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UniProtKB - P52647 (NIFJ_ECOLI)
Protein
Probable pyruvate-flavodoxin oxidoreductase
Gene
ydbK
Organism
Escherichia coli (strain K12)
Status
Functioni
Oxidoreductase required for the transfer of electrons from pyruvate to flavodoxin.
CuratedCatalytic activityi
Cofactori
[4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 689 | Iron-sulfur (4Fe-4S) 1By similarity | 1 | |
Metal bindingi | 692 | Iron-sulfur (4Fe-4S) 1By similarity | 1 | |
Metal bindingi | 695 | Iron-sulfur (4Fe-4S) 1By similarity | 1 | |
Metal bindingi | 699 | Iron-sulfur (4Fe-4S) 2By similarity | 1 | |
Metal bindingi | 745 | Iron-sulfur (4Fe-4S) 2By similarity | 1 | |
Metal bindingi | 748 | Iron-sulfur (4Fe-4S) 2By similarity | 1 | |
Metal bindingi | 751 | Iron-sulfur (4Fe-4S) 2By similarity | 1 | |
Metal bindingi | 755 | Iron-sulfur (4Fe-4S) 1By similarity | 1 | |
Metal bindingi | 819 | Iron-sulfur (4Fe-4S) 3By similarity | 1 | |
Metal bindingi | 822 | Iron-sulfur (4Fe-4S) 3By similarity | 1 | |
Metal bindingi | 847 | Iron-sulfur (4Fe-4S) 3By similarity | 1 | |
Metal bindingi | 1071 | Iron-sulfur (4Fe-4S) 3By similarity | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- iron ion binding Source: InterPro
- pyruvate-flavodoxin oxidoreductase activity Source: EcoCyc
- thiamine pyrophosphate binding Source: InterPro
GO - Biological processi
- electron transport chain Source: InterPro
- response to oxidative stress Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Transport |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G6701-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ydbK Ordered Locus Names:b1378, JW1372 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215557 | 1 – 1174 | Probable pyruvate-flavodoxin oxidoreductaseAdd BLAST | 1174 |
Proteomic databases
jPOSTi | P52647 |
PaxDbi | P52647 |
PRIDEi | P52647 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261676, 23 interactors |
DIPi | DIP-11636N |
IntActi | P52647, 10 interactors |
STRINGi | 511145.b1378 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 680 – 709 | 4Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST | 30 | |
Domaini | 736 – 765 | 4Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST | 30 |
Sequence similaritiesi
Belongs to the pyruvate:ferredoxin/flavodoxin oxidoreductase family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG0674, Bacteria COG1013, Bacteria COG1014, Bacteria COG1143, Bacteria |
HOGENOMi | CLU_002569_0_0_6 |
InParanoidi | P52647 |
OMAi | NTVMQVC |
PhylomeDBi | P52647 |
Family and domain databases
CDDi | cd07034, TPP_PYR_PFOR_IOR-alpha_like, 1 hit |
Gene3Di | 3.40.50.920, 1 hit 3.40.920.10, 1 hit 4.10.780.10, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR033412, PFOR_II IPR037112, Pyrv-flavodox_OxR_EKR_sf IPR019456, Pyrv-flavodox_OxRtase_EKR IPR019752, Pyrv/ketoisovalerate_OxRed_cat IPR002880, Pyrv_Fd/Flavodoxin_OxRdtase_N IPR011895, Pyrv_flavodox_OxRed IPR002869, Pyrv_flavodox_OxRed_cen IPR029061, THDP-binding IPR011766, TPP_enzyme-bd_C IPR009014, Transketo_C/PFOR_II |
Pfami | View protein in Pfam PF10371, EKR, 1 hit PF17147, PFOR_II, 1 hit PF01558, POR, 1 hit PF01855, POR_N, 1 hit PF02775, TPP_enzyme_C, 1 hit |
PIRSFi | PIRSF000159, NifJ, 1 hit |
SMARTi | View protein in SMART SM00890, EKR, 1 hit |
SUPFAMi | SSF52518, SSF52518, 2 hits SSF52922, SSF52922, 1 hit SSF53323, SSF53323, 1 hit |
TIGRFAMsi | TIGR02176, pyruv_ox_red, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 2 hits PS51379, 4FE4S_FER_2, 2 hits |
i Sequence
Sequence statusi: Complete.
P52647-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MITIDGNGAV ASVAFRTSEV IAIYPITPSS TMAEQADAWA GNGLKNVWGD
60 70 80 90 100
TPRVVEMQSE AGAIATVHGA LQTGALSTSF TSSQGLLLMI PTLYKLAGEL
110 120 130 140 150
TPFVLHVAAR TVATHALSIF GDHSDVMAVR QTGCAMLCAA NVQEAQDFAL
160 170 180 190 200
ISQIATLKSR VPFIHFFDGF RTSHEINKIV PLADDTILDL MPQVEIDAHR
210 220 230 240 250
ARALNPEHPV IRGTSANPDT YFQSREATNP WYNAVYDHVE QAMNDFSAAT
260 270 280 290 300
GRQYQPFEYY GHPQAERVII LMGSAIGTCE EVVDELLTRG EKVGVLKVRL
310 320 330 340 350
YRPFSAKHLL QALPGSVRSV AVLDRTKEPG AQAEPLYLDV MTALAEAFNN
360 370 380 390 400
GERETLPRVI GGRYGLSSKE FGPDCVLAVF AELNAAKPKA RFTVGIYDDV
410 420 430 440 450
TNLSLPLPEN TLPNSAKLEA LFYGLGSDGS VSATKNNIKI IGNSTPWYAQ
460 470 480 490 500
GYFVYDSKKA GGLTVSHLRV SEQPIRSAYL ISQADFVGCH QLQFIDKYQM
510 520 530 540 550
AERLKPGGIF LLNTPYSADE VWSRLPQEVQ AVLNQKKARF YVINAAKIAR
560 570 580 590 600
ECGLAARINT VMQMAFFHLT QILPGDSALA ELQGAIAKSY SSKGQDLVER
610 620 630 640 650
NWQALALARE SVEEVPLQPV NPHSANRPPV VSDAAPDFVK TVTAAMLAGL
660 670 680 690 700
GDALPVSALP PDGTWPMGTT RWEKRNIAEE IPIWKEELCT QCNHCVAACP
710 720 730 740 750
HSAIRAKVVP PEAMENAPAS LHSLDVKSRD MRGQKYVLQV APEDCTGCNL
760 770 780 790 800
CVEVCPAKDR QNPEIKAINM MSRLEHVEEE KINYDFFLNL PEIDRSKLER
810 820 830 840 850
IDIRTSQLIT PLFEYSGACS GCGETPYIKL LTQLYGDRML IANATGCSSI
860 870 880 890 900
YGGNLPSTPY TTDANGRGPA WANSLFEDNA EFGLGFRLTV DQHRVRVLRL
910 920 930 940 950
LDQFADKIPA ELLTALKSDA TPEVRREQVA ALRQQLNDVA EAHELLRDAD
960 970 980 990 1000
ALVEKSIWLI GGDGWAYDIG FGGLDHVLSL TENVNILVLD TQCYSNTGGQ
1010 1020 1030 1040 1050
ASKATPLGAV TKFGEHGKRK ARKDLGVSMM MYGHVYVAQI SLGAQLNQTV
1060 1070 1080 1090 1100
KAIQEAEAYP GPSLIIAYSP CEEHGYDLAL SHDQMRQLTA TGFWPLYRFD
1110 1120 1130 1140 1150
PRRADEGKLP LALDSRPPSE APEETLLHEQ RFRRLNSQQP EVAEQLWKDA
1160 1170
AADLQKRYDF LAQMAGKAEK SNTD
Sequence cautioni
The sequence U36928 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 40 | A → R (PubMed:9025293).Curated | 1 | |
Sequence conflicti | 51 | T → S (PubMed:9025293).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74460.1 AP009048 Genomic DNA Translation: BAA14982.1 U36928 Genomic DNA No translation available. |
PIRi | E64888 |
RefSeqi | NP_415896.1, NC_000913.3 WP_000628244.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC74460; AAC74460; b1378 BAA14982; BAA14982; BAA14982 |
GeneIDi | 946587 |
KEGGi | ecj:JW1372 eco:b1378 |
PATRICi | fig|511145.12.peg.1440 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74460.1 AP009048 Genomic DNA Translation: BAA14982.1 U36928 Genomic DNA No translation available. |
PIRi | E64888 |
RefSeqi | NP_415896.1, NC_000913.3 WP_000628244.1, NZ_LN832404.1 |
3D structure databases
AlphaFoldDBi | P52647 |
SMRi | P52647 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261676, 23 interactors |
DIPi | DIP-11636N |
IntActi | P52647, 10 interactors |
STRINGi | 511145.b1378 |
Chemistry databases
DrugBanki | DB00698, Nitrofurantoin |
Proteomic databases
jPOSTi | P52647 |
PaxDbi | P52647 |
PRIDEi | P52647 |
Genome annotation databases
EnsemblBacteriai | AAC74460; AAC74460; b1378 BAA14982; BAA14982; BAA14982 |
GeneIDi | 946587 |
KEGGi | ecj:JW1372 eco:b1378 |
PATRICi | fig|511145.12.peg.1440 |
Organism-specific databases
EchoBASEi | EB2975 |
Phylogenomic databases
eggNOGi | COG0674, Bacteria COG1013, Bacteria COG1014, Bacteria COG1143, Bacteria |
HOGENOMi | CLU_002569_0_0_6 |
InParanoidi | P52647 |
OMAi | NTVMQVC |
PhylomeDBi | P52647 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6701-MONOMER |
Miscellaneous databases
PROi | PR:P52647 |
Family and domain databases
CDDi | cd07034, TPP_PYR_PFOR_IOR-alpha_like, 1 hit |
Gene3Di | 3.40.50.920, 1 hit 3.40.920.10, 1 hit 4.10.780.10, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR033412, PFOR_II IPR037112, Pyrv-flavodox_OxR_EKR_sf IPR019456, Pyrv-flavodox_OxRtase_EKR IPR019752, Pyrv/ketoisovalerate_OxRed_cat IPR002880, Pyrv_Fd/Flavodoxin_OxRdtase_N IPR011895, Pyrv_flavodox_OxRed IPR002869, Pyrv_flavodox_OxRed_cen IPR029061, THDP-binding IPR011766, TPP_enzyme-bd_C IPR009014, Transketo_C/PFOR_II |
Pfami | View protein in Pfam PF10371, EKR, 1 hit PF17147, PFOR_II, 1 hit PF01558, POR, 1 hit PF01855, POR_N, 1 hit PF02775, TPP_enzyme_C, 1 hit |
PIRSFi | PIRSF000159, NifJ, 1 hit |
SMARTi | View protein in SMART SM00890, EKR, 1 hit |
SUPFAMi | SSF52518, SSF52518, 2 hits SSF52922, SSF52922, 1 hit SSF53323, SSF53323, 1 hit |
TIGRFAMsi | TIGR02176, pyruv_ox_red, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 2 hits PS51379, 4FE4S_FER_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | NIFJ_ECOLI | |
Accessioni | P52647Primary (citable) accession number: P52647 Secondary accession number(s): P77238 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 176 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families