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Protein

Calmegin

Gene

Clgn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions.4 Publications

GO - Molecular functioni

  • calcium ion binding Source: MGI
  • protein binding involved in protein folding Source: MGI
  • unfolded protein binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionChaperone, Developmental protein
Biological processDifferentiation, Meiosis, Spermatogenesis
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
Calmegin
Alternative name(s):
A2/6
Calnexin-T
MEG 1 antigen
Gene namesi
Name:Clgn
Synonyms:Meg1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:107472 Clgn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 471LumenalSequence analysisAdd BLAST452
Transmembranei472 – 492HelicalSequence analysisAdd BLAST21
Topological domaini493 – 611CytoplasmicSequence analysisAdd BLAST119

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Male mice show normal mating behavior and produce morphologically normal sperm, but are nearly sterile. Mutant sperm fail to adhere to the egg zona pellucida and are generally unable to penetrate the egg extracellular matrix. In addition, mutant sperm display defects in migration from the uterus into the oviduct.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000000421120 – 611CalmeginAdd BLAST592

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei128N6-acetyllysineBy similarity1
Disulfide bondi151 ↔ 185By similarity
Disulfide bondi351 ↔ 355By similarity
Modified residuei561PhosphoserineBy similarity1
Modified residuei578PhosphoserineCombined sources1
Modified residuei580PhosphoserineCombined sources1
Modified residuei582PhosphoserineCombined sources1
Modified residuei592PhosphoserineCombined sources1
Modified residuei595PhosphoserineCombined sources1
Modified residuei602PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiP52194
PaxDbiP52194
PeptideAtlasiP52194
PRIDEiP52194

PTM databases

iPTMnetiP52194
PhosphoSitePlusiP52194

Expressioni

Tissue specificityi

Detected in testis (at protein level). Detected in testis.5 Publications

Developmental stagei

Specifically expressed during male meiotic germ cell development. First detected in early pachytene spermatocytes. Expression is highest in elongating and round spermatids and decreases thereafter. Not detectable in mature spermatids.2 Publications

Gene expression databases

BgeeiENSMUSG00000002190 Expressed in 98 organ(s), highest expression level in testis
CleanExiMM_CLGN
GenevisibleiP52194 MM

Interactioni

Subunit structurei

Interacts with PDILT and PPIB (By similarity). Interacts with ADAM2. Interacts with ADAM1A, ADAM1B and ADAM3; these are protein-coding genes in mouse but may be pseudogenes in other organisms.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198750, 1 interactor
DIPiDIP-47581N
IntActiP52194, 4 interactors
MINTiP52194
STRINGi10090.ENSMUSP00000002259

Structurei

3D structure databases

ProteinModelPortaliP52194
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati267 – 2801-1Add BLAST14
Repeati284 – 2971-2Add BLAST14
Repeati303 – 3161-3Add BLAST14
Repeati322 – 3351-4Add BLAST14
Repeati339 – 3522-1Add BLAST14
Repeati356 – 3692-2Add BLAST14
Repeati370 – 3832-3Add BLAST14
Repeati384 – 3972-4Add BLAST14

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni317 – 350Interaction with PPIBBy similarityAdd BLAST34

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0675 Eukaryota
ENOG410XP7T LUCA
GeneTreeiENSGT00430000030841
HOGENOMiHOG000192436
HOVERGENiHBG005407
InParanoidiP52194
KOiK09551
OMAiKTSYTIM
OrthoDBiEOG091G04SS
TreeFamiTF300618

