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Protein

Chloride channel protein ClC-Kb

Gene

Clcnkb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi259CalciumBy similarity1
Metal bindingi261CalciumBy similarity1
Metal bindingi278CalciumBy similarity1
Metal bindingi281CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChloride channel, Ion channel, Voltage-gated channel
Biological processIon transport, Transport
LigandCalcium, Chloride, Metal-binding

Protein family/group databases

TCDBi2.A.49.2.5 the chloride carrier/channel (clc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein ClC-Kb
Short name:
Chloride channel Kb
Alternative name(s):
ClC-K2
Gene namesi
Name:Clcnkb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628639 Clcnkb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 51CytoplasmicSequence analysisAdd BLAST51
Transmembranei52 – 72HelicalSequence analysisAdd BLAST21
Transmembranei94 – 114HelicalSequence analysisAdd BLAST21
Transmembranei161 – 181HelicalSequence analysisAdd BLAST21
Transmembranei202 – 222HelicalSequence analysisAdd BLAST21
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21
Transmembranei275 – 295HelicalSequence analysisAdd BLAST21
Transmembranei325 – 345HelicalSequence analysisAdd BLAST21
Transmembranei410 – 430HelicalSequence analysisAdd BLAST21
Transmembranei434 – 454HelicalSequence analysisAdd BLAST21
Transmembranei458 – 478HelicalSequence analysisAdd BLAST21
Transmembranei486 – 506HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944621 – 687Chloride channel protein ClC-KbAdd BLAST687

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi364N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP51802
PRIDEiP51802

PTM databases

PhosphoSitePlusiP51802

Expressioni

Tissue specificityi

Expressed predominantly in the kidney. Expressed in all segments of the nephron examined, including the S2 segment and the glomerulus.

Inductioni

Expression is consitently weaker in the absence of BSND protein expression than it is in its presence. The half-life with BSND protein is much longer than that without it. Rapidly degraded without BSND protein, exhibiting a very short half-life of less than 1 hour.1 Publication

Interactioni

Subunit structurei

Interacts with BSND.2 Publications

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013550

Structurei

3D structure databases

ProteinModelPortaliP51802
SMRiP51802
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini551 – 609CBS 1PROSITE-ProRule annotationAdd BLAST59
Domaini626 – 687CBS 2PROSITE-ProRule annotationAdd BLAST62

Sequence similaritiesi

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0476 Eukaryota
COG0038 LUCA
HOGENOMiHOG000231297
HOVERGENiHBG005332
InParanoidiP51802
KOiK05018
PhylomeDBiP51802

Family and domain databases

Gene3Di1.10.3080.10, 1 hit
InterProiView protein in InterPro
IPR000644 CBS_dom
IPR014743 Cl-channel_core
IPR001807 Cl-channel_volt-gated
IPR002250 Cl_channel-K
PfamiView protein in Pfam
PF00571 CBS, 1 hit
PF00654 Voltage_CLC, 1 hit
PRINTSiPR00762 CLCHANNEL
PR01119 CLCHANNELKDY
SMARTiView protein in SMART
SM00116 CBS, 1 hit
SUPFAMiSSF81340 SSF81340, 1 hit
PROSITEiView protein in PROSITE
PS51371 CBS, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P51802-1) [UniParc]FASTAAdd to basket
Also known as: ClC-K2L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEEIVGLREG SPRKPVPLQE LWRPCPRIRR NIQGSLEWLK ERLFRVGEDW
60 70 80 90 100
YFLVALGVLM ALISYAMNFA IGRVVRAHKW LYREIGDGHL LRYLSWTVYP
110 120 130 140 150
VALLSFSSGF SQSITPSSGG SGIPEVKTIL TGVILEDYLD IKNFGAKVVG
160 170 180 190 200
LSCTLATGST IFLGKLGPFV HLSVMIAAYL GRVRTKTVGE PENKTKEMEL
210 220 230 240 250
LAAGAAVGVA TVFAAPISGV LFSIEVMSSH FSVWDYWRGF FAATCGAFMF
260 270 280 290 300
HLLAVFNSEQ ETITSIYKTS FPVDIPFDLP EIFFFVALGA ICGILSCGYN
310 320 330 340 350
YCQRTSLFFL KSNGFTSKLL ATSKPLYSAL AAVVLASITY PPGVGRFMAS
360 370 380 390 400
RLSMSEYLET LFDNNSWALM TKNSSPPWSA EPDPQNLWLE WCHPQMTVFG
410 420 430 440 450
TLVFFLVMKF WMLILATTIP IPAGYFLPIF VYGAAIGRLF GEVLSLAFPE
460 470 480 490 500
GIVAGGKVSP IMPGAYALAG AAAFSGAVTH TLSTALLAFE VSGQIVHALP
510 520 530 540 550
VLMAVLAANA ICQSYQPSFY DGTIIVKKLP YLPWIRGRKI GSHLVTVGHF
560 570 580 590 600
MNCTLTTLAK DMPLEEVIKV VISTDVTQYP LVETTESQVL VGIVKRTHLV
610 620 630 640 650
QSLHTDSASW APGQQPCLQD ILANGCPTQP VTLQLSTETS LHETHNLFEL
660 670 680
LNLQLLFVTS RGRAVGSVSW VELKKAISTL TNPPAPK
Length:687
Mass (Da):75,218
Last modified:October 1, 1996 - v1
Checksum:i35B0BD7735397880
GO
Isoform 2 (identifier: P51802-2) [UniParc]FASTAAdd to basket
Also known as: ClC-K2S

The sequence of this isoform differs from the canonical sequence as follows:
     77-131: Missing.

Show »
Length:632
Mass (Da):69,211
Checksum:i7C62CDD5822EF0D6
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V7B3G3V7B3_RAT
Chloride channel protein
Clcnkb rCG_30632
687Annotation score:
E9PTA8E9PTA8_RAT
Chloride channel protein
Clcnkb
644Annotation score:
Q1W583Q1W583_RAT
Chloride channel protein ClC-Kb
Clcnkb Clck2
43Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00104977 – 131Missing in isoform 2. 1 PublicationAdd BLAST55

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z30663 mRNA Translation: CAA83143.1
D26111 mRNA Translation: BAA05106.1
D26111 mRNA Translation: BAA05107.1
PIRiB57713
RefSeqiNP_775126.1, NM_173103.1 [P51802-1]
UniGeneiRn.10339

Genome annotation databases

GeneIDi79430
KEGGirno:79430
UCSCiRGD:628639 rat [P51802-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z30663 mRNA Translation: CAA83143.1
D26111 mRNA Translation: BAA05106.1
D26111 mRNA Translation: BAA05107.1
PIRiB57713
RefSeqiNP_775126.1, NM_173103.1 [P51802-1]
UniGeneiRn.10339

3D structure databases

ProteinModelPortaliP51802
SMRiP51802
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013550

Protein family/group databases

TCDBi2.A.49.2.5 the chloride carrier/channel (clc) family

PTM databases

PhosphoSitePlusiP51802

Proteomic databases

PaxDbiP51802
PRIDEiP51802

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi79430
KEGGirno:79430
UCSCiRGD:628639 rat [P51802-1]

Organism-specific databases

CTDi1188
RGDi628639 Clcnkb

Phylogenomic databases

eggNOGiKOG0476 Eukaryota
COG0038 LUCA
HOGENOMiHOG000231297
HOVERGENiHBG005332
InParanoidiP51802
KOiK05018
PhylomeDBiP51802

Miscellaneous databases

PROiPR:P51802

Family and domain databases

Gene3Di1.10.3080.10, 1 hit
InterProiView protein in InterPro
IPR000644 CBS_dom
IPR014743 Cl-channel_core
IPR001807 Cl-channel_volt-gated
IPR002250 Cl_channel-K
PfamiView protein in Pfam
PF00571 CBS, 1 hit
PF00654 Voltage_CLC, 1 hit
PRINTSiPR00762 CLCHANNEL
PR01119 CLCHANNELKDY
SMARTiView protein in SMART
SM00116 CBS, 1 hit
SUPFAMiSSF81340 SSF81340, 1 hit
PROSITEiView protein in PROSITE
PS51371 CBS, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCLCKB_RAT
AccessioniPrimary (citable) accession number: P51802
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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