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Protein

Ubiquitin-conjugating enzyme E2 E3

Gene

Ube2e3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination (By similarity). Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest.By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotationBy similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei145Glycyl thioester intermediate1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGrowth regulation, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 E3 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme E3
UbcM2
Ubiquitin carrier protein E3
Ubiquitin-conjugating enzyme E2-23 kDa
Ubiquitin-protein ligase E3
Gene namesi
Name:Ube2e3
Synonyms:Ubce4, Ubcm2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:107412 Ube2e3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi145C → S or A: Loss of enzymatic activity, interaction with IPO11, nuclear import, and effect on cell growth. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000824752 – 207Ubiquitin-conjugating enzyme E2 E3Add BLAST206

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei8PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP52483
PaxDbiP52483
PeptideAtlasiP52483
PRIDEiP52483

PTM databases

iPTMnetiP52483
PhosphoSitePlusiP52483
SwissPalmiP52483

Expressioni

Gene expression databases

BgeeiENSMUSG00000027011
ExpressionAtlasiP52483 baseline and differential
GenevisibleiP52483 MM

Interactioni

Subunit structurei

Interacts with NEDD4L. The ubiquitin-loaded form interacts specifically with importin-11 (IPO11), leading to its import into the nucleus.3 Publications

Protein-protein interaction databases

BioGridi204405, 9 interactors
IntActiP52483, 1 interactor
STRINGi10090.ENSMUSP00000028398

Structurei

3D structure databases

ProteinModelPortaliP52483
SMRiP52483
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00910000143990
HOGENOMiHOG000233455
HOVERGENiHBG063308
InParanoidiP52483
KOiK20217
OMAiGDRAKHD
OrthoDBiEOG091G0GF8
PhylomeDBiP52483
TreeFamiTF101117

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52483-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDRQRSDD ESPSTSSGSS DADQRDPAAP EPEEQEERKP SATQQKKNTK
60 70 80 90 100
LSSKTTAKLS TSAKRIQKEL AEITLDPPPN CSAGPKGDNI YEWRSTILGP
110 120 130 140 150
PGSVYEGGVF FLDITFSSDY PFKPPKVTFR TRIYHCNINS QGVICLDILK
160 170 180 190 200
DNWSPALTIS KVLLSICSLL TDCNPADPLV GSIATQYLTN RAEHDRIARQ

WTKRYAT
Length:207
Mass (Da):22,913
Last modified:May 24, 2004 - v2
Checksum:i821CB1382478DC9F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31E → K in CAA63352 (PubMed:8576256).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92664 mRNA Translation: CAA63352.1
AF003346 mRNA Translation: AAB60948.1
AK076011 mRNA Translation: BAC36118.1
AK168072 mRNA Translation: BAE40046.1
BC011477 mRNA Translation: AAH11477.1
CCDSiCCDS16167.1
RefSeqiNP_033480.1, NM_009454.2
XP_006499224.1, XM_006499161.3
XP_006499225.1, XM_006499162.3
XP_006499226.1, XM_006499163.3
UniGeneiMm.1485
Mm.393087

Genome annotation databases

EnsembliENSMUST00000028398; ENSMUSP00000028398; ENSMUSG00000027011
ENSMUST00000121433; ENSMUSP00000113463; ENSMUSG00000027011
GeneIDi22193
KEGGimmu:22193
UCSCiuc008kgm.1 mouse

Similar proteinsi

Entry informationi

Entry nameiUB2E3_MOUSE
AccessioniPrimary (citable) accession number: P52483
Secondary accession number(s): O09180, Q3TI00, Q91X63
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 24, 2004
Last modified: May 23, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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