UniProtKB - P52624 (UPP1_MOUSE)
Protein
Uridine phosphorylase 1
Gene
Upp1
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate (PubMed:7744869). The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.Curated1 Publication
Catalytic activityi
- EC:2.4.2.31 Publication
Activity regulationi
Strongly inhibited by 2,2'-anhydro-5-ethyluridine, a competitive inhibitor.1 Publication
Kineticsi
- KM=64 µM for uridine1 Publication
- KM=148 µM for thymidine1 Publication
: UMP biosynthesis via salvage pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes uracil from uridine (phosphorylase route).CuratedProteins known to be involved in this subpathway in this organism are:
- Uridine phosphorylase (Upp2), Uridine phosphorylase (Upp1), Uridine phosphorylase (Upp2), Uridine phosphorylase (Upp2), Uridine phosphorylase (Upp2), Uridine phosphorylase (Upp2), Uridine phosphorylase 2 (Upp2), Uridine phosphorylase (Upp2), Uridine phosphorylase 1 (Upp1), Uridine phosphorylase (Upp2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes uracil from uridine (phosphorylase route), the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 61 | Phosphate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 95 | PhosphateBy similarity | 1 |
GO - Molecular functioni
- identical protein binding Source: UniProtKB
- uridine phosphorylase activity Source: UniProtKB
GO - Biological processi
- cellular response to glucose starvation Source: Ensembl
- nucleotide catabolic process Source: InterPro
- pyrimidine nucleotide metabolic process Source: MGI
- UMP salvage Source: UniProtKB-UniPathway
- uridine catabolic process Source: UniProtKB
- uridine metabolic process Source: MGI
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
Reactomei | R-MMU-73614, Pyrimidine salvage R-MMU-73621, Pyrimidine catabolism |
UniPathwayi | UPA00574;UER00633 |
Names & Taxonomyi
Protein namesi | Recommended name: Uridine phosphorylase 1 (EC:2.4.2.31 Publication)Short name: UPase 1 Short name: UrdPase 1 |
Gene namesi | Name:Upp1 Synonyms:Up, Upp |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1097668, Upp1 |
Subcellular locationi
Nucleus
- nucleoplasm Source: MGI
Other locations
- cytoplasm Source: InterPro
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063192 | 1 – 311 | Uridine phosphorylase 1Add BLAST | 311 |
Post-translational modificationi
The N-terminus is blocked.
Proteomic databases
jPOSTi | P52624 |
PaxDbi | P52624 |
PeptideAtlasi | P52624 |
PRIDEi | P52624 |
PTM databases
PhosphoSitePlusi | P52624 |
SwissPalmi | P52624 |
Expressioni
Inductioni
By a mixture of inflammatory cytokines: TNF-alpha, IL-1 and interferon gamma.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000020407, Expressed in ectoplacental cone and 213 other tissues |
ExpressionAtlasi | P52624, baseline and differential |
Genevisiblei | P52624, MM |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
IntActi | P52624, 1 interactor |
MINTi | P52624 |
STRINGi | 10090.ENSMUSP00000020677 |
Chemistry databases
BindingDBi | P52624 |
Miscellaneous databases
RNActi | P52624, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 139 – 142 | Phosphate bindingBy similarity | 4 | |
Regioni | 143 – 144 | Uridine bindingBy similarity | 2 | |
Regioni | 218 – 220 | Uridine bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the PNP/UDP phosphorylase family.Curated
Phylogenomic databases
eggNOGi | KOG3728, Eukaryota |
GeneTreei | ENSGT00940000157781 |
HOGENOMi | CLU_054104_0_0_1 |
InParanoidi | P52624 |
OMAi | RMENFAY |
OrthoDBi | 1423938at2759 |
PhylomeDBi | P52624 |
TreeFami | TF314310 |
Family and domain databases
Gene3Di | 3.40.50.1580, 1 hit |
InterProi | View protein in InterPro IPR018016, Nucleoside_phosphorylase_CS IPR000845, Nucleoside_phosphorylase_d IPR035994, Nucleoside_phosphorylase_sf IPR010059, Uridine_phosphorylase_euk |
Pfami | View protein in Pfam PF01048, PNP_UDP_1, 1 hit |
SUPFAMi | SSF53167, SSF53167, 1 hit |
TIGRFAMsi | TIGR01719, euk_UDPppase, 1 hit |
PROSITEi | View protein in PROSITE PS01232, PNP_UDP_1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
P52624-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAATGTEAKD LENHHNDCFI QLSNPNIAAM KEDVLYHFNL STSTHDFPAM
60 70 80 90 100
FGDVKFVCVG GSSSRMNTFI KYVAAELGLD HPGKEYPNIC AGTDRYAMYK
110 120 130 140 150
AGPVLSVSHG MGIPSIGIML HELIKMLYHA RCSNITIIRI GTSGGIGLEP
160 170 180 190 200
GSVVITQQAV NECFKPEFEQ IVLGKRVIRN TNLDAQLVQE LVQCSSDLNE
210 220 230 240 250
FPMVVGNTMC TLDFYEGQGR LDGALCSYTE KDKQSYLRAA HAAGVRNIEM
260 270 280 290 300
ESSVFATMCS ACGLKAAVVC VTLLDRLQGD QINTPHDVLV EYQQRPQRLV
310
GHFIKKSLGR A
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q5K7 | E9Q5K7_MOUSE | Uridine phosphorylase 1 | Upp1 | 149 | Annotation score: | ||
F6YBT6 | F6YBT6_MOUSE | Uridine phosphorylase 1 | Upp1 | 143 | Annotation score: | ||
Q5SUC6 | Q5SUC6_MOUSE | Uridine phosphorylase 1 | Upp1 | 137 | Annotation score: | ||
E9Q391 | E9Q391_MOUSE | Uridine phosphorylase 1 | Upp1 | 114 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 260 | S → G in BAA07916 (PubMed:7744869).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D44464 mRNA Translation: BAA07916.1 BC036559 mRNA Translation: AAH36559.1 |
CCDSi | CCDS24432.1 |
PIRi | A57501 |
RefSeqi | NP_001152873.1, NM_001159401.1 NP_001152874.1, NM_001159402.1 NP_033503.2, NM_009477.3 XP_006514730.1, XM_006514667.2 |
Genome annotation databases
Ensembli | ENSMUST00000020677; ENSMUSP00000020677; ENSMUSG00000020407 ENSMUST00000101525; ENSMUSP00000099063; ENSMUSG00000020407 ENSMUST00000164791; ENSMUSP00000127473; ENSMUSG00000020407 |
GeneIDi | 22271 |
KEGGi | mmu:22271 |
UCSCi | uc007hzw.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D44464 mRNA Translation: BAA07916.1 BC036559 mRNA Translation: AAH36559.1 |
CCDSi | CCDS24432.1 |
PIRi | A57501 |
RefSeqi | NP_001152873.1, NM_001159401.1 NP_001152874.1, NM_001159402.1 NP_033503.2, NM_009477.3 XP_006514730.1, XM_006514667.2 |
3D structure databases
SMRi | P52624 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P52624, 1 interactor |
MINTi | P52624 |
STRINGi | 10090.ENSMUSP00000020677 |
Chemistry databases
BindingDBi | P52624 |
ChEMBLi | CHEMBL3718 |
PTM databases
PhosphoSitePlusi | P52624 |
SwissPalmi | P52624 |
Proteomic databases
jPOSTi | P52624 |
PaxDbi | P52624 |
PeptideAtlasi | P52624 |
PRIDEi | P52624 |
Protocols and materials databases
Antibodypediai | 27594, 164 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000020677; ENSMUSP00000020677; ENSMUSG00000020407 ENSMUST00000101525; ENSMUSP00000099063; ENSMUSG00000020407 ENSMUST00000164791; ENSMUSP00000127473; ENSMUSG00000020407 |
GeneIDi | 22271 |
KEGGi | mmu:22271 |
UCSCi | uc007hzw.2, mouse |
Organism-specific databases
CTDi | 7378 |
MGIi | MGI:1097668, Upp1 |
Phylogenomic databases
eggNOGi | KOG3728, Eukaryota |
GeneTreei | ENSGT00940000157781 |
HOGENOMi | CLU_054104_0_0_1 |
InParanoidi | P52624 |
OMAi | RMENFAY |
OrthoDBi | 1423938at2759 |
PhylomeDBi | P52624 |
TreeFami | TF314310 |
Enzyme and pathway databases
UniPathwayi | UPA00574;UER00633 |
Reactomei | R-MMU-73614, Pyrimidine salvage R-MMU-73621, Pyrimidine catabolism |
Miscellaneous databases
BioGRID-ORCSi | 22271, 2 hits in 18 CRISPR screens |
ChiTaRSi | Upp1, mouse |
PROi | PR:P52624 |
RNActi | P52624, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020407, Expressed in ectoplacental cone and 213 other tissues |
ExpressionAtlasi | P52624, baseline and differential |
Genevisiblei | P52624, MM |
Family and domain databases
Gene3Di | 3.40.50.1580, 1 hit |
InterProi | View protein in InterPro IPR018016, Nucleoside_phosphorylase_CS IPR000845, Nucleoside_phosphorylase_d IPR035994, Nucleoside_phosphorylase_sf IPR010059, Uridine_phosphorylase_euk |
Pfami | View protein in Pfam PF01048, PNP_UDP_1, 1 hit |
SUPFAMi | SSF53167, SSF53167, 1 hit |
TIGRFAMsi | TIGR01719, euk_UDPppase, 1 hit |
PROSITEi | View protein in PROSITE PS01232, PNP_UDP_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UPP1_MOUSE | |
Accessioni | P52624Primary (citable) accession number: P52624 Secondary accession number(s): Q8JZX0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | May 23, 2003 | |
Last modified: | December 2, 2020 | |
This is version 153 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families