UniProtKB - P52296 (IMB1_RAT)
Importin subunit beta-1
Kpnb1
Functioni
Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Imports SNAI1 and PRKCI into the nucleus (By similarity).
By similarityGO - Molecular functioni
- enzyme binding Source: RGD
- Hsp90 protein binding Source: RGD
- importin-alpha family protein binding Source: RGD
- kinesin binding Source: RGD
- nuclear import signal receptor activity Source: RGD
- nuclear localization sequence binding Source: GO_Central
- protein-containing complex binding Source: RGD
- protein domain specific binding Source: RGD
- small GTPase binding Source: InterPro
GO - Biological processi
- astral microtubule organization Source: RGD
- establishment of mitotic spindle localization Source: RGD
- establishment of protein localization Source: RGD
- mitotic chromosome movement towards spindle pole Source: RGD
- mitotic metaphase plate congression Source: RGD
- mitotic spindle assembly Source: RGD
- protein import into nucleus Source: UniProtKB
- Ran protein signal transduction Source: RGD
- ribosomal protein import into nucleus Source: RGD
- RNA import into nucleus Source: RGD
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-RNO-140342, Apoptosis induced DNA fragmentation R-RNO-1655829, Regulation of cholesterol biosynthesis by SREBP (SREBF) R-RNO-6798695, Neutrophil degranulation R-RNO-68616, Assembly of the ORC complex at the origin of replication |
Names & Taxonomyi
Protein namesi | Recommended name: Importin subunit beta-1Alternative name(s): Karyopherin subunit beta-1 Nuclear factor p97 Pore targeting complex 97 kDa subunit Short name: PTAC97 |
Gene namesi | Name:Kpnb1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2909, Kpnb1 |
Subcellular locationi
Nucleus
- Nucleus envelope 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum tubular network Source: RGD
Nucleus
- nuclear envelope Source: RGD
- nuclear pore Source: RGD
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- cytoplasmic stress granule Source: RGD
- NLS-dependent protein nuclear import complex Source: RGD
- protein-containing complex Source: RGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120747 | 1 – 875 | Importin subunit beta-1Add BLAST | 875 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 12 | PhosphoserineBy similarity | 1 | |
Modified residuei | 210 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 834 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 866 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, ADP-ribosylation, PhosphoproteinProteomic databases
jPOSTi | P52296 |
PaxDbi | P52296 |
PeptideAtlasi | P52296 |
PRIDEi | P52296 |
PTM databases
iPTMneti | P52296 |
PhosphoSitePlusi | P52296 |
Interactioni
Subunit structurei
Forms a complex with an importin alpha subunit.
Forms a heterodimer with IPO7.
Interacts with IPO7, SNUPN, RPL23A and XPO1. The KPNB1/IPO7 heterodimer interacts with H1 histone.
Interacts with H2A, H2B, H3 and H4 histones (By similarity).
Component of an import snRNP complex composed of KPNB1, SNUPN, SMN1 and ZNF259.
Component of a nuclear export receptor complex composed of KPNB1, Ran, SNUPN and XPO1.
Interacts with SRY.
Interacts with PRKCI/atypical protein kinase C iota (By similarity).
Interacts with KPNA7.
Interacts with SNAI1 (via zinc fingers) and SNAI2 (via zinc fingers) (By similarity).
Interacts with SLC35G1 and STIM1 (By similarity).
Interacts with DCAF8 (By similarity).
Interacts with RAN (By similarity).
Interacts with NUMA1 (via C-terminus); this interaction is inhibited by RanGTP (By similarity).
