UniProtKB - P51960 (MYBA_MOUSE)
Myb-related protein A
Mybl1
Functioni
Transcription factor that specifically recognizes the sequence 5'-YAAC[GT]G-3' (PubMed:7813437, PubMed:23523368).
Acts as a master regulator of male meiosis by promoting expression of piRNAs: activates expression of both piRNA precursor RNAs and expression of protein-coding genes involved in piRNA metabolism, such as PIWIL1 (PubMed:21750041, PubMed:23523368).
The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons, which is essential for the germline integrity (PubMed:23523368).
Transcriptional activator of SOX30 (PubMed:29848638).
4 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 58 – 81 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 24 | |
DNA bindingi | 110 – 133 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 24 | |
DNA bindingi | 161 – 184 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- male meiosis I Source: UniProtKB
- mitotic cell cycle Source: GO_Central
- negative regulation of transposition Source: UniProtKB
- piRNA biosynthetic process Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of transcription, DNA-templated Source: GO_Central
- spermatogenesis Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Differentiation, Spermatogenesis, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Myb-related protein A1 PublicationShort name: A-Myb1 Publication Alternative name(s): Myb-like protein 1 |
Gene namesi | Name:Mybl1 Synonyms:Amyb |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:99925, Mybl1 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 213 | A → E in Repro9; male sterility due to defects in spermatogenesis. Impaired piRNA biogenesis. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000197055 | 1 – 751 | Myb-related protein AAdd BLAST | 751 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 199 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 393 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 591 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 601 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Acetylation, Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | P51960 |
PRIDEi | P51960 |
ProteomicsDBi | 286091 [P51960-1] 286092 [P51960-2] |
PTM databases
iPTMneti | P51960 |
PhosphoSitePlusi | P51960 |
Expressioni
Tissue specificityi
Developmental stagei
Interactioni
Subunit structurei
Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2 (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000086034 |
Miscellaneous databases
RNActi | P51960, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 30 – 81 | HTH myb-type 1PROSITE-ProRule annotationAdd BLAST | 52 | |
Domaini | 82 – 137 | HTH myb-type 2PROSITE-ProRule annotationAdd BLAST | 56 | |
Domaini | 138 – 188 | HTH myb-type 3PROSITE-ProRule annotationAdd BLAST | 51 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 22 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 230 – 294 | Transcriptional activation domainBy similarityAdd BLAST | 65 | |
Regioni | 297 – 552 | Negative regulatory domainBy similarityAdd BLAST | 256 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0048, Eukaryota |
InParanoidi | P51960 |
OrthoDBi | 219341at2759 |
PhylomeDBi | P51960 |
TreeFami | TF326257 |
Family and domain databases
CDDi | cd00167, SANT, 3 hits |
InterProi | View protein in InterPro IPR015395, C-myb_C IPR009057, Homeobox-like_sf IPR017930, Myb_dom IPR028317, MYBL1 IPR001005, SANT/Myb IPR012642, Tscrpt_reg_Wos2-domain |
PANTHERi | PTHR45614:SF9, PTHR45614:SF9, 1 hit |
Pfami | View protein in Pfam PF09316, Cmyb_C, 1 hit PF07988, LMSTEN, 1 hit PF00249, Myb_DNA-binding, 1 hit |
SMARTi | View protein in SMART SM00717, SANT, 3 hits |
SUPFAMi | SSF46689, SSF46689, 2 hits |
PROSITEi | View protein in PROSITE PS51294, HTH_MYB, 3 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAKRSRSEDE DDDLQYADHD YEVPQQKGLK KLWNRVKWTR DEDDKLKKLV
60 70 80 90 100
EQHGTDDWTL IASHLQNRSD FQCQHRWQKV LNPELIKGPW TKEEDQRVIE
110 120 130 140 150
LVQKYGPKRW SLIAKHLKGR IGKQCRERWH NHLNPEVKKS SWTEEEDRII
160 170 180 190 200
YEAHKRLGNR WAEIAKLLPG RTDNSIKNHW NSTMRRKVEQ EGYLQDGIKS
210 220 230 240 250
ERSSSKLQHK PCATMDHLQT QNQFYIPVQI PGYQYVSPDG NCVEHVQTSA
260 270 280 290 300
FIQQPFVDED PDKEKKIKEL ELLLMSAENE VRRKRLPPQP GSFSSWSGSF
310 320 330 340 350
LMDDSMSNTL NNLEEHTTEF YSMDENQTVS AQQNSPTKFL AVEANAVLSS
360 370 380 390 400
LQTIPEFPRT LELIESDPVA WSDVTSFDLS DAAASPVKST PVKLMRIQHN
410 420 430 440 450
EGAMECQFNV SLVLEGKKNS RNGGDSEAIP LTSPNVVKFS TPPTISRKKK
460 470 480 490 500
RIRVGQSAGS ELGSASLSEV GNRRIKHTPV KTLPFSPSQF FNTCPGNEQL
510 520 530 540 550
NIENPSFTST PICGQKVLIT TPLQKEATPK DQKENVGFRT PTIRRSILGT
560 570 580 590 600
TPRTPTPFKN ALAAQEKKYG PLKIVSQPLA FLEEDIREVL KEETGTDIFL
610 620 630 640 650
KEEDEPAYKS CKQEHSASVK KVRKSLALES WDKEEPGTQL LTEDISDMQS
660 670 680 690 700
ENILTTSLLM IPLLEIHDNR CNLTPEKQDI NSANKTYTLN KKRPNPNPCK
710 720 730 740 750
AVKLEKSLQS NCEWETVVYG KTEDQLIMTE QARRYLSTYT ATSSTSRALI
L
The sequence of this isoform differs from the canonical sequence as follows:
649-708: Missing.
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4J132 | A0A0R4J132_MOUSE | Myb-related protein A | Mybl1 | 751 | Annotation score: | ||
E9QLX9 | E9QLX9_MOUSE | Myb-related protein A | Mybl1 | 691 | Annotation score: | ||
A0A087WPK9 | A0A087WPK9_MOUSE | Myb-related protein A | Mybl1 | 267 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 358 – 359 | PR → AE in AAA62182 (PubMed:8084617).Curated | 2 | |
Sequence conflicti | 446 | S → L in AAA62182 (PubMed:8084617).Curated | 1 | |
Sequence conflicti | 464 – 465 | SA → DG in AAA62182 (PubMed:8084617).Curated | 2 | |
Sequence conflicti | 473 – 475 | RRI → AAL in AAA62182 (PubMed:8084617).Curated | 3 | |
Sequence conflicti | 751 | L → LYCY in CAA57771 (PubMed:7813437).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 48 | K → R. | 1 | |
Natural varianti | 404 | M → V. | 1 | |
Natural varianti | 447 | R → G. | 1 | |
Natural varianti | 708 | L → P. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003300 | 649 – 708 | Missing in isoform Short. 1 PublicationAdd BLAST | 60 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X82327 mRNA Translation: CAA57771.1 L35261 mRNA Translation: AAA62182.1 |
CCDSi | CCDS35510.1 [P51960-1] CCDS69861.1 [P51960-2] |
PIRi | I49497 |
RefSeqi | NP_032677.2, NM_008651.3 |
Genome annotation databases
GeneIDi | 17864 |
KEGGi | mmu:17864 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X82327 mRNA Translation: CAA57771.1 L35261 mRNA Translation: AAA62182.1 |
CCDSi | CCDS35510.1 [P51960-1] CCDS69861.1 [P51960-2] |
PIRi | I49497 |
RefSeqi | NP_032677.2, NM_008651.3 |
3D structure databases
AlphaFoldDBi | P51960 |
SMRi | P51960 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000086034 |
PTM databases
iPTMneti | P51960 |
PhosphoSitePlusi | P51960 |
Proteomic databases
PaxDbi | P51960 |
PRIDEi | P51960 |
ProteomicsDBi | 286091 [P51960-1] 286092 [P51960-2] |
Protocols and materials databases
DNASUi | 17864 |
Genome annotation databases
GeneIDi | 17864 |
KEGGi | mmu:17864 |
Organism-specific databases
CTDi | 4603 |
MGIi | MGI:99925, Mybl1 |
Phylogenomic databases
eggNOGi | KOG0048, Eukaryota |
InParanoidi | P51960 |
OrthoDBi | 219341at2759 |
PhylomeDBi | P51960 |
TreeFami | TF326257 |
Miscellaneous databases
BioGRID-ORCSi | 17864, 4 hits in 75 CRISPR screens |
ChiTaRSi | Mybl1, mouse |
PROi | PR:P51960 |
RNActi | P51960, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd00167, SANT, 3 hits |
InterProi | View protein in InterPro IPR015395, C-myb_C IPR009057, Homeobox-like_sf IPR017930, Myb_dom IPR028317, MYBL1 IPR001005, SANT/Myb IPR012642, Tscrpt_reg_Wos2-domain |
PANTHERi | PTHR45614:SF9, PTHR45614:SF9, 1 hit |
Pfami | View protein in Pfam PF09316, Cmyb_C, 1 hit PF07988, LMSTEN, 1 hit PF00249, Myb_DNA-binding, 1 hit |
SMARTi | View protein in SMART SM00717, SANT, 3 hits |
SUPFAMi | SSF46689, SSF46689, 2 hits |
PROSITEi | View protein in PROSITE PS51294, HTH_MYB, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | MYBA_MOUSE | |
Accessioni | P51960Primary (citable) accession number: P51960 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 166 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot