UniProtKB - P52655 (TF2AA_HUMAN)
Transcription initiation factor IIA subunit 1
GTF2A1
Functioni
GO - Molecular functioni
- DNA binding Source: BHF-UCL
- protein heterodimerization activity Source: BHF-UCL
- RNA polymerase II core promoter sequence-specific DNA binding Source: ARUK-UCL
- RNA polymerase II general transcription initiation factor activity Source: ARUK-UCL
- RNA polymerase II repressing transcription factor binding Source: Ensembl
- TBP-class protein binding Source: ARUK-UCL
- transcription factor binding Source: BHF-UCL
GO - Biological processi
- snRNA transcription by RNA polymerase II Source: Reactome
- transcription by RNA polymerase II Source: ARUK-UCL
- transcription initiation from RNA polymerase II promoter Source: Reactome
Keywordsi
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | P52655 |
Reactomei | R-HSA-167161, HIV Transcription Initiation R-HSA-167162, RNA Polymerase II HIV Promoter Escape R-HSA-167172, Transcription of the HIV genome R-HSA-674695, RNA Polymerase II Pre-transcription Events R-HSA-6807505, RNA polymerase II transcribes snRNA genes R-HSA-73776, RNA Polymerase II Promoter Escape R-HSA-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-HSA-75953, RNA Polymerase II Transcription Initiation R-HSA-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-HSA-9018519, Estrogen-dependent gene expression |
SIGNORi | P52655 |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription initiation factor IIA subunit 1Alternative name(s): General transcription factor IIA subunit 1 TFIIAL Transcription initiation factor TFIIA 42 kDa subunit Short name: TFIIA-42 Cleaved into the following 2 chains: Alternative name(s): TFIIA p35 subunit Alternative name(s): TFIIA p19 subunit |
Gene namesi | Name:GTF2A1 Synonyms:TF2A1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000165417.11 |
HGNCi | HGNC:4646, GTF2A1 |
MIMi | 600520, gene |
neXtProti | NX_P52655 |
Subcellular locationi
Nucleus
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
- transcription factor TFIIA complex Source: BHF-UCL
- transcription factor TFIID complex Source: UniProtKB
Other locations
- transcription preinitiation complex Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 270 | V → A: Slightly affects cleavage and yields elevated levels of the precursor. 1 Publication | 1 | |
Mutagenesisi | 272 | Q → A: Abolishes cleavage. 1 Publication | 1 | |
Mutagenesisi | 273 | V → A: Abolishes cleavage. 1 Publication | 1 | |
Mutagenesisi | 274 | D → A: Abolishes cleavage. 1 Publication | 1 | |
Mutagenesisi | 275 | G → A: Abolishes cleavage. 1 Publication | 1 | |
Mutagenesisi | 276 | T → A: Does not affect cleavage. 1 Publication | 1 | |
Mutagenesisi | 277 | G → A: Does not affect cleavage. 1 Publication | 1 | |
Mutagenesisi | 278 | D → A: Significant reduction of cleavage. 1 Publication | 1 | |
Mutagenesisi | 280 | S → A: Slightly affects cleavage, yields elevated levels of the precursor. Eliminates phosphorylation; when associated with A-281; A-316 and A-321. 2 Publications | 1 | |
Mutagenesisi | 281 | S → A: Eliminates phosphorylation; when associated with A-280; A-316 and A-321. 1 Publication | 1 | |
Mutagenesisi | 282 | E → A: Slightly affects cleavage and yields elevated levels of the precursor. 1 Publication | 1 | |
Mutagenesisi | 316 | S → A: Strongly reduces phosphorylation; when associated with A-321. Eliminates phosphorylation; when associated with A-280; A-281 and A-321. 1 Publication | 1 | |
Mutagenesisi | 321 | S → A: Strongly reduces phosphorylation; when associated with A-316. Eliminates phosphorylation; when associated with A-280; A-281 and A-316. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 2957 |
OpenTargetsi | ENSG00000165417 |
PharmGKBi | PA29033 |
Miscellaneous databases
Pharosi | P52655, Tchem |
Chemistry databases
ChEMBLi | CHEMBL4832 |
Polymorphism and mutation databases
BioMutai | GTF2A1 |
DMDMi | 1711663 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000022481 | 2 – 376 | Transcription initiation factor IIA subunit 1Add BLAST | 375 | |
ChainiPRO_0000042593 | 2 – 274 | Transcription initiation factor IIA alpha chainAdd BLAST | 273 | |
ChainiPRO_0000042594 | 275 – 376 | Transcription initiation factor IIA beta chainAdd BLAST | 102 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 280 | Phosphoserine; by TAF11 Publication | 1 | |
Modified residuei | 281 | Phosphoserine; by TAF11 Publication | 1 | |
Modified residuei | 316 | Phosphoserine; by TAF1Combined sources1 Publication | 1 | |
Modified residuei | 321 | Phosphoserine; by TAF1Combined sources1 Publication | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 274 – 275 | Cleavage; by TASP1 | 2 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P52655 |
jPOSTi | P52655 |
MassIVEi | P52655 |
MaxQBi | P52655 |
PaxDbi | P52655 |
PeptideAtlasi | P52655 |
PRIDEi | P52655 |
ProteomicsDBi | 56499 [P52655-1] 56500 [P52655-2] |
PTM databases
GlyGeni | P52655, 3 sites, 1 O-linked glycan (3 sites) |
iPTMneti | P52655 |
PhosphoSitePlusi | P52655 |
Expressioni
Gene expression databases
Bgeei | ENSG00000165417, Expressed in forebrain and 220 other tissues |
ExpressionAtlasi | P52655, baseline and differential |
Genevisiblei | P52655, HS |
Organism-specific databases
HPAi | ENSG00000165417, Low tissue specificity |
Interactioni
Subunit structurei
TFIIA is a heterodimer of the large unprocessed subunit 1 and a small subunit gamma. It was originally believed to be a heterotrimer of an alpha (p35), a beta (p19) and a gamma subunit (p12). TFIIA forms a complex with TBP.
1 PublicationBinary interactionsi
Hide detailsP52655
GO - Molecular functioni
- protein heterodimerization activity Source: BHF-UCL
- RNA polymerase II repressing transcription factor binding Source: Ensembl
- TBP-class protein binding Source: ARUK-UCL
- transcription factor binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 109212, 66 interactors |
ComplexPortali | CPX-519, Transcription factor complex TFIIA |
CORUMi | P52655 |
DIPi | DIP-33225N |
IntActi | P52655, 18 interactors |
MINTi | P52655 |
STRINGi | 9606.ENSP00000452454 |
Chemistry databases
BindingDBi | P52655 |
Miscellaneous databases
RNActi | P52655, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P52655 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P52655 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 71 – 80 | Poly-Gln | 10 | |
Compositional biasi | 81 – 87 | Poly-His | 7 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2652, Eukaryota |
GeneTreei | ENSGT00940000156726 |
HOGENOMi | CLU_030027_5_0_1 |
InParanoidi | P52655 |
OMAi | MLCTYDK |
OrthoDBi | 1443035at2759 |
PhylomeDBi | P52655 |
TreeFami | TF350445 |
Family and domain databases
Gene3Di | 2.30.18.10, 1 hit |
IDEALi | IID00555 |
InterProi | View protein in InterPro IPR004855, TFIIA_asu/bsu IPR009088, TFIIA_b-brl |
PANTHERi | PTHR12694, PTHR12694, 2 hits |
Pfami | View protein in Pfam PF03153, TFIIA, 2 hits |
SMARTi | View protein in SMART SM01371, TFIIA, 1 hit |
SUPFAMi | SSF50784, SSF50784, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MANSANTNTV PKLYRSVIED VINDVRDIFL DDGVDEQVLM ELKTLWENKL
60 70 80 90 100
MQSRAVDGFH SEEQQLLLQV QQQHQPQQQQ HHHHHHHQQA QPQQTVPQQA
110 120 130 140 150
QTQQVLIPAS QQATAPQVIV PDSKLIQHMN ASNMSAAATA ATLALPAGVT
160 170 180 190 200
PVQQILTNSG QLLQVVRAAN GAQYIFQPQQ SVVLQQQVIP QMQPGGVQAP
210 220 230 240 250
VIQQVLAPLP GGISPQTGVI IQPQQILFTG NKTQVIPTTV AAPTPAQAQI
260 270 280 290 300
TATGQQQPQA QPAQTQAPLV LQVDGTGDTS SEEDEDEEED YDDDEEEDKE
310 320 330 340 350
KDGAEDGQVE EEPLNSEDDV SDEEGQELFD TENVVVCQYD KIHRSKNKWK
360 370
FHLKDGIMNL NGRDYIFSKA IGDAEW
The sequence of this isoform differs from the canonical sequence as follows:
1-39: Missing.
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketJ3KNC0 | J3KNC0_HUMAN | Transcription initiation factor IIA... | GTF2A1 | 59 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_035667 | 30 | L → V in a breast cancer sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_054043 | 109 | A → P. Corresponds to variant dbSNP:rs17111579Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018798 | 1 – 39 | Missing in isoform 37 kDa. CuratedAdd BLAST | 39 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X75383 mRNA Translation: CAA53151.1 X75383 mRNA Translation: CAA53152.1 X77225 mRNA Translation: CAA54442.1 D14887 mRNA Translation: BAA03604.1 D14886 mRNA Translation: BAA03603.1 AC010582 Genomic DNA Translation: AAF26776.1 BC107155 mRNA Translation: AAI07156.1 BC107156 mRNA Translation: AAI07157.1 |
CCDSi | CCDS9873.1 [P52655-1] CCDS9874.1 [P52655-2] |
PIRi | A49077 |
RefSeqi | NP_001265869.1, NM_001278940.1 NP_056943.1, NM_015859.3 [P52655-1] NP_963889.1, NM_201595.2 [P52655-2] |
Genome annotation databases
Ensembli | ENST00000434192; ENSP00000409492; ENSG00000165417 [P52655-2] ENST00000553612; ENSP00000452454; ENSG00000165417 [P52655-1] |
GeneIDi | 2957 |
KEGGi | hsa:2957 |
UCSCi | uc001xvf.4, human [P52655-1] |
Keywords - Coding sequence diversityi
Alternative initiation, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X75383 mRNA Translation: CAA53151.1 X75383 mRNA Translation: CAA53152.1 X77225 mRNA Translation: CAA54442.1 D14887 mRNA Translation: BAA03604.1 D14886 mRNA Translation: BAA03603.1 AC010582 Genomic DNA Translation: AAF26776.1 BC107155 mRNA Translation: AAI07156.1 BC107156 mRNA Translation: AAI07157.1 |
CCDSi | CCDS9873.1 [P52655-1] CCDS9874.1 [P52655-2] |
PIRi | A49077 |
RefSeqi | NP_001265869.1, NM_001278940.1 NP_056943.1, NM_015859.3 [P52655-1] NP_963889.1, NM_201595.2 [P52655-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NVP | X-ray | 2.10 | B | 2-58 | [»] | |
C | 303-376 | [»] | ||||
5FUR | electron microscopy | 8.50 | B | 9-51 | [»] | |
C | 330-376 | [»] | ||||
5IY6 | electron microscopy | 7.20 | N | 1-376 | [»] | |
5IY7 | electron microscopy | 8.60 | N | 1-376 | [»] | |
5IY8 | electron microscopy | 7.90 | N | 1-376 | [»] | |
5IY9 | electron microscopy | 6.30 | N | 1-376 | [»] | |
5IYA | electron microscopy | 5.40 | N | 1-376 | [»] | |
5IYB | electron microscopy | 3.90 | N | 1-376 | [»] | |
5IYC | electron microscopy | 3.90 | N | 1-376 | [»] | |
5IYD | electron microscopy | 3.90 | N | 1-376 | [»] | |
5M4S | X-ray | 2.38 | A | 2-58 | [»] | |
A | 328-376 | [»] | ||||
6MZM | electron microscopy | 7.50 | W | 9-376 | [»] | |
6O9L | electron microscopy | 7.20 | N | 1-376 | [»] | |
SMRi | P52655 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 109212, 66 interactors |
ComplexPortali | CPX-519, Transcription factor complex TFIIA |
CORUMi | P52655 |
DIPi | DIP-33225N |
IntActi | P52655, 18 interactors |
MINTi | P52655 |
STRINGi | 9606.ENSP00000452454 |
Chemistry databases
BindingDBi | P52655 |
ChEMBLi | CHEMBL4832 |
PTM databases
GlyGeni | P52655, 3 sites, 1 O-linked glycan (3 sites) |
iPTMneti | P52655 |
PhosphoSitePlusi | P52655 |
Polymorphism and mutation databases
BioMutai | GTF2A1 |
DMDMi | 1711663 |
Proteomic databases
EPDi | P52655 |
jPOSTi | P52655 |
MassIVEi | P52655 |
MaxQBi | P52655 |
PaxDbi | P52655 |
PeptideAtlasi | P52655 |
PRIDEi | P52655 |
ProteomicsDBi | 56499 [P52655-1] 56500 [P52655-2] |
Protocols and materials databases
Antibodypediai | 76, 298 antibodies |
DNASUi | 2957 |
Genome annotation databases
Ensembli | ENST00000434192; ENSP00000409492; ENSG00000165417 [P52655-2] ENST00000553612; ENSP00000452454; ENSG00000165417 [P52655-1] |
GeneIDi | 2957 |
KEGGi | hsa:2957 |
UCSCi | uc001xvf.4, human [P52655-1] |
Organism-specific databases
CTDi | 2957 |
DisGeNETi | 2957 |
EuPathDBi | HostDB:ENSG00000165417.11 |
GeneCardsi | GTF2A1 |
HGNCi | HGNC:4646, GTF2A1 |
HPAi | ENSG00000165417, Low tissue specificity |
MIMi | 600520, gene |
neXtProti | NX_P52655 |
OpenTargetsi | ENSG00000165417 |
PharmGKBi | PA29033 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2652, Eukaryota |
GeneTreei | ENSGT00940000156726 |
HOGENOMi | CLU_030027_5_0_1 |
InParanoidi | P52655 |
OMAi | MLCTYDK |
OrthoDBi | 1443035at2759 |
PhylomeDBi | P52655 |
TreeFami | TF350445 |
Enzyme and pathway databases
PathwayCommonsi | P52655 |
Reactomei | R-HSA-167161, HIV Transcription Initiation R-HSA-167162, RNA Polymerase II HIV Promoter Escape R-HSA-167172, Transcription of the HIV genome R-HSA-674695, RNA Polymerase II Pre-transcription Events R-HSA-6807505, RNA polymerase II transcribes snRNA genes R-HSA-73776, RNA Polymerase II Promoter Escape R-HSA-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-HSA-75953, RNA Polymerase II Transcription Initiation R-HSA-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-HSA-9018519, Estrogen-dependent gene expression |
SIGNORi | P52655 |
Miscellaneous databases
BioGRID-ORCSi | 2957, 607 hits in 850 CRISPR screens |
ChiTaRSi | GTF2A1, human |
EvolutionaryTracei | P52655 |
GeneWikii | GTF2A1 |
GenomeRNAii | 2957 |
Pharosi | P52655, Tchem |
PROi | PR:P52655 |
RNActi | P52655, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000165417, Expressed in forebrain and 220 other tissues |
ExpressionAtlasi | P52655, baseline and differential |
Genevisiblei | P52655, HS |
Family and domain databases
Gene3Di | 2.30.18.10, 1 hit |
IDEALi | IID00555 |
InterProi | View protein in InterPro IPR004855, TFIIA_asu/bsu IPR009088, TFIIA_b-brl |
PANTHERi | PTHR12694, PTHR12694, 2 hits |
Pfami | View protein in Pfam PF03153, TFIIA, 2 hits |
SMARTi | View protein in SMART SM01371, TFIIA, 1 hit |
SUPFAMi | SSF50784, SSF50784, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TF2AA_HUMAN | |
Accessioni | P52655Primary (citable) accession number: P52655 Secondary accession number(s): Q3KNQ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 202 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations