UniProtKB - P51833 (RNC_BACSU)
Ribonuclease 3
rnc
Functioni
Digests double-stranded RNA. Involved in the processing of ribosomal RNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes pre-scRNA (the precursor of the signal recognition particle RNA). Probably also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon (PubMed:22229825, PubMed:26940229, PubMed:11123676, PubMed:17576666, PubMed:18363798, PubMed:22412379).
Probably processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism (Probable). Involved in degradation of type I toxin-antitoxin system duplex RNAs (PubMed:22229825, PubMed:26940229).
Curated6 PublicationsCatalytic activityi
- Endonucleolytic cleavage to 5'-phosphomonoester. EC:3.1.26.3
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 62 | MagnesiumBy similarity | 1 | |
Active sitei | 66 | By similarity | 1 | |
Metal bindingi | 135 | MagnesiumBy similarity | 1 | |
Active sitei | 138 | By similarity | 1 | |
Metal bindingi | 138 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- double-stranded RNA binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- ribonuclease III activity Source: GO_Central
GO - Biological processi
- mRNA processing Source: UniProtKB-UniRule
- regulation of gene expression Source: GO_Central
- RNA processing Source: GO_Central
- rRNA catabolic process Source: InterPro
- rRNA processing Source: UniProtKB-UniRule
- tRNA processing Source: UniProtKB-KW
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease, RNA-binding |
Biological process | mRNA processing, rRNA processing, tRNA processing |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU15930-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Ribonuclease 3 (EC:3.1.26.3)Alternative name(s): Ribonuclease III Short name: RNase III |
Gene namesi | Name:rnc Synonyms:rncS Ordered Locus Names:BSU15930 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000180375 | 1 – 249 | Ribonuclease 3Add BLAST | 249 |
Proteomic databases
PaxDbi | P51833 |
PRIDEi | P51833 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 224308.BSU15930 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 149 | RNase IIIAdd BLAST | 130 | |
Domaini | 175 – 244 | DRBMAdd BLAST | 70 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 218 – 249 | DisorderedSequence analysisAdd BLAST | 32 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 222 – 249 | Basic and acidic residuesSequence analysisAdd BLAST | 28 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0571, Bacteria |
InParanoidi | P51833 |
OMAi | LTHKSCK |
PhylomeDBi | P51833 |
Family and domain databases
CDDi | cd00593, RIBOc, 1 hit |
Gene3Di | 1.10.1520.10, 1 hit |
HAMAPi | MF_00104, RNase_III, 1 hit |
InterProi | View protein in InterPro IPR014720, dsRBD_dom IPR011907, RNase_III IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
PANTHERi | PTHR11207, PTHR11207, 2 hits |
Pfami | View protein in Pfam PF00035, dsrm, 1 hit PF14622, Ribonucleas_3_3, 1 hit |
SMARTi | View protein in SMART SM00358, DSRM, 1 hit SM00535, RIBOc, 1 hit |
SUPFAMi | SSF69065, SSF69065, 1 hit |
TIGRFAMsi | TIGR02191, RNaseIII, 1 hit |
PROSITEi | View protein in PROSITE PS50137, DS_RBD, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSKHSHYKDK KKFYKKVEQF KEFQERISVH FQNEKLLYQA FTHSSYVNEH
60 70 80 90 100
RKKPYEDNER LEFLGDAVLE LTISRFLFAK YPAMSEGDLT KLRAAIVCEP
110 120 130 140 150
SLVSLAHELS FGDLVLLGKG EEMTGGRKRP ALLADVFEAF IGALYLDQGL
160 170 180 190 200
EPVESFLKVY VFPKINDGAF SHVMDFKSQL QEYVQRDGKG SLEYKISNEK
210 220 230 240
GPAHNREFEA IVSLKGEPLG VGNGRSKKEA EQHAAQEALA KLQKHHTKQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 79 | A → P in BAA10976 (PubMed:8654983).Curated | 1 | |
Sequence conflicti | 171 | S → P in BAA10976 (PubMed:8654983).Curated | 1 | |
Sequence conflicti | 243 | Q → E in BAA10976 (PubMed:8654983).Curated | 1 | |
Sequence conflicti | 249 | Q → QLNPPYDSGGFQYVCRLI in BAA10976 (PubMed:8654983).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D64116 Genomic DNA Translation: BAA10976.1 AL009126 Genomic DNA Translation: CAB13466.1 |
PIRi | B69693 |
RefSeqi | NP_389475.1, NC_000964.3 WP_003232030.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB13466; CAB13466; BSU_15930 |
GeneIDi | 936169 |
KEGGi | bsu:BSU15930 |
PATRICi | fig|224308.179.peg.1733 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D64116 Genomic DNA Translation: BAA10976.1 AL009126 Genomic DNA Translation: CAB13466.1 |
PIRi | B69693 |
RefSeqi | NP_389475.1, NC_000964.3 WP_003232030.1, NZ_JNCM01000035.1 |
3D structure databases
SMRi | P51833 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU15930 |
Proteomic databases
PaxDbi | P51833 |
PRIDEi | P51833 |
Genome annotation databases
EnsemblBacteriai | CAB13466; CAB13466; BSU_15930 |
GeneIDi | 936169 |
KEGGi | bsu:BSU15930 |
PATRICi | fig|224308.179.peg.1733 |
Phylogenomic databases
eggNOGi | COG0571, Bacteria |
InParanoidi | P51833 |
OMAi | LTHKSCK |
PhylomeDBi | P51833 |
Enzyme and pathway databases
BioCyci | BSUB:BSU15930-MONOMER |
Family and domain databases
CDDi | cd00593, RIBOc, 1 hit |
Gene3Di | 1.10.1520.10, 1 hit |
HAMAPi | MF_00104, RNase_III, 1 hit |
InterProi | View protein in InterPro IPR014720, dsRBD_dom IPR011907, RNase_III IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
PANTHERi | PTHR11207, PTHR11207, 2 hits |
Pfami | View protein in Pfam PF00035, dsrm, 1 hit PF14622, Ribonucleas_3_3, 1 hit |
SMARTi | View protein in SMART SM00358, DSRM, 1 hit SM00535, RIBOc, 1 hit |
SUPFAMi | SSF69065, SSF69065, 1 hit |
TIGRFAMsi | TIGR02191, RNaseIII, 1 hit |
PROSITEi | View protein in PROSITE PS50137, DS_RBD, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RNC_BACSU | |
Accessioni | P51833Primary (citable) accession number: P51833 Secondary accession number(s): O31734 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | May 30, 2000 | |
Last modified: | February 23, 2022 | |
This is version 136 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families