ensembl.1
Natural variantiVAR_010577148L → F1 Publication1
Natural variantiVAR_020504274R → C1 PublicationCorresponds to variant dbSNP:rs28363006Ensembl.1
Natural variantiVAR_020505314A → P1 PublicationCorresponds to variant dbSNP:rs28363015Ensembl.1
Natural variantiVAR_010578314A → V1 Publication1
Natural variantiVAR_020506331T → I1 PublicationCorresponds to variant dbSNP:rs28363016Ensembl.1
Natural variantiVAR_010579353R → C1 PublicationCorresponds to variant dbSNP:rs61748096Ensembl.1
Natural variantiVAR_020507387A → S1 PublicationCorresponds to variant dbSNP:rs28363029Ensembl.1
Natural variantiVAR_010580497R → Q1 PublicationCorresponds to variant dbSNP:rs145124653Ensembl.1
Natural variantiVAR_010581775R → Q1 PublicationCorresponds to variant dbSNP:rs185927605Ensembl.1
Natural variantiVAR_020508801R → Q1 PublicationCorresponds to variant dbSNP:rs28363057Ensembl.1
Natural variantiVAR_010582844R → K2 PublicationsCorresponds to variant dbSNP:rs2229629Ensembl.1
Natural variantiVAR_020509881D → N1 PublicationCorresponds to variant dbSNP:rs28363065Ensembl.1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
Z46376 mRNA Translation: CAA86511.1
Z46354 Z46369 Genomic DNA Translation: CAA86476.2
AF148513 mRNA Translation: AAD30174.1
AY623118 Genomic DNA Translation: AAT38114.1
CH471053 Genomic DNA Translation: EAW99601.1
CH471053 Genomic DNA Translation: EAW99602.1
BC021116 mRNA Translation: AAH21116.1
BC064369 mRNA Translation: AAH64369.1
D25412 Genomic DNA Translation: BAA04999.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS1956.1

Protein sequence database of the Protein Information Resource

More...PIRi		S48809, JC2025

NCBI Reference Sequences

More...RefSeqi		NP_000180.2, NM_000189.4

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000290573; ENSP00000290573; ENSG00000159399

Database of genes from NCBI RefSeq genomes

More...GeneIDi		3099

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:3099

UCSC genome browser

More...UCSCi		uc002snd.4, human

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

NIEHS-SNPs
Wikipedia

Hexokinase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46376 mRNA Translation: CAA86511.1
Z46354 Z46369 Genomic DNA Translation: CAA86476.2
AF148513 mRNA Translation: AAD30174.1
AY623118 Genomic DNA Translation: AAT38114.1
CH471053 Genomic DNA Translation: EAW99601.1
CH471053 Genomic DNA Translation: EAW99602.1
BC021116 mRNA Translation: AAH21116.1
BC064369 mRNA Translation: AAH64369.1
D25412 Genomic DNA Translation: BAA04999.1
CCDSiCCDS1956.1
PIRiS48809, JC2025
RefSeqiNP_000180.2, NM_000189.4

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NZTX-ray2.45A/B17-916[»]
5HEXX-ray2.73A/B17-917[»]
5HFUX-ray2.92A/B17-917[»]
5HG1X-ray2.76A17-916[»]
SMRiP52789
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi109346, 83 interactors
DIPiDIP-50110N
IntActiP52789, 34 interactors
MINTiP52789
STRINGi9606.ENSP00000290573

Chemistry databases

BindingDBiP52789
ChEMBLiCHEMBL2640

PTM databases

GlyGeniP52789, 1 site, 1 O-linked glycan (1 site)
iPTMnetiP52789
MetOSiteiP52789
PhosphoSitePlusiP52789
SwissPalmiP52789

Genetic variation databases

BioMutaiHK2
DMDMi56405344

Proteomic databases

EPDiP52789
jPOSTiP52789
MassIVEiP52789
MaxQBiP52789
PaxDbiP52789
PeptideAtlasiP52789
PRIDEiP52789
ProteomicsDBi56533

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		31617, 704 antibodies

The DNASU plasmid repository

More...DNASUi		3099

Genome annotation databases

EnsembliENST00000290573; ENSP00000290573; ENSG00000159399
GeneIDi3099
KEGGihsa:3099
UCSCiuc002snd.4, human

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		3099
DisGeNETi3099

GeneCards: human genes, protein and diseases

More...GeneCardsi		HK2
HGNCiHGNC:4923, HK2
HPAiENSG00000159399, Low tissue specificity
MIMi601125, gene
neXtProtiNX_P52789
OpenTargetsiENSG00000159399
PharmGKBiPA29301
VEuPathDBiHostDB:ENSG00000159399

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG1369, Eukaryota
GeneTreeiENSGT00950000182787
InParanoidiP52789
OMAiMEEMRYI
OrthoDBi1153545at2759
PhylomeDBiP52789
TreeFamiTF314238

Enzyme and pathway databases

UniPathwayiUPA00109;UER00180
UPA00242
BioCyciMetaCyc:HS08399-MONOMER
BRENDAi2.7.1.1, 2681
2.7.1.2, 2681
PathwayCommonsiP52789
ReactomeiR-HSA-70171, Glycolysis
SABIO-RKiP52789
SIGNORiP52789

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		3099, 80 hits in 1024 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		HK2, human
EvolutionaryTraceiP52789

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		HK2

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		3099
PharosiP52789, Tchem

Protein Ontology

More...PROi		PR:P52789
RNActiP52789, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000159399, Expressed in colon and 232 other tissues
ExpressionAtlasiP52789, baseline and differential
GenevisibleiP52789, HS

Family and domain databases

InterProiView protein in InterPro
IPR043129, ATPase_NBD
IPR001312, Hexokinase
IPR019807, Hexokinase_BS
IPR022673, Hexokinase_C
IPR022672, Hexokinase_N
PANTHERiPTHR19443, PTHR19443, 2 hits
PfamiView protein in Pfam
PF00349, Hexokinase_1, 2 hits
PF03727, Hexokinase_2, 2 hits
SUPFAMiSSF53067, SSF53067, 4 hits
PROSITEiView protein in PROSITE
PS00378, HEXOKINASE_1, 2 hits
PS51748, HEXOKINASE_2, 2 hits

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHXK2_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P52789
Secondary accession number(s): D6W5J2, Q8WU87, Q9UN82
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 7, 2004
Last modified: September 29, 2021
This is version 207 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families
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