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UniProtKB - P51996 (YPT32_YEAST)
Protein
GTP-binding protein YPT32/YPT11
Gene
YPT32
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Required for protein transport through the secretory pathway. Probably involved in regulation of secretory vesicle formation at the trans-Golgi compartment. Mediates the proper polarized localization of SEC2, a GEF for SEC4, but does not alter the exchange activity of SEC2 on SEC4. Plays a role in autophagy.
5 PublicationsMiscellaneous
Present with 4298 molecules/cell in log phase SD medium.1 Publication
Activity regulationi
Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) TRAPP complex, and inactivated by GTPase-activating protein (GAP) GYP3.2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 20 – 27 | GTPBy similarity | 8 | |
Nucleotide bindingi | 68 – 72 | GTPBy similarity | 5 | |
Nucleotide bindingi | 126 – 129 | GTPBy similarity | 4 |
GO - Molecular functioni
- GTPase activity Source: SGD
- GTP binding Source: UniProtKB-KW
GO - Biological processi
- autophagy Source: UniProtKB-KW
- early endosome to Golgi transport Source: SGD
- exocytosis Source: SGD
- protein transport Source: UniProtKB-KW
- vesicle-mediated transport Source: SGD
Keywordsi
Biological process | Autophagy, Exocytosis, Protein transport, Transport |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-5620912, Anchoring of the basal body to the plasma membrane R-SCE-8873719, RAB geranylgeranylation |
Names & Taxonomyi
Protein namesi | Recommended name: GTP-binding protein YPT32/YPT11Alternative name(s): Rab GTPase YPT32 |
Gene namesi | Name:YPT32 Synonyms:YPT11 Ordered Locus Names:YGL210W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003178, YPT32 |
VEuPathDBi | FungiDB:YGL210W |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane 1 Publication; Lipid-anchor 1 Publication
Cytosol
- cytosol Source: GOC
Endosome
- endosome Source: SGD
- recycling endosome Source: GO_Central
Golgi apparatus
- Golgi apparatus Source: SGD
- Golgi membrane Source: UniProtKB-SubCell
Mitochondrion
- mitochondrial outer membrane Source: SGD
Other locations
- cellular bud neck Source: SGD
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 27 | S → N: Binds preferentially GDP. 1 Publication | 1 | |
Mutagenesisi | 49 | E → Q: Cold sensitive. 1 Publication | 1 | |
Mutagenesisi | 72 | Q → L: Reduces GTPase activity. 1 Publication | 1 | |
Mutagenesisi | 126 | N → I: Blocks nucleotide binding. 1 Publication | 1 | |
Mutagenesisi | 141 | A → D: Loss of function at 37 degrees Celsius. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000121324 | 2 – 222 | GTP-binding protein YPT32/YPT11Add BLAST | 221 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Lipidationi | 221 | S-geranylgeranyl cysteineBy similarity | 1 | |
Lipidationi | 222 | S-geranylgeranyl cysteineBy similarity | 1 |
Keywords - PTMi
Acetylation, Lipoprotein, PrenylationProteomic databases
MaxQBi | P51996 |
PaxDbi | P51996 |
PRIDEi | P51996 |
PTM databases
iPTMneti | P51996 |
Interactioni
Subunit structurei
Interacts with YIF1, YIP3, YIP4 and YIP5.
Interacts with the GEF SEC2.
Interacts with TRS130.
4 PublicationsBinary interactionsi
P51996
With | #Exp. | IntAct |
---|---|---|
GDI1 [P39958] | 3 | EBI-29384,EBI-7517 |
GYP1 [Q08484] | 3 | EBI-29384,EBI-8005 |
YIF1 [P53845] | 2 | EBI-29384,EBI-28230 |
YIP3 [P53633] | 2 | EBI-29384,EBI-25301 |
YIP4 [P53093] | 2 | EBI-29384,EBI-24124 |
Protein-protein interaction databases
BioGRIDi | 33046, 110 interactors |
DIPi | DIP-6597N |
IntActi | P51996, 7 interactors |
MINTi | P51996 |
STRINGi | 4932.YGL210W |
Miscellaneous databases
RNActi | P51996, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P51996 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 186 – 222 | DisorderedSequence analysisAdd BLAST | 37 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 42 – 50 | Effector regionCurated | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 186 – 202 | Polar residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0087, Eukaryota |
GeneTreei | ENSGT00940000171249 |
HOGENOMi | CLU_041217_23_0_1 |
InParanoidi | P51996 |
OMAi | ITAIYQM |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P51996-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNEDYGYDY DYLFKIVLIG DSGVGKSNLL SRFTTDEFNI ESKSTIGVEF
60 70 80 90 100
ATRTIEVENK KIKAQIWDTA GQERYRAITS AYYRGAVGAL IVYDISKSSS
110 120 130 140 150
YENCNHWLTE LRENADDNVA VGLIGNKSDL AHLRAVPTDE AKNFAMENQM
160 170 180 190 200
LFTETSALNS DNVDKAFREL IVAIFQMVSK HQVDLSGSGT NNMGSNGAPK
210 220
GPTISLTPAP KEDKKKKSSN CC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72834 Genomic DNA Translation: CAA51355.1 U33754 Genomic DNA Translation: AAC49495.1 Z72732 Genomic DNA Translation: CAA96926.1 AY558506 Genomic DNA Translation: AAS56832.1 BK006941 Genomic DNA Translation: DAA07906.1 |
PIRi | S58514 |
RefSeqi | NP_011305.1, NM_001181075.1 |
Genome annotation databases
EnsemblFungii | YGL210W_mRNA; YGL210W; YGL210W |
GeneIDi | 852662 |
KEGGi | sce:YGL210W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72834 Genomic DNA Translation: CAA51355.1 U33754 Genomic DNA Translation: AAC49495.1 Z72732 Genomic DNA Translation: CAA96926.1 AY558506 Genomic DNA Translation: AAS56832.1 BK006941 Genomic DNA Translation: DAA07906.1 |
PIRi | S58514 |
RefSeqi | NP_011305.1, NM_001181075.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3RWM | X-ray | 2.00 | B | 7-188 | [»] | |
3RWO | X-ray | 1.70 | A/B | 7-188 | [»] | |
SMRi | P51996 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33046, 110 interactors |
DIPi | DIP-6597N |
IntActi | P51996, 7 interactors |
MINTi | P51996 |
STRINGi | 4932.YGL210W |
PTM databases
iPTMneti | P51996 |
Proteomic databases
MaxQBi | P51996 |
PaxDbi | P51996 |
PRIDEi | P51996 |
Genome annotation databases
EnsemblFungii | YGL210W_mRNA; YGL210W; YGL210W |
GeneIDi | 852662 |
KEGGi | sce:YGL210W |
Organism-specific databases
SGDi | S000003178, YPT32 |
VEuPathDBi | FungiDB:YGL210W |
Phylogenomic databases
eggNOGi | KOG0087, Eukaryota |
GeneTreei | ENSGT00940000171249 |
HOGENOMi | CLU_041217_23_0_1 |
InParanoidi | P51996 |
OMAi | ITAIYQM |
Enzyme and pathway databases
Reactomei | R-SCE-5620912, Anchoring of the basal body to the plasma membrane R-SCE-8873719, RAB geranylgeranylation |
Miscellaneous databases
PROi | PR:P51996 |
RNActi | P51996, protein |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | YPT32_YEAST | |
Accessioni | P51996Primary (citable) accession number: P51996 Secondary accession number(s): D6VTU5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | January 23, 2007 | |
Last modified: | September 29, 2021 | |
This is version 189 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families