UniProtKB - P52294 (IMA5_HUMAN)
Importin subunit alpha-5
KPNA1
Functioni
GO - Molecular functioni
- nuclear import signal receptor activity Source: GO_Central
- nuclear localization sequence binding Source: GO_Central
GO - Biological processi
- apoptotic DNA fragmentation Source: Reactome
- intracellular transport of virus Source: Reactome
- modulation by virus of host cellular process Source: Reactome
- NLS-bearing protein import into nucleus Source: GO_Central
- postsynapse to nucleus signaling pathway Source: GO_Central
- regulation of DNA recombination Source: ProtInc
Keywordsi
Biological process | Host-virus interaction, Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | P52294 |
Reactomei | R-HSA-1169408, ISG15 antiviral mechanism R-HSA-140342, Apoptosis induced DNA fragmentation R-HSA-162592, Integration of provirus R-HSA-168271, Transport of Ribonucleoproteins into the Host Nucleus R-HSA-168276, NS1 Mediated Effects on Host Pathways R-HSA-180910, Vpr-mediated nuclear import of PICs R-HSA-9636249, Inhibition of nitric oxide production |
SignaLinki | P52294 |
SIGNORi | P52294 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Importin subunit alpha-5Alternative name(s): Karyopherin subunit alpha-1 Nucleoprotein interactor 1 Short name: NPI-1 RAG cohort protein 2 SRP1-beta Cleaved into the following chain: |
Gene namesi | Name:KPNA1 Synonyms:RCH2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000114030.12 |
HGNCi | HGNC:6394, KPNA1 |
MIMi | 600686, gene |
neXtProti | NX_P52294 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear pore Source: ProtInc
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: ProtInc
- dendrite Source: UniProtKB
- glutamatergic synapse Source: Ensembl
- host cell Source: GOC
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 3836 |
OpenTargetsi | ENSG00000114030 |
PharmGKBi | PA30185 |
Miscellaneous databases
Pharosi | P52294, Tbio |
Polymorphism and mutation databases
BioMutai | KPNA1 |
DMDMi | 296439328 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120719 | 1 – 538 | Importin subunit alpha-5Add BLAST | 538 | |
Initiator methioninei | Removed; alternateCombined sources | |||
ChainiPRO_0000424491 | 2 – 538 | Importin subunit alpha-5, N-terminally processedAdd BLAST | 537 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 2 | N-acetylthreonine; in Importin subunit alpha-5, N-terminally processedCombined sources | 1 | |
Modified residuei | 3 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 63 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P52294 |
jPOSTi | P52294 |
MassIVEi | P52294 |
MaxQBi | P52294 |
PaxDbi | P52294 |
PeptideAtlasi | P52294 |
PRIDEi | P52294 |
ProteomicsDBi | 56476 |
PTM databases
iPTMneti | P52294 |
PhosphoSitePlusi | P52294 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000114030, Expressed in biceps brachii and 234 other tissues |
ExpressionAtlasi | P52294, baseline and differential |
Genevisiblei | P52294, HS |
Organism-specific databases
HPAi | ENSG00000114030, Tissue enhanced (skeletal) |
Interactioni
Subunit structurei
Heterodimer; with KPNB1 (PubMed:7604027).
Interacts with ANP32E (By similarity).
Interacts with ZIC3 (By similarity).
Interacts with NSMF; the interaction occurs in a calcium-independent manner after synaptic NMDA receptor stimulation and is required for nuclear import of NSMF but is competed by CABP1 (By similarity).
Interacts with APEX1 (PubMed:15942031).
Interacts with RAG1 (PubMed:8052633).
Interacts with CTNNBL1 (via its N-terminal) (PubMed:21385873).
Interacts with AICDA (via its NLS) (PubMed:21385873).
Interacts with SNAI1 (via zinc fingers) (PubMed:21454664).
Interacts with DCAF8 (PubMed:22500989).
Interacts with ITSN1 isoform 2 (PubMed:29599122).
By similarity7 Publications(Microbial infection) Interacts with human cytomegalovirus/HCMV UL84.
1 Publication(Microbial infection) Interacts with HIV-1 Vpr.
1 Publication(Microbial infection) Interacts with ebolavirus protein VP24.
2 Publications(Microbial infection) Interacts with the venezuelan equine encephalitis virus protease nsP2; this interaction probably allows the active transport of protease nsP2 into the host nucleus.
