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Protein

Heat shock protein 90-3

Gene

HSP90-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a holding molecular chaperone (holdase) which stabilizes unfolding protein intermediates and rapidly releases them in an active form once stress has abated. Functions as a folding molecular chaperone (foldase) that assists the non-covalent folding of proteins in an ATP-dependent manner (PubMed:23827697). Regulates RPP4-mediated temperature-dependent cell death and defense responses (PubMed:24611624). May assist SGT1B in the formation of SCF E3 ubiquitin ligase complexes that target the immune receptors SNC1, RPS2 and RPS4 for degradation, to regulate receptor levels and avoid autoimmunity (PubMed:24889324).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34ATPBy similarity1
Binding sitei38ATPBy similarity1
Binding sitei80ATPBy similarity1
Binding sitei85ATPBy similarity1
Binding sitei93ATPBy similarity1
Binding sitei99ATPBy similarity1
Binding sitei172ATPBy similarity1
Binding sitei371ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi100 – 101ATPBy similarity2
Nucleotide bindingi120 – 125ATPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processImmunity, Innate immunity, Plant defense, Stress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-ATH-3371571 HSF1-dependent transactivation
R-ATH-6798695 Neutrophil degranulation
R-ATH-844456 The NLRP3 inflammasome

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock protein 90-3Curated
Short name:
AtHSP90.3Curated
Short name:
AtHsp90-31 Publication
Alternative name(s):
HSP81.2
Heat shock protein 81-3Curated
Short name:
Hsp81-31 Publication
Protein MUTANT SNC1-ENHANCING 101 Publication
Gene namesi
Name:HSP90-31 Publication
Synonyms:HSP81-31 Publication, HSP81.2, MUSE101 Publication
Ordered Locus Names:At5g56010
ORF Names:MDA7.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G56010
TAIRilocus:2161790 AT5G56010

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi100S → F in muse10; enhances snc1-mediated autoimmune phenotypes. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000629481 – 699Heat shock protein 90-3Add BLAST699

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei219PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP51818
PRIDEiP51818

PTM databases

iPTMnetiP51818

Expressioni

Tissue specificityi

Present in all tissues. Most abundantly expressed in roots and floral bud clusters followed by flowers, young fruits and rosette leaves.

Developmental stagei

Expressed in pollen during pollen development, germination and tube growth. Expressed during embryo development and young seedling growth.1 Publication

Inductioni

In contrast to other major heat shock proteins, this one is also expressed at normal growth temperatures. Levels increase only slightly after heat shock. Also increase after salt treatment.

Gene expression databases

ExpressionAtlasiP51818 baseline and differential
GenevisibleiP51818 AT

Interactioni

Subunit structurei

Homodimer. Interacts with OEP61, OEP64 and OM64 (PubMed:24036116). Interacts with SNC1 (PubMed:24889324).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi20943, 7 interactors
STRINGi3702.AT5G56010.1

Structurei

3D structure databases

ProteinModelPortaliP51818
SMRiP51818
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi695 – 699TPR repeat-binding5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi222 – 226Poly-Glu5
Compositional biasi246 – 252Poly-Lys7
Compositional biasi529 – 532Poly-Lys4

Domaini

The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.By similarity

Sequence similaritiesi

Belongs to the heat shock protein 90 family.Curated

Phylogenomic databases

eggNOGiKOG0019 Eukaryota
COG0326 LUCA
HOGENOMiHOG000031988
InParanoidiP51818
KOiK04079
OMAiENTIMDE
OrthoDBiEOG093604DD
PhylomeDBiP51818

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di1.20.120.790, 1 hit
3.30.565.10, 1 hit
HAMAPiMF_00505 HSP90, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR019805 Heat_shock_protein_90_CS
IPR037196 HSP90_C
IPR001404 Hsp90_fam
IPR020575 Hsp90_N
IPR020568 Ribosomal_S5_D2-typ_fold
PANTHERiPTHR11528 PTHR11528, 1 hit
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00183 HSP90, 1 hit
PIRSFiPIRSF002583 Hsp90, 1 hit
PRINTSiPR00775 HEATSHOCK90
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF110942 SSF110942, 1 hit
SSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS00298 HSP90, 1 hit

Sequencei

Sequence statusi: Complete.

