RIDA

Functionⁱ

Catalyzes the hydrolytic deamination of enamine/imine intermediates that form during the course of normal metabolism. May facilitate the release of ammonia from these potentially toxic reactive metabolites, reducing their impact on cellular components. It may act on enamine/imine intermediates formed by several types of pyridoxal-5'-phosphate-dependent dehydratases including L-threonine dehydratase.2 Publications

May also function as an endoribonuclease, cleaving mRNA phosphodiester bonds of single-stranded RNA (By similarity). Thereby, may inhibit protein translation (PubMed:8973653).By similarity1 Publication

Catalytic activityⁱ

2-iminobutanoate + H₂O = 2-oxobutanoate + NH₃.1 Publication

2-iminopropanoate + H₂O = pyruvate + NH₃.1 Publication

GO - Molecular functionⁱ

deaminase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 22094463
endoribonuclease activity, producing 3'-phosphomonoesters Source: UniProtKB
long-chain fatty acid binding Source: Ensembl
platinum binding Source: Ensembl
protein homodimerization activity Source: Ensembl
RNA binding
Source: UniProtKB
Inferred from high throughput direct assayⁱ
- 22658674
transition metal ion binding Source: Ensembl
xenon atom binding Source: Ensembl

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

brain development Source: Ensembl
G1 to G0 transition Source: Ensembl
kidney development Source: Ensembl
L-threonine catabolic process to glycine Source: Reactome
lung development Source: Ensembl
negative regulation of epithelial cell proliferation Source: Ensembl
negative regulation of translation Source: UniProtKB
organonitrogen compound catabolic process
Source: UniProtKB
Inferred from direct assayⁱ
- 22094463
response to lipid Source: Ensembl
response to salt Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase

Molecular function

Hydrolase

Enzyme and pathway databases

Reactomeⁱ	R-HSA-8849175 Threonine catabolism

Reactomeⁱ

R-HSA-8849175 Threonine catabolism

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 2-iminobutanoate/2-iminopropanoate deaminase1 Publication (EC:3.5.99.101 Publication) Alternative name(s): 14.5 kDa translational inhibitor protein1 Publication Short name: hp14.51 Publication Short name: p14.51 Publication Heat-responsive protein 12Imported Reactive intermediate imine deaminase A homologImported Translation inhibitor L-PSP ribonucleaseBy similarity (EC:3.1.-.-By similarity) UK114 antigen homolog1 Publication
Gene namesⁱ	Name:RIDAImported Synonyms:HRSP12Imported
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 8

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000132541.10
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:16897 RIDA
MIMⁱ	602487 gene
neXtProtⁱ	NX_P52758

Keywords - Cellular componentⁱ

Cytoplasm, Mitochondrion, Nucleus, Peroxisome

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	10247
Open Targets More...OpenTargetsⁱ	ENSG00000132541
PharmGKBⁱ	PA134890258

Chemistry databases

Drug and drug target database More...DrugBankⁱ	DB03793 Benzoic Acid

DrugBankⁱ

DB03793 Benzoic Acid

Polymorphism and mutation databases

BioMutaⁱ	HRSP12
DMDMⁱ	1717975

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCombined sources
ChainⁱPRO_0000170308	2 – 137	2-iminobutanoate/2-iminopropanoate deaminaseAdd BLAST		136

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylserineCombined sources	1
Modified residueⁱ	13	N6-succinyllysineBy similarity	1
Modified residueⁱ	60	N6-succinyllysineBy similarity	1
Modified residueⁱ	67	N6-succinyllysineBy similarity	1
Modified residueⁱ	74	PhosphothreonineCombined sources	1
Modified residueⁱ	136	PhosphoserineCombined sources	1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	P52758
PaxDbⁱ	P52758
PeptideAtlas More...PeptideAtlasⁱ	P52758
PRIDEⁱ	P52758
ProteomicsDBⁱ	56530
TopDownProteomicsⁱ	P52758

2D gel databases

UCD-2DPAGEⁱ	P52758

UCD-2DPAGEⁱ

P52758

PTM databases

iPTMnetⁱ	P52758
PhosphoSitePlusⁱ	P52758

Expressionⁱ

Tissue specificityⁱ

Expressed predominantly in liver and kidney. Lower levels in lung and brain.2 Publications

Developmental stageⁱ

Up-regulated during cellular differentiation.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000132541 Expressed in 205 organ(s), highest expression level in right lobe of liver
CleanExⁱ	HS_HRSP12
ExpressionAtlasⁱ	P52758 baseline and differential
Genevisibleⁱ	P52758 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA022856 HPA023489

HPAⁱ

HPA022856
HPA023489

Interactionⁱ

Subunit structureⁱ

Homotrimer.1 Publication

GO - Molecular functionⁱ

protein homodimerization activity Source: Ensembl

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	115541, 25 interactors
IntActⁱ	P52758, 11 interactors
STRINGⁱ	9606.ENSP00000254878

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	4 – 9	Combined sources	6
Beta strandⁱ	22 – 27	Combined sources	6
Beta strandⁱ	30 – 36	Combined sources	7
Turnⁱ	41 – 43	Combined sources	3
Beta strandⁱ	48 – 50	Combined sources	3
Helixⁱ	51 – 68	Combined sources	18
Helixⁱ	73 – 75	Combined sources	3
Beta strandⁱ	76 – 84	Combined sources	9
Helixⁱ	86 – 88	Combined sources	3
Helixⁱ	89 – 99	Combined sources	11
Beta strandⁱ	106 – 111	Combined sources	6
Helixⁱ	116 – 118	Combined sources	3
Beta strandⁱ	120 – 128	Combined sources	9
Beta strandⁱ	132 – 135	Combined sources	4

Show more details

3D structure databases

ProteinModelPortalⁱ	P52758
SMRⁱ	P52758
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P52758

EvolutionaryTraceⁱ

P52758

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the RutC family.Curated

Phylogenomic databases

eggNOGⁱ	KOG2317 Eukaryota COG0251 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00420000029792
HOGENOMⁱ	HOG000267215
HOVERGENⁱ	HBG003597
InParanoidⁱ	P52758
KEGG Orthology (KO) More...KOⁱ	K09022
PhylomeDBⁱ	P52758
TreeFamⁱ	TF105775

Family and domain databases

Gene3Dⁱ	3.30.1330.40, 1 hit
InterProⁱ	View protein in InterPro IPR006056 RidA IPR019897 RidA_CS IPR035959 RutC-like_sf IPR006175 YjgF/YER057c/UK114
PANTHERⁱ	PTHR11803 PTHR11803, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01042 Ribonuc_L-PSP, 1 hit
SUPFAMⁱ	SSF55298 SSF55298, 1 hit
TIGRFAMsⁱ	TIGR00004 TIGR00004, 1 hit
PROSITEⁱ	View protein in PROSITE PS01094 UPF0076, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show all Align All

P52758-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSSLIRRVIS TAKAPGAIGP YSQAVLVDRT IYISGQIGMD PSSGQLVSGG 
        60         70         80         90        100
VAEEAKQALK NMGEILKAAG CDFTNVVKTT VLLADINDFN TVNEIYKQYF 
       110        120        130 
KSNFPARAAY QVAALPKGSR IEIEAVAIQG PLTTASL

Length:137

Mass (Da):14,494

Last modified:October 1, 1996 - v1

Checksum:ⁱDD0740621E8BE6AD

GO

Computationally mapped potential isoform sequencesⁱ

There are 3 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

H0YBX3	H0YBX3_HUMAN	2-iminobutanoate/2-iminopropanoate ... 2-iminobutanoate/2-iminopropanoate deaminase	RIDA	127	Annotation score:
H0YB34	H0YB34_HUMAN	2-iminobutanoate/2-iminopropanoate ... 2-iminobutanoate/2-iminopropanoate deaminase	RIDA	148	Annotation score:
E5RIP8	E5RIP8_HUMAN	2-iminobutanoate/2-iminopropanoate ... 2-iminobutanoate/2-iminopropanoate deaminase	RIDA	42	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	84	A → V in CAG46453 (Ref. 3) Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X95384 mRNA Translation: CAA64670.1 AY026764 Genomic DNA Translation: AAK01939.1 CR456844 mRNA Translation: CAG33125.1 CR541652 mRNA Translation: CAG46453.1 CH471060 Genomic DNA Translation: EAW91774.1 BC010280 mRNA Translation: AAH10280.1 BC012592 mRNA Translation: AAH12592.1 BC093059 mRNA Translation: AAH93059.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS6276.1
NCBI Reference Sequences More...RefSeqⁱ	NP_005827.1, NM_005836.2
UniGeneⁱ	Hs.18426

Genome annotation databases

Ensemblⁱ	ENST00000254878; ENSP00000254878; ENSG00000132541
GeneIDⁱ	10247
KEGGⁱ	hsa:10247
UCSC genome browser More...UCSCⁱ	uc003yii.2 human

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P52758	Heat-responsive protein 12, isoform CRA_a		HUMAN		137	UniRef100_P52758
Uncharacterized protein		PAPAN		117

Protein	Similar proteins		Species	Score	Length	Source
P52758	Heat-responsive protein 12, isoform CRA_a		HUMAN		137	UniRef90_P52758
Uncharacterized protein		PAPAN		117
Uncharacterized protein		RHIBE		139
Uncharacterized protein		RHIRO		136
Ribonuclease UK114		CALJA		137
+40

Protein	Similar proteins		Species	Score	Length	Source
P52758	Heat-responsive protein 12, isoform CRA_a		HUMAN		137	UniRef50_P52758
Uncharacterized protein		PAPAN		117
Uncharacterized protein		RHIBE		139
Uncharacterized protein		RHIRO		136
Uncharacterized protein		CHLSB		137
+79

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X95384 mRNA Translation: CAA64670.1 AY026764 Genomic DNA Translation: AAK01939.1 CR456844 mRNA Translation: CAG33125.1 CR541652 mRNA Translation: CAG46453.1 CH471060 Genomic DNA Translation: EAW91774.1 BC010280 mRNA Translation: AAH10280.1 BC012592 mRNA Translation: AAH12592.1 BC093059 mRNA Translation: AAH93059.1
CCDSⁱ	CCDS6276.1
RefSeqⁱ	NP_005827.1, NM_005836.2
UniGeneⁱ	Hs.18426

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1ONI	X-ray	1.90	A/B/C/D/E/F/G/H/I	2-137	[»]
ProteinModelPortalⁱ	P52758
SMRⁱ	P52758
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	115541, 25 interactors
IntActⁱ	P52758, 11 interactors
STRINGⁱ	9606.ENSP00000254878

Chemistry databases

DrugBankⁱ	DB03793 Benzoic Acid

DrugBankⁱ

DB03793 Benzoic Acid

PTM databases

iPTMnetⁱ	P52758
PhosphoSitePlusⁱ	P52758

Polymorphism and mutation databases

BioMutaⁱ	HRSP12
DMDMⁱ	1717975

2D gel databases

UCD-2DPAGEⁱ	P52758

UCD-2DPAGEⁱ

P52758

Proteomic databases

EPDⁱ	P52758
PaxDbⁱ	P52758
PeptideAtlasⁱ	P52758
PRIDEⁱ	P52758
ProteomicsDBⁱ	56530
TopDownProteomicsⁱ	P52758

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	10247
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000254878; ENSP00000254878; ENSG00000132541
GeneIDⁱ	10247
KEGGⁱ	hsa:10247
UCSCⁱ	uc003yii.2 human

Organism-specific databases

CTDⁱ	10247
DisGeNETⁱ	10247
EuPathDBⁱ	HostDB:ENSG00000132541.10
GeneCardsⁱ	RIDA
HGNCⁱ	HGNC:16897 RIDA
HPAⁱ	HPA022856 HPA023489
MIMⁱ	602487 gene
neXtProtⁱ	NX_P52758
OpenTargetsⁱ	ENSG00000132541
PharmGKBⁱ	PA134890258
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG2317 Eukaryota COG0251 LUCA
GeneTreeⁱ	ENSGT00420000029792
HOGENOMⁱ	HOG000267215
HOVERGENⁱ	HBG003597
InParanoidⁱ	P52758
KOⁱ	K09022
PhylomeDBⁱ	P52758
TreeFamⁱ	TF105775

Enzyme and pathway databases

Reactomeⁱ	R-HSA-8849175 Threonine catabolism

Reactomeⁱ

R-HSA-8849175 Threonine catabolism

Miscellaneous databases

ChiTaRSⁱ	RIDA human
EvolutionaryTraceⁱ	P52758
GeneWikiⁱ	Heat-responsive_protein_12
GenomeRNAiⁱ	10247
Protein Ontology More...PROⁱ	PR:P52758
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000132541 Expressed in 205 organ(s), highest expression level in right lobe of liver
CleanExⁱ	HS_HRSP12
ExpressionAtlasⁱ	P52758 baseline and differential
Genevisibleⁱ	P52758 HS

Family and domain databases

Gene3Dⁱ	3.30.1330.40, 1 hit
InterProⁱ	View protein in InterPro IPR006056 RidA IPR019897 RidA_CS IPR035959 RutC-like_sf IPR006175 YjgF/YER057c/UK114
PANTHERⁱ	PTHR11803 PTHR11803, 1 hit
Pfamⁱ	View protein in Pfam PF01042 Ribonuc_L-PSP, 1 hit
SUPFAMⁱ	SSF55298 SSF55298, 1 hit
TIGRFAMsⁱ	TIGR00004 TIGR00004, 1 hit
PROSITEⁱ	View protein in PROSITE PS01094 UPF0076, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RIDA_HUMAN
Accessionⁱ	P52758Primary (citable) accession number: P52758 Secondary accession number(s): Q6FHU9, Q6IBG0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
	Last sequence update:	October 1, 1996
	Last modified:	November 7, 2018
	This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM

UniProtKB

UniParc

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Supporting data

UniProtKB - P52758 (RIDA_HUMAN)

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2-iminobutanoate/2-iminopropanoate deaminase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Mitochondrion

Peroxisome

Other locations

Cytosol

Extracellular region or secreted

Mitochondrion

Nucleus

Peroxisome

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

2D gel databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Polymorphism and mutation databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