UniProtKB - P52045 (SCPB_ECOLI)
Protein
Methylmalonyl-CoA decarboxylase
Gene
scpB
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the decarboxylation of (R)-methylmalonyl-CoA to propionyl-CoA. Could be part of a pathway that converts succinate to propanoate.1 Publication
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 110 | Substrate; via amide nitrogen1 Publication | 1 | |
Binding sitei | 132 | Substrate1 Publication | 1 | |
Binding sitei | 253 | Substrate1 Publication | 1 |
GO - Molecular functioni
- carboxy-lyase activity Source: UniProtKB
- enoyl-CoA hydratase activity Source: GO_Central
- methylmalonyl-CoA decarboxylase activity Source: EcoCyc
GO - Biological processi
- fatty acid beta-oxidation Source: GO_Central
Keywordsi
Molecular function | Lyase |
Enzyme and pathway databases
BioCyci | EcoCyc:G7516-MONOMER MetaCyc:G7516-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Methylmalonyl-CoA decarboxylase (EC:4.1.1.-)Short name: MMCD Alternative name(s): Transcarboxylase |
Gene namesi | Name:scpB Synonyms:mmcD, ygfG Ordered Locus Names:b2919, JW2886 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000109355 | 1 – 261 | Methylmalonyl-CoA decarboxylaseAdd BLAST | 261 |
Proteomic databases
PaxDbi | P52045 |
PRIDEi | P52045 |
Interactioni
Subunit structurei
Dimer of homotrimers.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4259438, 2 interactors |
STRINGi | 511145.b2919 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P52045 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P52045 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 64 – 68 | Substrate binding | 5 |
Sequence similaritiesi
Belongs to the enoyl-CoA hydratase/isomerase family.Curated
Phylogenomic databases
eggNOGi | COG1024, Bacteria |
HOGENOMi | CLU_009834_7_3_6 |
InParanoidi | P52045 |
PhylomeDBi | P52045 |
Family and domain databases
Gene3Di | 1.10.12.10, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR018376, Enoyl-CoA_hyd/isom_CS IPR001753, Enoyl-CoA_hydra/iso IPR014748, Enoyl-CoA_hydra_C |
Pfami | View protein in Pfam PF00378, ECH_1, 1 hit |
SUPFAMi | SSF52096, SSF52096, 1 hit |
PROSITEi | View protein in PROSITE PS00166, ENOYL_COA_HYDRATASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P52045-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSYQYVNVVT INKVAVIEFN YGRKLNALSK VFIDDLMQAL SDLNRPEIRC
60 70 80 90 100
IILRAPSGSK VFSAGHDIHE LPSGGRDPLS YDDPLRQITR MIQKFPKPII
110 120 130 140 150
SMVEGSVWGG AFEMIMSSDL IIAASTSTFS MTPVNLGVPY NLVGIHNLTR
160 170 180 190 200
DAGFHIVKEL IFTASPITAQ RALAVGILNH VVEVEELEDF TLQMAHHISE
210 220 230 240 250
KAPLAIAVIK EELRVLGEAH TMNSDEFERI QGMRRAVYDS EDYQEGMNAF
260
LEKRKPNFVG H
Sequence cautioni
The sequence AAA69086 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28377 Genomic DNA Translation: AAA69086.1 Different initiation. U00096 Genomic DNA Translation: AAC75956.2 AP009048 Genomic DNA Translation: BAE76983.1 |
RefSeqi | NP_417394.4, NC_000913.3 WP_000122080.1, NZ_STEB01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC75956; AAC75956; b2919 BAE76983; BAE76983; BAE76983 |
GeneIDi | 57730271 947408 |
KEGGi | ecj:JW2886 eco:b2919 |
PATRICi | fig|1411691.4.peg.3813 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28377 Genomic DNA Translation: AAA69086.1 Different initiation. U00096 Genomic DNA Translation: AAC75956.2 AP009048 Genomic DNA Translation: BAE76983.1 |
RefSeqi | NP_417394.4, NC_000913.3 WP_000122080.1, NZ_STEB01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EF8 | X-ray | 1.85 | A/B/C | 1-261 | [»] | |
1EF9 | X-ray | 2.70 | A | 1-261 | [»] | |
6N92 | X-ray | 1.70 | A/B/C/D/E/F | 1-261 | [»] | |
6N93 | X-ray | 1.70 | A/B/C/D/E/F | 1-261 | [»] | |
6N94 | X-ray | 1.75 | A/B/C/D/E/F | 1-261 | [»] | |
6N95 | X-ray | 1.80 | A/B/C/D/E/F | 1-261 | [»] | |
6N96 | X-ray | 1.70 | A/B/C/D/E/F | 1-261 | [»] | |
6N97 | X-ray | 1.75 | A/B/C/D/E/F | 1-261 | [»] | |
SMRi | P52045 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259438, 2 interactors |
STRINGi | 511145.b2919 |
Chemistry databases
DrugBanki | DB03117, 2-carboxypropyl-coenzyme A |
Proteomic databases
PaxDbi | P52045 |
PRIDEi | P52045 |
Genome annotation databases
EnsemblBacteriai | AAC75956; AAC75956; b2919 BAE76983; BAE76983; BAE76983 |
GeneIDi | 57730271 947408 |
KEGGi | ecj:JW2886 eco:b2919 |
PATRICi | fig|1411691.4.peg.3813 |
Organism-specific databases
EchoBASEi | EB2799 |
Phylogenomic databases
eggNOGi | COG1024, Bacteria |
HOGENOMi | CLU_009834_7_3_6 |
InParanoidi | P52045 |
PhylomeDBi | P52045 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7516-MONOMER MetaCyc:G7516-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P52045 |
PROi | PR:P52045 |
Family and domain databases
Gene3Di | 1.10.12.10, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR018376, Enoyl-CoA_hyd/isom_CS IPR001753, Enoyl-CoA_hydra/iso IPR014748, Enoyl-CoA_hydra_C |
Pfami | View protein in Pfam PF00378, ECH_1, 1 hit |
SUPFAMi | SSF52096, SSF52096, 1 hit |
PROSITEi | View protein in PROSITE PS00166, ENOYL_COA_HYDRATASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SCPB_ECOLI | |
Accessioni | P52045Primary (citable) accession number: P52045 Secondary accession number(s): P76643, Q2M9S3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families