UniProtKB - P52599 (EMRK_ECOLI)
Protein
Probable multidrug resistance protein EmrK
Gene
emrK
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the tripartite efflux system EmrYK-TolC, which confers resistance to various drugs.By similarity
GO - Molecular functioni
- xenobiotic transmembrane transporter activity Source: InterPro
GO - Biological processi
- bile acid and bile salt transport Source: EcoCyc
- cellular response to DNA damage stimulus Source: EcoCyc
- response to antibiotic Source: EcoCyc
- xenobiotic detoxification by transmembrane export across the plasma membrane Source: EcoCyc
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:G7233-MONOMER MetaCyc:G7233-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Probable multidrug resistance protein EmrK |
Gene namesi | Name:emrK Ordered Locus Names:b2368, JW2365 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane Curated; Single-pass membrane protein Curated; Periplasmic side Curated
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 16 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 17 – 37 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 38 – 387 | PeriplasmicSequence analysisAdd BLAST | 350 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutants are more sensitive to nalidixic acid, mitomycin C and other stresses such as hydrogen peroxide or UV irradiation.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201871 | 1 – 387 | Probable multidrug resistance protein EmrKAdd BLAST | 387 |
Proteomic databases
PaxDbi | P52599 |
PRIDEi | P52599 |
Expressioni
Inductioni
Growth phase-dependent transcription is induced by tetracycline. Expression may be controlled by both RpoS and the Mar system.1 Publication
Interactioni
Subunit structurei
Part of the tripartite efflux system EmrYK-TolC, which is composed of an inner membrane transporter, EmrY, a membrane fusion protein, EmrK, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 4260557, 148 interactors |
ComplexPortali | CPX-4273, EmrKY-TolC multidrug efflux transport system |
STRINGi | 511145.b2368 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1566, Bacteria |
HOGENOMi | CLU_018816_15_0_6 |
InParanoidi | P52599 |
PhylomeDBi | P52599 |
Family and domain databases
InterProi | View protein in InterPro IPR043602, CusB_dom_1 IPR005694, Efflux_pump_mem IPR032317, HlyD_D23 |
Pfami | View protein in Pfam PF00529, HlyD, 1 hit PF16576, HlyD_D23, 1 hit |
TIGRFAMsi | TIGR00998, 8a0101, 1 hit |
i Sequence
Sequence statusi: Complete.
P52599-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEQINSNKKH SNRRKYFSLL AVVLFIAFSG AYAYWSMELE DMISTDDAYV
60 70 80 90 100
TGNADPISAQ VSGSVTVVNH KDTNYVRQGD ILVSLDKTDA TIALNKAKNN
110 120 130 140 150
LANIVRQTNK LYLQDKQYSA EVASARIQYQ QSLEDYNRRV PLAKQGVISK
160 170 180 190 200
ETLEHTKDTL ISSKAALNAA IQAYKANKAL VMNTPLNRQP QVVEAADATK
210 220 230 240 250
EAWLALKRTD IKSPVTGYIA QRSVQVGETV SPGQSLMAVV PARQMWVNAN
260 270 280 290 300
FKETQLTDVR IGQSVNIISD LYGENVVFHG RVTGINMGTG NAFSLLPAQN
310 320 330 340 350
ATGNWIKIVQ RVPVEVSLDP KELMEHPLRI GLSMTATIDT KNEDIAEMPE
360 370 380
LASTVTSMPA YTSKALVIDT SPIEKEISNI ISHNGQL
Sequence cautioni
The sequence BAA11236 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D78168 Genomic DNA Translation: BAA11236.1 Different initiation. U00096 Genomic DNA Translation: AAC75427.1 AP009048 Genomic DNA Translation: BAA16239.2 |
PIRi | E65010 |
RefSeqi | NP_416869.1, NC_000913.3 WP_000435167.1, NZ_SSZK01000006.1 |
Genome annotation databases
EnsemblBacteriai | AAC75427; AAC75427; b2368 BAA16239; BAA16239; BAA16239 |
GeneIDi | 57728154 946840 |
KEGGi | ecj:JW2365 eco:b2368 |
PATRICi | fig|1411691.4.peg.4361 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D78168 Genomic DNA Translation: BAA11236.1 Different initiation. U00096 Genomic DNA Translation: AAC75427.1 AP009048 Genomic DNA Translation: BAA16239.2 |
PIRi | E65010 |
RefSeqi | NP_416869.1, NC_000913.3 WP_000435167.1, NZ_SSZK01000006.1 |
3D structure databases
SMRi | P52599 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260557, 148 interactors |
ComplexPortali | CPX-4273, EmrKY-TolC multidrug efflux transport system |
STRINGi | 511145.b2368 |
Proteomic databases
PaxDbi | P52599 |
PRIDEi | P52599 |
Genome annotation databases
EnsemblBacteriai | AAC75427; AAC75427; b2368 BAA16239; BAA16239; BAA16239 |
GeneIDi | 57728154 946840 |
KEGGi | ecj:JW2365 eco:b2368 |
PATRICi | fig|1411691.4.peg.4361 |
Organism-specific databases
EchoBASEi | EB3067 |
Phylogenomic databases
eggNOGi | COG1566, Bacteria |
HOGENOMi | CLU_018816_15_0_6 |
InParanoidi | P52599 |
PhylomeDBi | P52599 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7233-MONOMER MetaCyc:G7233-MONOMER |
Miscellaneous databases
PROi | PR:P52599 |
Family and domain databases
InterProi | View protein in InterPro IPR043602, CusB_dom_1 IPR005694, Efflux_pump_mem IPR032317, HlyD_D23 |
Pfami | View protein in Pfam PF00529, HlyD, 1 hit PF16576, HlyD_D23, 1 hit |
TIGRFAMsi | TIGR00998, 8a0101, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EMRK_ECOLI | |
Accessioni | P52599Primary (citable) accession number: P52599 Secondary accession number(s): P76517, P76944, P76945 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 139 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families