UniProtKB - P52734 (FGD1_MOUSE)
Protein
FYVE, RhoGEF and PH domain-containing protein 1
Gene
Fgd1
Organism
Mus musculus (Mouse)
Status
Functioni
Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 729 – 789 | FYVE-typePROSITE-ProRule annotationAdd BLAST | 61 |
GO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: MGI
- metal ion binding Source: UniProtKB-KW
- Rho guanyl-nucleotide exchange factor activity Source: GO_Central
- small GTPase binding Source: MGI
GO - Biological processi
- actin cytoskeleton organization Source: MGI
- cytoskeleton organization Source: MGI
- filopodium assembly Source: MGI
- regulation of cell shape Source: UniProtKB
- regulation of GTPase activity Source: MGI
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-193648, NRAGE signals death through JNK R-MMU-194840, Rho GTPase cycle R-MMU-416482, G alpha (12/13) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: FYVE, RhoGEF and PH domain-containing protein 1Alternative name(s): Faciogenital dysplasia 1 protein homolog Rho/Rac guanine nucleotide exchange factor FGD1 Short name: Rho/Rac GEF Zinc finger FYVE domain-containing protein 3 |
Gene namesi | Name:Fgd1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104566, Fgd1 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Other locations
- Cytoplasm 1 Publication
- lamellipodium 1 Publication
- ruffle 1 Publication
Note: Associated with membrane ruffles and lamellipodia.
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: MGI
Other locations
- cytoplasm Source: MGI
- lamellipodium Source: MGI
- ruffle Source: MGI
Keywords - Cellular componenti
Cell projection, Cytoplasm, CytoskeletonPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 159 | P → A: Abolishes binding to DBNL. 1 Publication | 1 | |
Mutagenesisi | 162 | P → A: Abolishes binding to DBNL. 1 Publication | 1 | |
Mutagenesisi | 164 | K → E: No effect on binding to DBNL. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080941 | 1 – 960 | FYVE, RhoGEF and PH domain-containing protein 1Add BLAST | 960 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 48 | PhosphoserineBy similarity | 1 | |
Modified residuei | 205 | PhosphoserineBy similarity | 1 | |
Modified residuei | 710 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 714 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P52734 |
MaxQBi | P52734 |
PaxDbi | P52734 |
PRIDEi | P52734 |
PTM databases
iPTMneti | P52734 |
PhosphoSitePlusi | P52734 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000025265, Expressed in rostral migratory stream and 273 other tissues |
ExpressionAtlasi | P52734, baseline and differential |
Genevisiblei | P52734, MM |
Interactioni
Subunit structurei
Interacts with DBNL/ABP1 and CTTN. Binds CDC42 (By similarity). May interact with CCPG1.
By similarity1 PublicationGO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: MGI
- Rho guanyl-nucleotide exchange factor activity Source: GO_Central
- small GTPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199637, 2 interactors |
IntActi | P52734, 2 interactors |
MINTi | P52734 |
STRINGi | 10090.ENSMUSP00000026296 |
Miscellaneous databases
RNActi | P52734, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 372 – 560 | DHPROSITE-ProRule annotationAdd BLAST | 189 | |
Domaini | 589 – 688 | PH 1PROSITE-ProRule annotationAdd BLAST | 100 | |
Domaini | 820 – 920 | PH 2PROSITE-ProRule annotationAdd BLAST | 101 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 171 – 187 | SH3-bindingSequence analysisAdd BLAST | 17 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 7 – 330 | Pro-richAdd BLAST | 324 |
Domaini
The DH domain is involved in interaction with CCPG1.
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 729 – 789 | FYVE-typePROSITE-ProRule annotationAdd BLAST | 61 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG4424, Eukaryota |
GeneTreei | ENSGT00940000159438 |
InParanoidi | P52734 |
OrthoDBi | 652460at2759 |
TreeFami | TF316247 |
Family and domain databases
CDDi | cd01219, PH1_FGD1, 1 hit cd13236, PH2_FGD1-4, 1 hit cd00160, RhoGEF, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 2 hits 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR035899, DBL_dom_sf IPR000219, DH-domain IPR035941, FGD1-4_PH2 IPR035939, FGD1_PH1 IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR000306, Znf_FYVE IPR017455, Znf_FYVE-rel IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF01363, FYVE, 1 hit PF00169, PH, 2 hits PF00621, RhoGEF, 1 hit |
SMARTi | View protein in SMART SM00064, FYVE, 1 hit SM00233, PH, 2 hits SM00325, RhoGEF, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit |
PROSITEi | View protein in PROSITE PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 2 hits PS50178, ZF_FYVE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P52734-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHGHRVPGGP GPSDPERSAA NTPGAAPLAC ADSDPGALEP GLPVSRGSGT
