UniProtKB - P51663 (C11B1_SHEEP)
Cytochrome P450 11B1, mitochondrial
CYP11B1
Functioni
A cytochrome P450 monooxygenase involved in the biosynthesis of adrenal corticoids. Catalyzes the hydroxylation of carbon hydrogen bond at 11-beta position of 11-deoxycortisol and 11-deoxycorticosterone/21-hydroxyprogesterone yielding cortisol or corticosterone, respectively. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).
By similarityCatalytic activityi
- a steroid + 2 H+ + O2 + 2 reduced [adrenodoxin] = an 11β-hydroxysteroid + H2O + 2 oxidized [adrenodoxin]By similarityEC:1.14.15.4By similarityThis reaction proceeds in the forwardBy similarity direction.
- 11-deoxycortisol + 2 H+ + O2 + 2 reduced [adrenodoxin] = cortisol + H2O + 2 oxidized [adrenodoxin]By similarityThis reaction proceeds in the forwardBy similarity direction.
- 21-hydroxyprogesterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = corticosterone + H2O + 2 oxidized [adrenodoxin]By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
: glucocorticoid biosynthesis Pathwayi
This protein is involved in the pathway glucocorticoid biosynthesis, which is part of Steroid biosynthesis.By similarityView all proteins of this organism that are known to be involved in the pathway glucocorticoid biosynthesis and in Steroid biosynthesis.
Pathwayi: Steroid hormone biosynthesis
This protein is involved in Steroid hormone biosynthesis.By similarityView all proteins of this organism that are known to be involved in Steroid hormone biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 450 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- steroid 11-beta-monooxygenase activity Source: UniProtKB-EC
GO - Biological processi
- glucocorticoid biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Steroidogenesis |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00788 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P450 11B1, mitochondrialAlternative name(s): CYPXIB1 Cytochrome P450C11 Steroid 11-beta-hydroxylase, CYP11B1By similarity (EC:1.14.15.4By similarity) |
Gene namesi | Name:CYP11B1 |
Organismi | Ovis aries (Sheep) |
Taxonomic identifieri | 9940 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis |
Proteomesi |
|
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 24 | MitochondrionAdd BLAST | 24 | |
ChainiPRO_0000003606 | 25 – 503 | Cytochrome P450 11B1, mitochondrialAdd BLAST | 479 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0159, Eukaryota |
OMAi | QYTIEAS |
OrthoDBi | 481145at2759 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MALWAKARVW MAGPWLSLHR ARPLGTRASA APKAVLPFEA MPRCPGNKWM
60 70 80 90 100
RVLQIWKEQG SENMHLDMHQ TFQELGPIFR YDVGGRHMVF VMLPEDVERL
110 120 130 140 150
QQAESLHPQR MLLEPWLAYR QARGHKCGVF LLNGPQWRLD RLRLNPDVLS
160 170 180 190 200
LPALQKYTPL VDGVARDFSQ TLKARVLQNA RGSLTLDIAP SVFRYTIEAS
210 220 230 240 250
TLVLYGERLG LLTQQPNPDS LNFIHALEAM FKSTVQLMFV PRRLSRWTSS
260 270 280 290 300
SMWREHFEAW DYIFQYANRA IQRIYQELAL GHPWHYSGIV AELLMRADMT
310 320 330 340 350
LDTIKANTID LTAGSVDTTA FPLLMTLFEL ARNPEVQQAL RQESLVAEAR
360 370 380 390 400
ISENPQRATT ELPLLRAALK ETLRLYPVGI TLERQVSSDL VLQNYHIPAG
410 420 430 440 450
TLVKVLLYSL GRNPAVFARP ESYHPQRWLD RQGSGSRFPH LAFGFGMRQC
460 470 480 490 500
LGRRVAEVEM LLLLHHVLKN FLVETLAQED IKMVYRFILM PSTLPLFTFR
AIQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 99 | R → S in AAB64248 (PubMed:10728821).Curated | 1 | |
Sequence conflicti | 99 | R → S in AAB64249 (PubMed:10728821).Curated | 1 | |
Sequence conflicti | 189 | A → G in AAA62308 (PubMed:7999785).Curated | 1 | |
Sequence conflicti | 439 | P → R in AAA62308 (PubMed:7999785).Curated | 1 | |
Sequence conflicti | 500 | R → G in AAB64248 (PubMed:10728821).Curated | 1 | |
Sequence conflicti | 500 | R → G in AAB64249 (PubMed:10728821).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L34337 mRNA Translation: AAA62308.1 L47569 Genomic DNA Translation: AAA81576.1 U78478 Genomic DNA Translation: AAB64249.1 U78477 mRNA Translation: AAB64248.1 L28716 Genomic DNA Translation: AAA83384.1 |
PIRi | S52085 |
RefSeqi | NP_001068568.1, NM_001075100.2 |
Genome annotation databases
Ensembli | ENSOART00020020700; ENSOARP00020017139; ENSOARG00020013254 |
GeneIDi | 767576 |
KEGGi | oas:767576 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L34337 mRNA Translation: AAA62308.1 L47569 Genomic DNA Translation: AAA81576.1 U78478 Genomic DNA Translation: AAB64249.1 U78477 mRNA Translation: AAB64248.1 L28716 Genomic DNA Translation: AAA83384.1 |
PIRi | S52085 |
RefSeqi | NP_001068568.1, NM_001075100.2 |
3D structure databases
SMRi | P51663 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9940.ENSOARP00000000890 |
Genome annotation databases
Ensembli | ENSOART00020020700; ENSOARP00020017139; ENSOARG00020013254 |
GeneIDi | 767576 |
KEGGi | oas:767576 |
Organism-specific databases
CTDi | 1584 |
Phylogenomic databases
eggNOGi | KOG0159, Eukaryota |
OMAi | QYTIEAS |
OrthoDBi | 481145at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00788 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | C11B1_SHEEP | |
Accessioni | P51663Primary (citable) accession number: P51663 Secondary accession number(s): O19181 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | July 15, 1998 | |
Last modified: | April 7, 2021 | |
This is version 97 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families