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UniProtKB - P52485 (UBCD2_DROME)
Protein
Ubiquitin-conjugating enzyme E2-24 kDa
Gene
Ubc2
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Catalyzes the covalent attachment of ubiquitin to other proteins.
Catalytic activityi
- S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation EC:2.3.2.23
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 170 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ubiquitin conjugating enzyme activity Source: GO_Central
- ubiquitin-protein transferase activity Source: FlyBase
GO - Biological processi
- lipid storage Source: FlyBase
- protein polyubiquitination Source: FlyBase
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DME-141430, Inactivation of APC/C via direct inhibition of the APC/C complex R-DME-174048, APC/C:Cdc20 mediated degradation of Cyclin B R-DME-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-DME-174154, APC/C:Cdc20 mediated degradation of Securin R-DME-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-DME-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-DME-176407, Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase R-DME-176408, Regulation of APC/C activators between G1/S and early anaphase R-DME-176409, APC/C:Cdc20 mediated degradation of mitotic proteins R-DME-176412, Phosphorylation of the APC/C R-DME-179409, APC-Cdc20 mediated degradation of Nek2A R-DME-2467813, Separation of Sister Chromatids R-DME-2559582, Senescence-Associated Secretory Phenotype (SASP) R-DME-69017, CDK-mediated phosphorylation and removal of Cdc6 R-DME-8866652, Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-DME-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | P52485 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-conjugating enzyme E2-24 kDa (EC:2.3.2.23)Alternative name(s): E2 ubiquitin-conjugating enzyme 2 Ubiquitin carrier protein Ubiquitin-protein ligase |
Gene namesi | ORF Names:CG6720Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0015320, Ubc2 |
VEuPathDBi | VectorBase:FBgn0015320 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082520 | 1 – 232 | Ubiquitin-conjugating enzyme E2-24 kDaAdd BLAST | 232 |
Proteomic databases
PaxDbi | P52485 |
PRIDEi | P52485 |
PTM databases
SwissPalmi | P52485 |
Expressioni
Gene expression databases
Bgeei | FBgn0015320, Expressed in wing disc (Drosophila) and 48 other tissues |
ExpressionAtlasi | P52485, baseline and differential |
Genevisiblei | P52485, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 60561, 17 interactors |
DIPi | DIP-17329N |
IntActi | P52485, 15 interactors |
STRINGi | 7227.FBpp0305676 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 86 – 232 | UBC corePROSITE-ProRule annotationAdd BLAST | 147 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 87 | DisorderedSequence analysisAdd BLAST | 87 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 61 | Polar residuesSequence analysisAdd BLAST | 61 |
Sequence similaritiesi
Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0417, Eukaryota |
HOGENOMi | CLU_030988_14_0_1 |
InParanoidi | P52485 |
OMAi | GDRAKHD |
OrthoDBi | 1337945at2759 |
PhylomeDBi | P52485 |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P52485-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSTPAAGSA AEVATSSATS NAPSAPSTTA SNVSNTSQPT TAGTPQARGG
60 70 80 90 100
RGSNANGGAS GSNAGGGDEP RKEAKTTPRI SRALGTSAKR IQKELAEITL
110 120 130 140 150
DPPPNCSAGP KGDNLYEWVS TILGPPGSVY EGGVFFLDIH FSPEYPFKPP
160 170 180 190 200
KVTFRTRIYH CNINSQGVIC LDILKDNWSP ALTISKVLLS ICSLLTDCNP
210 220 230
ADPLVGSIAT QYLQNREEHD RIARLWTKRY AT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ541C3 | Q541C3_DROME | RE74673p | Ubc2 Dmel\CG6720, l(2)k13206, UBCD2, UbcD2, ubcD2 | 232 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X92663 mRNA Translation: CAA63351.1 AE014134 Genomic DNA Translation: AAF53008.1 |
RefSeqi | NP_001260362.1, NM_001273433.1 NP_001285814.1, NM_001298885.1 NP_477137.1, NM_057789.4 NP_723616.1, NM_164943.2 |
Genome annotation databases
EnsemblMetazoai | FBtr0080115; FBpp0079704; FBgn0015320 FBtr0080116; FBpp0079705; FBgn0015320 FBtr0333492; FBpp0305676; FBgn0015320 FBtr0345437; FBpp0311562; FBgn0015320 |
GeneIDi | 34487 |
KEGGi | dme:Dmel_CG6720 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X92663 mRNA Translation: CAA63351.1 AE014134 Genomic DNA Translation: AAF53008.1 |
RefSeqi | NP_001260362.1, NM_001273433.1 NP_001285814.1, NM_001298885.1 NP_477137.1, NM_057789.4 NP_723616.1, NM_164943.2 |
3D structure databases
AlphaFoldDBi | P52485 |
SMRi | P52485 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 60561, 17 interactors |
DIPi | DIP-17329N |
IntActi | P52485, 15 interactors |
STRINGi | 7227.FBpp0305676 |
PTM databases
SwissPalmi | P52485 |
Proteomic databases
PaxDbi | P52485 |
PRIDEi | P52485 |
Protocols and materials databases
DNASUi | 34487 |
Genome annotation databases
EnsemblMetazoai | FBtr0080115; FBpp0079704; FBgn0015320 FBtr0080116; FBpp0079705; FBgn0015320 FBtr0333492; FBpp0305676; FBgn0015320 FBtr0345437; FBpp0311562; FBgn0015320 |
GeneIDi | 34487 |
KEGGi | dme:Dmel_CG6720 |
Organism-specific databases
CTDi | 34487 |
FlyBasei | FBgn0015320, Ubc2 |
VEuPathDBi | VectorBase:FBgn0015320 |
Phylogenomic databases
eggNOGi | KOG0417, Eukaryota |
HOGENOMi | CLU_030988_14_0_1 |
InParanoidi | P52485 |
OMAi | GDRAKHD |
OrthoDBi | 1337945at2759 |
PhylomeDBi | P52485 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-DME-141430, Inactivation of APC/C via direct inhibition of the APC/C complex R-DME-174048, APC/C:Cdc20 mediated degradation of Cyclin B R-DME-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-DME-174154, APC/C:Cdc20 mediated degradation of Securin R-DME-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-DME-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-DME-176407, Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase R-DME-176408, Regulation of APC/C activators between G1/S and early anaphase R-DME-176409, APC/C:Cdc20 mediated degradation of mitotic proteins R-DME-176412, Phosphorylation of the APC/C R-DME-179409, APC-Cdc20 mediated degradation of Nek2A R-DME-2467813, Separation of Sister Chromatids R-DME-2559582, Senescence-Associated Secretory Phenotype (SASP) R-DME-69017, CDK-mediated phosphorylation and removal of Cdc6 R-DME-8866652, Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-DME-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | P52485 |
Miscellaneous databases
BioGRID-ORCSi | 34487, 1 hit in 3 CRISPR screens |
ChiTaRSi | Ubc2, fly |
GenomeRNAii | 34487 |
PROi | PR:P52485 |
Gene expression databases
Bgeei | FBgn0015320, Expressed in wing disc (Drosophila) and 48 other tissues |
ExpressionAtlasi | P52485, baseline and differential |
Genevisiblei | P52485, DM |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UBCD2_DROME | |
Accessioni | P52485Primary (citable) accession number: P52485 Secondary accession number(s): Q9VKQ4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families