UniProtKB - P52062 (HEMW_ECOLI)
Protein
Heme chaperone HemW
Gene
hemW
Organism
Escherichia coli (strain K12)
Status
Functioni
Probably acts as a heme chaperone, transferring heme to the NarI subunit of the respiratory enzyme nitrate reductase; transfer may be stimulated by NADH. Binds one molecule of heme per monomer, possibly covalently. Heme binding is not affected by either [4Fe-4S] or S-adenosyl-L-methionine (SAM)-binding. Does not have coproporphyrinogen III dehydrogenase activity in vitro (PubMed:29282292). Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine (Probable).1 Publication1 Publication
Miscellaneous
Might carry two S-adenosyl-L-methionine binding sites with only one binding to the iron-sulfur cluster.1 Publication
Cofactori
[4Fe-4S] clusterPROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 10 | S-adenosyl-L-methionine 1By similarity | 1 | |
Metal bindingi | 16 | Iron-sulfur (4Fe-4S-S-AdoMet)By similarity | 1 | |
Metal bindingi | 20 | Iron-sulfur (4Fe-4S-S-AdoMet)By similarity | 1 | |
Metal bindingi | 23 | Iron-sulfur (4Fe-4S-S-AdoMet)By similarity | 1 | |
Binding sitei | 66 | S-adenosyl-L-methionine 1; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 99 | S-adenosyl-L-methionine 1By similarity | 1 | |
Binding sitei | 126 | S-adenosyl-L-methionine 2By similarity | 1 | |
Binding sitei | 138 | S-adenosyl-L-methionine 2By similarity | 1 | |
Binding sitei | 163 | S-adenosyl-L-methionine 2By similarity | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: EcoCyc
- coproporphyrinogen oxidase activity Source: InterPro
- heme binding Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- porphyrin-containing compound biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Chaperone |
Ligand | 4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:G7531-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Heme chaperone HemW1 Publication |
Gene namesi | Name:hemW1 Publication Synonyms:yggW Ordered Locus Names:b2955, JW2922 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Very slightly decreased anaerobic growth in minimal medium with KNO3 and glycerol.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 16 – 23 | CVQKCPYC → SVQKSPYS: Loss of [4Fe-4S] cofactor, protein does not dimerize, binds heme, does not transfer heme to NarGHI. 1 Publication | 8 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000109956 | 1 – 378 | Heme chaperone HemWAdd BLAST | 378 |
Proteomic databases
jPOSTi | P52062 |
PaxDbi | P52062 |
PRIDEi | P52062 |
Interactioni
Subunit structurei
Binding of the [4Fe-4S] cofactor promotes dimerization.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262352, 41 interactors 851766, 5 interactors |
IntActi | P52062, 15 interactors |
STRINGi | 511145.b2955 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 237 | Radical SAM corePROSITE-ProRule annotationAdd BLAST | 237 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 67 – 68 | S-adenosyl-L-methionine 2 bindingBy similarity | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0635, Bacteria |
HOGENOMi | CLU_027579_2_1_6 |
InParanoidi | P52062 |
PhylomeDBi | P52062 |
Family and domain databases
Gene3Di | 3.80.30.20, 1 hit |
InterProi | View protein in InterPro IPR034505, Coproporphyrinogen-III_oxidase IPR006638, Elp3/MiaB/NifB IPR010723, HemN_C IPR004559, HemW-like IPR007197, rSAM IPR023404, rSAM_horseshoe |
PANTHERi | PTHR13932, PTHR13932, 1 hit |
Pfami | View protein in Pfam PF06969, HemN_C, 1 hit PF04055, Radical_SAM, 1 hit |
SFLDi | SFLDF00562, HemN-like__clustered_with_heat, 1 hit SFLDS00029, Radical_SAM, 1 hit |
SMARTi | View protein in SMART SM00729, Elp3, 1 hit |
TIGRFAMsi | TIGR00539, hemN_rel, 1 hit |
PROSITEi | View protein in PROSITE PS51918, RADICAL_SAM, 1 hit |
i Sequence
Sequence statusi: Complete.
P52062-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVKLPPLSLY IHIPWCVQKC PYCDFNSHAL KGEVPHDDYV QHLLNDLDND
60 70 80 90 100
VAYAQGREVK TIFIGGGTPS LLSGPAMQTL LDGVRARLPL AADAEITMEA
110 120 130 140 150
NPGTVEADRF VDYQRAGVNR ISIGVQSFSE EKLKRLGRIH GPQEAKRAAK
160 170 180 190 200
LASGLGLRSF NLDLMHGLPD QSLEEALGDL RQAIELNPPH LSWYQLTIEP
210 220 230 240 250
NTLFGSRPPV LPDDDALWDI FEQGHQLLTA AGYQQYETSA YAKPGYQCQH
260 270 280 290 300
NLNYWRFGDY IGIGCGAHGK VTFPDGRILR TTKTRHPRGF MQGRYLESQR
310 320 330 340 350
DVEATDKPFE FFMNRFRLLE AAPRVEFIAY TGLCEDVIRP QLDEAIAQGY
360 370
LTECADYWQI TEHGKLFLNS LLELFLAE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28377 Genomic DNA Translation: AAA69122.1 U00096 Genomic DNA Translation: AAC75992.1 AP009048 Genomic DNA Translation: BAE77018.1 |
PIRi | B65081 |
RefSeqi | NP_417430.1, NC_000913.3 WP_000239943.1, NZ_SSUV01000019.1 |
Genome annotation databases
EnsemblBacteriai | AAC75992; AAC75992; b2955 BAE77018; BAE77018; BAE77018 |
GeneIDi | 947446 |
KEGGi | ecj:JW2922 eco:b2955 |
PATRICi | fig|1411691.4.peg.3777 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28377 Genomic DNA Translation: AAA69122.1 U00096 Genomic DNA Translation: AAC75992.1 AP009048 Genomic DNA Translation: BAE77018.1 |
PIRi | B65081 |
RefSeqi | NP_417430.1, NC_000913.3 WP_000239943.1, NZ_SSUV01000019.1 |
3D structure databases
SMRi | P52062 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262352, 41 interactors 851766, 5 interactors |
IntActi | P52062, 15 interactors |
STRINGi | 511145.b2955 |
Proteomic databases
jPOSTi | P52062 |
PaxDbi | P52062 |
PRIDEi | P52062 |
Genome annotation databases
EnsemblBacteriai | AAC75992; AAC75992; b2955 BAE77018; BAE77018; BAE77018 |
GeneIDi | 947446 |
KEGGi | ecj:JW2922 eco:b2955 |
PATRICi | fig|1411691.4.peg.3777 |
Organism-specific databases
EchoBASEi | EB2808 |
Phylogenomic databases
eggNOGi | COG0635, Bacteria |
HOGENOMi | CLU_027579_2_1_6 |
InParanoidi | P52062 |
PhylomeDBi | P52062 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7531-MONOMER |
Miscellaneous databases
PROi | PR:P52062 |
Family and domain databases
Gene3Di | 3.80.30.20, 1 hit |
InterProi | View protein in InterPro IPR034505, Coproporphyrinogen-III_oxidase IPR006638, Elp3/MiaB/NifB IPR010723, HemN_C IPR004559, HemW-like IPR007197, rSAM IPR023404, rSAM_horseshoe |
PANTHERi | PTHR13932, PTHR13932, 1 hit |
Pfami | View protein in Pfam PF06969, HemN_C, 1 hit PF04055, Radical_SAM, 1 hit |
SFLDi | SFLDF00562, HemN-like__clustered_with_heat, 1 hit SFLDS00029, Radical_SAM, 1 hit |
SMARTi | View protein in SMART SM00729, Elp3, 1 hit |
TIGRFAMsi | TIGR00539, hemN_rel, 1 hit |
PROSITEi | View protein in PROSITE PS51918, RADICAL_SAM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HEMW_ECOLI | |
Accessioni | P52062Primary (citable) accession number: P52062 Secondary accession number(s): Q2M9N8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families