UniProtKB - P52188 (KCJ12_RAT)
Protein
ATP-sensitive inward rectifier potassium channel 12
Gene
Kcnj12
Organism
Rattus norvegicus (Rat)
Status
Functioni
Inward rectifying potassium channel that is activated by phosphatidylinositol 4,5-bisphosphate and that probably participates in controlling the resting membrane potential in electrically excitable cells. Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 173 | Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity | 1 |
GO - Molecular functioni
- inward rectifier potassium channel activity Source: UniProtKB
- PDZ domain binding Source: RGD
GO - Biological processi
- potassium ion import across plasma membrane Source: GO_Central
- potassium ion transport Source: UniProtKB
- protein homotetramerization Source: UniProtKB
- regulation of ion transmembrane transport Source: GO_Central
Keywordsi
Molecular function | Ion channel, Voltage-gated channel |
Biological process | Ion transport, Potassium transport, Transport |
Ligand | Potassium |
Enzyme and pathway databases
Reactomei | R-RNO-1296041, Activation of G protein gated Potassium channels R-RNO-1296053, Classical Kir channels R-RNO-5576886, Phase 4 - resting membrane potential R-RNO-997272, Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-sensitive inward rectifier potassium channel 12Alternative name(s): Inward rectifier K(+) channel Kir2.2 Short name: IRK-2 Potassium channel, inwardly rectifying subfamily J member 12 |
Gene namesi | Name:Kcnj12 Synonyms:Irk2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621660, Kcnj12 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Other locations
- Membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: GO_Central
- T-tubule Source: RGD
Other locations
- dendrite Source: RGD
- integral component of membrane Source: UniProtKB-KW
- intrinsic component of membrane Source: UniProtKB
- neuronal cell body Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 82 | CytoplasmicBy similarityAdd BLAST | 82 | |
Transmembranei | 83 – 107 | Helical; Name=M1By similarityAdd BLAST | 25 | |
Topological domaini | 108 – 123 | ExtracellularBy similarityAdd BLAST | 16 | |
Intramembranei | 124 – 128 | By similarity | 5 | |
Intramembranei | 129 – 141 | Helical; Pore-forming; Name=H5By similarityAdd BLAST | 13 | |
Intramembranei | 142 – 151 | Pore-formingBy similarity | 10 | |
Topological domaini | 152 – 156 | ExtracellularBy similarity | 5 | |
Transmembranei | 157 – 181 | Helical; Name=M2By similarityAdd BLAST | 25 | |
Topological domaini | 182 – 427 | CytoplasmicBy similarityAdd BLAST | 246 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000154964 | 1 – 427 | ATP-sensitive inward rectifier potassium channel 12Add BLAST | 427 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 75 | S-nitrosocysteineBy similarity | 1 | |
Disulfide bondi | 155 | InterchainBy similarity |
Keywords - PTMi
Disulfide bond, S-nitrosylationProteomic databases
PaxDbi | P52188 |
PRIDEi | P52188 |
PTM databases
iPTMneti | P52188 |
PhosphoSitePlusi | P52188 |
Expressioni
Tissue specificityi
Highest level in cerebellum. Moderately found in kidney, forebrain and skeletal muscle. Not detected in uterus, liver and pancreas.1 Publication
Interactioni
Subunit structurei
Homotetramer. Forms heteromer with KCNJ4. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking.
GO - Molecular functioni
- PDZ domain binding Source: RGD
Protein-protein interaction databases
CORUMi | P52188 |
IntActi | P52188, 8 interactors |
MINTi | P52188 |
STRINGi | 10116.ENSRNOP00000054762 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 79 – 81 | Interaction with phosphatidylinositidesBy similarity | 3 | |
Motifi | 143 – 148 | Selectivity filterBy similarity | 6 | |
Motifi | 183 – 190 | Interaction with phosphatidylinositidesBy similarity | 8 | |
Motifi | 425 – 427 | PDZ-bindingSequence analysis | 3 |
Domaini
Phosphatidylinositol 4,5-bisphosphate binding to the cytoplasmic side of the channel triggers a conformation change leading to channel opening.By similarity
Sequence similaritiesi
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ12 subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3827, Eukaryota |
InParanoidi | P52188 |
PhylomeDBi | P52188 |
Family and domain databases
Gene3Di | 2.60.40.1400, 1 hit |
InterProi | View protein in InterPro IPR014756, Ig_E-set IPR041647, IRK_C IPR016449, K_chnl_inward-rec_Kir IPR003272, K_chnl_inward-rec_Kir2.2 IPR013518, K_chnl_inward-rec_Kir_cyto IPR013673, K_chnl_inward-rec_Kir_N IPR040445, Kir_TM |
PANTHERi | PTHR11767, PTHR11767, 1 hit PTHR11767:SF14, PTHR11767:SF14, 1 hit |
Pfami | View protein in Pfam PF01007, IRK, 1 hit PF17655, IRK_C, 1 hit PF08466, IRK_N, 1 hit |
PRINTSi | PR01325, KIR22CHANNEL PR01320, KIRCHANNEL |
SUPFAMi | SSF81296, SSF81296, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P52188-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTAASRANPY SIVSSEEDGL HLVTMSGANG FGNGKVHTRR RCRNRFVKKN
60 70 80 90 100
GQCNIEFANM DEKSQRYLAD MFTTCVDIRW RYMLLIFSLA FLASWLLFGI
110 120 130 140 150
IFWVIAVAHG DLEPAEGRGR TPCVLQVHGF MAAFLFSIET QTTIGYGLRC
160 170 180 190 200
VTEECPVAVF MVVAQSIVGC IIDSFMIGAI MAKMGRPKKR AQTLLFSHNA
210 220 230 240 250
VVALRDGKLC LMWRVGNLRK SHIVEAHVRA QLIKPRVTEE GEYIPLDQID
260 270 280 290 300
IDVGFDKGLD RIFLVSPITI LHEIDEASPL FGISRQDLET DDFEIVVILE
310 320 330 340 350
GMVEATAMTT QARSSYLANE ILWGHRFEPV LFEEKNQYKI DYSHFHKTYE
360 370 380 390 400
VPSTPRCSAK DLVENKFLLP SANSFCYENE LAFLSRDEED EVATDRDGRS
410 420
PQPEHDFDRL QASSGALERP YRRESEI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ6U7S0 | Q6U7S0_RAT | ATP-sensitive inward rectifier pota... | Kcnj12 | 427 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X78461 mRNA Translation: CAA55216.1 |
PIRi | S43667 |
Genome annotation databases
UCSCi | RGD:621660, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X78461 mRNA Translation: CAA55216.1 |
PIRi | S43667 |
3D structure databases
SMRi | P52188 |
ModBasei | Search... |
Protein-protein interaction databases
CORUMi | P52188 |
IntActi | P52188, 8 interactors |
MINTi | P52188 |
STRINGi | 10116.ENSRNOP00000054762 |
Chemistry databases
GuidetoPHARMACOLOGYi | 431 |
PTM databases
iPTMneti | P52188 |
PhosphoSitePlusi | P52188 |
Proteomic databases
PaxDbi | P52188 |
PRIDEi | P52188 |
Protocols and materials databases
ABCDi | P52188, 1 sequenced antibody |
Genome annotation databases
UCSCi | RGD:621660, rat |
Organism-specific databases
RGDi | 621660, Kcnj12 |
Phylogenomic databases
eggNOGi | KOG3827, Eukaryota |
InParanoidi | P52188 |
PhylomeDBi | P52188 |
Enzyme and pathway databases
Reactomei | R-RNO-1296041, Activation of G protein gated Potassium channels R-RNO-1296053, Classical Kir channels R-RNO-5576886, Phase 4 - resting membrane potential R-RNO-997272, Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits |
Miscellaneous databases
PROi | PR:P52188 |
Family and domain databases
Gene3Di | 2.60.40.1400, 1 hit |
InterProi | View protein in InterPro IPR014756, Ig_E-set IPR041647, IRK_C IPR016449, K_chnl_inward-rec_Kir IPR003272, K_chnl_inward-rec_Kir2.2 IPR013518, K_chnl_inward-rec_Kir_cyto IPR013673, K_chnl_inward-rec_Kir_N IPR040445, Kir_TM |
PANTHERi | PTHR11767, PTHR11767, 1 hit PTHR11767:SF14, PTHR11767:SF14, 1 hit |
Pfami | View protein in Pfam PF01007, IRK, 1 hit PF17655, IRK_C, 1 hit PF08466, IRK_N, 1 hit |
PRINTSi | PR01325, KIR22CHANNEL PR01320, KIRCHANNEL |
SUPFAMi | SSF81296, SSF81296, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KCJ12_RAT | |
Accessioni | P52188Primary (citable) accession number: P52188 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families