UniProtKB - P51647 (AL1A1_RAT)
Protein
Retinal dehydrogenase 1
Gene
Aldh1a1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal (PubMed:7832787). May have a broader specificity and oxidize other aldehydes in vivo (By similarity).By similarity1 Publication
Catalytic activityi
- EC:1.2.1.361 Publication
Activity regulationi
Inhibited by chloral hydrate.1 Publication
Kineticsi
Has more than 2-fold higher catalytic efficiency for 9-cis retinal compared to all-trans retinal and 11-cis retinal.1 Publication
- KM=5.7 µM for 9-cis retinal (at pH 7.5 and 25 degrees Celsius)1 Publication
- KM=9.8 µM for all-trans retinal (at pH 7.5 and 25 degrees Celsius)1 Publication
- KM=10.5 µM for 11-cis retinal (at pH 7.5 and 25 degrees Celsius)1 Publication
: retinol metabolism Pathwayi
This protein is involved in the pathway retinol metabolism, which is part of Cofactor metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway retinol metabolism and in Cofactor metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 170 | Transition state stabilizerBy similarity | 1 | |
Active sitei | 269 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Active sitei | 303 | NucleophilePROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 167 – 170 | NADBy similarity | 4 | |
Nucleotide bindingi | 193 – 196 | NADBy similarity | 4 | |
Nucleotide bindingi | 226 – 227 | NADBy similarity | 2 | |
Nucleotide bindingi | 246 – 247 | NADBy similarity | 2 | |
Nucleotide bindingi | 269 – 271 | NADBy similarity | 3 | |
Nucleotide bindingi | 349 – 353 | NADBy similarity | 5 | |
Nucleotide bindingi | 400 – 402 | NADBy similarity | 3 |
GO - Molecular functioni
- 3-chloroallyl aldehyde dehydrogenase activity Source: RGD
- aldehyde dehydrogenase (NAD+) activity Source: RGD
- benzaldehyde dehydrogenase (NAD+) activity Source: RGD
- identical protein binding Source: RGD
- NAD binding Source: CAFA
- retinal dehydrogenase activity Source: UniProtKB
GO - Biological processi
- 9-cis-retinoic acid biosynthetic process Source: RGD
- 9-cis-retinoic acid metabolic process Source: RGD
- embryonic eye morphogenesis Source: RGD
- estrous cycle Source: RGD
- kidney development Source: RGD
- liver development Source: RGD
- midgut development Source: RGD
- negative regulation of cold-induced thermogenesis Source: YuBioLab
- optic cup morphogenesis involved in camera-type eye development Source: RGD
- oxidation-reduction process Source: UniProtKB
- positive regulation of apoptotic process Source: RGD
- response to drug Source: RGD
- response to estradiol Source: RGD
- response to ethanol Source: RGD
- response to organic cyclic compound Source: RGD
- response to oxidative stress Source: RGD
- response to retinoic acid Source: RGD
- retinoic acid biosynthetic process Source: RGD
- retinoic acid metabolic process Source: RGD
- retinoid metabolic process Source: UniProtKB
- retinol metabolic process Source: RGD
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD |
Enzyme and pathway databases
BRENDAi | 1.2.1.36, 5301 |
Reactomei | R-RNO-5365859, RA biosynthesis pathway R-RNO-70350, Fructose catabolism R-RNO-71384, Ethanol oxidation |
SABIO-RKi | P51647 |
UniPathwayi | UPA00912 |
Chemistry databases
SwissLipidsi | SLP:000000800 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2087, Aldh1a1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity1 Publication | |||
ChainiPRO_0000056419 | 2 – 501 | Retinal dehydrogenase 1Add BLAST | 500 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 91 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 128 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 252 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 337 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 353 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 367 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 410 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 413 | PhosphoserineCombined sources | 1 | |
Modified residuei | 419 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 435 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 495 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
The N-terminus is blocked most probably by acetylation.1 Publication
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P51647 |
PaxDbi | P51647 |
PRIDEi | P51647 |
PTM databases
iPTMneti | P51647 |
PhosphoSitePlusi | P51647 |
Expressioni
Tissue specificityi
Strongly expressed in kidney, lung, testis, intestine, stomach, and trachea, but weakly in the liver.1 Publication
Gene expression databases
Genevisiblei | P51647, RN |
Interactioni
Subunit structurei
Homotetramer.
