UniProtKB - P51601 (GCH1_YEAST)
Protein
GTP cyclohydrolase 1
Gene
FOL2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
GTP cyclohydrolase 1 is the first enzyme in the biosynthetic pathway leading to folic acid.
Miscellaneous
Present with 2840 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:3.5.4.16
Activity regulationi
GTP shows a positive allosteric effect, and tetrahydrobiopterin inhibits the enzyme activity.By similarity
: 7,8-dihydroneopterin triphosphate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP.Proteins known to be involved in this subpathway in this organism are:
- GTP cyclohydrolase 1 (FOL2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP, the pathway 7,8-dihydroneopterin triphosphate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 132 | ZincBy similarity | 1 | |
Metal bindingi | 135 | ZincBy similarity | 1 | |
Metal bindingi | 203 | ZincBy similarity | 1 |
GO - Molecular functioni
- GTP binding Source: GO_Central
- GTP cyclohydrolase I activity Source: SGD
- zinc ion binding Source: GO_Central
GO - Biological processi
- 7,8-dihydroneopterin 3'-triphosphate biosynthetic process Source: UniProtKB-UniPathway
- folic acid biosynthetic process Source: UniProtKB-KW
- folic acid-containing compound biosynthetic process Source: SGD
- tetrahydrobiopterin biosynthetic process Source: GO_Central
- tetrahydrofolate biosynthetic process Source: InterPro
Keywordsi
Molecular function | Allosteric enzyme, Hydrolase |
Biological process | Folate biosynthesis |
Ligand | GTP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-SCE-1474151, Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation |
UniPathwayi | UPA00848;UER00151 |
Names & Taxonomyi
Protein namesi | Recommended name: GTP cyclohydrolase 1 (EC:3.5.4.16)Alternative name(s): GTP cyclohydrolase I Short name: GTP-CH-I |
Gene namesi | Name:FOL2 Ordered Locus Names:YGR267C ORF Names:G9349 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003499, FOL2 |
VEuPathDBi | FungiDB:YGR267C |
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119489 | 1 – 243 | GTP cyclohydrolase 1Add BLAST | 243 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 15 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 23 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P51601 |
PaxDbi | P51601 |
PRIDEi | P51601 |
PTM databases
iPTMneti | P51601 |
Interactioni
Subunit structurei
Toroid-shaped homodecamer, composed of two pentamers of five dimers.
By similarityProtein-protein interaction databases
BioGRIDi | 33517, 49 interactors |
DIPi | DIP-1318N |
IntActi | P51601, 15 interactors |
MINTi | P51601 |
STRINGi | 4932.YGR267C |
Miscellaneous databases
RNActi | P51601, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the GTP cyclohydrolase I family.Curated
Phylogenomic databases
eggNOGi | KOG2698, Eukaryota |
GeneTreei | ENSGT00390000013481 |
HOGENOMi | CLU_049768_1_3_1 |
InParanoidi | P51601 |
OMAi | IVVVECE |
Family and domain databases
Gene3Di | 1.10.286.10, 1 hit 3.30.1130.10, 1 hit |
HAMAPi | MF_00223, FolE, 1 hit |
InterProi | View protein in InterPro IPR043133, GTP-CH-I_C/QueF IPR043134, GTP-CH-I_N IPR001474, GTP_CycHdrlase_I IPR018234, GTP_CycHdrlase_I_CS IPR020602, GTP_CycHdrlase_I_dom |
PANTHERi | PTHR11109, PTHR11109, 1 hit |
Pfami | View protein in Pfam PF01227, GTP_cyclohydroI, 1 hit |
TIGRFAMsi | TIGR00063, folE, 1 hit |
PROSITEi | View protein in PROSITE PS00859, GTP_CYCLOHYDROL_1_1, 1 hit PS00860, GTP_CYCLOHYDROL_1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P51601-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHNIQLVQEI ERHETPLNIR PTSPYTLNPP VERDGFSWPS VGTRQRAEET
60 70 80 90 100
EEEEKERIQR ISGAIKTILT ELGEDVNREG LLDTPQRYAK AMLYFTKGYQ
110 120 130 140 150
TNIMDDVIKN AVFEEDHDEM VIVRDIEIYS LCEHHLVPFF GKVHIGYIPN
160 170 180 190 200
KKVIGLSKLA RLAEMYARRL QVQERLTKQI AMALSDILKP LGVAVVMEAS
210 220 230 240
HMCMVSRGIQ KTGSSTVTSC MLGGFRAHKT REEFLTLLGR RSI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 159 – 160 | LA → QG in CAA89826 (PubMed:7542887).Curated | 2 | |
Sequence conflicti | 206 | S → M in CAA89826 (PubMed:7542887).Curated | 1 | |
Sequence conflicti | 217 | V → A in CAA87397 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z47201 Genomic DNA Translation: CAA87397.1 X94314 Genomic DNA Translation: CAA63975.1 Y07893 Genomic DNA Translation: CAA69198.1 Z73052 Genomic DNA Translation: CAA97297.1 AY692993 Genomic DNA Translation: AAT93012.1 Z49756 mRNA Translation: CAA89826.1 BK006941 Genomic DNA Translation: DAA08356.1 |
PIRi | JC4585 |
RefSeqi | NP_011783.1, NM_001181396.1 |
Genome annotation databases
EnsemblFungii | YGR267C_mRNA; YGR267C; YGR267C |
GeneIDi | 853183 |
KEGGi | sce:YGR267C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z47201 Genomic DNA Translation: CAA87397.1 X94314 Genomic DNA Translation: CAA63975.1 Y07893 Genomic DNA Translation: CAA69198.1 Z73052 Genomic DNA Translation: CAA97297.1 AY692993 Genomic DNA Translation: AAT93012.1 Z49756 mRNA Translation: CAA89826.1 BK006941 Genomic DNA Translation: DAA08356.1 |
PIRi | JC4585 |
RefSeqi | NP_011783.1, NM_001181396.1 |
3D structure databases
SMRi | P51601 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33517, 49 interactors |
DIPi | DIP-1318N |
IntActi | P51601, 15 interactors |
MINTi | P51601 |
STRINGi | 4932.YGR267C |
PTM databases
iPTMneti | P51601 |
Proteomic databases
MaxQBi | P51601 |
PaxDbi | P51601 |
PRIDEi | P51601 |
Genome annotation databases
EnsemblFungii | YGR267C_mRNA; YGR267C; YGR267C |
GeneIDi | 853183 |
KEGGi | sce:YGR267C |
Organism-specific databases
SGDi | S000003499, FOL2 |
VEuPathDBi | FungiDB:YGR267C |
Phylogenomic databases
eggNOGi | KOG2698, Eukaryota |
GeneTreei | ENSGT00390000013481 |
HOGENOMi | CLU_049768_1_3_1 |
InParanoidi | P51601 |
OMAi | IVVVECE |
Enzyme and pathway databases
UniPathwayi | UPA00848;UER00151 |
Reactomei | R-SCE-1474151, Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation |
Miscellaneous databases
PROi | PR:P51601 |
RNActi | P51601, protein |
Family and domain databases
Gene3Di | 1.10.286.10, 1 hit 3.30.1130.10, 1 hit |
HAMAPi | MF_00223, FolE, 1 hit |
InterProi | View protein in InterPro IPR043133, GTP-CH-I_C/QueF IPR043134, GTP-CH-I_N IPR001474, GTP_CycHdrlase_I IPR018234, GTP_CycHdrlase_I_CS IPR020602, GTP_CycHdrlase_I_dom |
PANTHERi | PTHR11109, PTHR11109, 1 hit |
Pfami | View protein in Pfam PF01227, GTP_cyclohydroI, 1 hit |
TIGRFAMsi | TIGR00063, folE, 1 hit |
PROSITEi | View protein in PROSITE PS00859, GTP_CYCLOHYDROL_1_1, 1 hit PS00860, GTP_CYCLOHYDROL_1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GCH1_YEAST | |
Accessioni | P51601Primary (citable) accession number: P51601 Secondary accession number(s): D6VV45 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 174 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families