UniProtKB - P51796 (CLCN5_RAT)
Protein
H(+)/Cl(-) exchange transporter 5
Gene
Clcn5
Organism
Rattus norvegicus (Rat)
Status
Functioni
Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function (By similarity).By similarity1 Publication
Miscellaneous
The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 238 | ChlorideBy similarity | 1 | |
Sitei | 281 | Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transportBy similarity | 1 | |
Sitei | 338 | Mediates proton transfer from the protein to the inner aqueous phaseBy similarity | 1 | |
Binding sitei | 525 | Chloride; via amide nitrogenBy similarity | 1 | |
Binding sitei | 628 | ChlorideBy similarity | 1 | |
Binding sitei | 666 | ATP; via amide nitrogen and carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 687 – 689 | ATPBy similarity | 3 | |
Nucleotide bindingi | 794 – 797 | ATPBy similarity | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- identical protein binding Source: RGD
- solute:proton antiporter activity Source: GO_Central
- voltage-gated chloride channel activity Source: RGD
GO - Biological processi
- chloride transport Source: RGD
- endocytosis Source: RGD
Keywordsi
Biological process | Antiport, Ion transport, Transport |
Ligand | ATP-binding, Chloride, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-2672351, Stimuli-sensing channels |
Names & Taxonomyi
Protein namesi | Recommended name: H(+)/Cl(-) exchange transporter 5Alternative name(s): Chloride channel protein 5 Short name: ClC-5 Chloride transporter ClC-5 |
Gene namesi | Name:Clcn5 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2362, Clcn5 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Multi-pass membrane protein By similarity
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Endosome
- Endosome membrane By similarity; Multi-pass membrane protein By similarity
Endosome
- early endosome Source: GO_Central
- endosome Source: RGD
- endosome membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: GO_Central
- Golgi membrane Source: UniProtKB-SubCell
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
Other locations
- apical part of cell Source: UniProtKB
- membrane Source: UniProtKB
- synaptic vesicle Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 124 | CytoplasmicBy similarityAdd BLAST | 124 | |
Transmembranei | 125 – 162 | HelicalBy similarityAdd BLAST | 38 | |
Transmembranei | 208 – 231 | HelicalBy similarityAdd BLAST | 24 | |
Intramembranei | 240 – 247 | HelicalBy similarity | 8 | |
Transmembranei | 256 – 275 | HelicalBy similarityAdd BLAST | 20 | |
Transmembranei | 281 – 300 | HelicalBy similarityAdd BLAST | 20 | |
Intramembranei | 312 – 324 | HelicalBy similarityAdd BLAST | 13 | |
Intramembranei | 328 – 336 | HelicalBy similarity | 9 | |
Transmembranei | 348 – 366 | HelicalBy similarityAdd BLAST | 19 | |
Transmembranei | 389 – 414 | HelicalBy similarityAdd BLAST | 26 | |
Transmembranei | 422 – 442 | HelicalBy similarityAdd BLAST | 21 | |
Transmembranei | 498 – 518 | HelicalBy similarityAdd BLAST | 21 | |
Transmembranei | 523 – 542 | HelicalBy similarityAdd BLAST | 20 | |
Intramembranei | 570 – 584 | HelicalBy similarityAdd BLAST | 15 | |
Intramembranei | 585 – 587 | Note=Loop between two helicesBy similarity | 3 | |
Intramembranei | 588 – 599 | HelicalBy similarityAdd BLAST | 12 | |
Intramembranei | 600 – 604 | Note=Loop between two helicesBy similarity | 5 | |
Transmembranei | 605 – 622 | HelicalBy similarityAdd BLAST | 18 | |
Topological domaini | 623 – 816 | CytoplasmicBy similarityAdd BLAST | 194 |
Keywords - Cellular componenti
Cell membrane, Endosome, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000094448 | 1 – 816 | H(+)/Cl(-) exchange transporter 5Add BLAST | 816 |
Post-translational modificationi
Ubiquitinated by NEDD4L in the presence of albumin; which promotes endocytosis and proteasomal degradation.By similarity
Keywords - PTMi
Ubl conjugationProteomic databases
PaxDbi | P51796 |
PTM databases
PhosphoSitePlusi | P51796 |
Expressioni
Tissue specificityi
Kidney specific.
