UniProtKB - P51943 (CCNA2_MOUSE)
Cyclin-A2
Ccna2
Functioni
Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 and CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.
By similarityGO - Molecular functioni
- cyclin-dependent protein serine/threonine kinase regulator activity Source: UniProtKB
- protein domain specific binding Source: MGI
- protein kinase binding Source: MGI
GO - Biological processi
- animal organ regeneration Source: Ensembl
- cell cycle G1/S phase transition Source: UniProtKB
- cell division Source: UniProtKB-KW
- cellular response to cocaine Source: Ensembl
- cellular response to estradiol stimulus Source: Ensembl
- cellular response to hypoxia Source: Ensembl
- cellular response to insulin-like growth factor stimulus Source: Ensembl
- cellular response to leptin stimulus Source: Ensembl
- cellular response to luteinizing hormone stimulus Source: Ensembl
- cellular response to nitric oxide Source: Ensembl
- cellular response to platelet-derived growth factor stimulus Source: Ensembl
- cochlea development Source: Ensembl
- G2/M transition of mitotic cell cycle Source: UniProtKB
- histone phosphorylation Source: MGI
- mitotic cell cycle phase transition Source: GO_Central
- positive regulation of fibroblast proliferation Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- Ras protein signal transduction Source: Ensembl
- regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
- regulation of DNA replication Source: UniProtKB
- response to glucagon Source: Ensembl
Keywordsi
Molecular function | Cyclin |
Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
Reactomei | R-MMU-1538133, G0 and Early G1 R-MMU-171319, Telomere Extension By Telomerase R-MMU-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-MMU-176408, Regulation of APC/C activators between G1/S and early anaphase R-MMU-187577, SCF(Skp2)-mediated degradation of p27/p21 R-MMU-2559582, Senescence-Associated Secretory Phenotype (SASP) R-MMU-2559586, DNA Damage/Telomere Stress Induced Senescence R-MMU-5689880, Ub-specific processing proteases R-MMU-5693607, Processing of DNA double-strand break ends R-MMU-6804116, TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest R-MMU-6804756, Regulation of TP53 Activity through Phosphorylation R-MMU-6804757, Regulation of TP53 Degradation R-MMU-68911, G2 Phase R-MMU-68949, Orc1 removal from chromatin R-MMU-69017, CDK-mediated phosphorylation and removal of Cdc6 R-MMU-69273, Cyclin A/B1/B2 associated events during G2/M transition R-MMU-69563, p53-Dependent G1 DNA Damage Response R-MMU-69656, Cyclin A:Cdk2-associated events at S phase entry |
Names & Taxonomyi
Protein namesi | Recommended name: Cyclin-A21 PublicationShort name: Cyclin-A1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:108069, Ccna2 |
VEuPathDBi | HostDB:ENSMUSG00000027715 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- female pronucleus Source: MGI
- male pronucleus Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cyclin A2-CDK2 complex Source: MGI
- cyclin-dependent protein kinase holoenzyme complex Source: MGI
- cytoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080340 | 1 – 422 | Cyclin-A2Add BLAST | 422 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 5 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P51943 |
MaxQBi | P51943 |
PaxDbi | P51943 |
PeptideAtlasi | P51943 |
PRIDEi | P51943 |
ProteomicsDBi | 280010 |
PTM databases
iPTMneti | P51943 |
PhosphoSitePlusi | P51943 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000027715, Expressed in undifferentiated genital tubercle and 281 other tissues |
Genevisiblei | P51943, MM |
Interactioni
Subunit structurei
Interacts with the CDK1 and CDK2 protein kinases to form serine/threonine kinase holoenzyme complexes (PubMed:10068472).
Interacts with CDK1 (hyperphosphorylated form in G1 and underphosphorylated forms in S and G2).
Interacts with CDK2; the interaction increases from G1 to G2.
Interacts (associated with CDK2 but not with CDK1) with SCAPER; regulates the activity of CCNA2/CDK2 by transiently maintaining CCNA2 in the cytoplasm.
Forms a ternary complex with CDK2 and CDKN1B; CDKN1B inhibits the kinase activity of CDK2 through conformational rearrangements (By similarity).
Interacts with INCA1 (By similarity).
By similarity1 Publication(Microbial infection) Interacts with mouse cytomegalovirus/MCMV kinase M97; this interaction sequesters CCNA2 to the cytoplasm.
1 PublicationBinary interactionsi
P51943
With | #Exp. | IntAct |
---|---|---|
Cdk1 [P11440] | 2 | EBI-846980,EBI-846949 |
Cdk2 [P97377] | 3 | EBI-846980,EBI-847048 |
CDK2 [P24941] from Homo sapiens. | 2 | EBI-846980,EBI-375096 |
GO - Molecular functioni
- protein domain specific binding Source: MGI
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 198545, 11 interactors |
ComplexPortali | CPX-2062, Cyclin A2-CDK1 complex CPX-2066, Cyclin A2-CDK2 complex |
CORUMi | P51943 |
DIPi | DIP-45864N |
IntActi | P51943, 4 interactors |
STRINGi | 10090.ENSMUSP00000029270 |
Miscellaneous databases
RNActi | P51943, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P51943 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 62 | DisorderedSequence analysisAdd BLAST | 62 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0654, Eukaryota |
GeneTreei | ENSGT00940000155372 |
HOGENOMi | CLU_020695_3_2_1 |
InParanoidi | P51943 |
OMAi | GSKYHEV |
OrthoDBi | 993640at2759 |
PhylomeDBi | P51943 |
TreeFami | TF101002 |
Family and domain databases
CDDi | cd00043, CYCLIN, 2 hits |
IDEALi | IID50253 |
InterProi | View protein in InterPro IPR039361, Cyclin IPR032447, Cyclin-A_N IPR013763, Cyclin-like IPR036915, Cyclin-like_sf IPR004367, Cyclin_C-dom IPR006671, Cyclin_N |
PANTHERi | PTHR10177, PTHR10177, 1 hit |
Pfami | View protein in Pfam PF02984, Cyclin_C, 1 hit PF00134, Cyclin_N, 1 hit PF16500, Cyclin_N2, 1 hit |
PIRSFi | PIRSF001771, Cyclin_A_B_D_E, 1 hit |
SMARTi | View protein in SMART SM00385, CYCLIN, 2 hits SM01332, Cyclin_C, 1 hit |
SUPFAMi | SSF47954, SSF47954, 2 hits |
PROSITEi | View protein in PROSITE PS00292, CYCLINS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MPGTSRHSGR DAGSALLSLH QEDQENVNPE KLAPAQQPRA QAVLKAGNVR
60 70 80 90 100
GPAPQQKLKT RRVAPLKDLP INDEHVTAGP SWKAVSKQPA FTIHVDEAEE
110 120 130 140 150
TQKRPAELKE TECEDALAFN AAVSLPGARK PLTPLDYPMD GSFESPHAMD
160 170 180 190 200
MSIVLEDKPV NVNEVPDYQE DIHTYLREME VKCKPKVGYM KRQPDITNSM
210 220 230 240 250
RAILVDWLVE VGEEYKLQNE TLHLAVNYID RFLSSMSVLR GKLQLVGTAA
260 270 280 290 300
MLLASKFEEI YPPEVAEFVY ITDDTYSKKQ VLRMEHLVLK VLAFDLAAPT
310 320 330 340 350
VNQFLTQYFL HLQPANCKVE SLAMFLGELS LIDADPYLKY LPSLIAGAAF
360 370 380 390 400
HLALYTVTGQ SWPESLAQQT GYTLESLKPC LVDLHQTYLK APQHAQQSIR
410 420
EKYKHSKYHS VSLLNPPETL SV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JEY1 | A0A0G2JEY1_MOUSE | Cyclin-A2 | Ccna2 | 285 | Annotation score: | ||
D6RIK7 | D6RIK7_MOUSE | Cyclin-A2 | Ccna2 | 153 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 127 | G → A in CAA81331 (PubMed:8575639).Curated | 1 | |
Sequence conflicti | 127 | G → A in CAA53212 (PubMed:8565853).Curated | 1 | |
Sequence conflicti | 391 | A → P in CAA53212 (PubMed:8565853).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z26580 mRNA Translation: CAA81331.1 X75483 mRNA Translation: CAA53212.1 AK044924 mRNA Translation: BAC32144.1 CH466530 Genomic DNA Translation: EDL35081.1 BC052730 mRNA Translation: AAH52730.1 |
CCDSi | CCDS17313.1 |
PIRi | S37280 S38501 |
RefSeqi | NP_033958.2, NM_009828.2 XP_017174933.1, XM_017319444.1 |
Genome annotation databases
Ensembli | ENSMUST00000029270; ENSMUSP00000029270; ENSMUSG00000027715 |
GeneIDi | 12428 |
KEGGi | mmu:12428 |
UCSCi | uc012cov.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z26580 mRNA Translation: CAA81331.1 X75483 mRNA Translation: CAA53212.1 AK044924 mRNA Translation: BAC32144.1 CH466530 Genomic DNA Translation: EDL35081.1 BC052730 mRNA Translation: AAH52730.1 |
CCDSi | CCDS17313.1 |
PIRi | S37280 S38501 |
RefSeqi | NP_033958.2, NM_009828.2 XP_017174933.1, XM_017319444.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3QHR | X-ray | 2.17 | B/D | 163-422 | [»] | |
3QHW | X-ray | 1.91 | B/D | 163-422 | [»] | |
4I3Z | X-ray | 2.05 | B/D | 165-421 | [»] | |
4II5 | X-ray | 2.15 | B/D | 165-422 | [»] | |
SMRi | P51943 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198545, 11 interactors |
ComplexPortali | CPX-2062, Cyclin A2-CDK1 complex CPX-2066, Cyclin A2-CDK2 complex |
CORUMi | P51943 |
DIPi | DIP-45864N |
IntActi | P51943, 4 interactors |
STRINGi | 10090.ENSMUSP00000029270 |
PTM databases
iPTMneti | P51943 |
PhosphoSitePlusi | P51943 |
Proteomic databases
EPDi | P51943 |
MaxQBi | P51943 |
PaxDbi | P51943 |
PeptideAtlasi | P51943 |
PRIDEi | P51943 |
ProteomicsDBi | 280010 |
Protocols and materials databases
Antibodypediai | 3665, 778 antibodies from 44 providers |
DNASUi | 12428 |
Genome annotation databases
Ensembli | ENSMUST00000029270; ENSMUSP00000029270; ENSMUSG00000027715 |
GeneIDi | 12428 |
KEGGi | mmu:12428 |
UCSCi | uc012cov.1, mouse |
Organism-specific databases
CTDi | 890 |
MGIi | MGI:108069, Ccna2 |
VEuPathDBi | HostDB:ENSMUSG00000027715 |
Phylogenomic databases
eggNOGi | KOG0654, Eukaryota |
GeneTreei | ENSGT00940000155372 |
HOGENOMi | CLU_020695_3_2_1 |
InParanoidi | P51943 |
OMAi | GSKYHEV |
OrthoDBi | 993640at2759 |
PhylomeDBi | P51943 |
TreeFami | TF101002 |
Enzyme and pathway databases
Reactomei | R-MMU-1538133, G0 and Early G1 R-MMU-171319, Telomere Extension By Telomerase R-MMU-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-MMU-176408, Regulation of APC/C activators between G1/S and early anaphase R-MMU-187577, SCF(Skp2)-mediated degradation of p27/p21 R-MMU-2559582, Senescence-Associated Secretory Phenotype (SASP) R-MMU-2559586, DNA Damage/Telomere Stress Induced Senescence R-MMU-5689880, Ub-specific processing proteases R-MMU-5693607, Processing of DNA double-strand break ends R-MMU-6804116, TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest R-MMU-6804756, Regulation of TP53 Activity through Phosphorylation R-MMU-6804757, Regulation of TP53 Degradation R-MMU-68911, G2 Phase R-MMU-68949, Orc1 removal from chromatin R-MMU-69017, CDK-mediated phosphorylation and removal of Cdc6 R-MMU-69273, Cyclin A/B1/B2 associated events during G2/M transition R-MMU-69563, p53-Dependent G1 DNA Damage Response R-MMU-69656, Cyclin A:Cdk2-associated events at S phase entry |
Miscellaneous databases
BioGRID-ORCSi | 12428, 24 hits in 64 CRISPR screens |
ChiTaRSi | Ccna2, mouse |
PROi | PR:P51943 |
RNActi | P51943, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027715, Expressed in undifferentiated genital tubercle and 281 other tissues |
Genevisiblei | P51943, MM |
Family and domain databases
CDDi | cd00043, CYCLIN, 2 hits |
IDEALi | IID50253 |
InterProi | View protein in InterPro IPR039361, Cyclin IPR032447, Cyclin-A_N IPR013763, Cyclin-like IPR036915, Cyclin-like_sf IPR004367, Cyclin_C-dom IPR006671, Cyclin_N |
PANTHERi | PTHR10177, PTHR10177, 1 hit |
Pfami | View protein in Pfam PF02984, Cyclin_C, 1 hit PF00134, Cyclin_N, 1 hit PF16500, Cyclin_N2, 1 hit |
PIRSFi | PIRSF001771, Cyclin_A_B_D_E, 1 hit |
SMARTi | View protein in SMART SM00385, CYCLIN, 2 hits SM01332, Cyclin_C, 1 hit |
SUPFAMi | SSF47954, SSF47954, 2 hits |
PROSITEi | View protein in PROSITE PS00292, CYCLINS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CCNA2_MOUSE | |
Accessioni | P51943Primary (citable) accession number: P51943 Secondary accession number(s): Q61459, Q8BRG1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 168 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families