UniProtKB - P52097 (TILS_ECOLI)
Protein
tRNA(Ile)-lysidine synthase
Gene
tilS
Organism
Escherichia coli (strain K12)
Status
Functioni
Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. This enzyme is essential for viability.
Catalytic activityi
- ATP + cytidine34 in tRNAIle2 + L-lysine = AMP + diphosphate + H+ + lysidine34 in tRNAIle21 PublicationEC:6.3.4.191 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 20 – 25 | ATPSequence analysis | 6 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- identical protein binding Source: IntAct
- tRNA(Ile)-lysidine synthase activity Source: EcoCyc
GO - Biological processi
- tRNA modification Source: EcoliWiki
- tRNA wobble base lysidine biosynthesis Source: EcoCyc
Keywordsi
Molecular function | Ligase |
Biological process | tRNA processing |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G6096-MONOMER MetaCyc:G6096-MONOMER |
BRENDAi | 6.3.4.19, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA(Ile)-lysidine synthase (EC:6.3.4.19)Alternative name(s): tRNA(Ile)-2-lysyl-cytidine synthase tRNA(Ile)-lysidine synthetase |
Gene namesi | Name:tilS Synonyms:mesJ, yaeN Ordered Locus Names:b0188, JW0183 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000181689 | 1 – 432 | tRNA(Ile)-lysidine synthaseAdd BLAST | 432 |
Proteomic databases
jPOSTi | P52097 |
PaxDbi | P52097 |
PRIDEi | P52097 |
Interactioni
Binary interactionsi
P52097
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-556745,EBI-556745 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4261685, 173 interactors 849289, 3 interactors |
DIPi | DIP-10190N |
IntActi | P52097, 17 interactors |
STRINGi | 511145.b0188 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P52097 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P52097 |
Family & Domainsi
Domaini
The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.
Sequence similaritiesi
Belongs to the tRNA(Ile)-lysidine synthase family.Curated
Phylogenomic databases
eggNOGi | COG0037, Bacteria |
HOGENOMi | CLU_018869_2_0_6 |
InParanoidi | P52097 |
PhylomeDBi | P52097 |
Family and domain databases
CDDi | cd01992, PP-ATPase, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_01161, tRNA_Ile_lys_synt, 1 hit |
InterProi | View protein in InterPro IPR012796, Lysidine-tRNA-synth_C IPR014729, Rossmann-like_a/b/a_fold IPR011063, TilS/TtcA_N IPR012094, tRNA_Ile_lys_synt IPR012795, tRNA_Ile_lys_synt_N IPR015262, tRNA_Ile_lys_synt_subst-bd |
PANTHERi | PTHR43033, PTHR43033, 1 hit |
Pfami | View protein in Pfam PF01171, ATP_bind_3, 1 hit PF09179, TilS, 1 hit PF11734, TilS_C, 1 hit |
SMARTi | View protein in SMART SM00977, TilS_C, 1 hit |
TIGRFAMsi | TIGR02433, lysidine_TilS_C, 1 hit TIGR02432, lysidine_TilS_N, 1 hit |
i Sequence
Sequence statusi: Complete.
P52097-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTLTLNRQLL TSRQILVAFS GGLDSTVLLH QLVQWRTENP GVALRAIHVH
60 70 80 90 100
HGLSANADAW VTHCENVCQQ WQVPLVVERV QLAQEGLGIE AQARQARYQA
110 120 130 140 150
FARTLLPGEV LVTAQHLDDQ CETFLLALKR GSGPAGLSAM AEVSEFAGTR
160 170 180 190 200
LIRPLLARTR GELVQWARQY DLRWIEDESN QDDSYDRNFL RLRVVPLLQQ
210 220 230 240 250
RWPHFAEATA RSAALCAEQE SLLDELLADD LAHCQSPQGT LQIVPMLAMS
260 270 280 290 300
DARRAAIIRR WLAGQNAPMP SRDALVRIWQ EVALAREDAS PCLRLGAFEI
310 320 330 340 350
RRYQSQLWWI KSVTGQSENI VPWQTWLQPL ELPAGLGSVQ LNAGGDIRPP
360 370 380 390 400
RADEAVSVRF KAPGLLHIVG RNGGRKLKKI WQELGVPPWL RDTTPLLFYG
410 420 430
ETLIAAAGVF VTQEGVAEGE NGVSFVWQKT LS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D49445 Genomic DNA Translation: BAA08428.1 Z50870 Genomic DNA Translation: CAA90751.1 U70214 Genomic DNA Translation: AAB08617.1 U00096 Genomic DNA Translation: AAC73299.1 AP009048 Genomic DNA Translation: BAA77863.1 BR000032 Genomic DNA Translation: FAA00003.1 |
PIRi | D64743 |
RefSeqi | NP_414730.1, NC_000913.3 WP_000176549.1, NZ_SSZK01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC73299; AAC73299; b0188 BAA77863; BAA77863; BAA77863 |
GeneIDi | 57731449 944889 |
KEGGi | ecj:JW0183 eco:b0188 |
PATRICi | fig|1411691.4.peg.2091 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D49445 Genomic DNA Translation: BAA08428.1 Z50870 Genomic DNA Translation: CAA90751.1 U70214 Genomic DNA Translation: AAB08617.1 U00096 Genomic DNA Translation: AAC73299.1 AP009048 Genomic DNA Translation: BAA77863.1 BR000032 Genomic DNA Translation: FAA00003.1 |
PIRi | D64743 |
RefSeqi | NP_414730.1, NC_000913.3 WP_000176549.1, NZ_SSZK01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NI5 | X-ray | 2.65 | A | 1-432 | [»] | |
SMRi | P52097 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261685, 173 interactors 849289, 3 interactors |
DIPi | DIP-10190N |
IntActi | P52097, 17 interactors |
STRINGi | 511145.b0188 |
Chemistry databases
ChEMBLi | CHEMBL3309031 |
Proteomic databases
jPOSTi | P52097 |
PaxDbi | P52097 |
PRIDEi | P52097 |
Genome annotation databases
EnsemblBacteriai | AAC73299; AAC73299; b0188 BAA77863; BAA77863; BAA77863 |
GeneIDi | 57731449 944889 |
KEGGi | ecj:JW0183 eco:b0188 |
PATRICi | fig|1411691.4.peg.2091 |
Organism-specific databases
EchoBASEi | EB3011 |
Phylogenomic databases
eggNOGi | COG0037, Bacteria |
HOGENOMi | CLU_018869_2_0_6 |
InParanoidi | P52097 |
PhylomeDBi | P52097 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6096-MONOMER MetaCyc:G6096-MONOMER |
BRENDAi | 6.3.4.19, 2026 |
Miscellaneous databases
EvolutionaryTracei | P52097 |
PROi | PR:P52097 |
Family and domain databases
CDDi | cd01992, PP-ATPase, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_01161, tRNA_Ile_lys_synt, 1 hit |
InterProi | View protein in InterPro IPR012796, Lysidine-tRNA-synth_C IPR014729, Rossmann-like_a/b/a_fold IPR011063, TilS/TtcA_N IPR012094, tRNA_Ile_lys_synt IPR012795, tRNA_Ile_lys_synt_N IPR015262, tRNA_Ile_lys_synt_subst-bd |
PANTHERi | PTHR43033, PTHR43033, 1 hit |
Pfami | View protein in Pfam PF01171, ATP_bind_3, 1 hit PF09179, TilS, 1 hit PF11734, TilS_C, 1 hit |
SMARTi | View protein in SMART SM00977, TilS_C, 1 hit |
TIGRFAMsi | TIGR02433, lysidine_TilS_C, 1 hit TIGR02432, lysidine_TilS_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TILS_ECOLI | |
Accessioni | P52097Primary (citable) accession number: P52097 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families