Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Presenilin sel-12

Gene

sel-12

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors (lin-12 or glp-1). Provides the major presenilin function compared to hop-1 and spe-4. Required cell-autonomously for correct neurite connectivity of the AIY cholinergic interneurons and their correct functioning in thermotaxis. Required for mesodermal patterning of muscle function.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei226By similarity1
Active sitei364By similarity1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: InterPro
  • endopeptidase activity Source: GO_Central

GO - Biological processi

  • amyloid-beta metabolic process Source: GO_Central
  • apical protein localization Source: WormBase
  • calcium ion transport Source: GO_Central
  • cell fate specification Source: WormBase
  • detection of temperature stimulus Source: UniProtKB
  • membrane protein ectodomain proteolysis Source: GO_Central
  • nervous system development Source: UniProtKB
  • Notch receptor processing Source: GO_Central
  • Notch signaling pathway Source: UniProtKB
  • oviposition Source: WormBase
  • positive regulation of Notch signaling pathway Source: WormBase
  • post-embryonic animal organ morphogenesis Source: WormBase
  • protein processing Source: InterPro
  • regulation of transforming growth factor beta receptor signaling pathway Source: WormBase

Keywordsi

Biological processNotch signaling pathway

Enzyme and pathway databases

ReactomeiR-CEL-1251985 Nuclear signaling by ERBB4
R-CEL-6798695 Neutrophil degranulation
SignaLinkiP52166

Protein family/group databases

MEROPSiA22.009

Names & Taxonomyi

Protein namesi
Recommended name:
Presenilin sel-12
Alternative name(s):
Suppressor/enhancer of lin-12 protein 12
Gene namesi
Name:sel-12
ORF Names:F35H12.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditinaRhabditomorphaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiF35H12.3 ; CE24946 ; WBGene00004769 ; sel-12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 45CytoplasmicSequence analysisAdd BLAST45
Transmembranei46 – 66HelicalSequence analysisAdd BLAST21
Topological domaini67 – 101LumenalSequence analysisAdd BLAST35
Transmembranei102 – 122HelicalSequence analysisAdd BLAST21
Topological domaini123 – 130CytoplasmicSequence analysis8
Transmembranei131 – 151HelicalSequence analysisAdd BLAST21
Topological domaini152 – 163LumenalSequence analysisAdd BLAST12
Transmembranei164 – 184HelicalSequence analysisAdd BLAST21
Topological domaini185 – 189CytoplasmicSequence analysis5
Transmembranei190 – 210HelicalSequence analysisAdd BLAST21
Topological domaini211 – 212LumenalSequence analysis2
Transmembranei213 – 233HelicalSequence analysisAdd BLAST21
Topological domaini234 – 359CytoplasmicSequence analysisAdd BLAST126
Transmembranei360 – 380HelicalSequence analysisAdd BLAST21
Topological domaini381 – 384LumenalSequence analysis4
Transmembranei385 – 405HelicalSequence analysisAdd BLAST21
Topological domaini406 – 413CytoplasmicSequence analysis8
Intramembranei414 – 434HelicalSequence analysisAdd BLAST21
Topological domaini435 – 444CytoplasmicSequence analysis10

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi60C → S in ar131; egg-laying-defective. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739031 – 444Presenilin sel-12Add BLAST444

Proteomic databases

EPDiP52166
PaxDbiP52166

Expressioni

Tissue specificityi

Expressed in most neurons.1 Publication

Developmental stagei

Expressed both maternally and zygotically. Ubiquitously expressed throughout the development and in the adult.1 Publication

Gene expression databases

BgeeiWBGene00004769 Expressed in 5 organ(s), highest expression level in multi-cellular organism

Interactioni

Subunit structurei

Homodimer. Component of the gamma-secretase complex, a complex probably composed of the presenilin homodimer (sel-12, hop-1 or spe-4), nicastrin (aph-2), aph-1 and pen-2 (Probable). Interacts with sel-10 (PubMed:9861048).Curated1 Publication

Protein-protein interaction databases

BioGridi45394, 56 interactors
STRINGi6239.F35H12.3

Structurei

3D structure databases

ProteinModelPortaliP52166
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi410 – 412PAL3

Domaini

The PAL motif is required for normal active site conformation.By similarity

Sequence similaritiesi

Belongs to the peptidase A22A family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2736 Eukaryota
ENOG410XPZD LUCA
GeneTreeiENSGT00390000016593
HOGENOMiHOG000240228
InParanoidiP52166
KOiK04505
OMAiTLCMLVV
OrthoDBiEOG091G0C72
PhylomeDBiP52166

Family and domain databases

InterProiView protein in InterPro
IPR001686 Pept_A22A_Ceel
IPR001108 Peptidase_A22A
IPR006639 Preselin/SPP
PANTHERiPTHR10202 PTHR10202, 1 hit
PfamiView protein in Pfam
PF01080 Presenilin, 1 hit
PRINTSiPR01072 PRESENILIN
PR01075 PRESENILNSEL
SMARTiView protein in SMART
SM00730 PSN, 1 hit

Sequencei

Sequence statusi: Complete.

P52166-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPSTRRQQEG GGADAETHTV YGTNLITNRN SQEDENVVEE AELKYGASHV
60 70 80 90 100
IHLFVPVSLC MALVVFTMNT ITFYSQNNGR HLLYTPFVRE TDSIVEKGLM
110 120 130 140 150
SLGNALVMLC VVVLMTVLLI VFYKYKFYKL IHGWLIVSSF LLLFLFTTIY
160 170 180 190 200
VQEVLKSFDV SPSALLVLFG LGNYGVLGMM CIHWKGPLRL QQFYLITMSA
210 220 230 240 250
LMALVFIKYL PEWTVWFVLF VISVWDLVAV LTPKGPLRYL VETAQERNEP
260 270 280 290 300
IFPALIYSSG VIYPYVLVTA VENTTDPREP TSSDSNTSTA FPGEASCSSE
310 320 330 340 350
TPKRPKVKRI PQKVQIESNT TASTTQNSGV RVERELAAER PTVQDANFHR
360 370 380 390 400
HEEEERGVKL GLGDFIFYSV LLGKASSYFD WNTTIACYVA ILIGLCFTLV
410 420 430 440
LLAVFKRALP ALPISIFSGL IFYFCTRWII TPFVTQVSQK CLLY
Length:444
Mass (Da):50,034
Last modified:May 27, 2002 - v2
Checksum:i37ADBC124E16429C
GO

Sequence cautioni

The sequence AAA85511 differs from that shown. Reason: Frameshift at position 413.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35660 mRNA Translation: AAA85511.1 Frameshift.
AF171064 mRNA Translation: AAD50991.1
FO081301 Genomic DNA Translation: CCD70617.1
RefSeqiNP_508175.1, NM_075774.5
UniGeneiCel.19557

Genome annotation databases

EnsemblMetazoaiF35H12.3; F35H12.3; WBGene00004769
GeneIDi180441
KEGGicel:CELE_F35H12.3
UCSCiF35H12.3 c. elegans

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35660 mRNA Translation: AAA85511.1 Frameshift.
AF171064 mRNA Translation: AAD50991.1
FO081301 Genomic DNA Translation: CCD70617.1
RefSeqiNP_508175.1, NM_075774.5
UniGeneiCel.19557

3D structure databases

ProteinModelPortaliP52166
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi45394, 56 interactors
STRINGi6239.F35H12.3

Protein family/group databases

MEROPSiA22.009

Proteomic databases

EPDiP52166
PaxDbiP52166

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF35H12.3; F35H12.3; WBGene00004769
GeneIDi180441
KEGGicel:CELE_F35H12.3
UCSCiF35H12.3 c. elegans

Organism-specific databases

CTDi180441
WormBaseiF35H12.3 ; CE24946 ; WBGene00004769 ; sel-12

Phylogenomic databases

eggNOGiKOG2736 Eukaryota
ENOG410XPZD LUCA
GeneTreeiENSGT00390000016593
HOGENOMiHOG000240228
InParanoidiP52166
KOiK04505
OMAiTLCMLVV
OrthoDBiEOG091G0C72
PhylomeDBiP52166

Enzyme and pathway databases

ReactomeiR-CEL-1251985 Nuclear signaling by ERBB4
R-CEL-6798695 Neutrophil degranulation
SignaLinkiP52166

Miscellaneous databases

PROiPR:P52166

Gene expression databases

BgeeiWBGene00004769 Expressed in 5 organ(s), highest expression level in multi-cellular organism

Family and domain databases

InterProiView protein in InterPro
IPR001686 Pept_A22A_Ceel
IPR001108 Peptidase_A22A
IPR006639 Preselin/SPP
PANTHERiPTHR10202 PTHR10202, 1 hit
PfamiView protein in Pfam
PF01080 Presenilin, 1 hit
PRINTSiPR01072 PRESENILIN
PR01075 PRESENILNSEL
SMARTiView protein in SMART
SM00730 PSN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPSN_CAEEL
AccessioniPrimary (citable) accession number: P52166
Secondary accession number(s): Q20076, Q9U9C7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 27, 2002
Last modified: November 7, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again