UniProtKB - P51952 (CDK7_RAT)
Cyclin-dependent kinase 7
Cdk7
Functioni
Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition (By similarity).
By similarityCatalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 33 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 129 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 18 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP-dependent activity, acting on DNA Source: UniProtKB
- cyclin-dependent protein serine/threonine kinase activity Source: RGD
- kinase activity Source: RGD
- protein-containing complex binding Source: RGD
- protein C-terminus binding Source: RGD
- protein kinase activity Source: RGD
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine kinase activity Source: RHEA
- RNA polymerase II CTD heptapeptide repeat kinase activity Source: UniProtKB
GO - Biological processi
- cell division Source: UniProtKB-KW
- DNA repair Source: UniProtKB-KW
- meiotic cell cycle Source: UniProtKB-KW
- phosphorylation of RNA polymerase II C-terminal domain Source: GO_Central
- positive regulation of transcription by RNA polymerase II Source: GO_Central
- protein phosphorylation Source: RGD
- protein stabilization Source: RGD
- regulation of G1/S transition of mitotic cell cycle Source: RGD
- transcription by RNA polymerase II Source: RGD
- transcription initiation from RNA polymerase II promoter Source: RGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cell cycle, Cell division, DNA damage, DNA repair, Meiosis, Transcription, Transcription regulation |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-112382, Formation of RNA Pol II elongation complex R-RNO-113418, Formation of the Early Elongation Complex R-RNO-5696395, Formation of Incision Complex in GG-NER R-RNO-674695, RNA Polymerase II Pre-transcription Events R-RNO-6781823, Formation of TC-NER Pre-Incision Complex R-RNO-6782135, Dual incision in TC-NER R-RNO-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-RNO-6796648, TP53 Regulates Transcription of DNA Repair Genes R-RNO-6807505, RNA polymerase II transcribes snRNA genes R-RNO-69202, Cyclin E associated events during G1/S transition R-RNO-69231, Cyclin D associated events in G1 R-RNO-69273, Cyclin A/B1/B2 associated events during G2/M transition R-RNO-69656, Cyclin A:Cdk2-associated events at S phase entry R-RNO-72086, mRNA Capping R-RNO-73762, RNA Polymerase I Transcription Initiation R-RNO-73772, RNA Polymerase I Promoter Escape R-RNO-73776, RNA Polymerase II Promoter Escape R-RNO-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-RNO-73863, RNA Polymerase I Transcription Termination R-RNO-75953, RNA Polymerase II Transcription Initiation R-RNO-75955, RNA Polymerase II Transcription Elongation R-RNO-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-RNO-77075, RNA Pol II CTD phosphorylation and interaction with CE R-RNO-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Cdk7 Synonyms:Cak, Cak1, Mo15 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621124, Cdk7 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- perinuclear region By similarity
Note: Colocalizes with PRKCI in the cytoplasm and nucleus. Translocates from the nucleus to cytoplasm and perinuclear region in response to DNA-bound peptides (By similarity).By similarity
Cytoskeleton
- cytoskeleton Source: RGD
Cytosol
- cytosol Source: RGD
Nucleus
- CAK-ERCC2 complex Source: RGD
- cyclin-dependent protein kinase activating kinase holoenzyme complex Source: RGD
- nucleus Source: RGD
- transcription factor TFIIH core complex Source: RGD
- transcription factor TFIIH holo complex Source: RGD
- transcription factor TFIIK complex Source: RGD
Other locations
- cyclin-dependent protein kinase holoenzyme complex Source: RGD
- cytoplasm Source: GO_Central
- membrane Source: RGD
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085793 | ‹1 – ›329 | Cyclin-dependent kinase 7Add BLAST | ›329 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 156 | Phosphoserine; by CDK1 and CDK2By similarity | 1 | |
Modified residuei | 162 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 313 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P51952 |
PTM databases
iPTMneti | P51952 |
PhosphoSitePlusi | P51952 |
Interactioni
Subunit structurei
Associates primarily with cyclin-H (CCNH) and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor; this complex is sensitive to UV light. The CAK complex binds to p53/TP53 in response to DNA damage.
Interacts with CDK2, SF1/NR5A1, PUF60 and PRKCI (By similarity).
Interacts with HINT1 (By similarity).
By similarityGO - Molecular functioni
- protein C-terminus binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000025026 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 287 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 284 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 309 – 329 | DisorderedSequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0659, Eukaryota |
InParanoidi | P51952 |
PhylomeDBi | P51952 |
Family and domain databases
CDDi | cd07841, STKc_CDK7, 1 hit |
InterProi | View protein in InterPro IPR037770, CDK7 IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
PANTHERi | PTHR24056:SF375, PTHR24056:SF375, 1 hit |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
(1+)i Sequence
Sequence statusi: Fragment.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
ANRNEKLDFL GEGQFATVYK ARDKNTNQIV AIKKIKLGHR SEAKDGINRT
60 70 80 90 100
ALREIKLLQE LSHPNIIGLL DAFGHKSNIS LVFDFMETDL EVIIKDNSLV
110 120 130 140 150
LTPSHIKAYM LMTLQGLEYL HQHWILHRDL KPNNLLLDEN GVLKLADFGL
160 170 180 190 200
AKSFGSPNWA YTHQVVTRWY RAPELLFGAR MYGVGVDMWA VGCILAELLL
210 220 230 240 250
RVPFLPGDSD LDQLTRIFET LGTPTEEQWP DMCSLPDYVT FKSFPGIPLQ
260 270 280 290 300
HIFIAAGDDL LELIQGLFLF NPCTRITASQ ALRTKYFSNR PGPTPGCQLP
310 320
RPNCPVEALK EQSNPAMATK RKRAEALEQ
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1LQC8 | F1LQC8_RAT | Cyclin-dependent kinase 7 | Cdk7 | 347 | Annotation score: | ||
A0A8I6ACJ6 | A0A8I6ACJ6_RAT | Cyclin-dependent kinase 7 | Cdk7 | 309 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 | ||
Non-terminal residuei | 329 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83579 mRNA Translation: CAA58562.1 Sequence problems. |
PIRi | S51085 |
Genome annotation databases
UCSCi | RGD:621124, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83579 mRNA Translation: CAA58562.1 Sequence problems. |
PIRi | S51085 |
3D structure databases
AlphaFoldDBi | P51952 |
SMRi | P51952 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000025026 |
PTM databases
iPTMneti | P51952 |
PhosphoSitePlusi | P51952 |
Proteomic databases
jPOSTi | P51952 |
Genome annotation databases
UCSCi | RGD:621124, rat |
Organism-specific databases
RGDi | 621124, Cdk7 |
Phylogenomic databases
eggNOGi | KOG0659, Eukaryota |
InParanoidi | P51952 |
PhylomeDBi | P51952 |
Enzyme and pathway databases
Reactomei | R-RNO-112382, Formation of RNA Pol II elongation complex R-RNO-113418, Formation of the Early Elongation Complex R-RNO-5696395, Formation of Incision Complex in GG-NER R-RNO-674695, RNA Polymerase II Pre-transcription Events R-RNO-6781823, Formation of TC-NER Pre-Incision Complex R-RNO-6782135, Dual incision in TC-NER R-RNO-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-RNO-6796648, TP53 Regulates Transcription of DNA Repair Genes R-RNO-6807505, RNA polymerase II transcribes snRNA genes R-RNO-69202, Cyclin E associated events during G1/S transition R-RNO-69231, Cyclin D associated events in G1 R-RNO-69273, Cyclin A/B1/B2 associated events during G2/M transition R-RNO-69656, Cyclin A:Cdk2-associated events at S phase entry R-RNO-72086, mRNA Capping R-RNO-73762, RNA Polymerase I Transcription Initiation R-RNO-73772, RNA Polymerase I Promoter Escape R-RNO-73776, RNA Polymerase II Promoter Escape R-RNO-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-RNO-73863, RNA Polymerase I Transcription Termination R-RNO-75953, RNA Polymerase II Transcription Initiation R-RNO-75955, RNA Polymerase II Transcription Elongation R-RNO-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-RNO-77075, RNA Pol II CTD phosphorylation and interaction with CE R-RNO-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs |
Family and domain databases
CDDi | cd07841, STKc_CDK7, 1 hit |
InterProi | View protein in InterPro IPR037770, CDK7 IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
PANTHERi | PTHR24056:SF375, PTHR24056:SF375, 1 hit |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CDK7_RAT | |
Accessioni | P51952Primary (citable) accession number: P51952 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | May 10, 2005 | |
Last modified: | May 25, 2022 | |
This is version 165 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families