P51818-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADAETFAFQ AEINQLLSLI INTFYSNKEI FLRELISNSS DALDKIRFES
60 70 80 90 100
LTDKSKLDGQ PELFIHIIPD KTNNTLTIID SGIGMTKADL VNNLGTIARS
110 120 130 140 150
GTKEFMEALA AGADVSMIGQ FGVGFYSAYL VADKVVVTTK HNDDEQYVWE
160 170 180 190 200
SQAGGSFTVT RDTSGEALGR GTKMVLYLKE DQMEYIEERR LKDLVKKHSE
210 220 230 240 250
FISYPISLWI EKTIEKEISD DEEEEEKKDE EGKVEEVDEE KEKEEKKKKK
260 270 280 290 300
IKEVSHEWDL VNKQKPIWMR KPEEINKEEY AAFYKSLSND WEEHLAVKHF
310 320 330 340 350
SVEGQLEFKA ILFVPKRAPF DLFDTKKKPN NIKLYVRRVF IMDNCEDIIP
360 370 380 390 400
EYLGFVKGIV DSEDLPLNIS RETLQQNKIL KVIRKNLVKK CLELFFEIAE
410 420 430 440 450
NKEDYNKFYE AFSKNLKLGI HEDSQNRTKI AELLRYHSTK SGDELTSLKD
460 470 480 490 500
YVTRMKEGQN DIFYITGESK KAVENSPFLE KLKKKGIEVL YMVDAIDEYA
510 520 530 540 550
IGQLKEFEGK KLVSATKEGL KLDETEDEKK KKEELKEKFE GLCKVIKDVL
560 570 580 590 600
GDKVEKVIVS DRVVDSPCCL VTGEYGWTAN MERIMKAQAL RDSSMGGYMS
610 620 630 640 650
SKKTMEINPE NSIMDELRKR ADADKNDKSV KDLVLLLFET ALLTSGFSLD
660 670 680 690
EPNTFGSRIH RMLKLGLSID DDDVVEADAD MPPLEDDADA EGSKMEEVD
Length:699
Mass (Da):80,052
Last modified:December 6, 2002 - v2
Checksum:i1DC8044D787665B6
GO

Sequence cautioni

The sequence AAB33937 differs from that shown. Sequencing errors.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28K → L in CAA72513 (PubMed:9426614).Curated1
Sequence conflicti92N → K in CAA72513 (PubMed:9426614).Curated1
Sequence conflicti482L → F in AAL49788 (PubMed:14593172).Curated1
Sequence conflicti498E → G in CAA72513 (PubMed:9426614).Curated1
Sequence conflicti549V → L in CAA72513 (PubMed:9426614).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S77849 Genomic DNA Translation: AAB33937.1 Sequence problems.
Y11827 Genomic DNA Translation: CAA72513.1
AB011476 Genomic DNA Translation: BAB09283.1
CP002688 Genomic DNA Translation: AED96709.1
AY062832 mRNA Translation: AAL32910.1
AY081302 mRNA Translation: AAL91191.1
AY070031 mRNA Translation: AAL49788.1
AY050349 mRNA Translation: AAK91366.1
RefSeqiNP_200412.1, NM_124983.4
UniGeneiAt.45945
At.47215

Genome annotation databases

EnsemblPlantsiAT5G56010.1; AT5G56010.1; AT5G56010
GeneIDi835699
GrameneiAT5G56010.1; AT5G56010.1; AT5G56010
KEGGiath:AT5G56010

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S77849 Genomic DNA Translation: AAB33937.1 Sequence problems.
Y11827 Genomic DNA Translation: CAA72513.1
AB011476 Genomic DNA Translation: BAB09283.1
CP002688 Genomic DNA Translation: AED96709.1
AY062832 mRNA Translation: AAL32910.1
AY081302 mRNA Translation: AAL91191.1
AY070031 mRNA Translation: AAL49788.1
AY050349 mRNA Translation: AAK91366.1
RefSeqiNP_200412.1, NM_124983.4
UniGeneiAt.45945
At.47215

3D structure databases

ProteinModelPortaliP51818
SMRiP51818
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20943, 7 interactors
STRINGi3702.AT5G56010.1

PTM databases

iPTMnetiP51818

Proteomic databases

PaxDbiP51818
PRIDEiP51818

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G56010.1; AT5G56010.1; AT5G56010
GeneIDi835699
GrameneiAT5G56010.1; AT5G56010.1; AT5G56010
KEGGiath:AT5G56010

Organism-specific databases

AraportiAT5G56010
TAIRilocus:2161790 AT5G56010

Phylogenomic databases

eggNOGiKOG0019 Eukaryota
COG0326 LUCA
HOGENOMiHOG000031988
InParanoidiP51818
KOiK04079
OMAiENTIMDE
OrthoDBiEOG093604DD
PhylomeDBiP51818

Enzyme and pathway databases

ReactomeiR-ATH-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-ATH-3371571 HSF1-dependent transactivation
R-ATH-6798695 Neutrophil degranulation
R-ATH-844456 The NLRP3 inflammasome

Miscellaneous databases

PROiPR:P51818

Gene expression databases

ExpressionAtlasiP51818 baseline and differential
GenevisibleiP51818 AT

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di1.20.120.790, 1 hit
3.30.565.10, 1 hit
HAMAPiMF_00505 HSP90, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR019805 Heat_shock_protein_90_CS
IPR037196 HSP90_C
IPR001404 Hsp90_fam
IPR020575 Hsp90_N
IPR020568 Ribosomal_S5_D2-typ_fold
PANTHERiPTHR11528 PTHR11528, 1 hit
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00183 HSP90, 1 hit
PIRSFiPIRSF002583 Hsp90, 1 hit
PRINTSiPR00775 HEATSHOCK90
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF110942 SSF110942, 1 hit
SSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS00298 HSP90, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHS903_ARATH
AccessioniPrimary (citable) accession number: P51818
Secondary accession number(s): O03985
, Q8VYT8, Q94A60, Q9FKU5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 6, 2002
Last modified: October 10, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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Main funding by: National Institutes of Health

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