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UniProtKB - P52062 (HEMW_ECOLI)

Entry version 143 (07 Apr 2021)
Sequence version 1 (01 Oct 1996)
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Protein

Heme chaperone HemW

Gene

hemW

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Probably acts as a heme chaperone, transferring heme to the NarI subunit of the respiratory enzyme nitrate reductase; transfer may be stimulated by NADH. Binds one molecule of heme per monomer, possibly covalently. Heme binding is not affected by either [4Fe-4S] or S-adenosyl-L-methionine (SAM)-binding. Does not have coproporphyrinogen III dehydrogenase activity in vitro (PubMed:29282292).

Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine (Probable).

1 Publication1 Publication

Miscellaneous

Might carry two S-adenosyl-L-methionine binding sites with only one binding to the iron-sulfur cluster.1 Publication

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

[4Fe-4S] clusterPROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei10S-adenosyl-L-methionine 1By similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi16Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi20Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi23Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Binding sitei66S-adenosyl-L-methionine 1; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei99S-adenosyl-L-methionine 1By similarity1
Binding sitei126S-adenosyl-L-methionine 2By similarity1
Binding sitei138S-adenosyl-L-methionine 2By similarity1
Binding sitei163S-adenosyl-L-methionine 2By similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionChaperone
Ligand4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:G7531-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Heme chaperone HemW1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:hemW1 Publication
Synonyms:yggW
Ordered Locus Names:b2955, JW2922
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Very slightly decreased anaerobic growth in minimal medium with KNO3 and glycerol.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi16 – 23CVQKCPYC → SVQKSPYS: Loss of [4Fe-4S] cofactor, protein does not dimerize, binds heme, does not transfer heme to NarGHI. 1 Publication8

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001099561 – 378Heme chaperone HemWAdd BLAST378

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P52062

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P52062

PRoteomics IDEntifications database

More...PRIDEi		P52062

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Binding of the [4Fe-4S] cofactor promotes dimerization.

1 Publication

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4262352, 41 interactors
851766, 5 interactors

Protein interaction database and analysis system

More...IntActi		P52062, 15 interactors

STRING: functional protein association networks

More...STRINGi		511145.b2955

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P52062

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini1 – 237Radical SAM corePROSITE-ProRule annotationAdd BLAST237

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni67 – 68S-adenosyl-L-methionine 2 bindingBy similarity2

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the anaerobic coproporphyrinogen-III oxidase family. HemW subfamily.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG0635, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_027579_2_1_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P52062

Database for complete collections of gene phylogenies

More...PhylomeDBi		P52062

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.80.30.20, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR034505, Coproporphyrinogen-III_oxidase
IPR006638, Elp3/MiaB/NifB
IPR010723, HemN_C
IPR004559, HemW-like
IPR007197, rSAM
IPR023404, rSAM_horseshoe

The PANTHER Classification System

More...PANTHERi		PTHR13932, PTHR13932, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF06969, HemN_C, 1 hit
PF04055, Radical_SAM, 1 hit

Structure-Function Linkage Database

More...SFLDi		SFLDF00562, HemN-like__clustered_with_heat, 1 hit
SFLDS00029, Radical_SAM, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00729, Elp3, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00539, hemN_rel, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51918, RADICAL_SAM, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P52062-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVKLPPLSLY IHIPWCVQKC PYCDFNSHAL KGEVPHDDYV QHLLNDLDND 
        60         70         80         90        100
VAYAQGREVK TIFIGGGTPS LLSGPAMQTL LDGVRARLPL AADAEITMEA 
       110        120        130        140        150
NPGTVEADRF VDYQRAGVNR ISIGVQSFSE EKLKRLGRIH GPQEAKRAAK 
       160        170        180        190        200
LASGLGLRSF NLDLMHGLPD QSLEEALGDL RQAIELNPPH LSWYQLTIEP 
       210        220        230        240        250
NTLFGSRPPV LPDDDALWDI FEQGHQLLTA AGYQQYETSA YAKPGYQCQH 
       260        270        280        290        300
NLNYWRFGDY IGIGCGAHGK VTFPDGRILR TTKTRHPRGF MQGRYLESQR 
       310        320        330        340        350
DVEATDKPFE FFMNRFRLLE AAPRVEFIAY TGLCEDVIRP QLDEAIAQGY 
       360        370 
LTECADYWQI TEHGKLFLNS LLELFLAE
Length:378
Mass (Da):42,584
Last modified:October 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i00A115BFDE4386AF
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U28377 Genomic DNA Translation: AAA69122.1
U00096 Genomic DNA Translation: AAC75992.1
AP009048 Genomic DNA Translation: BAE77018.1

Protein sequence database of the Protein Information Resource

More...PIRi		B65081

NCBI Reference Sequences

More...RefSeqi		NP_417430.1, NC_000913.3
WP_000239943.1, NZ_SSUV01000019.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC75992; AAC75992; b2955
BAE77018; BAE77018; BAE77018

Database of genes from NCBI RefSeq genomes

More...GeneIDi		947446

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW2922
eco:b2955

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.3777

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA Translation: AAA69122.1
U00096 Genomic DNA Translation: AAC75992.1
AP009048 Genomic DNA Translation: BAE77018.1
PIRiB65081
RefSeqiNP_417430.1, NC_000913.3
WP_000239943.1, NZ_SSUV01000019.1

3D structure databases

SMRiP52062
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4262352, 41 interactors
851766, 5 interactors
IntActiP52062, 15 interactors
STRINGi511145.b2955

Proteomic databases

jPOSTiP52062
PaxDbiP52062
PRIDEiP52062

Genome annotation databases

EnsemblBacteriaiAAC75992; AAC75992; b2955
BAE77018; BAE77018; BAE77018
GeneIDi947446
KEGGiecj:JW2922
eco:b2955
PATRICifig|1411691.4.peg.3777

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB2808

Phylogenomic databases

eggNOGiCOG0635, Bacteria
HOGENOMiCLU_027579_2_1_6
InParanoidiP52062
PhylomeDBiP52062

Enzyme and pathway databases

BioCyciEcoCyc:G7531-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:P52062

Family and domain databases

Gene3Di3.80.30.20, 1 hit
InterProiView protein in InterPro
IPR034505, Coproporphyrinogen-III_oxidase
IPR006638, Elp3/MiaB/NifB
IPR010723, HemN_C
IPR004559, HemW-like
IPR007197, rSAM
IPR023404, rSAM_horseshoe
PANTHERiPTHR13932, PTHR13932, 1 hit
PfamiView protein in Pfam
PF06969, HemN_C, 1 hit
PF04055, Radical_SAM, 1 hit
SFLDiSFLDF00562, HemN-like__clustered_with_heat, 1 hit
SFLDS00029, Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729, Elp3, 1 hit
TIGRFAMsiTIGR00539, hemN_rel, 1 hit
PROSITEiView protein in PROSITE
PS51918, RADICAL_SAM, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHEMW_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P52062
Secondary accession number(s): Q2M9N8
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 7, 2021
This is version 143 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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