By similarityGO - Molecular functioni
- enzyme binding Source: RGD
- Hsp90 protein binding Source: RGD
- importin-alpha family protein binding Source: RGD
- kinesin binding Source: RGD
- protein domain specific binding Source: RGD
- small GTPase binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 247024, 5 interactors |
CORUMi | P52296 |
DIPi | DIP-37024N |
IntActi | P52296, 6 interactors |
MINTi | P52296 |
STRINGi | 10116.ENSRNOP00000012376 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 2 – 31 | HEAT 1By similarityAdd BLAST | 30 | |
Domaini | 21 – 100 | Importin N-terminalPROSITE-ProRule annotationAdd BLAST | 80 | |
Repeati | 33 – 64 | HEAT 2By similarityAdd BLAST | 32 | |
Repeati | 84 – 122 | HEAT 3By similarityAdd BLAST | 39 | |
Repeati | 128 – 159 | HEAT 4By similarityAdd BLAST | 32 | |
Repeati | 169 – 201 | HEAT 5By similarityAdd BLAST | 33 | |
Repeati | 211 – 246 | HEAT 6By similarityAdd BLAST | 36 | |
Repeati | 252 – 301 | HEAT 7By similarityAdd BLAST | 50 | |
Repeati | 313 – 359 | HEAT 8By similarityAdd BLAST | 47 | |
Repeati | 363 – 393 | HEAT 9By similarityAdd BLAST | 31 | |
Repeati | 401 – 437 | HEAT 10By similarityAdd BLAST | 37 | |
Repeati | 448 – 484 | HEAT 11By similarityAdd BLAST | 37 | |
Repeati | 499 – 536 | HEAT 12By similarityAdd BLAST | 38 | |
Repeati | 543 – 591 | HEAT 13By similarityAdd BLAST | 49 | |
Repeati | 599 – 638 | HEAT 14By similarityAdd BLAST | 40 | |
Repeati | 643 – 680 | HEAT 15By similarityAdd BLAST | 38 | |
Repeati | 685 – 723 | HEAT 16By similarityAdd BLAST | 39 | |
Repeati | 731 – 775 | HEAT 17By similarityAdd BLAST | 45 | |
Repeati | 785 – 828 | HEAT 18By similarityAdd BLAST | 44 | |
Repeati | 830 – 872 | HEAT 19By similarityAdd BLAST | 43 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 328 – 341 | IAB-bindingAdd BLAST | 14 | |
Regioni | 333 – 418 | Ran-GTP bindingBy similarityAdd BLAST | 86 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1241, Eukaryota |
InParanoidi | P52296 |
OrthoDBi | 769199at2759 |
PhylomeDBi | P52296 |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR000225, Armadillo IPR021133, HEAT_type_2 IPR001494, Importin-beta_N IPR040122, Importin_beta |
PANTHERi | PTHR10527, PTHR10527, 1 hit |
Pfami | View protein in Pfam PF03810, IBN_N, 1 hit |
SMARTi | View protein in SMART SM00185, ARM, 3 hits SM00913, IBN_N, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50077, HEAT_REPEAT, 1 hit PS50166, IMPORTIN_B_NT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MELITILEKT VSPDRLELEA AQKFLERAAV ENLPTFLVEL SRVLANPGNS
60 70 80 90 100
QVARVAAGLQ IRLLTSKDPD IKAQYQQRWL AIDANARREV KNYVLQTLGT
110 120 130 140 150
ETYRPSSASQ CVAGIACAEI PVSQWPELIP QLVANVTNPN STEHMKESTL
160 170 180 190 200
EAIGYICQDI DPEQLQDKSN EILTAIIQGM RKEEPSNNVK LAATNALLNS
210 220 230 240 250
LEFTKANFDK ESERHFIMQV VCEATQCPDT RVRVAALQNL VKIMSLYYQY
260 270 280 290 300
METYMGPALF AITIEAMKSD IDEVALQGIE FWSNVCDEEM DLAIEASEAA
310 320 330 340 350
EQGRPPEHTS KFYAKGALQY LVPILTQTLT KQDENDDDDD WNPCKAAGVC
360 370 380 390 400
LMLLSTCCED DIVPHVLPFI KEHIKNPDWR YRDAAVMAFG SILEGPEPNQ
410 420 430 440 450
LKPLVIQAMP TLIELMKDPS VVVRDTTAWT VGRICELLPE AAINDVYLAP
460 470 480 490 500
LLQCLIEGLS AEPRVASNVC WAFSSLAEAA YEAADVADDQ EEPATYCLSS
510 520 530 540 550
SFELIVQKLL ETTDRPDGHQ NNLRSSAYES LMEIVKNSAK DCYPAVQKTT
560 570 580 590 600
LVIMERLQQV LQMESHIQST SDRIQFNDLQ SLLCATLQNV LWKVQHQDAL
610 620 630 640 650
QISDVVMASL LRMFQSTAGS GGVQEDALMA VSTLVEVLGG EFLKYMEAFK
660 670 680 690 700
PFLGIGLKNY AECQVCLAAV GLVGDLCRAL QSNILPFCDE VMQLLLENLG
710 720 730 740 750
NENVHRSVKP QILSVFGDIT LAIGGEFKKY LEVVLNTLQQ ASQAQVDKSD
760 770 780 790 800
FDMVDYLNEL RESCLEAYTG IVQGLKGDQE NVHPDVMLVQ PRVEFILSFI
810 820 830 840 850
DHIAGDEDHT DGVVACAAGL IGDLCTAFGK DVLKLVEARP MIHELLTEGR
860 870
RSKTNKAKTL ATWATKELRK LKNQA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF2Z3Q8 | F2Z3Q8_RAT | Importin subunit beta-1 | Kpnb1 | 876 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L38644 mRNA Translation: AAC42047.1 |
PIRi | I59350 |
RefSeqi | NP_058759.1, NM_017063.1 |
Genome annotation databases
GeneIDi | 24917 |
KEGGi | rno:24917 |
UCSCi | RGD:2909, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L38644 mRNA Translation: AAC42047.1 |
PIRi | I59350 |
RefSeqi | NP_058759.1, NM_017063.1 |
3D structure databases
AlphaFoldDBi | P52296 |
SMRi | P52296 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 247024, 5 interactors |
CORUMi | P52296 |
DIPi | DIP-37024N |
IntActi | P52296, 6 interactors |
MINTi | P52296 |
STRINGi | 10116.ENSRNOP00000012376 |
PTM databases
iPTMneti | P52296 |
PhosphoSitePlusi | P52296 |
Proteomic databases
jPOSTi | P52296 |
PaxDbi | P52296 |
PeptideAtlasi | P52296 |
PRIDEi | P52296 |
Protocols and materials databases
DNASUi | 24917 |
Genome annotation databases
GeneIDi | 24917 |
KEGGi | rno:24917 |
UCSCi | RGD:2909, rat |
Organism-specific databases
CTDi | 3837 |
RGDi | 2909, Kpnb1 |
Phylogenomic databases
eggNOGi | KOG1241, Eukaryota |
InParanoidi | P52296 |
OrthoDBi | 769199at2759 |
PhylomeDBi | P52296 |
Enzyme and pathway databases
Reactomei | R-RNO-140342, Apoptosis induced DNA fragmentation R-RNO-1655829, Regulation of cholesterol biosynthesis by SREBP (SREBF) R-RNO-6798695, Neutrophil degranulation R-RNO-68616, Assembly of the ORC complex at the origin of replication |
Miscellaneous databases
PROi | PR:P52296 |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR000225, Armadillo IPR021133, HEAT_type_2 IPR001494, Importin-beta_N IPR040122, Importin_beta |
PANTHERi | PTHR10527, PTHR10527, 1 hit |
Pfami | View protein in Pfam PF03810, IBN_N, 1 hit |
SMARTi | View protein in SMART SM00185, ARM, 3 hits SM00913, IBN_N, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50077, HEAT_REPEAT, 1 hit PS50166, IMPORTIN_B_NT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IMB1_RAT | |
Accessioni | P52296Primary (citable) accession number: P52296 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families