Gene expression databases
Bgeei | ENSRNOG00000002862, Expressed in adult mammalian kidney and 19 other tissues |
ExpressionAtlasi | P51796, baseline and differential |
Genevisiblei | P51796, RN |
Interactioni
Subunit structurei
Interacts with NEDD4 and NEDD4L.
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000003895 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 656 – 720 | CBS 1PROSITE-ProRule annotationAdd BLAST | 65 | |
Domaini | 752 – 811 | CBS 2PROSITE-ProRule annotationAdd BLAST | 60 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 237 – 241 | Selectivity filter part_1By similarity | 5 | |
Motifi | 279 – 283 | Selectivity filter part_2By similarity | 5 | |
Motifi | 523 – 527 | Selectivity filter part_3By similarity | 5 |
Sequence similaritiesi
Belongs to the chloride channel (TC 2.A.49) family. ClC-5/CLCN5 subfamily. [View classification]Curated
Keywords - Domaini
CBS domain, Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0475, Eukaryota |
GeneTreei | ENSGT00940000153763 |
HOGENOMi | CLU_003181_2_1_1 |
InParanoidi | P51796 |
OMAi | ACCCANL |
OrthoDBi | 271925at2759 |
PhylomeDBi | P51796 |
Family and domain databases
Gene3Di | 1.10.3080.10, 1 hit |
InterProi | View protein in InterPro IPR000644, CBS_dom IPR014743, Cl-channel_core IPR001807, Cl-channel_volt-gated IPR002247, Cl_channel-5 |
Pfami | View protein in Pfam PF00571, CBS, 2 hits PF00654, Voltage_CLC, 1 hit |
PRINTSi | PR00762, CLCHANNEL PR01116, CLCHANNEL5 |
SMARTi | View protein in SMART SM00116, CBS, 2 hits |
SUPFAMi | SSF81340, SSF81340, 1 hit |
PROSITEi | View protein in PROSITE PS51371, CBS, 2 hits |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: P51796-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAMWQGAMDN RGFHQGSFSS FQSSSSDEDL MDIPGTAMDF SMRDDVPPLD
60 70 80 90 100
REIEGNKSYN GGGIGSSNRI MDFLEEPIPG VGTYDDFNTI DWVREKSRDR
110 120 130 140 150
DRHREITNKS KESTWALIHS VSDAFSGWLL MLLIGLLSGS LAGLIDISAH
160 170 180 190 200
WMTDLKEGIC TGGFWFNHEH CCWNSEHVTF EDRDKCPEWN SWSQLIISTD
210 220 230 240 250
QGAFAYIVNY FMYVLWALLF AFLAVSLVKA FAPYACGSGI PEIKTILSGF
260 270 280 290 300
IIRGYLGKWT LVIKTITLVL AVSSGLSLGK EGPLVHVACC CGNILCHCFN
310 320 330 340 350
KYRKNEAKRR EVLSAAAAAG VSVAFGAPIG GVLFSLEEVS YYFPLKTLWR
360 370 380 390 400
SFFAALVAAF TLRSINPFGN SRLVLFYVEF HTPWHLFELV PFIVLGIFGG
410 420 430 440 450
LWGALFIRTN IAWCRKRKTT QLGKYPVVEV LIVTAITAIL AFPNEYTRMS
460 470 480 490 500
TSELISELFN DCGLLDSSKL CDYENHFNTS KGGELPDRPA GVGVYSAMWQ
510 520 530 540 550
LALTLILKIV ITIFTFGMKI PSGLFIPSMA VGAIAGRLLG VGMEQLAYYH
560 570 580 590 600
HDWGIFNSWC SQGADCITPG LYAMVGAAAC LGGVTRMTVS LVVIMFELTG
610 620 630 640 650
GLEYIVPLMA AAMTSKWVAD ALGREGIYDA HIRLNGYPFL EAKEEFAHKT
660 670 680 690 700
LAMDVMKPRR NDPLLTVLTQ DSMTVEDVET IISETTYSGF PVVVSRESQR
710 720 730 740 750
LVGFVLRRDL IISIENARKK QDGVVSTSII YFTEHSPPMP PYTPPTLKLR
760 770 780 790 800
NILDLSPFTV TDLTPMEIVV DIFRKLGLRQ CLVTHNGRLL GIITKKDVLK
810
HIAQMANQDP ESILFN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 385 | H → Y in BAA09091 (PubMed:8626585).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_060659 | 1 – 70 | Missing in isoform 2. CuratedAdd BLAST | 70 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z56277 mRNA Translation: CAA91216.1 D50497 mRNA Translation: BAA09091.1 AABR07037207 Genomic DNA No translation available. AABR07037208 Genomic DNA No translation available. |
RefSeqi | NP_058802.1, NM_017106.1 [P51796-2] |
Genome annotation databases
Ensembli | ENSRNOT00000003895; ENSRNOP00000003895; ENSRNOG00000002862 [P51796-2] ENSRNOT00000079054; ENSRNOP00000074459; ENSRNOG00000002862 [P51796-1] |
GeneIDi | 25749 |
KEGGi | rno:25749 |
UCSCi | RGD:2362, rat [P51796-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z56277 mRNA Translation: CAA91216.1 D50497 mRNA Translation: BAA09091.1 AABR07037207 Genomic DNA No translation available. AABR07037208 Genomic DNA No translation available. |
RefSeqi | NP_058802.1, NM_017106.1 [P51796-2] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000003895 |
PTM databases
PhosphoSitePlusi | P51796 |
Proteomic databases
PaxDbi | P51796 |
Protocols and materials databases
ABCDi | P51796, 2 sequenced antibodies |
Genome annotation databases
Ensembli | ENSRNOT00000003895; ENSRNOP00000003895; ENSRNOG00000002862 [P51796-2] ENSRNOT00000079054; ENSRNOP00000074459; ENSRNOG00000002862 [P51796-1] |
GeneIDi | 25749 |
KEGGi | rno:25749 |
UCSCi | RGD:2362, rat [P51796-1] |
Organism-specific databases
CTDi | 1184 |
RGDi | 2362, Clcn5 |
Phylogenomic databases
eggNOGi | KOG0475, Eukaryota |
GeneTreei | ENSGT00940000153763 |
HOGENOMi | CLU_003181_2_1_1 |
InParanoidi | P51796 |
OMAi | ACCCANL |
OrthoDBi | 271925at2759 |
PhylomeDBi | P51796 |
Enzyme and pathway databases
Reactomei | R-RNO-2672351, Stimuli-sensing channels |
Miscellaneous databases
PROi | PR:P51796 |
Gene expression databases
Bgeei | ENSRNOG00000002862, Expressed in adult mammalian kidney and 19 other tissues |
ExpressionAtlasi | P51796, baseline and differential |
Genevisiblei | P51796, RN |
Family and domain databases
Gene3Di | 1.10.3080.10, 1 hit |
InterProi | View protein in InterPro IPR000644, CBS_dom IPR014743, Cl-channel_core IPR001807, Cl-channel_volt-gated IPR002247, Cl_channel-5 |
Pfami | View protein in Pfam PF00571, CBS, 2 hits PF00654, Voltage_CLC, 1 hit |
PRINTSi | PR00762, CLCHANNEL PR01116, CLCHANNEL5 |
SMARTi | View protein in SMART SM00116, CBS, 2 hits |
SUPFAMi | SSF81340, SSF81340, 1 hit |
PROSITEi | View protein in PROSITE PS51371, CBS, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CLCN5_RAT | |
Accessioni | P51796Primary (citable) accession number: P51796 Secondary accession number(s): A0A0G2K839, P70642 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | August 12, 2020 | |
Last modified: | April 7, 2021 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families