UniProtKB - P51693 (APLP1_HUMAN)
Amyloid-like protein 1
APLP1
Functioni
Miscellaneous
GO - Molecular functioni
- alpha-2A adrenergic receptor binding Source: BHF-UCL
- alpha-2B adrenergic receptor binding Source: BHF-UCL
- alpha-2C adrenergic receptor binding Source: BHF-UCL
- heparin binding Source: UniProtKB-KW
- identical protein binding Source: IntAct
- transition metal ion binding Source: InterPro
GO - Biological processi
- animal organ morphogenesis Source: ProtInc
- apoptotic process Source: UniProtKB-KW
- cell adhesion Source: UniProtKB-KW
- cellular response to norepinephrine stimulus Source: BHF-UCL
- endocytosis Source: UniProtKB-KW
- extracellular matrix organization Source: ARUK-UCL
- forebrain development Source: ARUK-UCL
- negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: BHF-UCL
- nervous system development Source: ProtInc
Keywordsi
Molecular function | Heparin-binding |
Biological process | Apoptosis, Cell adhesion, Endocytosis |
Ligand | Copper, Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | P51693 |
Names & Taxonomyi
Protein namesi | Recommended name: Amyloid-like protein 1Short name: APLP Short name: APLP-1 Cleaved into the following chain: |
Gene namesi | Name:APLP1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:597, APLP1 |
MIMi | 104775, gene |
neXtProti | NX_P51693 |
VEuPathDBi | HostDB:ENSG00000105290.11 |
Subcellular locationi
Plasma membrane
Extracellular region or secreted
- basement membrane Source: ProtInc
Plasma Membrane
- plasma membrane Source: BHF-UCL
Other locations
- integral component of membrane Source: UniProtKB-KW
- perinuclear region of cytoplasm Source: BHF-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 39 – 580 | ExtracellularSequence analysisAdd BLAST | 542 | |
Transmembranei | 581 – 603 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 604 – 650 | CytoplasmicSequence analysisAdd BLAST | 47 |
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 426 | H → A: Reduced affinity for heparin. Reduces homodimerization. 1 Publication | 1 | |
Mutagenesisi | 429 | R → A: Strongly reduced affinity for heparin. Strongly reduced homodimerization. 1 Publication | 1 | |
Mutagenesisi | 433 | H → A: Reduced affinity for heparin. Reduces homodimerization. 1 Publication | 1 |
Keywords - Diseasei
NeurodegenerationOrganism-specific databases
DisGeNETi | 333 |
OpenTargetsi | ENSG00000105290 |
PharmGKBi | PA24884 |
Miscellaneous databases
Pharosi | P51693, Tbio |
Chemistry databases
DrugBanki | DB09130, Copper DB01593, Zinc DB14487, Zinc acetate DB14533, Zinc chloride DB14548, Zinc sulfate, unspecified form |
Genetic variation databases
BioMutai | APLP1 |
DMDMi | 28558769 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 38 | Sequence analysisAdd BLAST | 38 | |
ChainiPRO_0000000203 | 39 – 650 | Amyloid-like protein 1Add BLAST | 612 | |
PeptideiPRO_0000000204 | 621 – 650 | C30By similarityAdd BLAST | 30 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 60 ↔ 84 | PROSITE-ProRule annotation | ||
Disulfide bondi | 95 ↔ 140 | PROSITE-ProRule annotation | ||
Disulfide bondi | 120 ↔ 128 | PROSITE-ProRule annotation | ||
Disulfide bondi | 156 ↔ 210 | PROSITE-ProRule annotation | ||
Disulfide bondi | 167 ↔ 197 | PROSITE-ProRule annotation | ||
Disulfide bondi | 181 ↔ 209 | PROSITE-ProRule annotation | ||
Glycosylationi | 215 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 227 | O-linked (GalNAc...) serine1 Publication | 1 | |
Glycosylationi | 228 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 337 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 461 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 551 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 620 – 621 | Cleavage; by caspase-3By similarity | 2 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MassIVEi | P51693 |
PaxDbi | P51693 |
PeptideAtlasi | P51693 |
PRIDEi | P51693 |
ProteomicsDBi | 56379 [P51693-1] 56380 [P51693-2] |
TopDownProteomicsi | P51693-2 [P51693-2] |
PTM databases
GlyConnecti | 716, 1 O-Linked glycan (1 site) |
GlyGeni | P51693, 7 sites, 1 O-linked glycan (2 sites) |
iPTMneti | P51693 |
PhosphoSitePlusi | P51693 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000105290, Expressed in C1 segment of cervical spinal cord and 168 other tissues |
ExpressionAtlasi | P51693, baseline and differential |
Genevisiblei | P51693, HS |
Organism-specific databases
HPAi | ENSG00000105290, Tissue enriched (brain) |
Interactioni
Subunit structurei
Monomer and homodimer. Heparin binding promotes homodimerization. Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB and APBA family members, MAPK8IP1 and Dab1 (By similarity). Binding to Dab1 inhibits its serine phosphorylation (By similarity).
Interacts with CPEB1.
Interacts (via NPXY motif) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif.
Interacts (via NPXY motif) with DAB1 (By similarity).
By similarityBinary interactionsi
Hide detailsP51693
GO - Molecular functioni
- alpha-2A adrenergic receptor binding Source: BHF-UCL
- alpha-2B adrenergic receptor binding Source: BHF-UCL
- alpha-2C adrenergic receptor binding Source: BHF-UCL
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 106830, 51 interactors |
DIPi | DIP-30846N |
IntActi | P51693, 48 interactors |
MINTi | P51693 |
STRINGi | 9606.ENSP00000221891 |
Miscellaneous databases
RNActi | P51693, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P51693 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P51693 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 50 – 212 | E1PROSITE-ProRule annotationAdd BLAST | 163 | |
Domaini | 293 – 484 | E2PROSITE-ProRule annotationAdd BLAST | 192 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 50 – 146 | GFLD subdomainPROSITE-ProRule annotationAdd BLAST | 97 | |
Regioni | 154 – 212 | CuBD subdomainPROSITE-ProRule annotationAdd BLAST | 59 | |
Regioni | 158 – 178 | Copper-bindingBy similarityAdd BLAST | 21 | |
Regioni | 204 – 211 | Zinc-binding | 8 | |
Regioni | 285 – 305 | O-glycosylated at three sitesAdd BLAST | 21 | |
Regioni | 310 – 342 | Heparin-bindingBy similarityAdd BLAST | 33 | |
Regioni | 410 – 441 | Heparin-bindingBy similarityAdd BLAST | 32 | |
Regioni | 442 – 459 | Collagen-bindingBy similarityAdd BLAST | 18 | |
Regioni | 632 – 649 | Interaction with DAB1By similarityAdd BLAST | 18 | |
Regioni | 636 – 650 | Interaction with DAB2By similarityAdd BLAST | 15 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 604 – 615 | Basolateral sorting signalBy similarityAdd BLAST | 12 | |
Motifi | 640 – 643 | Clathrin-bindingSequence analysis | 4 | |
Motifi | 640 – 643 | NPXY motif; contains endocytosis signal | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 241 – 247 | Poly-Glu | 7 | |
Compositional biasi | 264 – 268 | Poly-Glu | 5 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3540, Eukaryota |
GeneTreei | ENSGT00530000063252 |
InParanoidi | P51693 |
OMAi | PELHIAR |
OrthoDBi | 953529at2759 |
PhylomeDBi | P51693 |
TreeFami | TF317274 |
Family and domain databases
Gene3Di | 1.20.120.770, 1 hit 3.30.1490.140, 1 hit 3.90.570.10, 1 hit |
InterProi | View protein in InterPro IPR036669, Amyloid_Cu-bd_sf IPR008155, Amyloid_glyco IPR011178, Amyloid_glyco_Cu-bd IPR024329, Amyloid_glyco_E2_domain IPR008154, Amyloid_glyco_extra IPR015849, Amyloid_glyco_heparin-bd IPR036454, Amyloid_glyco_heparin-bd_sf IPR019745, Amyloid_glyco_intracell_CS IPR019543, APP_amyloid_C IPR019744, APP_CUBD_CS IPR036176, E2_sf |
PANTHERi | PTHR23103, PTHR23103, 1 hit |
Pfami | View protein in Pfam PF10515, APP_amyloid, 1 hit PF12924, APP_Cu_bd, 1 hit PF12925, APP_E2, 1 hit PF02177, APP_N, 1 hit |
PRINTSi | PR00203, AMYLOIDA4 |
SMARTi | View protein in SMART SM00006, A4_EXTRA, 1 hit |
SUPFAMi | SSF109843, SSF109843, 1 hit SSF56491, SSF56491, 1 hit SSF89811, SSF89811, 1 hit |
PROSITEi | View protein in PROSITE PS00319, APP_CUBD, 1 hit PS51869, APP_E1, 1 hit PS51870, APP_E2, 1 hit PS00320, APP_INTRA, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGPASPAARG LSRRPGQPPL PLLLPLLLLL LRAQPAIGSL AGGSPGAAEA