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000024000 |
Chemistry databases
BindingDBi | P51647 |
Family & Domainsi
Sequence similaritiesi
Belongs to the aldehyde dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | KOG2450, Eukaryota |
HOGENOMi | CLU_005391_0_2_1 |
InParanoidi | P51647 |
OrthoDBi | 153834at2759 |
PhylomeDBi | P51647 |
TreeFami | TF300455 |
Family and domain databases
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016160, Ald_DH_CS_CYS IPR029510, Ald_DH_CS_GLU IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit |
PROSITEi | View protein in PROSITE PS00070, ALDEHYDE_DEHYDR_CYS, 1 hit PS00687, ALDEHYDE_DEHYDR_GLU, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P51647-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSPAQPAVP APLANLKIQH TKIFINNEWH DSVSGKKFPV LNPATEEVIC
60 70 80 90 100
HVEEGDKADV DKAVKAARQA FQIGSPWRTM DASERGRLLN KLADLMERDR
110 120 130 140 150
LLLATIEAIN GGKVFANAYL SDLGGSIKAL KYCAGWADKI HGQTIPSDGD
160 170 180 190 200
IFTFTRREPI GVCGQIIPWN FPLLMFIWKI GPALSCGNTV VVKPAEQTPL
210 220 230 240 250
TALHMASLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDVD KVAFTGSTQV
260 270 280 290 300
GKLIKEAAGK SNLKRVTLEL GGKSPCIVFA DADLDIAVEF AHHGVFYHQG
310 320 330 340 350
QCCVAASRIF VEESVYDEFV RKSVERAKKY VLGNPLTQGI NQGPQIDKEQ
360 370 380 390 400
HDKILDLIES GKKEGAKLEC GGGRWGNKGF FVQPTVFSNV TDEMRIAKEE
410 420 430 440 450
IFGPVQQIMK FKSIDDVIKR ANNTTYGLAA GVFTKDLDRA ITVSSALQAG
460 470 480 490 500
VVWVNCYMIL SAQCPFGGFK MSGNGRELGE HGLYEYTELK TVAMKISQKN
S
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0H2UHP1 | A0A0H2UHP1_RAT | Retinal dehydrogenase 1 | Aldh1a1 | 521 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 100 | R → C in AAA96657 (PubMed:8543180).Curated | 1 | |
Sequence conflicti | 106 | I → M AA sequence (PubMed:7832787).Curated | 1 | |
Sequence conflicti | 170 | N → E in AAA96657 (PubMed:8543180).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L42009 mRNA Translation: AAA96657.1 AF001896 mRNA Translation: AAC53304.1 AF001898 mRNA Translation: AAC53306.1 AF001897 mRNA Translation: AAC53305.1 U79118 mRNA Translation: AAB63423.1 BC061526 mRNA Translation: AAH61526.1 |
PIRi | JC4524 JC5553 |
RefSeqi | NP_071852.2, NM_022407.3 |
Genome annotation databases
GeneIDi | 24188 |
KEGGi | rno:24188 |
UCSCi | RGD:2087, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L42009 mRNA Translation: AAA96657.1 AF001896 mRNA Translation: AAC53304.1 AF001898 mRNA Translation: AAC53306.1 AF001897 mRNA Translation: AAC53305.1 U79118 mRNA Translation: AAB63423.1 BC061526 mRNA Translation: AAH61526.1 |
PIRi | JC4524 JC5553 |
RefSeqi | NP_071852.2, NM_022407.3 |
3D structure databases
SMRi | P51647 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000024000 |
Chemistry databases
BindingDBi | P51647 |
ChEMBLi | CHEMBL2931 |
SwissLipidsi | SLP:000000800 |
PTM databases
iPTMneti | P51647 |
PhosphoSitePlusi | P51647 |
Proteomic databases
jPOSTi | P51647 |
PaxDbi | P51647 |
PRIDEi | P51647 |
Genome annotation databases
GeneIDi | 24188 |
KEGGi | rno:24188 |
UCSCi | RGD:2087, rat |
Organism-specific databases
CTDi | 216 |
RGDi | 2087, Aldh1a1 |
Phylogenomic databases
eggNOGi | KOG2450, Eukaryota |
HOGENOMi | CLU_005391_0_2_1 |
InParanoidi | P51647 |
OrthoDBi | 153834at2759 |
PhylomeDBi | P51647 |
TreeFami | TF300455 |
Enzyme and pathway databases
UniPathwayi | UPA00912 |
BRENDAi | 1.2.1.36, 5301 |
Reactomei | R-RNO-5365859, RA biosynthesis pathway R-RNO-70350, Fructose catabolism R-RNO-71384, Ethanol oxidation |
SABIO-RKi | P51647 |
Miscellaneous databases
PROi | PR:P51647 |
Gene expression databases
Genevisiblei | P51647, RN |
Family and domain databases
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016160, Ald_DH_CS_CYS IPR029510, Ald_DH_CS_GLU IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit |
PROSITEi | View protein in PROSITE PS00070, ALDEHYDE_DEHYDR_CYS, 1 hit PS00687, ALDEHYDE_DEHYDR_GLU, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AL1A1_RAT | |
Accessioni | P51647Primary (citable) accession number: P51647 Secondary accession number(s): O09184 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 154 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families