1 PublicationBinary interactionsi
Hide detailsP52294
Protein-protein interaction databases
BioGRIDi | 110034, 161 interactors |
ComplexPortali | CPX-1055, Importin complex, KPNA1 variant |
CORUMi | P52294 |
DIPi | DIP-29296N |
ELMi | P52294 |
IntActi | P52294, 105 interactors |
MINTi | P52294 |
STRINGi | 9606.ENSP00000343701 |
Miscellaneous databases
RNActi | P52294, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P52294 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P52294 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 57 | IBBPROSITE-ProRule annotationAdd BLAST | 57 | |
Repeati | 77 – 117 | ARM 1; truncatedAdd BLAST | 41 | |
Repeati | 118 – 161 | ARM 2Add BLAST | 44 | |
Repeati | 162 – 206 | ARM 3Add BLAST | 45 | |
Repeati | 207 – 245 | ARM 4Add BLAST | 39 | |
Repeati | 246 – 290 | ARM 5Add BLAST | 45 | |
Repeati | 291 – 330 | ARM 6Add BLAST | 40 | |
Repeati | 331 – 372 | ARM 7Add BLAST | 42 | |
Repeati | 373 – 412 | ARM 8Add BLAST | 40 | |
Repeati | 413 – 457 | ARM 9Add BLAST | 45 | |
Repeati | 460 – 504 | ARM 10; atypicalAdd BLAST | 45 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 149 – 241 | NLS binding site (major)By similarityAdd BLAST | 93 | |
Regioni | 245 – 437 | Binding to RAG1Add BLAST | 193 | |
Regioni | 318 – 406 | NLS binding site (minor)By similarityAdd BLAST | 89 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 42 – 51 | Nuclear localization signalBy similarity | 10 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 25 – 28 | Poly-Arg | 4 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0166, Eukaryota |
GeneTreei | ENSGT01000000214366 |
HOGENOMi | CLU_018084_6_0_1 |
InParanoidi | P52294 |
OMAi | NAYAQMI |
OrthoDBi | 1111872at2759 |
PhylomeDBi | P52294 |
TreeFami | TF354205 |
Family and domain databases
Gene3Di | 1.20.5.690, 1 hit 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR032413, Arm_3 IPR000225, Armadillo IPR002652, Importin-a_IBB IPR036975, Importin-a_IBB_sf IPR024931, Importin_alpha |
Pfami | View protein in Pfam PF00514, Arm, 8 hits PF16186, Arm_3, 1 hit PF01749, IBB, 1 hit |
PIRSFi | PIRSF005673, Importin_alpha, 1 hit |
SMARTi | View protein in SMART SM00185, ARM, 8 hits |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50176, ARM_REPEAT, 4 hits PS51214, IBB, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MTTPGKENFR LKSYKNKSLN PDEMRRRREE EGLQLRKQKR EEQLFKRRNV
60 70 80 90 100
ATAEEETEEE VMSDGGFHEA QISNMEMAPG GVITSDMIEM IFSKSPEQQL
110 120 130 140 150
SATQKFRKLL SKEPNPPIDE VISTPGVVAR FVEFLKRKEN CTLQFESAWV
160 170 180 190 200
LTNIASGNSL QTRIVIQAGA VPIFIELLSS EFEDVQEQAV WALGNIAGDS
210 220 230 240 250
TMCRDYVLDC NILPPLLQLF SKQNRLTMTR NAVWALSNLC RGKSPPPEFA
260 270 280 290 300
KVSPCLNVLS WLLFVSDTDV LADACWALSY LSDGPNDKIQ AVIDAGVCRR
310 320 330 340 350
LVELLMHNDY KVVSPALRAV GNIVTGDDIQ TQVILNCSAL QSLLHLLSSP
360 370 380 390 400
KESIKKEACW TISNITAGNR AQIQTVIDAN IFPALISILQ TAEFRTRKEA
410 420 430 440 450
AWAITNATSG GSAEQIKYLV ELGCIKPLCD LLTVMDSKIV QVALNGLENI
460 470 480 490 500
LRLGEQEAKR NGTGINPYCA LIEEAYGLDK IEFLQSHENQ EIYQKAFDLI
510 520 530
EHYFGTEDED SSIAPQVDLN QQQYIFQQCE APMEGFQL
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JYI4 | C9JYI4_HUMAN | Importin subunit alpha-5 | KPNA1 | 328 | Annotation score: | ||
C9J4U1 | C9J4U1_HUMAN | Importin subunit alpha-5 | KPNA1 | 214 | Annotation score: | ||
C9JWD9 | C9JWD9_HUMAN | Importin subunit alpha-5 | KPNA1 | 174 | Annotation score: | ||
C9J352 | C9J352_HUMAN | Importin subunit alpha-5 | KPNA1 | 169 | Annotation score: | ||
F2Z3G4 | F2Z3G4_HUMAN | Importin subunit alpha-5 | KPNA1 | 182 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 142 | T → S in AAC60648 (PubMed:7831767).Curated | 1 | |
Sequence conflicti | 169 | G → R in AAC60648 (PubMed:7831767).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_050002 | 73 | S → N4 PublicationsCorresponds to variant dbSNP:rs4678193Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S75295 mRNA Translation: AAC60648.1 BT006959 mRNA Translation: AAP35605.1 CR456743 mRNA Translation: CAG33024.1 AC083798 Genomic DNA No translation available. AC096861 Genomic DNA No translation available. CH471052 Genomic DNA Translation: EAW79482.1 CH471052 Genomic DNA Translation: EAW79483.1 BC002374 mRNA Translation: AAH02374.1 BC003009 mRNA Translation: AAH03009.1 |
CCDSi | CCDS3013.1 |
PIRi | I59931 |
RefSeqi | NP_002255.3, NM_002264.3 XP_005247494.1, XM_005247437.3 |
Genome annotation databases
Ensembli | ENST00000344337; ENSP00000343701; ENSG00000114030 |
GeneIDi | 3836 |
KEGGi | hsa:3836 |
UCSCi | uc003efe.3, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S75295 mRNA Translation: AAC60648.1 BT006959 mRNA Translation: AAP35605.1 CR456743 mRNA Translation: CAG33024.1 AC083798 Genomic DNA No translation available. AC096861 Genomic DNA No translation available. CH471052 Genomic DNA Translation: EAW79482.1 CH471052 Genomic DNA Translation: EAW79483.1 BC002374 mRNA Translation: AAH02374.1 BC003009 mRNA Translation: AAH03009.1 |
CCDSi | CCDS3013.1 |
PIRi | I59931 |
RefSeqi | NP_002255.3, NM_002264.3 XP_005247494.1, XM_005247437.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2JDQ | X-ray | 2.20 | A/B | 66-512 | [»] | |
3TJ3 | X-ray | 2.70 | A/B | 66-512 | [»] | |
4B18 | X-ray | 2.52 | A | 66-512 | [»] | |
SMRi | P52294 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110034, 161 interactors |
ComplexPortali | CPX-1055, Importin complex, KPNA1 variant |
CORUMi | P52294 |
DIPi | DIP-29296N |
ELMi | P52294 |
IntActi | P52294, 105 interactors |
MINTi | P52294 |
STRINGi | 9606.ENSP00000343701 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
PTM databases
iPTMneti | P52294 |
PhosphoSitePlusi | P52294 |
Polymorphism and mutation databases
BioMutai | KPNA1 |
DMDMi | 296439328 |
Proteomic databases
EPDi | P52294 |
jPOSTi | P52294 |
MassIVEi | P52294 |
MaxQBi | P52294 |
PaxDbi | P52294 |
PeptideAtlasi | P52294 |
PRIDEi | P52294 |
ProteomicsDBi | 56476 |
Protocols and materials databases
Antibodypediai | 32899, 374 antibodies |
DNASUi | 3836 |
Genome annotation databases
Ensembli | ENST00000344337; ENSP00000343701; ENSG00000114030 |
GeneIDi | 3836 |
KEGGi | hsa:3836 |
UCSCi | uc003efe.3, human |
Organism-specific databases
CTDi | 3836 |
DisGeNETi | 3836 |
EuPathDBi | HostDB:ENSG00000114030.12 |
GeneCardsi | KPNA1 |
HGNCi | HGNC:6394, KPNA1 |
HPAi | ENSG00000114030, Tissue enhanced (skeletal) |
MIMi | 600686, gene |
neXtProti | NX_P52294 |
OpenTargetsi | ENSG00000114030 |
PharmGKBi | PA30185 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0166, Eukaryota |
GeneTreei | ENSGT01000000214366 |
HOGENOMi | CLU_018084_6_0_1 |
InParanoidi | P52294 |
OMAi | NAYAQMI |
OrthoDBi | 1111872at2759 |
PhylomeDBi | P52294 |
TreeFami | TF354205 |
Enzyme and pathway databases
PathwayCommonsi | P52294 |
Reactomei | R-HSA-1169408, ISG15 antiviral mechanism R-HSA-140342, Apoptosis induced DNA fragmentation R-HSA-162592, Integration of provirus R-HSA-168271, Transport of Ribonucleoproteins into the Host Nucleus R-HSA-168276, NS1 Mediated Effects on Host Pathways R-HSA-180910, Vpr-mediated nuclear import of PICs R-HSA-9636249, Inhibition of nitric oxide production |
SignaLinki | P52294 |
SIGNORi | P52294 |
Miscellaneous databases
BioGRID-ORCSi | 3836, 4 hits in 849 CRISPR screens |
ChiTaRSi | KPNA1, human |
EvolutionaryTracei | P52294 |
GeneWikii | Karyopherin_alpha_1 |
GenomeRNAii | 3836 |
Pharosi | P52294, Tbio |
PROi | PR:P52294 |
RNActi | P52294, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000114030, Expressed in biceps brachii and 234 other tissues |
ExpressionAtlasi | P52294, baseline and differential |
Genevisiblei | P52294, HS |
Family and domain databases
Gene3Di | 1.20.5.690, 1 hit 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR032413, Arm_3 IPR000225, Armadillo IPR002652, Importin-a_IBB IPR036975, Importin-a_IBB_sf IPR024931, Importin_alpha |
Pfami | View protein in Pfam PF00514, Arm, 8 hits PF16186, Arm_3, 1 hit PF01749, IBB, 1 hit |
PIRSFi | PIRSF005673, Importin_alpha, 1 hit |
SMARTi | View protein in SMART SM00185, ARM, 8 hits |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50176, ARM_REPEAT, 4 hits PS51214, IBB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IMA5_HUMAN | |
Accessioni | P52294Primary (citable) accession number: P52294 Secondary accession number(s): D3DN93, Q6IBQ9, Q9BQ56 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | May 18, 2010 | |
Last modified: | December 2, 2020 | |
This is version 217 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations