UniProtKB - P52435 (RPB11_HUMAN)
DNA-directed RNA polymerase II subunit RPB11-a
POLR2J
Functioni
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft (By similarity).
By similarity1 PublicationGO - Molecular functioni
- DNA binding Source: InterPro
- DNA-directed 5'-3' RNA polymerase activity Source: ProtInc
- LRR domain binding Source: UniProtKB
- protein dimerization activity Source: InterPro
- RNA polymerase II activity Source: InterPro
GO - Biological processi
- transcription by RNA polymerase II Source: UniProtKB
Keywordsi
Biological process | Transcription |
Enzyme and pathway databases
PathwayCommonsi | P52435 |
Reactomei | R-HSA-112382, Formation of RNA Pol II elongation complex R-HSA-113418, Formation of the Early Elongation Complex R-HSA-167152, Formation of HIV elongation complex in the absence of HIV Tat R-HSA-167158, Formation of the HIV-1 Early Elongation Complex R-HSA-167160, RNA Pol II CTD phosphorylation and interaction with CE during HIV infection R-HSA-167161, HIV Transcription Initiation R-HSA-167162, RNA Polymerase II HIV Promoter Escape R-HSA-167172, Transcription of the HIV genome R-HSA-167200, Formation of HIV-1 elongation complex containing HIV-1 Tat R-HSA-167238, Pausing and recovery of Tat-mediated HIV elongation R-HSA-167242, Abortive elongation of HIV-1 transcript in the absence of Tat R-HSA-167243, Tat-mediated HIV elongation arrest and recovery R-HSA-167246, Tat-mediated elongation of the HIV-1 transcript R-HSA-167287, HIV elongation arrest and recovery R-HSA-167290, Pausing and recovery of HIV elongation R-HSA-168325, Viral Messenger RNA Synthesis R-HSA-203927, MicroRNA (miRNA) biogenesis R-HSA-5578749, Transcriptional regulation by small RNAs R-HSA-5601884, PIWI-interacting RNA (piRNA) biogenesis R-HSA-5617472, Activation of anterior HOX genes in hindbrain development during early embryogenesis R-HSA-674695, RNA Polymerase II Pre-transcription Events R-HSA-6781823, Formation of TC-NER Pre-Incision Complex R-HSA-6781827, Transcription-Coupled Nucleotide Excision Repair (TC-NER) R-HSA-6782135, Dual incision in TC-NER R-HSA-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-HSA-6796648, TP53 Regulates Transcription of DNA Repair Genes R-HSA-6803529, FGFR2 alternative splicing R-HSA-6807505, RNA polymerase II transcribes snRNA genes R-HSA-72086, mRNA Capping R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-72165, mRNA Splicing - Minor Pathway R-HSA-72203, Processing of Capped Intron-Containing Pre-mRNA R-HSA-73776, RNA Polymerase II Promoter Escape R-HSA-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-HSA-75953, RNA Polymerase II Transcription Initiation R-HSA-75955, RNA Polymerase II Transcription Elongation R-HSA-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-HSA-77075, RNA Pol II CTD phosphorylation and interaction with CE R-HSA-8851708, Signaling by FGFR2 IIIa TM R-HSA-9018519, Estrogen-dependent gene expression R-HSA-9670095, Inhibition of DNA recombination at telomere |
SignaLinki | P52435 |
SIGNORi | P52435 |
Names & Taxonomyi
Protein namesi | Recommended name: DNA-directed RNA polymerase II subunit RPB11-aShort name: RNA polymerase II subunit B11-a Short name: RPB11a Alternative name(s): DNA-directed RNA polymerase II subunit J-1 RNA polymerase II 13.3 kDa subunit |
Gene namesi | Name:POLR2J Synonyms:POLR2J1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9197, POLR2J |
MIMi | 604150, gene |
neXtProti | NX_P52435 |
VEuPathDBi | HostDB:ENSG00000005075 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
- RNA polymerase II, core complex Source: UniProtKB
Keywords - Cellular componenti
DNA-directed RNA polymerase, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 5439 |
OpenTargetsi | ENSG00000005075 |
PharmGKBi | PA33517 |
Miscellaneous databases
Pharosi | P52435, Tbio |
Genetic variation databases
BioMutai | POLR2J |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000149309 | 1 – 117 | DNA-directed RNA polymerase II subunit RPB11-aAdd BLAST | 117 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionine1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | P52435 |
jPOSTi | P52435 |
MassIVEi | P52435 |
MaxQBi | P52435 |
PaxDbi | P52435 |
PeptideAtlasi | P52435 |
PRIDEi | P52435 |
ProteomicsDBi | 56486 |
PTM databases
iPTMneti | P52435 |
PhosphoSitePlusi | P52435 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000005075, Expressed in gastrocnemius and 126 other tissues |
ExpressionAtlasi | P52435, baseline and differential |
Genevisiblei | P52435, HS |
Organism-specific databases
HPAi | ENSG00000005075, Low tissue specificity |
Interactioni
Subunit structurei
Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.
Interacts with AATF.
