UniProtKB - P52758 (RIDA_HUMAN)
2-iminobutanoate/2-iminopropanoate deaminase
RIDA
Functioni
Catalyzes the hydrolytic deamination of enamine/imine intermediates that form during the course of normal metabolism. May facilitate the release of ammonia from these potentially toxic reactive metabolites, reducing their impact on cellular components. It may act on enamine/imine intermediates formed by several types of pyridoxal-5'-phosphate-dependent dehydratases including L-threonine dehydratase.
2 PublicationsAlso promotes endoribonucleolytic cleavage of some transcripts by promoting recruitment of the ribonuclease P/MRP complex (PubMed:8973653, PubMed:30930054).
Acts by bridging YTHDF2 and the ribonuclease P/MRP complex (PubMed:30930054).
RIDA/HRSP12 binds to N6-methyladenosine (m6A)-containing mRNAs containing a 5'-GGUUC-3' motif: cooperative binding of RIDA/HRSP12 and YTHDF2 to such transcripts lead to recruitment of the ribonuclease P/MRP complex and subsequent endoribonucleolytic cleavage (PubMed:30930054).
2 PublicationsCatalytic activityi
GO - Molecular functioni
- 2-iminobutanoate deaminase activity Source: UniProtKB
- 2-iminopropanoate deaminase activity Source: RHEA
- deaminase activity Source: GO_Central
- endoribonuclease activity, producing 3'-phosphomonoesters Source: UniProtKB
- identical protein binding Source: Ensembl
- long-chain fatty acid binding Source: Ensembl
- mRNA binding Source: UniProtKB
- RNA binding Source: UniProtKB
- transition metal ion binding Source: Ensembl
GO - Biological processi
- brain development Source: Ensembl
- G1 to G0 transition Source: Ensembl
- kidney development Source: Ensembl
- lipid metabolic process Source: UniProtKB-KW
- L-threonine catabolic process to glycine Source: Reactome
- lung development Source: Ensembl
- mRNA catabolic process Source: UniProtKB
- mRNA destabilization Source: UniProtKB
- negative regulation of epithelial cell proliferation Source: Ensembl
- negative regulation of translation Source: UniProtKB
- organonitrogen compound catabolic process Source: UniProtKB
- response to lipid Source: Ensembl
- response to salt Source: Ensembl
Keywordsi
Molecular function | Hydrolase, RNA-binding |
Biological process | Lipid metabolism |
Enzyme and pathway databases
PathwayCommonsi | P52758 |
Reactomei | R-HSA-8849175, Threonine catabolism |
SignaLinki | P52758 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-iminobutanoate/2-iminopropanoate deaminase1 Publication (EC:3.5.99.101 Publication)Alternative name(s): 14.5 kDa translational inhibitor protein1 Publication Short name: hp14.51 Publication Short name: p14.51 Publication Heat-responsive protein 12Imported Reactive intermediate imine deaminase A homologImported Translation inhibitor L-PSP ribonucleaseBy similarity UK114 antigen homolog1 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16897, RIDA |
MIMi | 602487, gene |
neXtProti | NX_P52758 |
VEuPathDBi | HostDB:ENSG00000132541 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Peroxisome
- Peroxisome By similarity
Nucleus
- Nucleus 1 Publication
Mitochondrion
- Mitochondrion By similarity
Note: Mostly cytoplasmic but, in less differentiated cells occasionally nuclear.1 Publication
Cytosol
- cytosol Source: GO_Central
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Mitochondrion
- mitochondrial matrix Source: UniProtKB
- mitochondrion Source: GO_Central
Nucleus
- nucleus Source: UniProtKB
Peroxisome
- peroxisome Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Mitochondrion, Nucleus, PeroxisomePathology & Biotechi
Organism-specific databases
DisGeNETi | 10247 |
OpenTargetsi | ENSG00000132541 |
PharmGKBi | PA134890258 |
Miscellaneous databases
Pharosi | P52758, Tbio |
Genetic variation databases
BioMutai | RIDA |
DMDMi | 1717975 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000170308 | 2 – 137 | 2-iminobutanoate/2-iminopropanoate deaminaseAdd BLAST | 136 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 13 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 60 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 67 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 74 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 136 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
CPTACi | CPTAC-387 CPTAC-388 |
EPDi | P52758 |
jPOSTi | P52758 |
MassIVEi | P52758 |
PaxDbi | P52758 |
PeptideAtlasi | P52758 |
PRIDEi | P52758 |
ProteomicsDBi | 56530 |
TopDownProteomicsi | P52758 |
2D gel databases
UCD-2DPAGEi | P52758 |
PTM databases
iPTMneti | P52758 |
PhosphoSitePlusi | P52758 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSG00000132541, Expressed in right lobe of liver and 219 other tissues |
ExpressionAtlasi | P52758, baseline and differential |
Genevisiblei | P52758, HS |
Organism-specific databases
HPAi | ENSG00000132541, Group enriched (kidney, liver) |
Interactioni
Subunit structurei
Binary interactionsi
P52758
With | #Exp. | IntAct |
---|---|---|
BANP - isoform 2 [Q8N9N5-2] | 3 | EBI-1045080,EBI-11524452 |
GO - Molecular functioni
- identical protein binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 115541, 28 interactors |
IntActi | P52758, 16 interactors |
STRINGi | 9606.ENSP00000254878 |
Miscellaneous databases
RNActi | P52758, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P52758 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P52758 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2317, Eukaryota |
GeneTreei | ENSGT00420000029792 |
HOGENOMi | CLU_100715_7_1_1 |
InParanoidi | P52758 |
OMAi | GSYFKEP |
OrthoDBi | 1435276at2759 |
PhylomeDBi | P52758 |
TreeFami | TF105775 |
Family and domain databases
Gene3Di | 3.30.1330.40, 1 hit |
InterProi | View protein in InterPro IPR006056, RidA IPR019897, RidA_CS IPR035959, RutC-like_sf IPR006175, YjgF/YER057c/UK114 |
PANTHERi | PTHR11803, PTHR11803, 1 hit |
Pfami | View protein in Pfam PF01042, Ribonuc_L-PSP, 1 hit |
SUPFAMi | SSF55298, SSF55298, 1 hit |
TIGRFAMsi | TIGR00004, TIGR00004, 1 hit |
PROSITEi | View protein in PROSITE PS01094, UPF0076, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSSLIRRVIS TAKAPGAIGP YSQAVLVDRT IYISGQIGMD PSSGQLVSGG
60 70 80 90 100
VAEEAKQALK NMGEILKAAG CDFTNVVKTT VLLADINDFN TVNEIYKQYF
110 120 130
KSNFPARAAY QVAALPKGSR IEIEAVAIQG PLTTASL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0YBX3 | H0YBX3_HUMAN | 2-iminobutanoate/2-iminopropanoate ... | RIDA | 127 | Annotation score: | ||
H0YB34 | H0YB34_HUMAN | 2-iminobutanoate/2-iminopropanoate ... | RIDA | 148 | Annotation score: | ||
E5RIP8 | E5RIP8_HUMAN | 2-iminobutanoate/2-iminopropanoate ... | RIDA | 42 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 84 | A → V in CAG46453 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X95384 mRNA Translation: CAA64670.1 AY026764 Genomic DNA Translation: AAK01939.1 CR456844 mRNA Translation: CAG33125.1 CR541652 mRNA Translation: CAG46453.1 CH471060 Genomic DNA Translation: EAW91774.1 BC010280 mRNA Translation: AAH10280.1 BC012592 mRNA Translation: AAH12592.1 BC093059 mRNA Translation: AAH93059.1 |
CCDSi | CCDS6276.1 |
RefSeqi | NP_005827.1, NM_005836.2 |
Genome annotation databases
Ensembli | ENST00000254878; ENSP00000254878; ENSG00000132541 |
GeneIDi | 10247 |
KEGGi | hsa:10247 |
MANE-Selecti | ENST00000254878.8; ENSP00000254878.3; NM_005836.3; NP_005827.1 |
UCSCi | uc003yii.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X95384 mRNA Translation: CAA64670.1 AY026764 Genomic DNA Translation: AAK01939.1 CR456844 mRNA Translation: CAG33125.1 CR541652 mRNA Translation: CAG46453.1 CH471060 Genomic DNA Translation: EAW91774.1 BC010280 mRNA Translation: AAH10280.1 BC012592 mRNA Translation: AAH12592.1 BC093059 mRNA Translation: AAH93059.1 |
CCDSi | CCDS6276.1 |
RefSeqi | NP_005827.1, NM_005836.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ONI | X-ray | 1.90 | A/B/C/D/E/F/G/H/I | 2-137 | [»] | |
SMRi | P52758 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115541, 28 interactors |
IntActi | P52758, 16 interactors |
STRINGi | 9606.ENSP00000254878 |
PTM databases
iPTMneti | P52758 |
PhosphoSitePlusi | P52758 |
Genetic variation databases
BioMutai | RIDA |
DMDMi | 1717975 |
2D gel databases
UCD-2DPAGEi | P52758 |
Proteomic databases
CPTACi | CPTAC-387 CPTAC-388 |
EPDi | P52758 |
jPOSTi | P52758 |
MassIVEi | P52758 |
PaxDbi | P52758 |
PeptideAtlasi | P52758 |
PRIDEi | P52758 |
ProteomicsDBi | 56530 |
TopDownProteomicsi | P52758 |
Protocols and materials databases
Antibodypediai | 13021, 165 antibodies from 24 providers |
DNASUi | 10247 |
Genome annotation databases
Ensembli | ENST00000254878; ENSP00000254878; ENSG00000132541 |
GeneIDi | 10247 |
KEGGi | hsa:10247 |
MANE-Selecti | ENST00000254878.8; ENSP00000254878.3; NM_005836.3; NP_005827.1 |
UCSCi | uc003yii.2, human |
Organism-specific databases
CTDi | 10247 |
DisGeNETi | 10247 |
GeneCardsi | RIDA |
HGNCi | HGNC:16897, RIDA |
HPAi | ENSG00000132541, Group enriched (kidney, liver) |
MIMi | 602487, gene |
neXtProti | NX_P52758 |
OpenTargetsi | ENSG00000132541 |
PharmGKBi | PA134890258 |
VEuPathDBi | HostDB:ENSG00000132541 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2317, Eukaryota |
GeneTreei | ENSGT00420000029792 |
HOGENOMi | CLU_100715_7_1_1 |
InParanoidi | P52758 |
OMAi | GSYFKEP |
OrthoDBi | 1435276at2759 |
PhylomeDBi | P52758 |
TreeFami | TF105775 |
Enzyme and pathway databases
PathwayCommonsi | P52758 |
Reactomei | R-HSA-8849175, Threonine catabolism |
SignaLinki | P52758 |
Miscellaneous databases
BioGRID-ORCSi | 10247, 6 hits in 1041 CRISPR screens |
ChiTaRSi | RIDA, human |
EvolutionaryTracei | P52758 |
GeneWikii | Heat-responsive_protein_12 |
GenomeRNAii | 10247 |
Pharosi | P52758, Tbio |
PROi | PR:P52758 |
RNActi | P52758, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000132541, Expressed in right lobe of liver and 219 other tissues |
ExpressionAtlasi | P52758, baseline and differential |
Genevisiblei | P52758, HS |
Family and domain databases
Gene3Di | 3.30.1330.40, 1 hit |
InterProi | View protein in InterPro IPR006056, RidA IPR019897, RidA_CS IPR035959, RutC-like_sf IPR006175, YjgF/YER057c/UK114 |
PANTHERi | PTHR11803, PTHR11803, 1 hit |
Pfami | View protein in Pfam PF01042, Ribonuc_L-PSP, 1 hit |
SUPFAMi | SSF55298, SSF55298, 1 hit |
TIGRFAMsi | TIGR00004, TIGR00004, 1 hit |
PROSITEi | View protein in PROSITE PS01094, UPF0076, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RIDA_HUMAN | |
Accessioni | P52758Primary (citable) accession number: P52758 Secondary accession number(s): Q6FHU9, Q6IBG0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 185 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families