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Protein

Ubiquitin-conjugating enzyme E2 E1

Gene

Ube2e1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination.By similarity

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotationBy similarity
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N6-monoubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei131Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.3.2.B8 3474
ReactomeiR-MMU-1169408 ISG15 antiviral mechanism
R-MMU-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-MMU-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-MMU-176408 Regulation of APC/C activators between G1/S and early anaphase
R-MMU-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-MMU-176412 Phosphorylation of the APC/C
R-MMU-179409 APC-Cdc20 mediated degradation of Nek2A
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-MMU-8866654 E3 ubiquitin ligases ubiquitinate target proteins
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 E1 (EC:2.3.2.23)
Alternative name(s):
(E3-independent) E2 ubiquitin-conjugating enzyme E1 (EC:2.3.2.24)
E2 ubiquitin-conjugating enzyme E1
UbcM3
Ubiquitin carrier protein E1
Ubiquitin-protein ligase E1
Gene namesi
Name:Ube2e1
Synonyms:Ubce5, Ubcm3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:107411 Ube2e1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000824712 – 193Ubiquitin-conjugating enzyme E2 E1Add BLAST192

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Cross-linki136Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)By similarity

Post-translational modificationi

ISGylation suppresses ubiquitin E2 enzyme activity.By similarity
Autoubiquitinated.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiP52482
PaxDbiP52482
PeptideAtlasiP52482
PRIDEiP52482

PTM databases

iPTMnetiP52482
PhosphoSitePlusiP52482
SwissPalmiP52482

Expressioni

Gene expression databases

BgeeiENSMUSG00000021774 Expressed in 196 organ(s), highest expression level in thymus
ExpressionAtlasiP52482 baseline and differential
GenevisibleiP52482 MM

Interactioni

Subunit structurei

Interacts with RNF14.

Protein-protein interaction databases

BioGridi204406, 3 interactors
IntActiP52482, 1 interactor
STRINGi10090.ENSMUSP00000022296

Structurei

3D structure databases

ProteinModelPortaliP52482
SMRiP52482
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi9 – 18Poly-Ser10

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00910000143990
HOGENOMiHOG000233455
HOVERGENiHBG063308
InParanoidiP52482
KOiK20217
OMAiIVFRTRI
OrthoDBiEOG091G0GF8
PhylomeDBiP52482
TreeFamiTF101117

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P52482-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDDDSRAST SSSSSSSSNQ QTEKEGSTPK KKESKVSMSK NSKLLSTSAK
60 70 80 90 100
RIQKELADIT LDPPPNCSAG PKGDNIYEWR STILGPPGSV YEGGVFFLDI
110 120 130 140 150
TFTPEYPFKP PKVTFRTRIY HCNINSQGVI CLDILKDNWS PALTISKVLL
160 170 180 190
SICSLLTDCN PADPLVGSIA TQYMTNRAEH DRMARQWTKR YAT
Length:193
Mass (Da):21,333
Last modified:October 1, 1996 - v1
Checksum:iB535B3095EF6C445
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A286YE31A0A286YE31_MOUSE
Ubiquitin-conjugating enzyme E2 E1
Ube2e1
148Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92665 mRNA Translation: CAA63353.1
BC003781 mRNA Translation: AAH03781.1
CCDSiCCDS26837.1
RefSeqiNP_033481.1, NM_009455.3
UniGeneiMm.4429

Genome annotation databases

EnsembliENSMUST00000022296; ENSMUSP00000022296; ENSMUSG00000021774
GeneIDi22194
KEGGimmu:22194
UCSCiuc007shu.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92665 mRNA Translation: CAA63353.1
BC003781 mRNA Translation: AAH03781.1
CCDSiCCDS26837.1
RefSeqiNP_033481.1, NM_009455.3
UniGeneiMm.4429

3D structure databases

ProteinModelPortaliP52482
SMRiP52482
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204406, 3 interactors
IntActiP52482, 1 interactor
STRINGi10090.ENSMUSP00000022296

PTM databases

iPTMnetiP52482
PhosphoSitePlusiP52482
SwissPalmiP52482

Proteomic databases

EPDiP52482
PaxDbiP52482
PeptideAtlasiP52482
PRIDEiP52482

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022296; ENSMUSP00000022296; ENSMUSG00000021774
GeneIDi22194
KEGGimmu:22194
UCSCiuc007shu.1 mouse

Organism-specific databases

CTDi7324
MGIiMGI:107411 Ube2e1

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00910000143990
HOGENOMiHOG000233455
HOVERGENiHBG063308
InParanoidiP52482
KOiK20217
OMAiIVFRTRI
OrthoDBiEOG091G0GF8
PhylomeDBiP52482
TreeFamiTF101117

Enzyme and pathway databases

UniPathwayi
UPA00143

BRENDAi2.3.2.B8 3474
ReactomeiR-MMU-1169408 ISG15 antiviral mechanism
R-MMU-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-MMU-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-MMU-176408 Regulation of APC/C activators between G1/S and early anaphase
R-MMU-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-MMU-176412 Phosphorylation of the APC/C
R-MMU-179409 APC-Cdc20 mediated degradation of Nek2A
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-MMU-8866654 E3 ubiquitin ligases ubiquitinate target proteins
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

PROiPR:P52482
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021774 Expressed in 196 organ(s), highest expression level in thymus
ExpressionAtlasiP52482 baseline and differential
GenevisibleiP52482 MM

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUB2E1_MOUSE
AccessioniPrimary (citable) accession number: P52482
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 12, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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