2 PublicationsBinary interactionsi
P52435
GO - Molecular functioni
- LRR domain binding Source: UniProtKB
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 111435, 132 interactors |
CORUMi | P52435 |
DIPi | DIP-32913N |
IntActi | P52435, 49 interactors |
MINTi | P52435 |
STRINGi | 9606.ENSP00000292614 |
Miscellaneous databases
RNActi | P52435, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4392, Eukaryota |
GeneTreei | ENSGT00940000164393 |
HOGENOMi | CLU_090381_2_2_1 |
InParanoidi | P52435 |
OMAi | CTKLIVQ |
OrthoDBi | 1398114at2759 |
PhylomeDBi | P52435 |
TreeFami | TF103044 |
Family and domain databases
CDDi | cd06926, RNAP_II_RPB11, 1 hit |
Gene3Di | 3.30.1360.10, 1 hit |
HAMAPi | MF_00261, RNApol_arch_Rpo11, 1 hit |
InterProi | View protein in InterPro IPR037685, RBP11 IPR036603, RBP11-like IPR009025, RBP11-like_dimer IPR008193, RNA_pol_Rpb11_13-16kDa_CS |
Pfami | View protein in Pfam PF13656, RNA_pol_L_2, 1 hit |
SUPFAMi | SSF55257, SSF55257, 1 hit |
PROSITEi | View protein in PROSITE PS01154, RNA_POL_L_13KD, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MNAPPAFESF LLFEGEKKIT INKDTKVPNA CLFTINKEDH TLGNIIKSQL
60 70 80 90 100
LKDPQVLFAG YKVPHPLEHK IIIRVQTTPD YSPQEAFTNA ITDLISELSL
110
LEERFRVAIK DKQEGIE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF6S314 | F6S314_HUMAN | DNA-directed RNA polymerase II subu... | POLR2J | 158 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X82385 mRNA Translation: CAA57785.1 L37127 mRNA Translation: AAD05361.1 X98433 mRNA Translation: CAA67075.1 AK290515 mRNA Translation: BAF83204.1 AC093668 Genomic DNA No translation available. BC024165 mRNA Translation: AAH24165.1 BC065711 mRNA Translation: AAH65711.1 BC139902 mRNA Translation: AAI39903.1 BC141830 mRNA Translation: AAI41831.1 |
CCDSi | CCDS5724.1 |
PIRi | S71325 |
RefSeqi | NP_006225.1, NM_006234.4 |
Genome annotation databases
Ensembli | ENST00000292614; ENSP00000292614; ENSG00000005075 |
GeneIDi | 5439 |
KEGGi | hsa:5439 |
MANE-Selecti | ENST00000292614.10; ENSP00000292614.5; NM_006234.6; NP_006225.1 |
UCSCi | uc003uzp.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X82385 mRNA Translation: CAA57785.1 L37127 mRNA Translation: AAD05361.1 X98433 mRNA Translation: CAA67075.1 AK290515 mRNA Translation: BAF83204.1 AC093668 Genomic DNA No translation available. BC024165 mRNA Translation: AAH24165.1 BC065711 mRNA Translation: AAH65711.1 BC139902 mRNA Translation: AAI39903.1 BC141830 mRNA Translation: AAI41831.1 |
CCDSi | CCDS5724.1 |
PIRi | S71325 |
RefSeqi | NP_006225.1, NM_006234.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5IY6 | electron microscopy | 7.20 | K | 1-117 | [»] | |
5IY7 | electron microscopy | 8.60 | K | 1-117 | [»] | |
5IY8 | electron microscopy | 7.90 | K | 1-117 | [»] | |
5IY9 | electron microscopy | 6.30 | K | 1-117 | [»] | |
5IYA | electron microscopy | 5.40 | K | 1-117 | [»] | |
5IYB | electron microscopy | 3.90 | K | 1-117 | [»] | |
5IYC | electron microscopy | 3.90 | K | 1-117 | [»] | |
5IYD | electron microscopy | 3.90 | K | 1-117 | [»] | |
6DRD | electron microscopy | 3.90 | K | 1-117 | [»] | |
6O9L | electron microscopy | 7.20 | K | 1-117 | [»] | |
6XRE | electron microscopy | 4.60 | K | 1-117 | [»] | |
7LBM | electron microscopy | 4.80 | K | 1-117 | [»] | |
SMRi | P52435 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111435, 132 interactors |
CORUMi | P52435 |
DIPi | DIP-32913N |
IntActi | P52435, 49 interactors |
MINTi | P52435 |
STRINGi | 9606.ENSP00000292614 |
PTM databases
iPTMneti | P52435 |
PhosphoSitePlusi | P52435 |
Genetic variation databases
BioMutai | POLR2J |
Proteomic databases
EPDi | P52435 |
jPOSTi | P52435 |
MassIVEi | P52435 |
MaxQBi | P52435 |
PaxDbi | P52435 |
PeptideAtlasi | P52435 |
PRIDEi | P52435 |
ProteomicsDBi | 56486 |
Protocols and materials databases
Antibodypediai | 31080, 295 antibodies from 27 providers |
DNASUi | 5439 |
Genome annotation databases
Ensembli | ENST00000292614; ENSP00000292614; ENSG00000005075 |
GeneIDi | 5439 |
KEGGi | hsa:5439 |
MANE-Selecti | ENST00000292614.10; ENSP00000292614.5; NM_006234.6; NP_006225.1 |
UCSCi | uc003uzp.2, human |
Organism-specific databases
CTDi | 5439 |
DisGeNETi | 5439 |
GeneCardsi | POLR2J |
HGNCi | HGNC:9197, POLR2J |
HPAi | ENSG00000005075, Low tissue specificity |
MIMi | 604150, gene |
neXtProti | NX_P52435 |
OpenTargetsi | ENSG00000005075 |
PharmGKBi | PA33517 |
VEuPathDBi | HostDB:ENSG00000005075 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4392, Eukaryota |
GeneTreei | ENSGT00940000164393 |
HOGENOMi | CLU_090381_2_2_1 |
InParanoidi | P52435 |
OMAi | CTKLIVQ |
OrthoDBi | 1398114at2759 |
PhylomeDBi | P52435 |
TreeFami | TF103044 |
Enzyme and pathway databases
PathwayCommonsi | P52435 |
Reactomei | R-HSA-112382, Formation of RNA Pol II elongation complex R-HSA-113418, Formation of the Early Elongation Complex R-HSA-167152, Formation of HIV elongation complex in the absence of HIV Tat R-HSA-167158, Formation of the HIV-1 Early Elongation Complex R-HSA-167160, RNA Pol II CTD phosphorylation and interaction with CE during HIV infection R-HSA-167161, HIV Transcription Initiation R-HSA-167162, RNA Polymerase II HIV Promoter Escape R-HSA-167172, Transcription of the HIV genome R-HSA-167200, Formation of HIV-1 elongation complex containing HIV-1 Tat R-HSA-167238, Pausing and recovery of Tat-mediated HIV elongation R-HSA-167242, Abortive elongation of HIV-1 transcript in the absence of Tat R-HSA-167243, Tat-mediated HIV elongation arrest and recovery R-HSA-167246, Tat-mediated elongation of the HIV-1 transcript R-HSA-167287, HIV elongation arrest and recovery R-HSA-167290, Pausing and recovery of HIV elongation R-HSA-168325, Viral Messenger RNA Synthesis R-HSA-203927, MicroRNA (miRNA) biogenesis R-HSA-5578749, Transcriptional regulation by small RNAs R-HSA-5601884, PIWI-interacting RNA (piRNA) biogenesis R-HSA-5617472, Activation of anterior HOX genes in hindbrain development during early embryogenesis R-HSA-674695, RNA Polymerase II Pre-transcription Events R-HSA-6781823, Formation of TC-NER Pre-Incision Complex R-HSA-6781827, Transcription-Coupled Nucleotide Excision Repair (TC-NER) R-HSA-6782135, Dual incision in TC-NER R-HSA-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-HSA-6796648, TP53 Regulates Transcription of DNA Repair Genes R-HSA-6803529, FGFR2 alternative splicing R-HSA-6807505, RNA polymerase II transcribes snRNA genes R-HSA-72086, mRNA Capping R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-72165, mRNA Splicing - Minor Pathway R-HSA-72203, Processing of Capped Intron-Containing Pre-mRNA R-HSA-73776, RNA Polymerase II Promoter Escape R-HSA-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-HSA-75953, RNA Polymerase II Transcription Initiation R-HSA-75955, RNA Polymerase II Transcription Elongation R-HSA-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-HSA-77075, RNA Pol II CTD phosphorylation and interaction with CE R-HSA-8851708, Signaling by FGFR2 IIIa TM R-HSA-9018519, Estrogen-dependent gene expression R-HSA-9670095, Inhibition of DNA recombination at telomere |
SignaLinki | P52435 |
SIGNORi | P52435 |
Miscellaneous databases
BioGRID-ORCSi | 5439, 416 hits in 628 CRISPR screens |
ChiTaRSi | POLR2J, human |
GeneWikii | POLR2J |
GenomeRNAii | 5439 |
Pharosi | P52435, Tbio |
PROi | PR:P52435 |
RNActi | P52435, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000005075, Expressed in gastrocnemius and 126 other tissues |
ExpressionAtlasi | P52435, baseline and differential |
Genevisiblei | P52435, HS |
Family and domain databases
CDDi | cd06926, RNAP_II_RPB11, 1 hit |
Gene3Di | 3.30.1360.10, 1 hit |
HAMAPi | MF_00261, RNApol_arch_Rpo11, 1 hit |
InterProi | View protein in InterPro IPR037685, RBP11 IPR036603, RBP11-like IPR009025, RBP11-like_dimer IPR008193, RNA_pol_Rpb11_13-16kDa_CS |
Pfami | View protein in Pfam PF13656, RNA_pol_L_2, 1 hit |
SUPFAMi | SSF55257, SSF55257, 1 hit |
PROSITEi | View protein in PROSITE PS01154, RNA_POL_L_13KD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RPB11_HUMAN | |
Accessioni | P52435Primary (citable) accession number: P52435 Secondary accession number(s): A5D6V8, O43375 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 196 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families