UniProtKB - P51859 (HDGF_MOUSE)
Protein
Hepatoma-derived growth factor
Gene
Hdgf
Organism
Mus musculus (Mouse)
Status
Functioni
Acts as a transcriptional repressor (By similarity). Has mitogenic activity for fibroblasts (By similarity). Heparin-binding protein (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 19 | HeparinBy similarity | 1 | |
Binding sitei | 21 | HeparinBy similarity | 1 | |
Binding sitei | 72 | HeparinBy similarity | 1 | |
Binding sitei | 75 | HeparinBy similarity | 1 | |
Binding sitei | 79 | HeparinBy similarity | 1 | |
Binding sitei | 80 | HeparinBy similarity | 1 |
GO - Molecular functioni
- actin binding Source: MGI
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- growth factor activity Source: MGI
- heparin binding Source: UniProtKB
- nucleotide binding Source: UniProtKB-KW
- RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI
- transcription corepressor activity Source: MGI
- transcription corepressor binding Source: MGI
- tubulin binding Source: MGI
GO - Biological processi
- cellular process Source: MGI
- cellular response to interleukin-7 Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of cell division Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
- protein localization to nucleus Source: MGI
Keywordsi
Molecular function | DNA-binding, Growth factor, Heparin-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Hepatoma-derived growth factorShort name: HDGF |
Gene namesi | Name:Hdgf Synonyms:Tdrm1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1194494 Hdgf |
Subcellular locationi
Nucleus
- Nucleus By similarity
Extracellular region or secreted
- extracellular exosome 1 Publication
Other locations
- Cytoplasm By similarity
Note: Secreted by exosomes and is located inside the exosome (By similarity). May also be secreted as free protein via an as yet unknown pathway (By similarity).By similarity
Extracellular region or secreted
- extracellular space Source: UniProtKB
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- transcriptional repressor complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, Nucleus, SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 12 | C → A: Abolishes disulfide bond formation. 1 Publication | 1 | |
Mutagenesisi | 98 | S → A: Does not affect secretion. 1 Publication | 1 | |
Mutagenesisi | 102 – 103 | SS → AA: Does not affect secretion. 1 Publication | 2 | |
Mutagenesisi | 108 | C → A: Abolishes disulfide bond formation. 1 Publication | 1 | |
Mutagenesisi | 128 | S → A: Does not affect secretion. 1 Publication | 1 | |
Mutagenesisi | 132 | S → A: Does not affect secretion. 1 Publication | 1 | |
Mutagenesisi | 133 | S → A: Does not affect secretion. 1 Publication | 1 | |
Mutagenesisi | 165 | S → A: Abolishes secretion. 1 Publication | 1 | |
Mutagenesisi | 202 | S → A: Does not affect secretion. 1 Publication | 1 | |
Mutagenesisi | 206 | S → A: Does not affect secretion. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000191701 | 1 – 237 | Hepatoma-derived growth factorAdd BLAST | 237 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 12 ↔ 108 | 1 Publication | ||
Modified residuei | 44 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 80 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity | ||
Cross-linki | 80 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 128 | PhosphoserineCombined sources | 1 | |
Modified residuei | 132 | PhosphoserineCombined sources | 1 | |
Modified residuei | 133 | PhosphoserineCombined sources | 1 | |
Modified residuei | 165 | PhosphoserineCombined sources | 1 | |
Modified residuei | 199 | PhosphoserineBy similarity | 1 | |
Modified residuei | 200 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 202 | PhosphoserineCombined sources | 1 | |
Modified residuei | 206 | PhosphoserineCombined sources | 1 | |
Modified residuei | 236 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Sumoylated with SUMO1. Sumoylation prevents binding to chromatin.By similarity
Phosphorylation at Ser-165 is likely to be required for secretion.1 Publication
Keywords - PTMi
Acetylation, Disulfide bond, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P51859 |
jPOSTi | P51859 |
MaxQBi | P51859 |
PaxDbi | P51859 |
PeptideAtlasi | P51859 |
PRIDEi | P51859 |
TopDownProteomicsi | P51859 |
PTM databases
iPTMneti | P51859 |
PhosphoSitePlusi | P51859 |
SwissPalmi | P51859 |
Miscellaneous databases
PMAP-CutDBi | P51859 |
Expressioni
Tissue specificityi
Expressed predominantly in testis and skeletal muscle, to intermediate extents in heart, brain, lung, liver, and kidney, and to a minimal extent in spleen.
Gene expression databases
Bgeei | ENSMUSG00000004897 Expressed in 316 organ(s), highest expression level in ear vesicle |
ExpressionAtlasi | P51859 baseline and differential |
Genevisiblei | P51859 MM |
Interactioni
Subunit structurei
Monomer, and domain-swapped homodimer (By similarity).
Interacts with nuclear proteins NCL and YBX1/YB1 (By similarity).
By similarityBinary interactionsi
GO - Molecular functioni
- actin binding Source: MGI
- growth factor activity Source: MGI
- transcription corepressor binding Source: MGI
- tubulin binding Source: MGI
Protein-protein interaction databases
BioGridi | 200265, 2 interactors |
IntActi | P51859, 8 interactors |
MINTi | P51859 |
STRINGi | 10090.ENSMUSP00000005017 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 69 | PWWPPROSITE-ProRule annotationAdd BLAST | 58 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 75 – 80 | Nuclear localization signalBy similarity | 6 | |
Motifi | 155 – 170 | Bipartite nuclear localization signalBy similarityAdd BLAST | 16 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 111 – 227 | Glu-richAdd BLAST | 117 |
Domaini
The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured.By similarity
The N-terminal region does not contain a typical signal sequence but is required for secretion (PubMed:21087088). It also determines exosomal location (By similarity).By similarity1 Publication
Sequence similaritiesi
Belongs to the HDGF family.Curated
Phylogenomic databases
eggNOGi | ENOG410IMJ5 Eukaryota ENOG4111IFT LUCA |
GeneTreei | ENSGT00940000157485 |
HOGENOMi | HOG000112874 |
InParanoidi | P51859 |
KOi | K16641 |
OrthoDBi | 530959at2759 |
PhylomeDBi | P51859 |
TreeFami | TF105385 |
Family and domain databases
CDDi | cd05834 HDGF_related, 1 hit |
InterProi | View protein in InterPro IPR035496 HDGF-rel_PWWP IPR000313 PWWP_dom |
Pfami | View protein in Pfam PF00855 PWWP, 1 hit |
SMARTi | View protein in SMART SM00293 PWWP, 1 hit |
PROSITEi | View protein in PROSITE PS50812 PWWP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P51859-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRSNRQKEY KCGDLVFAKM KGYPHWPARI DEMPEAAVKS TANKYQVFFF
60 70 80 90 100
GTHETAFLGP KDLFPYEESK EKFGKPNKRK GFSEGLWEIE NNPTVKASGY
110 120 130 140 150
QSSQKKSCAA EPEVEPEAHE GDGDKKGSAE GSSDEEGKLV IDEPAKEKNE
160 170 180 190 200
KGTLKRRAGD VLEDSPKRPK ESGDHEEEDK EIAALEGERP LPVEVEKNST
210 220 230
PSEPDSGQGP PAEEEEGEEE AAKEEAEAQG VRDHESL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE0CXA0 | E0CXA0_MOUSE | Hepatoma-derived growth factor | Hdgf | 202 | Annotation score: | ||
E0CYW7 | E0CYW7_MOUSE | Hepatoma-derived growth factor | Hdgf | 84 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 118 | A → D in BAB30979 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 190 | P → H in BAA09838 (PubMed:9299445).Curated | 1 | |
Sequence conflicti | 229 | Q → E in BAC36126 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 229 | Q → P in BAA09838 (PubMed:9299445).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D63707 mRNA Translation: BAA09838.1 AF251787 mRNA Translation: AAF65469.1 AK017863 mRNA Translation: BAB30979.1 AK029475 mRNA Translation: BAC26466.1 AK076021 mRNA Translation: BAC36126.1 BC005713 mRNA Translation: AAH05713.1 BC021654 mRNA Translation: AAH21654.1 |
CCDSi | CCDS17457.1 |
PIRi | JC5660 |
RefSeqi | NP_032257.3, NM_008231.4 |
Genome annotation databases
Ensembli | ENSMUST00000005017; ENSMUSP00000005017; ENSMUSG00000004897 |
GeneIDi | 15191 |
KEGGi | mmu:15191 |
UCSCi | uc008ptc.2 mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D63707 mRNA Translation: BAA09838.1 AF251787 mRNA Translation: AAF65469.1 AK017863 mRNA Translation: BAB30979.1 AK029475 mRNA Translation: BAC26466.1 AK076021 mRNA Translation: BAC36126.1 BC005713 mRNA Translation: AAH05713.1 BC021654 mRNA Translation: AAH21654.1 |
CCDSi | CCDS17457.1 |
PIRi | JC5660 |
RefSeqi | NP_032257.3, NM_008231.4 |
3D structure databases
SMRi | P51859 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 200265, 2 interactors |
IntActi | P51859, 8 interactors |
MINTi | P51859 |
STRINGi | 10090.ENSMUSP00000005017 |
PTM databases
iPTMneti | P51859 |
PhosphoSitePlusi | P51859 |
SwissPalmi | P51859 |
Proteomic databases
EPDi | P51859 |
jPOSTi | P51859 |
MaxQBi | P51859 |
PaxDbi | P51859 |
PeptideAtlasi | P51859 |
PRIDEi | P51859 |
TopDownProteomicsi | P51859 |
Genome annotation databases
Ensembli | ENSMUST00000005017; ENSMUSP00000005017; ENSMUSG00000004897 |
GeneIDi | 15191 |
KEGGi | mmu:15191 |
UCSCi | uc008ptc.2 mouse |
Organism-specific databases
CTDi | 3068 |
MGIi | MGI:1194494 Hdgf |
Phylogenomic databases
eggNOGi | ENOG410IMJ5 Eukaryota ENOG4111IFT LUCA |
GeneTreei | ENSGT00940000157485 |
HOGENOMi | HOG000112874 |
InParanoidi | P51859 |
KOi | K16641 |
OrthoDBi | 530959at2759 |
PhylomeDBi | P51859 |
TreeFami | TF105385 |
Miscellaneous databases
ChiTaRSi | Hdgf mouse |
PMAP-CutDBi | P51859 |
PROi | PR:P51859 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000004897 Expressed in 316 organ(s), highest expression level in ear vesicle |
ExpressionAtlasi | P51859 baseline and differential |
Genevisiblei | P51859 MM |
Family and domain databases
CDDi | cd05834 HDGF_related, 1 hit |
InterProi | View protein in InterPro IPR035496 HDGF-rel_PWWP IPR000313 PWWP_dom |
Pfami | View protein in Pfam PF00855 PWWP, 1 hit |
SMARTi | View protein in SMART SM00293 PWWP, 1 hit |
PROSITEi | View protein in PROSITE PS50812 PWWP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HDGF_MOUSE | |
Accessioni | P51859Primary (citable) accession number: P51859 Secondary accession number(s): Q8BPG7, Q9CYA4, Q9JK87 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | August 13, 2002 | |
Last modified: | September 18, 2019 | |
This is version 162 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot