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UniProtKB - P52643 (LDHD_ECOLI)
Protein
D-lactate dehydrogenase
Gene
ldhA
Organism
Escherichia coli (strain K12)
Status
Functioni
Fermentative lactate dehydrogenase.
Catalytic activityi
- EC:1.1.1.28
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 174 | NADBy similarity | 1 | |
Binding sitei | 211 | NADBy similarity | 1 | |
Active sitei | 234 | By similarity | 1 | |
Binding sitei | 258 | NADBy similarity | 1 | |
Active sitei | 263 | By similarity | 1 | |
Active sitei | 295 | Proton donorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 154 – 155 | NADBy similarity | 2 | |
Nucleotide bindingi | 205 – 206 | NADBy similarity | 2 | |
Nucleotide bindingi | 232 – 234 | NADBy similarity | 3 |
GO - Molecular functioni
- D-lactate dehydrogenase activity Source: EcoCyc
- NADH binding Source: EcoliWiki
GO - Biological processi
- mixed acid fermentation Source: EcoCyc
- response to heat Source: EcoliWiki
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Stress response |
Ligand | NAD |
Enzyme and pathway databases
BioCyci | EcoCyc:DLACTDEHYDROGNAD-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: D-lactate dehydrogenase (EC:1.1.1.28)Short name: D-LDH Alternative name(s): Fermentative lactate dehydrogenase |
Gene namesi | Name:ldhA Synonyms:hslI, htpH Ordered Locus Names:b1380, JW1375 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000075952 | 1 – 329 | D-lactate dehydrogenaseAdd BLAST | 329 |
Proteomic databases
jPOSTi | P52643 |
PaxDbi | P52643 |
PRIDEi | P52643 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259502, 26 interactors |
DIPi | DIP-10087N |
IntActi | P52643, 4 interactors |
STRINGi | 511145.b1380 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P52643 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | COG1052, Bacteria |
HOGENOMi | CLU_019796_1_1_6 |
InParanoidi | P52643 |
PhylomeDBi | P52643 |
Family and domain databases
InterProi | View protein in InterPro IPR006139, D-isomer_2_OHA_DH_cat_dom IPR029753, D-isomer_DH_CS IPR029752, D-isomer_DH_CS1 IPR006140, D-isomer_DH_NAD-bd IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00389, 2-Hacid_dh, 1 hit PF02826, 2-Hacid_dh_C, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00065, D_2_HYDROXYACID_DH_1, 1 hit PS00670, D_2_HYDROXYACID_DH_2, 1 hit PS00671, D_2_HYDROXYACID_DH_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P52643-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLAVYSTKQ YDKKYLQQVN ESFGFELEFF DFLLTEKTAK TANGCEAVCI
60 70 80 90 100
FVNDDGSRPV LEELKKHGVK YIALRCAGFN NVDLDAAKEL GLKVVRVPAY
110 120 130 140 150
DPEAVAEHAI GMMMTLNRRI HRAYQRTRDA NFSLEGLTGF TMYGKTAGVI
160 170 180 190 200
GTGKIGVAML RILKGFGMRL LAFDPYPSAA ALELGVEYVD LPTLFSESDV
210 220 230 240 250
ISLHCPLTPE NYHLLNEAAF EQMKNGVMIV NTSRGALIDS QAAIEALKNQ
260 270 280 290 300
KIGSLGMDVY ENERDLFFED KSNDVIQDDV FRRLSACHNV LFTGHQAFLT
310 320
AEALTSISQT TLQNLSNLEK GETCPNELV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U36928 Genomic DNA Translation: AAB51772.1 U00096 Genomic DNA Translation: AAC74462.1 AP009048 Genomic DNA Translation: BAA14990.1 |
PIRi | G64888 |
RefSeqi | NP_415898.1, NC_000913.3 WP_000762236.1, NZ_SSZK01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC74462; AAC74462; b1380 BAA14990; BAA14990; BAA14990 |
GeneIDi | 946315 |
KEGGi | ecj:JW1375 eco:b1380 |
PATRICi | fig|1411691.4.peg.892 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U36928 Genomic DNA Translation: AAB51772.1 U00096 Genomic DNA Translation: AAC74462.1 AP009048 Genomic DNA Translation: BAA14990.1 |
PIRi | G64888 |
RefSeqi | NP_415898.1, NC_000913.3 WP_000762236.1, NZ_SSZK01000012.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5Z1Z | X-ray | 1.97 | A/B/C/D | 1-329 | [»] | |
SMRi | P52643 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259502, 26 interactors |
DIPi | DIP-10087N |
IntActi | P52643, 4 interactors |
STRINGi | 511145.b1380 |
Proteomic databases
jPOSTi | P52643 |
PaxDbi | P52643 |
PRIDEi | P52643 |
Genome annotation databases
EnsemblBacteriai | AAC74462; AAC74462; b1380 BAA14990; BAA14990; BAA14990 |
GeneIDi | 946315 |
KEGGi | ecj:JW1375 eco:b1380 |
PATRICi | fig|1411691.4.peg.892 |
Organism-specific databases
EchoBASEi | EB2978 |
Phylogenomic databases
eggNOGi | COG1052, Bacteria |
HOGENOMi | CLU_019796_1_1_6 |
InParanoidi | P52643 |
PhylomeDBi | P52643 |
Enzyme and pathway databases
BioCyci | EcoCyc:DLACTDEHYDROGNAD-MONOMER |
Miscellaneous databases
PROi | PR:P52643 |
Family and domain databases
InterProi | View protein in InterPro IPR006139, D-isomer_2_OHA_DH_cat_dom IPR029753, D-isomer_DH_CS IPR029752, D-isomer_DH_CS1 IPR006140, D-isomer_DH_NAD-bd IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00389, 2-Hacid_dh, 1 hit PF02826, 2-Hacid_dh_C, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00065, D_2_HYDROXYACID_DH_1, 1 hit PS00670, D_2_HYDROXYACID_DH_2, 1 hit PS00671, D_2_HYDROXYACID_DH_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LDHD_ECOLI | |
Accessioni | P52643Primary (citable) accession number: P52643 Secondary accession number(s): P78152 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families