60 70 80 90 100
ALGGPLDPQF VGPSDASLGA PPSSRVLPCG PSPQHHRALR FSYHLEGSQP
110 120 130 140 150
RPGLHQGNRI LVKSLSLDPG QSLEPHPEGP QRLRSDPGPP TEIPGPRPSP
160 170 180 190 200
LKRAPGPKPQ VPPKPSYLQM PRVLPPPEPI PPPPSRPLPA DPRVAKGLVP
210 220 230 240 250
RAEASTSSAA VSSLIEKFER EPVIVASDRP APGPCPVPPE PAMLPQPPPQ
260 270 280 290 300
PTGSQLPEGE ASRCLFLLAP GPRDGEKVPN RDSGIDSISS PSNSEETCFV
310 320 330 340 350
SDDGPPIHSL CPGPPALASM PVALADPHRP GSQEVDSDLE EEEEEEEEEK
360 370 380 390 400
EREIPVPPME RQESVELTVQ QKVFHIANEL LQTEKAYVSR LHLLDQVFCA
410 420 430 440 450
RLLEEARNRS SFPADVVHGI FSNICSIYCF HQQFLLPELE KRMEEWDRYP
460 470 480 490 500
RIGDILQKLA PFLKMYGEYV KNFDRAVELV NTWTERSTQF KVIIHEVQKE
510 520 530 540 550
EACGNLTLQH HMLEPVQRIP RYELLLKDYL LKLPHGSPDS KDAQKSLELI
560 570 580 590 600
ATAAEHSNAA IRKMERMHKL LKVYELLGGE EDIVSPTKEL IKEGHILKLS
610 620 630 640 650
AKNGTTQDRY LILFNDRLLY CVPRLRLLGQ KFSVRARIDV DGMELKESSN
660 670 680 690 700
LNMPRTFLVS GKQRSLELQA RTEEEKKDWV QAINSTLLKH EQTLETFKLL
710 720 730 740 750
NSTNRDDEDT PPNSPNVDLG KRAPTPIREK EVTMCMRCQE PFNSITKRRH
760 770 780 790 800
HCKACGHVVC GKCSEFRARL IYDNNRSNRV CTDCYVALHG APGSSPACSQ
810 820 830 840 850
HTPQRRRSIL EKQASVAAEN SVICSFLHYM EKGGKGWHKA WFVVPENEPL
860 870 880 890 900
VLYIYGAPQD VKAQRSLPLI GFEVGPPEAG ERPDRRHVFK ITQSHLSWYF
910 920 930 940 950
SPETEELQRR WMAVLGRAGR GDTFCPGPTL SEDKEMEETP VAASGATAEP
960
PEASQTRDKT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA2ALP5 | A2ALP5_MOUSE | FYVE, RhoGEF and PH domain-containi... | Fgd1 | 791 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 504 | G → R in AAA96001 (PubMed:8535076).Curated | 1 | |
Sequence conflicti | 633 | S → T in AAA96001 (PubMed:8535076).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U22325 mRNA Translation: AAA96001.1 AL805937 Genomic DNA No translation available. BC011462 mRNA Translation: AAH11462.1 |
CCDSi | CCDS30467.1 |
RefSeqi | NP_032027.2, NM_008001.4 |
Genome annotation databases
Ensembli | ENSMUST00000026296; ENSMUSP00000026296; ENSMUSG00000025265 |
GeneIDi | 14163 |
KEGGi | mmu:14163 |
UCSCi | uc009uow.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U22325 mRNA Translation: AAA96001.1 AL805937 Genomic DNA No translation available. BC011462 mRNA Translation: AAH11462.1 |
CCDSi | CCDS30467.1 |
RefSeqi | NP_032027.2, NM_008001.4 |
3D structure databases
SMRi | P52734 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199637, 2 interactors |
IntActi | P52734, 2 interactors |
MINTi | P52734 |
STRINGi | 10090.ENSMUSP00000026296 |
PTM databases
iPTMneti | P52734 |
PhosphoSitePlusi | P52734 |
Proteomic databases
jPOSTi | P52734 |
MaxQBi | P52734 |
PaxDbi | P52734 |
PRIDEi | P52734 |
Protocols and materials databases
Antibodypediai | 376, 59 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000026296; ENSMUSP00000026296; ENSMUSG00000025265 |
GeneIDi | 14163 |
KEGGi | mmu:14163 |
UCSCi | uc009uow.2, mouse |
Organism-specific databases
CTDi | 2245 |
MGIi | MGI:104566, Fgd1 |
Phylogenomic databases
eggNOGi | KOG4424, Eukaryota |
GeneTreei | ENSGT00940000159438 |
InParanoidi | P52734 |
OrthoDBi | 652460at2759 |
TreeFami | TF316247 |
Enzyme and pathway databases
Reactomei | R-MMU-193648, NRAGE signals death through JNK R-MMU-194840, Rho GTPase cycle R-MMU-416482, G alpha (12/13) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 14163, 5 hits in 17 CRISPR screens |
ChiTaRSi | Fgd1, mouse |
PROi | PR:P52734 |
RNActi | P52734, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025265, Expressed in rostral migratory stream and 273 other tissues |
ExpressionAtlasi | P52734, baseline and differential |
Genevisiblei | P52734, MM |
Family and domain databases
CDDi | cd01219, PH1_FGD1, 1 hit cd13236, PH2_FGD1-4, 1 hit cd00160, RhoGEF, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 2 hits 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR035899, DBL_dom_sf IPR000219, DH-domain IPR035941, FGD1-4_PH2 IPR035939, FGD1_PH1 IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR000306, Znf_FYVE IPR017455, Znf_FYVE-rel IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF01363, FYVE, 1 hit PF00169, PH, 2 hits PF00621, RhoGEF, 1 hit |
SMARTi | View protein in SMART SM00064, FYVE, 1 hit SM00233, PH, 2 hits SM00325, RhoGEF, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit |
PROSITEi | View protein in PROSITE PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 2 hits PS50178, ZF_FYVE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FGD1_MOUSE | |
Accessioni | P52734Primary (citable) accession number: P52734 Secondary accession number(s): Q921L2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 167 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot