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UniProtKB - P51981 (AEEP_ECOLI)
Protein
L-Ala-D/L-Glu epimerase
Gene
ycjG
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L-Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, except L-Ala-L-Arg, L-Ala-L-Lys and L-Ala-L-Pro. Is also active with L-Gly-L-Glu, L-Phe-L-Glu, and L-Ser-L-Glu, but not with L-Glu-L-Glu, L-Lys-L-Glu, L-Pro-L-Glu, L-Lys-L-Ala, or D-Ala-D-Ala.
2 PublicationsCatalytic activityi
- EC:5.1.1.201 Publication
Cofactori
Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
Kineticsi
- KM=0.13 mM for L-Ala-D-Glu (at pH 8.5)1 Publication
- KM=0.19 mM for L-Ala-D-Asp (at pH 8.5)1 Publication
- KM=0.69 mM for L-Ala-D-Met (at pH 8.5)1 Publication
- KM=1.8 mM for L-Ala-D-Gln (at pH 8.5)1 Publication
: peptidoglycan recycling Pathwayi
This protein is involved in the pathway peptidoglycan recycling, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan recycling and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 124 | SubstrateBy similarity | 1 | |
Binding sitei | 149 | SubstrateBy similarity | 1 | |
Active sitei | 151 | Proton acceptor; specific for (R)-substrate epimerizationBy similarity | 1 | |
Metal bindingi | 176 | MagnesiumBy similarity | 1 | |
Metal bindingi | 202 | MagnesiumBy similarity | 1 | |
Metal bindingi | 225 | MagnesiumBy similarity | 1 | |
Active sitei | 247 | Proton acceptor; specific for (S)-substrate epimerizationBy similarity | 1 | |
Binding sitei | 275 | Substrate; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 297 | SubstrateBy similarity | 1 | |
Binding sitei | 299 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- L-Ala-D/L-Glu epimerase activity Source: RHEA
- metal ion binding Source: UniProtKB-KW
- racemase and epimerase activity, acting on amino acids and derivatives Source: EcoCyc
GO - Biological processi
- cellular amino acid catabolic process Source: InterPro
- cell wall organization Source: UniProtKB-KW
- peptidoglycan turnover Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Isomerase |
Biological process | Cell wall biogenesis/degradation |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G6661-MONOMER |
BRENDAi | 5.1.1.20, 2026 |
SABIO-RKi | P51981 |
UniPathwayi | UPA00544 |
Names & Taxonomyi
Protein namesi | Recommended name: L-Ala-D/L-Glu epimerase (EC:5.1.1.201 Publication)Short name: AE epimerase Short name: AEE |
Gene namesi | Name:ycjG Synonyms:ycjH Ordered Locus Names:b1325, JW1318 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000171261 | 1 – 321 | L-Ala-D/L-Glu epimeraseAdd BLAST | 321 |
Proteomic databases
jPOSTi | P51981 |
PaxDbi | P51981 |
PRIDEi | P51981 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4260152, 370 interactors |
IntActi | P51981, 1 interactor |
STRINGi | 511145.b1325 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P51981 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P51981 |
Family & Domainsi
Sequence similaritiesi
Belongs to the mandelate racemase/muconate lactonizing enzyme family.Curated
Phylogenomic databases
eggNOGi | COG4948, Bacteria |
HOGENOMi | CLU_030273_4_3_6 |
InParanoidi | P51981 |
PhylomeDBi | P51981 |
Family and domain databases
CDDi | cd03319, L-Ala-DL-Glu_epimerase, 1 hit |
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
InterProi | View protein in InterPro IPR034603, Dipeptide_epimerase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR029065, Enolase_C-like IPR018110, Mandel_Rmase/mucon_lact_enz_CS IPR013342, Mandelate_racemase_C IPR034593, Mandelate_racemase_DgoD-like IPR013341, Mandelate_racemase_N_dom |
PANTHERi | PTHR48080, PTHR48080, 2 hits |
Pfami | View protein in Pfam PF13378, MR_MLE_C, 1 hit PF02746, MR_MLE_N, 1 hit |
SFLDi | SFLDF00010, dipeptide_epimerase, 1 hit |
SMARTi | View protein in SMART SM00922, MR_MLE, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
PROSITEi | View protein in PROSITE PS00908, MR_MLE_1, 1 hit PS00909, MR_MLE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P51981-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRTVKVFEEA WPLHTPFVIA RGSRSEARVV VVELEEEGIK GTGECTPYPR
60 70 80 90 100
YGESDASVMA QIMSVVPQLE KGLTREELQK ILPAGAARNA LDCALWDLAA
110 120 130 140 150
RRQQQSLADL IGITLPETVI TAQTVVIGTP DQMANSASTL WQAGAKLLKV
160 170 180 190 200
KLDNHLISER MVAIRTAVPD ATLIVDANES WRAEGLAARC QLLADLGVAM
210 220 230 240 250
LEQPLPAQDD AALENFIHPL PICADESCHT RSNLKALKGR YEMVNIKLDK
260 270 280 290 300
TGGLTEALAL ATEARAQGFS LMLGCMLCTS RAISAALPLV PQVSFADLDG
310 320
PTWLAVDVEP ALQFTTGELH L
Sequence cautioni
The sequence U33213 differs from that shown. Reason: Frameshift.Curated
The sequence U33213 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 236 | A → R in U33213 (PubMed:7499381).Curated | 1 | |
Sequence conflicti | 244 | V → I in U33213 (PubMed:7499381).Curated | 1 | |
Sequence conflicti | 257 | Missing in U33213 (PubMed:7499381).Curated | 1 | |
Sequence conflicti | 259 | A → G in U33213 (PubMed:7499381).Curated | 1 | |
Sequence conflicti | 301 | P → S in U33213 (PubMed:7499381).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U33213 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC74407.2 AP009048 Genomic DNA Translation: BAA14907.1 |
PIRi | H64881 |
RefSeqi | NP_415841.4, NC_000913.3 WP_001261211.1, NZ_SSZK01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC74407; AAC74407; b1325 BAA14907; BAA14907; BAA14907 |
GeneIDi | 946013 |
KEGGi | ecj:JW1318 eco:b1325 |
PATRICi | fig|1411691.4.peg.953 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U33213 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC74407.2 AP009048 Genomic DNA Translation: BAA14907.1 |
PIRi | H64881 |
RefSeqi | NP_415841.4, NC_000913.3 WP_001261211.1, NZ_SSZK01000012.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JPD | X-ray | 2.60 | X | 1-321 | [»] | |
SMRi | P51981 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260152, 370 interactors |
IntActi | P51981, 1 interactor |
STRINGi | 511145.b1325 |
Proteomic databases
jPOSTi | P51981 |
PaxDbi | P51981 |
PRIDEi | P51981 |
Genome annotation databases
EnsemblBacteriai | AAC74407; AAC74407; b1325 BAA14907; BAA14907; BAA14907 |
GeneIDi | 946013 |
KEGGi | ecj:JW1318 eco:b1325 |
PATRICi | fig|1411691.4.peg.953 |
Organism-specific databases
EchoBASEi | EB3018 |
Phylogenomic databases
eggNOGi | COG4948, Bacteria |
HOGENOMi | CLU_030273_4_3_6 |
InParanoidi | P51981 |
PhylomeDBi | P51981 |
Enzyme and pathway databases
UniPathwayi | UPA00544 |
BioCyci | EcoCyc:G6661-MONOMER |
BRENDAi | 5.1.1.20, 2026 |
SABIO-RKi | P51981 |
Miscellaneous databases
EvolutionaryTracei | P51981 |
PROi | PR:P51981 |
Family and domain databases
CDDi | cd03319, L-Ala-DL-Glu_epimerase, 1 hit |
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
InterProi | View protein in InterPro IPR034603, Dipeptide_epimerase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR029065, Enolase_C-like IPR018110, Mandel_Rmase/mucon_lact_enz_CS IPR013342, Mandelate_racemase_C IPR034593, Mandelate_racemase_DgoD-like IPR013341, Mandelate_racemase_N_dom |
PANTHERi | PTHR48080, PTHR48080, 2 hits |
Pfami | View protein in Pfam PF13378, MR_MLE_C, 1 hit PF02746, MR_MLE_N, 1 hit |
SFLDi | SFLDF00010, dipeptide_epimerase, 1 hit |
SMARTi | View protein in SMART SM00922, MR_MLE, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
PROSITEi | View protein in PROSITE PS00908, MR_MLE_1, 1 hit PS00909, MR_MLE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AEEP_ECOLI | |
Accessioni | P51981Primary (citable) accession number: P51981 Secondary accession number(s): P51982, P76048, P77345 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 160 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families