UniProtKB - P52487 (UBC84_DROME)
Protein
Ubiquitin-conjugating enzyme E2-18 kDa
Gene
Ubc84D
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Catalyzes the covalent attachment of ubiquitin to other proteins.PROSITE-ProRule annotation
Catalytic activityi
- S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation EC:2.3.2.23
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 86 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ubiquitin conjugating enzyme activity Source: GO_Central
- ubiquitin protein ligase binding Source: GO_Central
- ubiquitin-protein transferase activity Source: FlyBase
GO - Biological processi
- protein polyubiquitination Source: FlyBase
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DME-1169408, ISG15 antiviral mechanism R-DME-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | P52487 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-conjugating enzyme E2-18 kDa (EC:2.3.2.23)Alternative name(s): E2 ubiquitin-conjugating enzyme 84D Ubiquitin carrier protein Ubiquitin-protein ligase |
Gene namesi | Name:Ubc84D ORF Names:CG12799 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0017456, Ubc84D |
Subcellular locationi
Nucleus
- nucleus Source: GO_Central
Other locations
- ubiquitin ligase complex Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082524 | 1 – 153 | Ubiquitin-conjugating enzyme E2-18 kDaAdd BLAST | 153 |
Proteomic databases
PaxDbi | P52487 |
PRIDEi | P52487 |
Expressioni
Gene expression databases
Bgeei | FBgn0017456, Expressed in testis and 16 other tissues |
ExpressionAtlasi | P52487, baseline and differential |
Genevisiblei | P52487, DM |
Interactioni
GO - Molecular functioni
- ubiquitin protein ligase binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 66090, 9 interactors |
DIPi | DIP-17217N |
IntActi | P52487, 7 interactors |
STRINGi | 7227.FBpp0081076 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 149 | UBC corePROSITE-ProRule annotationAdd BLAST | 148 |
Sequence similaritiesi
Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0422, Eukaryota |
GeneTreei | ENSGT00940000165322 |
HOGENOMi | CLU_030988_13_3_1 |
InParanoidi | P52487 |
OMAi | IIHDPEP |
OrthoDBi | 1420213at2759 |
PhylomeDBi | P52487 |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P52487-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAATRRLTRE LSDLVEAKMS TLRNIESSDE SLLMWTGLLV PEKAPYNKGA
60 70 80 90 100
FRIEINFPPQ YPFMPPKILF KTKIYHPNVD EKGEVCLPII STDNWKPTTR
110 120 130 140 150
TEQVLQALVA IVHNPEPEHP LRSDLAEEFV REHKKFMKTA EEFTKKNAEK
RPE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U51051 Genomic DNA Translation: AAB01150.1 AE014297 Genomic DNA Translation: AAF54011.1 BT088773 mRNA Translation: ACR82498.1 |
RefSeqi | NP_524260.1, NM_079536.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0081552; FBpp0081076; FBgn0017456 |
GeneIDi | 40900 |
KEGGi | dme:Dmel_CG12799 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U51051 Genomic DNA Translation: AAB01150.1 AE014297 Genomic DNA Translation: AAF54011.1 BT088773 mRNA Translation: ACR82498.1 |
RefSeqi | NP_524260.1, NM_079536.3 |
3D structure databases
SMRi | P52487 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 66090, 9 interactors |
DIPi | DIP-17217N |
IntActi | P52487, 7 interactors |
STRINGi | 7227.FBpp0081076 |
Proteomic databases
PaxDbi | P52487 |
PRIDEi | P52487 |
Genome annotation databases
EnsemblMetazoai | FBtr0081552; FBpp0081076; FBgn0017456 |
GeneIDi | 40900 |
KEGGi | dme:Dmel_CG12799 |
Organism-specific databases
CTDi | 40900 |
FlyBasei | FBgn0017456, Ubc84D |
Phylogenomic databases
eggNOGi | KOG0422, Eukaryota |
GeneTreei | ENSGT00940000165322 |
HOGENOMi | CLU_030988_13_3_1 |
InParanoidi | P52487 |
OMAi | IIHDPEP |
OrthoDBi | 1420213at2759 |
PhylomeDBi | P52487 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-DME-1169408, ISG15 antiviral mechanism R-DME-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | P52487 |
Miscellaneous databases
BioGRID-ORCSi | 40900, 0 hits in 1 CRISPR screen |
GenomeRNAii | 40900 |
PROi | PR:P52487 |
Gene expression databases
Bgeei | FBgn0017456, Expressed in testis and 16 other tissues |
ExpressionAtlasi | P52487, baseline and differential |
Genevisiblei | P52487, DM |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBC84_DROME | |
Accessioni | P52487Primary (citable) accession number: P52487 Secondary accession number(s): C4XVG9, Q9VIB4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 18, 2001 | |
Last modified: | December 2, 2020 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families