Family and domain databases

Gene3Di2.10.250.10, 1 hit
InterProiView protein in InterPro
IPR001580 Calret/calnex
IPR018124 Calret/calnex_CS
IPR009033 Calreticulin/calnexin_P_dom_sf
IPR013320 ConA-like_dom_sf
PANTHERiPTHR11073 PTHR11073, 1 hit
PfamiView protein in Pfam
PF00262 Calreticulin, 1 hit
PRINTSiPR00626 CALRETICULIN
SUPFAMiSSF49899 SSF49899, 1 hit
SSF63887 SSF63887, 1 hit
PROSITEiView protein in PROSITE
PS00803 CALRETICULIN_1, 1 hit
PS00804 CALRETICULIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52194-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRFQGVGLCL GLLFITVNAD FMDDGVEVED FSENSDESNI KDEPSSGTFK
60 70 80 90 100
YKTPQPIGEV YFTETFDSGN LAGWVLSKAK KDDMDSEIAI YDGRWEIEEL
110 120 130 140 150
KENQVPGDRG LVLKSKAKHH AIAAVLEKPF IFADKPLIVQ YEVNFQDGID
160 170 180 190 200
CGGAYIKLLA DTGDLILENF YDKTSYTIMF GPDKCGEDYK LHLIFRHKHP
210 220 230 240 250
KTGVFEEKHA KPPDVDLKEF FTDRKTHLYT LVMNPDDTFE VLIDQKVVNQ
260 270 280 290 300
GTLLDDVVPP INPPREIDDP SDKKPEEWDD RAKIPDPTAV RPEDWDENEP
310 320 330 340 350
AQIEDSSAVK PDGWLDDEPK FIPNPKAEKP EDWSDDMDGE WEAPHIPNPA
360 370 380 390 400
CQIGCGEWKP PMIDNPKYKG IWRPPMINNP NYQGLWSPQK IPNPDYFEDD
410 420 430 440 450
HPFLLTSFSA LGLELWSMTP DIYFDNFIIC SEKEVADQWA TDGWELKIMV
460 470 480 490 500
ANANEPGVLR QLVIAAEERP WLWLMYLVMA GLPVALVASF CWPRKVKKKY
510 520 530 540 550
EDTGPKKTEL CKLQSKAALE QEAEEEKAPE KPEDVQEEKK PGEAEVVTVE
560 570 580 590 600
KEVIGEPEEK SKEDRETLEG QEEVSKLSKS GSEDEMKDAD ESPGSGDAPL
610
KSLRKRRVRK D
Length:611
Mass (Da):69,431
Last modified:July 27, 2011 - v2
Checksum:iD70A3C418229DA00
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti76 – 79LSKA → YQS in AAA20599 (PubMed:8188695).Curated4
Sequence conflicti87E → Q in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti127E → D in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti133 – 135ADK → GAIN in AAA20599 (PubMed:8188695).Curated3
Sequence conflicti201K → Q in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti210A → P in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti291R → K in BAA03180 (PubMed:8126001).Curated1
Sequence conflicti291R → K in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti291R → K in BAA22591 (PubMed:9434179).Curated1
Sequence conflicti350A → D in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti432E → D in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti436A → P in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti440A → P in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti445E → D in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti451A → T in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti522E → D in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti549V → L in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti577 – 578LS → AE in BAB31782 (PubMed:16141072).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14117 mRNA Translation: BAA03180.1
U08373 mRNA Translation: AAA20599.1
D86323 mRNA Translation: BAA22591.1
AK019534 mRNA Translation: BAB31782.1 Sequence problems.
BC050767 mRNA Translation: AAH50767.1
CCDSiCCDS22452.1
PIRiA53418
A54086
RefSeqiNP_001316556.1, NM_001329627.1
NP_034034.2, NM_009904.4
XP_011246600.1, XM_011248298.2
UniGeneiMm.358581
Mm.426481

Genome annotation databases

EnsembliENSMUST00000002259; ENSMUSP00000002259; ENSMUSG00000002190
ENSMUST00000109831; ENSMUSP00000105457; ENSMUSG00000002190
GeneIDi12745
KEGGimmu:12745
UCSCiuc009mkd.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14117 mRNA Translation: BAA03180.1
U08373 mRNA Translation: AAA20599.1
D86323 mRNA Translation: BAA22591.1
AK019534 mRNA Translation: BAB31782.1 Sequence problems.
BC050767 mRNA Translation: AAH50767.1
CCDSiCCDS22452.1
PIRiA53418
A54086
RefSeqiNP_001316556.1, NM_001329627.1
NP_034034.2, NM_009904.4
XP_011246600.1, XM_011248298.2
UniGeneiMm.358581
Mm.426481

3D structure databases

ProteinModelPortaliP52194
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198750, 1 interactor
DIPiDIP-47581N
IntActiP52194, 4 interactors
MINTiP52194
STRINGi10090.ENSMUSP00000002259

PTM databases

iPTMnetiP52194
PhosphoSitePlusiP52194

Proteomic databases

MaxQBiP52194
PaxDbiP52194
PeptideAtlasiP52194
PRIDEiP52194

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002259; ENSMUSP00000002259; ENSMUSG00000002190
ENSMUST00000109831; ENSMUSP00000105457; ENSMUSG00000002190
GeneIDi12745
KEGGimmu:12745
UCSCiuc009mkd.1 mouse

Organism-specific databases

CTDi1047
MGIiMGI:107472 Clgn

Phylogenomic databases

eggNOGiKOG0675 Eukaryota
ENOG410XP7T LUCA
GeneTreeiENSGT00430000030841
HOGENOMiHOG000192436
HOVERGENiHBG005407
InParanoidiP52194
KOiK09551
OMAiKTSYTIM
OrthoDBiEOG091G04SS
TreeFamiTF300618

Miscellaneous databases

PROiPR:P52194
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002190 Expressed in 98 organ(s), highest expression level in testis
CleanExiMM_CLGN
GenevisibleiP52194 MM

Family and domain databases

Gene3Di2.10.250.10, 1 hit
InterProiView protein in InterPro
IPR001580 Calret/calnex
IPR018124 Calret/calnex_CS
IPR009033 Calreticulin/calnexin_P_dom_sf
IPR013320 ConA-like_dom_sf
PANTHERiPTHR11073 PTHR11073, 1 hit
PfamiView protein in Pfam
PF00262 Calreticulin, 1 hit
PRINTSiPR00626 CALRETICULIN
SUPFAMiSSF49899 SSF49899, 1 hit
SSF63887 SSF63887, 1 hit
PROSITEiView protein in PROSITE
PS00803 CALRETICULIN_1, 1 hit
PS00804 CALRETICULIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCLGN_MOUSE
AccessioniPrimary (citable) accession number: P52194
Secondary accession number(s): Q80YU3, Q9D2K5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 162 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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