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Protein

Glutathione synthetase

Gene

Gss

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase regulatory subunit (Gclm), Glutamate--cysteine ligase catalytic subunit (Gclc)
  2. Glutathione synthetase (Gss), Glutathione synthetase (Gss), Glutathione synthetase (Gss)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei125SubstrateBy similarity1
Metal bindingi144MagnesiumBy similarity1
Binding sitei144ATPBy similarity1
Metal bindingi146MagnesiumBy similarity1
Binding sitei220SubstrateBy similarity1
Binding sitei305ATPBy similarity1
Metal bindingi368MagnesiumBy similarity1
Binding sitei375ATPBy similarity1
Binding sitei425ATPBy similarity1
Binding sitei450SubstrateBy similarity1
Binding sitei452ATPBy similarity1
Binding sitei458ATP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi364 – 373ATPBy similarity10
Nucleotide bindingi398 – 401ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processGlutathione biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-174403 Glutathione synthesis and recycling
SABIO-RKiP51855
UniPathwayi
UPA00142;UER00210

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Alternative name(s):
Glutathione synthase
Gene namesi
Name:Gss
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95852 Gss

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002112622 – 474Glutathione synthetaseAdd BLAST473

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei415PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP51855
PaxDbiP51855
PeptideAtlasiP51855
PRIDEiP51855

PTM databases

iPTMnetiP51855
PhosphoSitePlusiP51855

Expressioni

Gene expression databases

BgeeiENSMUSG00000027610 Expressed in 241 organ(s), highest expression level in lens of camera-type eye
CleanExiMM_GSS
ExpressionAtlasiP51855 baseline and differential
GenevisibleiP51855 MM

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP51855, 2 interactors
MINTiP51855
STRINGi10090.ENSMUSP00000078630

Structurei

3D structure databases

ProteinModelPortaliP51855
SMRiP51855
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 151Substrate bindingBy similarity4
Regioni214 – 216Substrate bindingBy similarity3
Regioni267 – 270Substrate bindingBy similarity4
Regioni461 – 462Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.Curated

Phylogenomic databases

eggNOGiKOG0021 Eukaryota
ENOG410XPHH LUCA
GeneTreeiENSGT00390000013764
HOGENOMiHOG000172641
HOVERGENiHBG002458
InParanoidiP51855
KOiK21456
OMAiFRSDYMV
OrthoDBiEOG091G05V8
PhylomeDBiP51855
TreeFamiTF105187

Family and domain databases

CDDicd00228 eu-GS, 1 hit
Gene3Di1.10.1080.10, 2 hits
3.30.1490.50, 1 hit
3.30.1490.80, 3 hits
3.40.50.1760, 1 hit
InterProiView protein in InterPro
IPR005615 Glutathione_synthase
IPR014042 Glutathione_synthase_a-hlx
IPR014709 Glutathione_synthase_C_euk
IPR014049 Glutathione_synthase_N_euk
IPR037013 GSH-S_sub-bd_sf
IPR004887 GSH_synth_subst-bd
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR11130 PTHR11130, 1 hit
PfamiView protein in Pfam
PF03917 GSH_synth_ATP, 1 hit
PF03199 GSH_synthase, 1 hit
PIRSFiPIRSF001558 GSHase, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01986 glut_syn_euk, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

P51855-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATSWGSILQ DEKQLEELAK QAIDRALAEG VLLRSAQHPS SSDVVTYAPF
60 70 80 90 100
TLFPSPVPSA LLEQAYAVQM DFNILVDAVS QNPAFLEQTL SSTIKKDDYT
110 120 130 140 150
ARLFDIYKQV LKEGIAQTVF LGLNRSDYMF QCGADGSKAL KQIEINTISA
160 170 180 190 200
SFGGLASRTP AVHRHVLNVL NKTKEASKIL SNNPSKGLAL GIAKAWELYG
210 220 230 240 250
SANAVVLLIA QEKERNIFDQ RAVENELLDR KIHVIRGRFE DVSERGSLDQ
260 270 280 290 300
NRRLFMDDQE VAVVYFRDGY MPSQYNSQNW EARLMLERSR AAKCPDIAIQ
310 320 330 340 350
LAGTKKVQQE LSRVGLLEAL LPGQPEAVAR LRATFAGLYS LDMGEEGDQA
360 370 380 390 400
IAEALAAPSH FVLKPQREGG GNNLYGEEMV QALEQLKDSE ERASYILMEK
410 420 430 440 450
IEPEPFRNCL LRPGSPAQVV QCISELGIFG VYVRQGTTLV MNKHVGHLLR
460 470
TKAVEHADGG VAAGVAVLDN PYPV
Length:474
Mass (Da):52,247
Last modified:October 1, 1996 - v1
Checksum:iE1302C5B2C28A43A
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3UEE2Q3UEE2_MOUSE
Glutathione synthetase
Gss
405Annotation score:
A2AQN9A2AQN9_MOUSE
Glutathione synthetase
Gss
179Annotation score:
H3BKH4H3BKH4_MOUSE
Glutathione synthetase
Gss
216Annotation score:
A2AQN7A2AQN7_MOUSE
Glutathione synthetase
Gss
200Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35456 mRNA Translation: AAB09730.1
BC003784 mRNA Translation: AAH03784.1
CCDSiCCDS16951.1
PIRiS71322
RefSeqiNP_032206.1, NM_008180.2
XP_006498844.1, XM_006498781.2
UniGeneiMm.252316

Genome annotation databases

EnsembliENSMUST00000079691; ENSMUSP00000078630; ENSMUSG00000027610
GeneIDi14854
KEGGimmu:14854
UCSCiuc008nkx.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35456 mRNA Translation: AAB09730.1
BC003784 mRNA Translation: AAH03784.1
CCDSiCCDS16951.1
PIRiS71322
RefSeqiNP_032206.1, NM_008180.2
XP_006498844.1, XM_006498781.2
UniGeneiMm.252316

3D structure databases

ProteinModelPortaliP51855
SMRiP51855
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP51855, 2 interactors
MINTiP51855
STRINGi10090.ENSMUSP00000078630

PTM databases

iPTMnetiP51855
PhosphoSitePlusiP51855

Proteomic databases

EPDiP51855
PaxDbiP51855
PeptideAtlasiP51855
PRIDEiP51855

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079691; ENSMUSP00000078630; ENSMUSG00000027610
GeneIDi14854
KEGGimmu:14854
UCSCiuc008nkx.2 mouse

Organism-specific databases

CTDi2937
MGIiMGI:95852 Gss

Phylogenomic databases

eggNOGiKOG0021 Eukaryota
ENOG410XPHH LUCA
GeneTreeiENSGT00390000013764
HOGENOMiHOG000172641
HOVERGENiHBG002458
InParanoidiP51855
KOiK21456
OMAiFRSDYMV
OrthoDBiEOG091G05V8
PhylomeDBiP51855
TreeFamiTF105187

Enzyme and pathway databases

UniPathwayi
UPA00142;UER00210

ReactomeiR-MMU-174403 Glutathione synthesis and recycling
SABIO-RKiP51855

Miscellaneous databases

PROiPR:P51855
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027610 Expressed in 241 organ(s), highest expression level in lens of camera-type eye
CleanExiMM_GSS
ExpressionAtlasiP51855 baseline and differential
GenevisibleiP51855 MM

Family and domain databases

CDDicd00228 eu-GS, 1 hit
Gene3Di1.10.1080.10, 2 hits
3.30.1490.50, 1 hit
3.30.1490.80, 3 hits
3.40.50.1760, 1 hit
InterProiView protein in InterPro
IPR005615 Glutathione_synthase
IPR014042 Glutathione_synthase_a-hlx
IPR014709 Glutathione_synthase_C_euk
IPR014049 Glutathione_synthase_N_euk
IPR037013 GSH-S_sub-bd_sf
IPR004887 GSH_synth_subst-bd
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR11130 PTHR11130, 1 hit
PfamiView protein in Pfam
PF03917 GSH_synth_ATP, 1 hit
PF03199 GSH_synthase, 1 hit
PIRSFiPIRSF001558 GSHase, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01986 glut_syn_euk, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGSHB_MOUSE
AccessioniPrimary (citable) accession number: P51855
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 12, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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