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UniProtKB - P52429 (DGKE_HUMAN)

Entry version 183 (10 Feb 2021)
Sequence version 1 (01 Oct 1996)
Previous versions | rss
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Protein

Diacylglycerol kinase epsilon

Gene

DGKE

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:15544348, PubMed:19744926, PubMed:22108654, PubMed:21477596, PubMed:23949095). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:8626589, PubMed:15544348). Also plays an important role in the biosynthesis of complex lipids (PubMed:8626589). Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle (PubMed:19744926, PubMed:22108654, PubMed:23274426). Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently (PubMed:22108654).2 Publications6 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Undergoes competitive inhibition by its own product 1,2-diacyl-sn-glycero-3-phosphate/phosphatidic acid. The strongest inhibition being observed in vitro with 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate, a major intermediate in the phosphatidylinositol turnover cycle and more generally by diacylglycerols with an arachidonoyl acyl chain at the sn-2 position.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glycerolipid metabolism

This protein is involved in the pathway glycerolipid metabolism, which is part of Lipid metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway glycerolipid metabolism and in Lipid metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri59 – 108Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST50
Zinc fingeri124 – 177Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST54

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.7.1.107, 2681

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		P52429

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-114508, Effects of PIP2 hydrolysis

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00230

Chemistry databases

SwissLipids knowledge resource for lipid biology

More...SwissLipidsi		SLP:000000546

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Diacylglycerol kinase epsilon (EC:2.7.1.1076 Publications)
Short name:
DAG kinase epsilon
Alternative name(s):
Diglyceride kinase epsilon
Short name:
DGK-epsilon
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:DGKE
Synonyms:DAGK5
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 17

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:2852, DGKE

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		601440, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_P52429

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000153933.9

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei22 – 42HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cytoplasm, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section provides information on the disease(s) associated with genetic variations in a given protein. The information is extracted from the scientific literature and diseases that are also described in the <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim">OMIM</a> database are represented with a <a href="http://www.uniprot.org/diseases">controlled vocabulary</a> in the following way:<p><a href='/help/involvement_in_disease' target='_top'>More...</a></p>Involvement in diseasei

Nephrotic syndrome 7 (NPHS7)1 Publication
The disease is caused by variants affecting the gene represented in this entry.
Disease descriptionA form of nephrotic syndrome, a renal disease clinically characterized by severe proteinuria, resulting in complications such as hypoalbuminemia, hyperlipidemia and edema. NPHS7 is an autosomal recessive form characterized by onset of proteinuria usually in the first decade of life. The disorder is progressive, and some patients develop end-stage renal disease within several years. Renal biopsy typically shows membranoproliferative glomerulonephritis.
Related information in OMIM
Hemolytic uremic syndrome atypical 7 (AHUS7)1 Publication
Disease susceptibility is associated with variants affecting the gene represented in this entry.
Disease descriptionAn atypical form of hemolytic uremic syndrome characterized by acute onset in the first year of life of microangiopathic hemolytic anemia, thrombocytopenia, and renal failure. After the acute episode, most patients develop chronic renal insufficiency. Unlike other genetic forms of aHUS, AHUS7 is not related to abnormal activation of the complement system.
Related information in OMIM
Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_06980463R → P in AHUS7. 1 PublicationCorresponds to variant dbSNP:rs312262694EnsemblClinVar.1
Natural variantiVAR_069805273R → P in AHUS7. 1 PublicationCorresponds to variant dbSNP:rs312262695EnsemblClinVar.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi431L → I: Decreased diacylglycerol kinase activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol. 1 Publication1
Mutagenesisi431L → S: Loss of diacylglycerol kinase activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol. 1 Publication1
Mutagenesisi438L → I: Decreased diacylglycerol kinase activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol. 1 Publication1
Mutagenesisi438L → M: Decreased protein abundance and diacylglycerol kinase activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol. 1 Publication1
Mutagenesisi438L → S, A or G: Loss of diacylglycerol kinase activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol. 1 Publication1
Mutagenesisi439P → G: Decreased diacylglycerol kinase activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol. 1 Publication1

Keywords - Diseasei

Disease variant, Hemolytic uremic syndrome

Organism-specific databases

DisGeNET

More...DisGeNETi		8526

GeneReviews a resource of expert-authored, peer-reviewed disease descriptions.

More...GeneReviewsi		DGKE

MalaCards human disease database

More...MalaCardsi		DGKE
MIMi615008, phenotype

Open Targets

More...OpenTargetsi		ENSG00000153933

Orphanet; a database dedicated to information on rare diseases and orphan drugs

More...Orphaneti		357008, Hemolytic uremic syndrome with DGKE deficiency
329903, Immunoglobulin-mediated membranoproliferative glomerulonephritis

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA27313

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		P52429, Tbio

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL1075187

Drug and drug target database

More...DrugBanki		DB14001, alpha-Tocopherol succinate

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		DGKE

Domain mapping of disease mutations (DMDM)

More...DMDMi		1708625

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002184641 – 567Diacylglycerol kinase epsilonAdd BLAST567

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		P52429

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P52429

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		P52429

MaxQB - The MaxQuant DataBase

More...MaxQBi		P52429

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P52429

PeptideAtlas

More...PeptideAtlasi		P52429

PRoteomics IDEntifications database

More...PRIDEi		P52429

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		56484 [P52429-1]
74029

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P52429

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		P52429

SwissPalm database of S-palmitoylation events

More...SwissPalmi		P52429

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level).1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000153933, Expressed in middle temporal gyrus and 207 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		P52429, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		P52429, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000153933, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		114096, 34 interactors

Protein interaction database and analysis system

More...IntActi		P52429, 11 interactors

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000284061

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P52429, protein

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini215 – 356DAGKcPROSITE-ProRule annotationAdd BLAST142

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the eukaryotic diacylglycerol kinase family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri59 – 108Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST50
Zinc fingeri124 – 177Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST54

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG1169, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000158604

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_1517427_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P52429

Identification of Orthologs from Complete Genome Data

More...OMAi		WVLNTIY

Database of Orthologous Groups

More...OrthoDBi		1275907at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		P52429

TreeFam database of animal gene trees

More...TreeFami		TF313104

Family and domain databases

Conserved Domains Database

More...CDDi		cd00029, C1, 2 hits

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.40.50.10330, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR017438, ATP-NAD_kinase_N
IPR037607, DGK
IPR000756, Diacylglycerol_kin_accessory
IPR001206, Diacylglycerol_kinase_cat_dom
IPR016064, NAD/diacylglycerol_kinase_sf
IPR002219, PE/DAG-bd

The PANTHER Classification System

More...PANTHERi		PTHR11255, PTHR11255, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00130, C1_1, 1 hit
PF00609, DAGK_acc, 1 hit
PF00781, DAGK_cat, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00109, C1, 2 hits
SM00045, DAGKa, 1 hit
SM00046, DAGKc, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF111331, SSF111331, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50146, DAGK, 1 hit
PS00479, ZF_DAG_PE_1, 2 hits
PS50081, ZF_DAG_PE_2, 2 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P52429-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEAERRPAPG SPSEGLFADG HLILWTLCSV LLPVFITFWC SLQRSRRQLH 
        60         70         80         90        100
RRDIFRKSKH GWRDTDLFSQ PTYCCVCAQH ILQGAFCDCC GLRVDEGCLR 
       110        120        130        140        150
KADKRFQCKE IMLKNDTKVL DAMPHHWIRG NVPLCSYCMV CKQQCGCQPK 
       160        170        180        190        200
LCDYRCIWCQ KTVHDECMKN SLKNEKCDFG EFKNLIIPPS YLTSINQMRK 
       210        220        230        240        250
DKKTDYEVLA SKLGKQWTPL IILANSRSGT NMGEGLLGEF RILLNPVQVF 
       260        270        280        290        300
DVTKTPPIKA LQLCTLLPYY SARVLVCGGD GTVGWVLDAV DDMKIKGQEK 
       310        320        330        340        350
YIPQVAVLPL GTGNDLSNTL GWGTGYAGEI PVAQVLRNVM EADGIKLDRW 
       360        370        380        390        400
KVQVTNKGYY NLRKPKEFTM NNYFSVGPDA LMALNFHAHR EKAPSLFSSR 
       410        420        430        440        450
ILNKAVYLFY GTKDCLVQEC KDLNKKVELE LDGERVALPS LEGIIVLNIG 
       460        470        480        490        500
YWGGGCRLWE GMGDETYPLA RHDDGLLEVV GVYGSFHCAQ IQVKLANPFR 
       510        520        530        540        550
IGQAHTVRLI LKCSMMPMQV DGEPWAQGPC TVTITHKTHA MMLYFSGEQT 
       560 
DDDISSTSDQ EDIKATE
Length:567
Mass (Da):63,927
Last modified:October 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iBC334AD15FB4D0B4
GO
Isoform 2 (identifier: P52429-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     156-177: CIWCQKTVHDECMKNSLKNEKC → YGLRGHSLSQNAPWESGFHRVV
     178-567: Missing.

Show »
Length:177
Mass (Da):20,452
Checksum:i185B610815D0859A
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
I3L112I3L112_HUMAN
Diacylglycerol kinase
DGKE
456Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
I3L2V2I3L2V2_HUMAN
Diacylglycerol kinase epsilon
DGKE
70Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06980463R → P in AHUS7. 1 PublicationCorresponds to variant dbSNP:rs312262694EnsemblClinVar.1
Natural variantiVAR_03612099L → R in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_069805273R → P in AHUS7. 1 PublicationCorresponds to variant dbSNP:rs312262695EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_056957156 – 177CIWCQ…KNEKC → YGLRGHSLSQNAPWESGFHR VV in isoform 2. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_056958178 – 567Missing in isoform 2. 1 PublicationAdd BLAST390

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U49379 mRNA Translation: AAC50497.1
AC015912 Genomic DNA No translation available.
AC106858 Genomic DNA No translation available.
BC022297 mRNA Translation: AAH22297.1
AF136745 Genomic DNA Translation: AAD45666.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS11590.1 [P52429-1]

NCBI Reference Sequences

More...RefSeqi		NP_003638.1, NM_003647.2 [P52429-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000284061; ENSP00000284061; ENSG00000153933 [P52429-1]
ENST00000572810; ENSP00000459295; ENSG00000153933 [P52429-2]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		8526

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:8526

UCSC genome browser

More...UCSCi		uc002iur.4, human [P52429-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49379 mRNA Translation: AAC50497.1
AC015912 Genomic DNA No translation available.
AC106858 Genomic DNA No translation available.
BC022297 mRNA Translation: AAH22297.1
AF136745 Genomic DNA Translation: AAD45666.1
CCDSiCCDS11590.1 [P52429-1]
RefSeqiNP_003638.1, NM_003647.2 [P52429-1]

3D structure databases

Database of comparative protein structure models

More...ModBasei		Search...

SWISS-MODEL Interactive Workspace

More...SWISS-MODEL-Workspacei		Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi114096, 34 interactors
IntActiP52429, 11 interactors
STRINGi9606.ENSP00000284061

Chemistry databases

ChEMBLiCHEMBL1075187
DrugBankiDB14001, alpha-Tocopherol succinate
SwissLipidsiSLP:000000546

PTM databases

iPTMnetiP52429
PhosphoSitePlusiP52429
SwissPalmiP52429

Genetic variation databases

BioMutaiDGKE
DMDMi1708625

Proteomic databases

EPDiP52429
jPOSTiP52429
MassIVEiP52429
MaxQBiP52429
PaxDbiP52429
PeptideAtlasiP52429
PRIDEiP52429
ProteomicsDBi56484 [P52429-1]
74029

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		2548, 229 antibodies

The DNASU plasmid repository

More...DNASUi		8526

Genome annotation databases

EnsembliENST00000284061; ENSP00000284061; ENSG00000153933 [P52429-1]
ENST00000572810; ENSP00000459295; ENSG00000153933 [P52429-2]
GeneIDi8526
KEGGihsa:8526
UCSCiuc002iur.4, human [P52429-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		8526
DisGeNETi8526

GeneCards: human genes, protein and diseases

More...GeneCardsi		DGKE
GeneReviewsiDGKE
HGNCiHGNC:2852, DGKE
HPAiENSG00000153933, Low tissue specificity
MalaCardsiDGKE
MIMi601440, gene
615008, phenotype
neXtProtiNX_P52429
OpenTargetsiENSG00000153933
Orphaneti357008, Hemolytic uremic syndrome with DGKE deficiency
329903, Immunoglobulin-mediated membranoproliferative glomerulonephritis
PharmGKBiPA27313
VEuPathDBiHostDB:ENSG00000153933.9

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG1169, Eukaryota
GeneTreeiENSGT00940000158604
HOGENOMiCLU_1517427_0_0_1
InParanoidiP52429
OMAiWVLNTIY
OrthoDBi1275907at2759
PhylomeDBiP52429
TreeFamiTF313104

Enzyme and pathway databases

UniPathwayiUPA00230
BRENDAi2.7.1.107, 2681
PathwayCommonsiP52429
ReactomeiR-HSA-114508, Effects of PIP2 hydrolysis

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		8526, 7 hits in 873 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		DGKE, human

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		8526
PharosiP52429, Tbio

Protein Ontology

More...PROi		PR:P52429
RNActiP52429, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000153933, Expressed in middle temporal gyrus and 207 other tissues
ExpressionAtlasiP52429, baseline and differential
GenevisibleiP52429, HS

Family and domain databases

CDDicd00029, C1, 2 hits
Gene3Di3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438, ATP-NAD_kinase_N
IPR037607, DGK
IPR000756, Diacylglycerol_kin_accessory
IPR001206, Diacylglycerol_kinase_cat_dom
IPR016064, NAD/diacylglycerol_kinase_sf
IPR002219, PE/DAG-bd
PANTHERiPTHR11255, PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130, C1_1, 1 hit
PF00609, DAGK_acc, 1 hit
PF00781, DAGK_cat, 1 hit
SMARTiView protein in SMART
SM00109, C1, 2 hits
SM00045, DAGKa, 1 hit
SM00046, DAGKc, 1 hit
SUPFAMiSSF111331, SSF111331, 1 hit
PROSITEiView protein in PROSITE
PS50146, DAGK, 1 hit
PS00479, ZF_DAG_PE_1, 2 hits
PS50081, ZF_DAG_PE_2, 2 hits

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDGKE_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P52429
Secondary accession number(s): Q8TBM4, Q9UKQ3
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 10, 2021
This is version 183 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Human variants curated from literature reports
    Index of human variants curated from literature reports
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families
  5. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  6. Human entries with genetic variants
    List of human entries with genetic variants
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