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Protein

DNA-directed RNA polymerase II subunit RPB11-a

Gene

POLR2J

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft (By similarity).By similarity1 Publication

GO - Molecular functioni

  • DNA binding Source: InterPro
  • DNA-directed 5'-3' RNA polymerase activity Source: ProtInc
  • LRR domain binding Source: UniProtKB
  • protein dimerization activity Source: InterPro
  • RNA polymerase II activity Source: InterPro

GO - Biological processi

Keywordsi

Biological processTranscription

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-203927 MicroRNA (miRNA) biogenesis
R-HSA-452723 Transcriptional regulation of pluripotent stem cells
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-5601884 PIWI-interacting RNA (piRNA) biogenesis
R-HSA-5617472 Activation of anterior HOX genes in hindbrain development during early embryogenesis
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6781823 Formation of TC-NER Pre-Incision Complex
R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6803529 FGFR2 alternative splicing
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-72086 mRNA Capping
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8851708 Signaling by FGFR2 IIIa TM
R-HSA-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit RPB11-a
Short name:
RNA polymerase II subunit B11-a
Short name:
RPB11a
Alternative name(s):
DNA-directed RNA polymerase II subunit J-1
RNA polymerase II 13.3 kDa subunit
Gene namesi
Name:POLR2J
Synonyms:POLR2J1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000005075.15
HGNCiHGNC:9197 POLR2J
MIMi604150 gene
neXtProtiNX_P52435

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5439
OpenTargetsiENSG00000005075
PharmGKBiPA33517

Polymorphism and mutation databases

BioMutaiPOLR2J

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001493091 – 117DNA-directed RNA polymerase II subunit RPB11-aAdd BLAST117

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP52435
MaxQBiP52435
PaxDbiP52435
PeptideAtlasiP52435
PRIDEiP52435
ProteomicsDBi56486

PTM databases

iPTMnetiP52435
PhosphoSitePlusiP52435

Expressioni

Tissue specificityi

Ubiquitously expressed. High expression was found in heart and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000005075 Expressed in 102 organ(s), highest expression level in gastrocnemius
CleanExiHS_POLR2J
ExpressionAtlasiP52435 baseline and differential
GenevisibleiP52435 HS

Organism-specific databases

HPAiHPA044010
HPA046970

Interactioni

Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. Interacts with AATF.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
POLR1CO151608EBI-394753,EBI-1055079

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111435, 84 interactors
CORUMiP52435
DIPiDIP-32913N
IntActiP52435, 32 interactors
MINTiP52435
STRINGi9606.ENSP00000292614

Structurei

3D structure databases

ProteinModelPortaliP52435
SMRiP52435
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4392 Eukaryota
COG1761 LUCA
GeneTreeiENSGT00550000074975
HOGENOMiHOG000179862
HOVERGENiHBG073419
InParanoidiP52435
KOiK03008
OMAiEDTKIPN
PhylomeDBiP52435
TreeFamiTF103044

Family and domain databases

CDDicd06926 RNAP_II_RPB11, 1 hit
Gene3Di3.30.1360.10, 1 hit
InterProiView protein in InterPro
IPR037685 RBP11
IPR036603 RBP11-like
IPR009025 RBP11-like_dimer
IPR008193 RNA_pol_Rpb11_13-16kDa_CS
PfamiView protein in Pfam
PF13656 RNA_pol_L_2, 1 hit
SUPFAMiSSF55257 SSF55257, 1 hit
PROSITEiView protein in PROSITE
PS01154 RNA_POL_L_13KD, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P52435-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNAPPAFESF LLFEGEKKIT INKDTKVPNA CLFTINKEDH TLGNIIKSQL
60 70 80 90 100
LKDPQVLFAG YKVPHPLEHK IIIRVQTTPD YSPQEAFTNA ITDLISELSL
110
LEERFRVAIK DKQEGIE
Length:117
Mass (Da):13,293
Last modified:October 1, 1996 - v1
Checksum:i6F78AB256EF8F647
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6S314F6S314_HUMAN
DNA-directed RNA polymerase II subu...
POLR2J
158Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82385 mRNA Translation: CAA57785.1
L37127 mRNA Translation: AAD05361.1
X98433 mRNA Translation: CAA67075.1
AK290515 mRNA Translation: BAF83204.1
AC093668 Genomic DNA No translation available.
BC024165 mRNA Translation: AAH24165.1
BC065711 mRNA Translation: AAH65711.1
BC139902 mRNA Translation: AAI39903.1
BC141830 mRNA Translation: AAI41831.1
CCDSiCCDS5724.1
PIRiS71325
RefSeqiNP_006225.1, NM_006234.4
UniGeneiHs.654952

Genome annotation databases

EnsembliENST00000292614; ENSP00000292614; ENSG00000005075
GeneIDi5439
KEGGihsa:5439
UCSCiuc003uzp.2 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82385 mRNA Translation: CAA57785.1
L37127 mRNA Translation: AAD05361.1
X98433 mRNA Translation: CAA67075.1
AK290515 mRNA Translation: BAF83204.1
AC093668 Genomic DNA No translation available.
BC024165 mRNA Translation: AAH24165.1
BC065711 mRNA Translation: AAH65711.1
BC139902 mRNA Translation: AAI39903.1
BC141830 mRNA Translation: AAI41831.1
CCDSiCCDS5724.1
PIRiS71325
RefSeqiNP_006225.1, NM_006234.4
UniGeneiHs.654952

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IY6electron microscopy7.20K1-117[»]
5IY7electron microscopy8.60K1-117[»]
5IY8electron microscopy7.90K1-117[»]
5IY9electron microscopy6.30K1-117[»]
5IYAelectron microscopy5.40K1-117[»]
5IYBelectron microscopy3.90K1-117[»]
5IYCelectron microscopy3.90K1-117[»]
5IYDelectron microscopy3.90K1-117[»]
ProteinModelPortaliP52435
SMRiP52435
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111435, 84 interactors
CORUMiP52435
DIPiDIP-32913N
IntActiP52435, 32 interactors
MINTiP52435
STRINGi9606.ENSP00000292614

PTM databases

iPTMnetiP52435
PhosphoSitePlusiP52435

Polymorphism and mutation databases

BioMutaiPOLR2J

Proteomic databases

EPDiP52435
MaxQBiP52435
PaxDbiP52435
PeptideAtlasiP52435
PRIDEiP52435
ProteomicsDBi56486

Protocols and materials databases

DNASUi5439
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292614; ENSP00000292614; ENSG00000005075
GeneIDi5439
KEGGihsa:5439
UCSCiuc003uzp.2 human

Organism-specific databases

CTDi5439
DisGeNETi5439
EuPathDBiHostDB:ENSG00000005075.15
GeneCardsiPOLR2J
H-InvDBiHIX0025314
HGNCiHGNC:9197 POLR2J
HPAiHPA044010
HPA046970
MIMi604150 gene
neXtProtiNX_P52435
OpenTargetsiENSG00000005075
PharmGKBiPA33517
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4392 Eukaryota
COG1761 LUCA
GeneTreeiENSGT00550000074975
HOGENOMiHOG000179862
HOVERGENiHBG073419
InParanoidiP52435
KOiK03008
OMAiEDTKIPN
PhylomeDBiP52435
TreeFamiTF103044

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-203927 MicroRNA (miRNA) biogenesis
R-HSA-452723 Transcriptional regulation of pluripotent stem cells
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-5601884 PIWI-interacting RNA (piRNA) biogenesis
R-HSA-5617472 Activation of anterior HOX genes in hindbrain development during early embryogenesis
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6781823 Formation of TC-NER Pre-Incision Complex
R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6803529 FGFR2 alternative splicing
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-72086 mRNA Capping
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8851708 Signaling by FGFR2 IIIa TM
R-HSA-9018519 Estrogen-dependent gene expression

Miscellaneous databases

ChiTaRSiPOLR2J human
GeneWikiiPOLR2J
GenomeRNAii5439
PROiPR:P52435
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000005075 Expressed in 102 organ(s), highest expression level in gastrocnemius
CleanExiHS_POLR2J
ExpressionAtlasiP52435 baseline and differential
GenevisibleiP52435 HS

Family and domain databases

CDDicd06926 RNAP_II_RPB11, 1 hit
Gene3Di3.30.1360.10, 1 hit
InterProiView protein in InterPro
IPR037685 RBP11
IPR036603 RBP11-like
IPR009025 RBP11-like_dimer
IPR008193 RNA_pol_Rpb11_13-16kDa_CS
PfamiView protein in Pfam
PF13656 RNA_pol_L_2, 1 hit
SUPFAMiSSF55257 SSF55257, 1 hit
PROSITEiView protein in PROSITE
PS01154 RNA_POL_L_13KD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPB11_HUMAN
AccessioniPrimary (citable) accession number: P52435
Secondary accession number(s): A5D6V8, O43375
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 179 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
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