60 70 80 90 100
PGSAQVAGLC GRLTLHRDLR TGRWEPDPQR SRRCLRDPQR VLEYCRQMYP
110 120 130 140 150
ELQIARVEQA TQAIPMERWC GGSRSGSCAH PHHQVVPFRC LPGEFVSEAL
160 170 180 190 200
LVPEGCRFLH QERMDQCESS TRRHQEAQEA CSSQGLILHG SGMLLPCGSD
210 220 230 240 250
RFRGVEYVCC PPPGTPDPSG TAVGDPSTRS WPPGSRVEGA EDEEEEESFP
260 270 280 290 300
QPVDDYFVEP PQAEEEEETV PPPSSHTLAV VGKVTPTPRP TDGVDIYFGM
310 320 330 340 350
PGEISEHEGF LRAKMDLEER RMRQINEVMR EWAMADNQSK NLPKADRQAL
360 370 380 390 400
NEHFQSILQT LEEQVSGERQ RLVETHATRV IALINDQRRA ALEGFLAALQ
410 420 430 440 450
ADPPQAERVL LALRRYLRAE QKEQRHTLRH YQHVAAVDPE KAQQMRFQVH
460 470 480 490 500
THLQVIEERV NQSLGLLDQN PHLAQELRPQ IQELLHSEHL GPSELEAPAP
510 520 530 540 550
GGSSEDKGGL QPPDSKDDTP MTLPKGSTEQ DAASPEKEKM NPLEQYERKV
560 570 580 590 600
NASVPRGFPF HSSEIQRDEL APAGTGVSRE AVSGLLIMGA GGGSLIVLSM
610 620 630 640 650
LLLRRKKPYG AISHGVVEVD PMLTLEEQQL RELQRHGYEN PTYRFLEERP
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5GZ08 | F5GZ08_HUMAN | Amyloid-like protein 1 | APLP1 | 611 | Annotation score: | ||
B7Z4G8 | B7Z4G8_HUMAN | Amyloid-like protein 1 | APLP1 | 644 | Annotation score: | ||
K7EQJ4 | K7EQJ4_HUMAN | Amyloid-like protein 1 | APLP1 | 294 | Annotation score: | ||
K7ELK0 | K7ELK0_HUMAN | Amyloid-like protein 1 | APLP1 | 173 | Annotation score: | ||
S4R3U6 | S4R3U6_HUMAN | Amyloid-like protein 1 | APLP1 | 289 | Annotation score: | ||
K7EMN4 | K7EMN4_HUMAN | Amyloid-like protein 1 | APLP1 | 221 | Annotation score: | ||
K7EMS1 | K7EMS1_HUMAN | Amyloid-like protein 1 | APLP1 | 118 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 48 | A → P in AAB96331 (PubMed:9428684).Curated | 1 | |
Sequence conflicti | 78 | P → S in BC013850 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_039100 | 517 | D → DA in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U48437 mRNA Translation: AAB96331.1 AD000864 Genomic DNA Translation: AAB50173.1 BC012889 mRNA Translation: AAH12889.1 BC013850 mRNA No translation available. |
CCDSi | CCDS32997.1 [P51693-2] |
RefSeqi | NP_001019978.1, NM_001024807.2 [P51693-2] NP_005157.1, NM_005166.4 [P51693-1] |
Genome annotation databases
Ensembli | ENST00000221891; ENSP00000221891; ENSG00000105290 [P51693-2] ENST00000652533; ENSP00000498366; ENSG00000105290 [P51693-1] |
GeneIDi | 333 |
KEGGi | hsa:333 |
UCSCi | uc002ocf.4, human [P51693-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U48437 mRNA Translation: AAB96331.1 AD000864 Genomic DNA Translation: AAB50173.1 BC012889 mRNA Translation: AAH12889.1 BC013850 mRNA No translation available. |
CCDSi | CCDS32997.1 [P51693-2] |
RefSeqi | NP_001019978.1, NM_001024807.2 [P51693-2] NP_005157.1, NM_005166.4 [P51693-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3PMR | X-ray | 2.11 | A/B | 285-499 | [»] | |
3Q7G | X-ray | 2.30 | A/B | 285-494 | [»] | |
3Q7L | X-ray | 2.20 | A/B | 285-494 | [»] | |
3QMK | X-ray | 2.21 | A/B | 285-494 | [»] | |
4RD9 | X-ray | 2.60 | A/B | 292-494 | [»] | |
4RDA | X-ray | 2.50 | A/B | 290-495 | [»] | |
SMRi | P51693 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 106830, 51 interactors |
DIPi | DIP-30846N |
IntActi | P51693, 48 interactors |
MINTi | P51693 |
STRINGi | 9606.ENSP00000221891 |
Chemistry databases
DrugBanki | DB09130, Copper DB01593, Zinc DB14487, Zinc acetate DB14533, Zinc chloride DB14548, Zinc sulfate, unspecified form |
PTM databases
GlyConnecti | 716, 1 O-Linked glycan (1 site) |
GlyGeni | P51693, 7 sites, 1 O-linked glycan (2 sites) |
iPTMneti | P51693 |
PhosphoSitePlusi | P51693 |
Genetic variation databases
BioMutai | APLP1 |
DMDMi | 28558769 |
Proteomic databases
MassIVEi | P51693 |
PaxDbi | P51693 |
PeptideAtlasi | P51693 |
PRIDEi | P51693 |
ProteomicsDBi | 56379 [P51693-1] 56380 [P51693-2] |
TopDownProteomicsi | P51693-2 [P51693-2] |
Protocols and materials databases
Antibodypediai | 16162, 247 antibodies |
DNASUi | 333 |
Genome annotation databases
Ensembli | ENST00000221891; ENSP00000221891; ENSG00000105290 [P51693-2] ENST00000652533; ENSP00000498366; ENSG00000105290 [P51693-1] |
GeneIDi | 333 |
KEGGi | hsa:333 |
UCSCi | uc002ocf.4, human [P51693-1] |
Organism-specific databases
CTDi | 333 |
DisGeNETi | 333 |
GeneCardsi | APLP1 |
HGNCi | HGNC:597, APLP1 |
HPAi | ENSG00000105290, Tissue enriched (brain) |
MIMi | 104775, gene |
neXtProti | NX_P51693 |
OpenTargetsi | ENSG00000105290 |
PharmGKBi | PA24884 |
VEuPathDBi | HostDB:ENSG00000105290.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3540, Eukaryota |
GeneTreei | ENSGT00530000063252 |
InParanoidi | P51693 |
OMAi | PELHIAR |
OrthoDBi | 953529at2759 |
PhylomeDBi | P51693 |
TreeFami | TF317274 |
Enzyme and pathway databases
PathwayCommonsi | P51693 |
Miscellaneous databases
BioGRID-ORCSi | 333, 6 hits in 872 CRISPR screens |
ChiTaRSi | APLP1, human |
EvolutionaryTracei | P51693 |
GeneWikii | APLP1 |
GenomeRNAii | 333 |
Pharosi | P51693, Tbio |
PROi | PR:P51693 |
RNActi | P51693, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000105290, Expressed in C1 segment of cervical spinal cord and 168 other tissues |
ExpressionAtlasi | P51693, baseline and differential |
Genevisiblei | P51693, HS |
Family and domain databases
Gene3Di | 1.20.120.770, 1 hit 3.30.1490.140, 1 hit 3.90.570.10, 1 hit |
InterProi | View protein in InterPro IPR036669, Amyloid_Cu-bd_sf IPR008155, Amyloid_glyco IPR011178, Amyloid_glyco_Cu-bd IPR024329, Amyloid_glyco_E2_domain IPR008154, Amyloid_glyco_extra IPR015849, Amyloid_glyco_heparin-bd IPR036454, Amyloid_glyco_heparin-bd_sf IPR019745, Amyloid_glyco_intracell_CS IPR019543, APP_amyloid_C IPR019744, APP_CUBD_CS IPR036176, E2_sf |
PANTHERi | PTHR23103, PTHR23103, 1 hit |
Pfami | View protein in Pfam PF10515, APP_amyloid, 1 hit PF12924, APP_Cu_bd, 1 hit PF12925, APP_E2, 1 hit PF02177, APP_N, 1 hit |
PRINTSi | PR00203, AMYLOIDA4 |
SMARTi | View protein in SMART SM00006, A4_EXTRA, 1 hit |
SUPFAMi | SSF109843, SSF109843, 1 hit SSF56491, SSF56491, 1 hit SSF89811, SSF89811, 1 hit |
PROSITEi | View protein in PROSITE PS00319, APP_CUBD, 1 hit PS51869, APP_E1, 1 hit PS51870, APP_E2, 1 hit PS00320, APP_INTRA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | APLP1_HUMAN | |
Accessioni | P51693Primary (citable) accession number: P51693 Secondary accession number(s): O00113, Q96A92 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | February 22, 2003 | |
Last modified: | February 10, 2021 | |
This is version 189 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM