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Protein

Gastric intrinsic factor

Gene

Gif

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171CobalaminBy similarity1
Binding sitei222CobalaminBy similarity1
Binding sitei270CobalaminBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCobalt transport, Ion transport, Transport
LigandCobalt

Enzyme and pathway databases

ReactomeiR-MMU-196741 Cobalamin (Cbl, vitamin B12) transport and metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Gastric intrinsic factor
Alternative name(s):
Intrinsic factor
Short name:
IF
Short name:
INF
Gene namesi
Name:Gif
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1202394 Gif

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000000555919 – 417Gastric intrinsic factorAdd BLAST399

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 246By similarity
Disulfide bondi103 ↔ 288By similarity
Disulfide bondi143 ↔ 182By similarity
Modified residuei191PhosphoserineBy similarity1
Glycosylationi311N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi330N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi413N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP52787
PaxDbiP52787
PRIDEiP52787

PTM databases

PhosphoSitePlusiP52787

Expressioni

Tissue specificityi

Gastric mucosa.

Gene expression databases

BgeeiENSMUSG00000024682 Expressed in 47 organ(s), highest expression level in stomach
CleanExiMM_GIF
GenevisibleiP52787 MM

Interactioni

Subunit structurei

Interacts with CUBN (via CUB domains).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025585

Structurei

3D structure databases

ProteinModelPortaliP52787
SMRiP52787
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni365 – 370Cobalamin bindingBy similarity6
Regioni386 – 395Cobalamin bindingBy similarity10

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IVBN Eukaryota
ENOG410XSU1 LUCA
GeneTreeiENSGT00530000063370
HOGENOMiHOG000013214
HOVERGENiHBG006133
InParanoidiP52787
KOiK14615
OMAiSVLIAMN
OrthoDBiEOG091G095C
PhylomeDBiP52787
TreeFamiTF333092

Family and domain databases

InterProiView protein in InterPro
IPR002157 Cbl-bd_prot
IPR027954 DUF4430
PfamiView protein in Pfam
PF01122 Cobalamin_bind, 1 hit
PF14478 DUF4430, 1 hit
PROSITEiView protein in PROSITE
PS00468 COBALAMIN_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52787-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAWLTLYLLS VLWAVAGTST RAQSSCSVPP DQQPWVDGLQ ALMENSVTDS
60 70 80 90 100
DFPNPSILIA MNLAGAYNVE AQKLLTYQLM ASDSADLTSG QLALTVMALT
110 120 130 140 150
SSCRDPGSKV STLLKKMENW SPSSPGAESS AFYGPGLAIL ALCQKSSEAT
160 170 180 190 200
LPIAVRFAKT LMMEPSPFNV DTGAVATLAL TCMYNKIPVG SQENYRDLFG
210 220 230 240 250
QALKAIVEKI SLRIKADGII GDIYSTGLAM QALSVTPEQP TKKWDCEKTM
260 270 280 290 300
HTILNEIKQG KFQNPMSIAQ ILPSLKGKTY LDVPQVTCGP DHEVPPTLTD
310 320 330 340 350
YPTPVPTSVS NITVIYTINN QLRGVDLLFN VTIEVSVKSG SVLLAVLEEA
360 370 380 390 400
QRKNSMFKFE TTMTSWGLIV SSINNIAENV NHKTYWEFLS GKTPLDEGVA
410
YYIPFNHEHI TANFTQY
Length:417
Mass (Da):45,497
Last modified:March 3, 2009 - v2
Checksum:iBF38663EFC90F34A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86D → N in AAA37881 (PubMed:8253786).Curated1
Sequence conflicti86D → N in AAA37882 (PubMed:8253786).Curated1
Sequence conflicti156R → A in AAA37881 (PubMed:8253786).Curated1
Sequence conflicti156R → A in AAA37882 (PubMed:8253786).Curated1
Sequence conflicti327L → P in AAA37881 (PubMed:8253786).Curated1
Sequence conflicti327L → P in AAA37882 (PubMed:8253786).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24192 Genomic DNA Translation: AAA37881.1
L24191 mRNA Translation: AAA37882.1
AK078933 mRNA Translation: BAC37468.1
CH466534 Genomic DNA Translation: EDL41439.1
BC118519 mRNA Translation: AAI18520.1
CCDSiCCDS29609.1
PIRiA49684
RefSeqiNP_032144.2, NM_008118.3
UniGeneiMm.456

Genome annotation databases

EnsembliENSMUST00000025585; ENSMUSP00000025585; ENSMUSG00000024682
GeneIDi14603
KEGGimmu:14603
UCSCiuc008gsw.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24192 Genomic DNA Translation: AAA37881.1
L24191 mRNA Translation: AAA37882.1
AK078933 mRNA Translation: BAC37468.1
CH466534 Genomic DNA Translation: EDL41439.1
BC118519 mRNA Translation: AAI18520.1
CCDSiCCDS29609.1
PIRiA49684
RefSeqiNP_032144.2, NM_008118.3
UniGeneiMm.456

3D structure databases

ProteinModelPortaliP52787
SMRiP52787
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025585

PTM databases

PhosphoSitePlusiP52787

Proteomic databases

MaxQBiP52787
PaxDbiP52787
PRIDEiP52787

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025585; ENSMUSP00000025585; ENSMUSG00000024682
GeneIDi14603
KEGGimmu:14603
UCSCiuc008gsw.1 mouse

Organism-specific databases

CTDi2694
MGIiMGI:1202394 Gif

Phylogenomic databases

eggNOGiENOG410IVBN Eukaryota
ENOG410XSU1 LUCA
GeneTreeiENSGT00530000063370
HOGENOMiHOG000013214
HOVERGENiHBG006133
InParanoidiP52787
KOiK14615
OMAiSVLIAMN
OrthoDBiEOG091G095C
PhylomeDBiP52787
TreeFamiTF333092

Enzyme and pathway databases

ReactomeiR-MMU-196741 Cobalamin (Cbl, vitamin B12) transport and metabolism

Miscellaneous databases

PROiPR:P52787
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024682 Expressed in 47 organ(s), highest expression level in stomach
CleanExiMM_GIF
GenevisibleiP52787 MM

Family and domain databases

InterProiView protein in InterPro
IPR002157 Cbl-bd_prot
IPR027954 DUF4430
PfamiView protein in Pfam
PF01122 Cobalamin_bind, 1 hit
PF14478 DUF4430, 1 hit
PROSITEiView protein in PROSITE
PS00468 COBALAMIN_BINDING, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIF_MOUSE
AccessioniPrimary (citable) accession number: P52787
Secondary accession number(s): Q8C5C1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 3, 2009
Last modified: November 7, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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