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Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene
N/A
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Can play two different roles depending on the source of dihydrofolate: de novo synthesis of tetrahydrofolate or recycling of the dihydrofolate released as one of the end products of the TS catalyzed reaction. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional dihydrofolate reductase-thymidylate synthase (100786329), Bifunctional dihydrofolate reductase-thymidylate synthase (547833), Bifunctional dihydrofolate reductase-thymidylate synthase (100788658), Bifunctional dihydrofolate reductase-thymidylate synthase (100786311), Bifunctional dihydrofolate reductase-thymidylate synthase
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei30Substrate; via carbonyl oxygenBy similarity1
Binding sitei32NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei52SubstrateBy similarity1
Binding sitei139Substrate; via carbonyl oxygenBy similarity1
Binding sitei160SubstrateBy similarity1
Binding sitei267dUMPBy similarity1
Active sitei412By similarity1
Binding sitei413dUMPBy similarity1
Binding sitei443dUMPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 44NADPBy similarity7
Nucleotide bindingi76 – 78NADPBy similarity3
Nucleotide bindingi97 – 100NADPBy similarity4
Nucleotide bindingi140 – 147NADPBy similarity8
Nucleotide bindingi431 – 435dUMPBy similarity5
Nucleotide bindingi473 – 475dUMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Multifunctional enzyme, Oxidoreductase, Transferase
Biological processNucleotide biosynthesis, One-carbon metabolism
LigandNADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-9361
UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthase
Short name:
DHFR-TS
Including the following 2 domains:
Dihydrofolate reductase (EC:1.5.1.3)
Thymidylate synthase (EC:2.1.1.45)
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
Proteomesi
  • UP000008827 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863591 – 530Bifunctional dihydrofolate reductase-thymidylate synthaseAdd BLAST530

Proteomic databases

PRIDEiP51820

Interactioni

Protein-protein interaction databases

STRINGi3847.GLYMA06G01280.3

Structurei

3D structure databases

ProteinModelPortaliP51820
SMRiP51820
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 203DHFRAdd BLAST178

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni206 – 530Thymidylate synthaseAdd BLAST325

Sequence similaritiesi

In the N-terminal section; belongs to the dihydrofolate reductase family.Curated
In the C-terminal section; belongs to the thymidylate synthase family.Curated

Phylogenomic databases

eggNOGiENOG410JUW5 Eukaryota
KOG0673 Eukaryota
KOG1324 Eukaryota
COG0207 LUCA
COG0262 LUCA
KOiK13998

Family and domain databases

CDDicd00209 DHFR, 1 hit
cd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
3.40.430.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR024072 DHFR-like_dom_sf
IPR012262 DHFR-TS
IPR017925 DHFR_CS
IPR001796 DHFR_dom
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PANTHERiPTHR11548:SF7 PTHR11548:SF7, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
PF00303 Thymidylat_synt, 1 hit
PIRSFiPIRSF000389 DHFR-TS, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF53597 SSF53597, 1 hit
SSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

P51820-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSDSSVISN GHSNGSVNPL PNLQRTYQVV VAATQDWGIG KDGKLPWRLP
60 70 80 90 100
TDLKFFKEIT MKTSEPGKKN AIVMGRKTWE SIPLEYRPLS GRLNVVLTRS
110 120 130 140 150
GSFDIATAEN VVICGSMSSA LELLAASPYS LSIEKVFVIG GGQIFREALN
160 170 180 190 200
VPGCEAIHLT EIQSSIECDT FMPPVDFTIF RPWYSSFPKV ENNIRYSFTT
210 220 230 240 250
YVRVRSSAAE SAGQNIDPLL DNNSESMKFE VKDFSFLPKM ISERHEEYLY
260 270 280 290 300
LKLVQDIIAE GTTKGDRTGT GTLSKFGCQM RFNLRGNFPL LTTKKVFWRG
310 320 330 340 350
VVEELLWFIS GSTNAKVLQE KGIHIWDGNA SREYLDGVGL TEREEGDLGP
360 370 380 390 400
VYGFQWRHFG ARYTDMHHDY SGQGFDQLLD VINKIKRNPD DRRIILSAWN
410 420 430 440 450
PVDLKLMALP PCHMFAQFYV AHGELSCQMY QRSADMGLGI PFNIASYALL
460 470 480 490 500
TCMIAHVCDL IPGDFIHVIG DAHIYRNHVR PLQEQLHNQP KPFPTLKINP
510 520 530
KKKDIDSFVA ADFKLIGYDP HQKIDMKLSV
Length:530
Mass (Da):59,745
Last modified:October 1, 1996 - v1
Checksum:iAFD710709FD5D820
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S78087 mRNA Translation: AAB34317.1
PIRiS55683
RefSeqiNP_001238644.1, NM_001251715.1
UniGeneiGma.483

Genome annotation databases

GeneIDi547833
KEGGigmx:547833

Similar proteinsi

Entry informationi

Entry nameiDRTS_SOYBN
AccessioniPrimary (citable) accession number: P51820
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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