UniProtKB - P52074 (GLCF_ECOLI)
Protein
Glycolate oxidase iron-sulfur subunit
Gene
glcF
Organism
Escherichia coli (strain K12)
Status
Functioni
Component of a complex that catalyzes the oxidation of glycolate to glyoxylate (PubMed:4557653, PubMed:8606183). Is required for E.coli to grow on glycolate as a sole source of carbon (PubMed:8606183). Is also able to oxidize D-lactate ((R)-lactate) with a similar rate (PubMed:4557653). Does not link directly to O2, and 2,6-dichloroindophenol (DCIP) and phenazine methosulfate (PMS) can act as artificial electron acceptors in vitro, but the physiological molecule that functions as primary electron acceptor during glycolate oxidation is unknown (PubMed:4557653).2 Publications
Catalytic activityi
- EC:1.1.99.141 Publication1 Publication
Cofactori
[4Fe-4S] clusterPROSITE-ProRule annotationNote: Binds 2 [4Fe-4S] clusters.PROSITE-ProRule annotation
Activity regulationi
In vitro the glycolate oxidase activity is inhibited by the sulfhydryl inhibitors CuSO4 and PCMB, by KCN, but not by the metal complexing agent EDTA.1 Publication
Kineticsi
Parameters measured from a partially purified enzyme from extracts of glycolate grown cells.1 Publication
- KM=40 µM for glycolate1 Publication
- KM=0.7 mM for D-lactate1 Publication
pH dependencei
Optimum pH is 8.0-8.8.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 25 | Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 28 | Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 31 | Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 35 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 75 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 78 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 81 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 85 | Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- D-2-hydroxy-acid dehydrogenase activity Source: RHEA
- glycolate dehydrogenase activity Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- glycolate catabolic process Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Transport |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:MONOMER0-561 MetaCyc:MONOMER0-561 |
Names & Taxonomyi
Protein namesi | Recommended name: Glycolate oxidase iron-sulfur subunit1 Publication (EC:1.1.99.141 Publication1 Publication)Alternative name(s): Glycolate dehydrogenase subunit GlcF Glycolate oxidase subunit GlcF1 Publication |
Gene namesi | Name:glcF1 Publication Synonyms:gox, yghL Ordered Locus Names:b4467, JW5486 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication1 Publication
Note: Glycolate oxidoreductase activity was shown to be firmly associated with the cytoplasmic membranes (PubMed:2689218). And the GlcF subunit itself could only be detected in the membrane fraction (PubMed:8606183).2 Publications
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Abolishes glycolate oxidase activity. Is unable to grow on glycolate as the sole source of carbon, in contrast to wild type.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000159258 | 1 – 407 | Glycolate oxidase iron-sulfur subunitAdd BLAST | 407 |
Proteomic databases
jPOSTi | P52074 |
PaxDbi | P52074 |
PRIDEi | P52074 |
Expressioni
Inductioni
Part of the glcDEFGB operon, which is induced by growth on glycolate, under the positive control of GlcC. Also induced by growth on acetate. Expression of the glc operon is strongly dependent on the integration host factor (IHF) and is repressed by the global respiratory regulator ArcA-P.1 Publication
Interactioni
Subunit structurei
The glycolate oxidase likely consists of three subunits, GlcD, GlcE and GlcF.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262366, 14 interactors |
STRINGi | 511145.b4467 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 14 – 47 | 4Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 66 – 95 | 4Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST | 30 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG0247, Bacteria |
HOGENOMi | CLU_023081_0_0_6 |
InParanoidi | P52074 |
PhylomeDBi | P52074 |
Family and domain databases
Gene3Di | 1.10.1060.10, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR004017, Cys_rich_dom IPR012257, Glc_ox_4Fe-4S IPR009051, Helical_ferredxn |
PANTHERi | PTHR32479:SF17, PTHR32479:SF17, 1 hit |
Pfami | View protein in Pfam PF02754, CCG, 2 hits PF13183, Fer4_8, 1 hit |
PIRSFi | PIRSF000139, Glc_ox_4Fe-4S, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 2 hits PS51379, 4FE4S_FER_2, 2 hits |
i Sequence
Sequence statusi: Complete.
P52074-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQTQLTEEMR QNARALEADS ILRACVHCGF CTATCPTYQL LGDELDGPRG
60 70 80 90 100
RIYLIKQVLE GNEVTLKTQE HLDRCLTCRN CETTCPSGVR YHNLLDIGRD
110 120 130 140 150
IVEQKVKRPL PERILREGLR QVVPRPAVFR ALTQVGLVLR PFLPEQVRAK
160 170 180 190 200
LPAETVKAKP RPPLRHKRRV LMLEGCAQPT LSPNTNAATA RVLDRLGISV
210 220 230 240 250
MPANEAGCCG AVDYHLNAQE KGLARARNNI DAWWPAIEAG AEAILQTASG
260 270 280 290 300
CGAFVKEYGQ MLKNDALYAD KARQVSELAV DLVELLREEP LEKLAIRGDK
310 320 330 340 350
KLAFHCPCTL QHAQKLNGEV EKVLLRLGFT LTDVPDSHLC CGSAGTYALT
360 370 380 390 400
HPDLARQLRD NKMNALESGK PEMIVTANIG CQTHLASAGR TSVRHWIEIV
EQALEKE
Sequence cautioni
The sequence AAA69145 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L43490 Genomic DNA Translation: AAB02532.1 U28377 Genomic DNA Translation: AAA69145.1 Different initiation. U00096 Genomic DNA Translation: AAT48157.1 AP009048 Genomic DNA Translation: BAE77038.1 |
RefSeqi | WP_001194661.1, NZ_SSUV01000003.1 YP_026190.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAT48157; AAT48157; b4467 BAE77038; BAE77038; BAE77038 |
GeneIDi | 2847717 |
KEGGi | ecj:JW5486 eco:b4467 |
PATRICi | fig|1411691.4.peg.3753 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L43490 Genomic DNA Translation: AAB02532.1 U28377 Genomic DNA Translation: AAA69145.1 Different initiation. U00096 Genomic DNA Translation: AAT48157.1 AP009048 Genomic DNA Translation: BAE77038.1 |
RefSeqi | WP_001194661.1, NZ_SSUV01000003.1 YP_026190.1, NC_000913.3 |
3D structure databases
SMRi | P52074 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262366, 14 interactors |
STRINGi | 511145.b4467 |
Proteomic databases
jPOSTi | P52074 |
PaxDbi | P52074 |
PRIDEi | P52074 |
Genome annotation databases
EnsemblBacteriai | AAT48157; AAT48157; b4467 BAE77038; BAE77038; BAE77038 |
GeneIDi | 2847717 |
KEGGi | ecj:JW5486 eco:b4467 |
PATRICi | fig|1411691.4.peg.3753 |
Organism-specific databases
EchoBASEi | EB3076 |
Phylogenomic databases
eggNOGi | COG0247, Bacteria |
HOGENOMi | CLU_023081_0_0_6 |
InParanoidi | P52074 |
PhylomeDBi | P52074 |
Enzyme and pathway databases
BioCyci | EcoCyc:MONOMER0-561 MetaCyc:MONOMER0-561 |
Miscellaneous databases
PROi | PR:P52074 |
Family and domain databases
Gene3Di | 1.10.1060.10, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR004017, Cys_rich_dom IPR012257, Glc_ox_4Fe-4S IPR009051, Helical_ferredxn |
PANTHERi | PTHR32479:SF17, PTHR32479:SF17, 1 hit |
Pfami | View protein in Pfam PF02754, CCG, 2 hits PF13183, Fer4_8, 1 hit |
PIRSFi | PIRSF000139, Glc_ox_4Fe-4S, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 2 hits PS51379, 4FE4S_FER_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLCF_ECOLI | |
Accessioni | P52074Primary (citable) accession number: P52074 Secondary accession number(s): P76654, Q2M9L8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |