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UniProtKB - P51590 (CP2J3_RAT)
Protein
Cytochrome P450 2J3
Gene
Cyp2j3
Organism
Rattus norvegicus (Rat)
Status
Functioni
This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation mainly to 14,15-, 11,12-, and 8,9-epoxyeicosatrienoic acids (EET). It also acts as an omega-1-hydroxylase by metabolizing arachidonic acid to 19-hydroxyeicosatetraenoic acid (19-OH-AA).
Catalytic activityi
- an organic molecule + O2 + reduced [NADPH—hemoprotein reductase] = an alcohol + H+ + H2O + oxidized [NADPH—hemoprotein reductase]EC:1.14.14.1
Cofactori
hemeBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 448 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- arachidonic acid 11,12-epoxygenase activity Source: Ensembl
- arachidonic acid 14,15-epoxygenase activity Source: Ensembl
- aromatase activity Source: UniProtKB-EC
- heme binding Source: GO_Central
- iron ion binding Source: InterPro
- isomerase activity Source: Ensembl
- linoleic acid epoxygenase activity Source: Ensembl
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
- steroid hydroxylase activity Source: GO_Central
GO - Biological processi
- epoxygenase P450 pathway Source: Ensembl
- linoleic acid metabolic process Source: Ensembl
- organic acid metabolic process Source: GO_Central
- xenobiotic metabolic process Source: GO_Central
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
BRENDAi | 1.14.14.24, 5301 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Cyp2j3 Synonyms:Cyp2j9 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 631442, Cyp2j3 |
Subcellular locationi
Endoplasmic reticulum
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: GO_Central
- intracellular membrane-bounded organelle Source: GO_Central
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051770 | 1 – 502 | Cytochrome P450 2J3Add BLAST | 502 |
Proteomic databases
PRIDEi | P51590 |
PTM databases
PhosphoSitePlusi | P51590 |
Expressioni
Tissue specificityi
Abundantly expressed in heart and liver.
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Phylogenomic databases
InParanoidi | P51590 |
OrthoDBi | 702827at2759 |
PhylomeDBi | P51590 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR008071, Cyt_P450_E_grp-I_CYP2J-like IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR01688, EP450ICYP2J PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
P51590-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLVTAGSLLG AIWTVLHLRI LLLAAVTFLF LADFLKHRRP KNYPPGPWRL
60 70 80 90 100
PLVGCLFHLD PKQPHLSLQQ FVKKYGNVLS LDFANIPSVV VTGMPLIKEI
110 120 130 140 150
FTQMEHNFLN RPVTLLRKHL FNKNGLIFSS GQTWKEQRRF ALMTLRNFGL
160 170 180 190 200
GKKSLEQRIQ EEAYHLVEAI KDEGGLPFDP HFNINKAVSN IICSVTFGER
210 220 230 240 250
FEYHDSQFQE MLRLLDEAMC LESSMMCQLY NIFPRILQYL PGSHQTLFSN
260 270 280 290 300
WRKLKLFISD IIKNHRRDWD PDEPRDFIDA FLKEMAKYPD KTTTSFNEEN
310 320 330 340 350
LICSTLDLFF AGTETTSTTL RWALLCMALY PEVQEKMQAE IDRVIGQGRQ
360 370 380 390 400
PNLADRDSMP YTNAVIHEVQ RIGNIIPFNV PREVAVDTYL AGFNLPKGTM
410 420 430 440 450
ILTNLTALHR DPKEWATPDT FNPEHFLENG QFKKRESFLP FSMGKRACLG
460 470 480 490 500
EQLARSELFI FITSLIQKFT FKPPVNEKLS LQFRMSVTIS PVSHRLCAIP
RL
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5BKA2 | Q5BKA2_RAT | Cytochrome P450 2J3 | Cyp2j4 Cyp2j3, rCG_53393 | 501 | Annotation score: | ||
F1M032 | F1M032_RAT | Cytochrome P450, family 2, subfamil... | Cyp2j10 LOC100912642 | 502 | Annotation score: | ||
F1LVB0 | F1LVB0_RAT | Cytochrome P450 2J3 | Cyp2j10 Cyp2j3, LOC100912642 | 468 | Annotation score: | ||
A0A0G2K921 | A0A0G2K921_RAT | Cytochrome P450 2J3 | Cyp2j10 Cyp2j3 | 432 | Annotation score: | ||
E9PSJ0 | E9PSJ0_RAT | Cytochrome P450, family 2, subfamil... | Cyp2j10 | 502 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U39943 mRNA Translation: AAB48545.1 BC089766 mRNA Translation: AAH89766.1 |
RefSeqi | NP_786942.1, NM_175766.3 |
Genome annotation databases
Ensembli | ENSRNOT00000058163; ENSRNOP00000054968; ENSRNOG00000031004 |
GeneIDi | 313375 |
KEGGi | rno:313375 |
UCSCi | RGD:631442, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U39943 mRNA Translation: AAB48545.1 BC089766 mRNA Translation: AAH89766.1 |
RefSeqi | NP_786942.1, NM_175766.3 |
3D structure databases
AlphaFoldDBi | P51590 |
SMRi | P51590 |
ModBasei | Search... |
PTM databases
PhosphoSitePlusi | P51590 |
Proteomic databases
PRIDEi | P51590 |
Genome annotation databases
Ensembli | ENSRNOT00000058163; ENSRNOP00000054968; ENSRNOG00000031004 |
GeneIDi | 313375 |
KEGGi | rno:313375 |
UCSCi | RGD:631442, rat |
Organism-specific databases
CTDi | 313375 |
RGDi | 631442, Cyp2j3 |
Phylogenomic databases
InParanoidi | P51590 |
OrthoDBi | 702827at2759 |
PhylomeDBi | P51590 |
Enzyme and pathway databases
BRENDAi | 1.14.14.24, 5301 |
Miscellaneous databases
PROi | PR:P51590 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR008071, Cyt_P450_E_grp-I_CYP2J-like IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR01688, EP450ICYP2J PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CP2J3_RAT | |
Accessioni | P51590Primary (citable) accession number: P51590 Secondary accession number(s): Q5EBD7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families