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Protein

Cytochrome P450 2J2

Gene

CYP2J2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi448Iron (heme axial ligand)By similarity1

GO - Molecular functioni

  • arachidonic acid 11,12-epoxygenase activity Source: BHF-UCL
  • arachidonic acid 14,15-epoxygenase activity Source: BHF-UCL
  • arachidonic acid epoxygenase activity Source: BHF-UCL
  • aromatase activity Source: UniProtKB-EC
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • linoleic acid epoxygenase activity Source: BHF-UCL
  • monooxygenase activity Source: Reactome

GO - Biological processi

  • epoxygenase P450 pathway Source: BHF-UCL
  • icosanoid metabolic process Source: ProtInc
  • linoleic acid metabolic process Source: BHF-UCL
  • regulation of heart contraction Source: ProtInc
  • xenobiotic metabolic process Source: BHF-UCL

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS05902-MONOMER
BRENDAi1.14.13.159 2681
ReactomeiR-HSA-211935 Fatty acids
R-HSA-211981 Xenobiotics
R-HSA-2142670 Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET)

Chemistry databases

SwissLipidsiSLP:000001594

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2J2 (EC:1.14.14.1)
Alternative name(s):
Arachidonic acid epoxygenase
CYPIIJ2
Gene namesi
Name:CYP2J2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000134716.9
HGNCiHGNC:2634 CYP2J2
MIMi601258 gene
neXtProtiNX_P51589

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

DisGeNETi1573
OpenTargetsiENSG00000134716
PharmGKBiPA27112

Chemistry databases

ChEMBLiCHEMBL3491
DrugBankiDB06605 Apixaban
DB00637 Astemizole
DB00169 Cholecalciferol
DB09148 Florbetaben (18F)
DB08918 Levomilnacipran
DB04725 Licofelone
DB00179 Masoprocol
DB08931 Riociguat
DB06228 Rivaroxaban
DB00342 Terfenadine
GuidetoPHARMACOLOGYi1332

Polymorphism and mutation databases

BioMutaiCYP2J2
DMDMi21264413

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000517691 – 502Cytochrome P450 2J2Add BLAST502

Proteomic databases

MaxQBiP51589
PaxDbiP51589
PeptideAtlasiP51589
PRIDEiP51589
ProteomicsDBi56341

PTM databases

iPTMnetiP51589
PhosphoSitePlusiP51589

Expressioni

Tissue specificityi

Highly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney.

Gene expression databases

BgeeiENSG00000134716
CleanExiHS_CYP2J2
GenevisibleiP51589 HS

Interactioni

Protein-protein interaction databases

BioGridi107946, 27 interactors
IntActiP51589, 2 interactors
STRINGi9606.ENSP00000360247

Chemistry databases

BindingDBiP51589

Structurei

3D structure databases

ProteinModelPortaliP51589
SMRiP51589
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00900000140799
HOGENOMiHOG000036991
HOVERGENiHBG015789
InParanoidiP51589
KOiK07418
OMAiRTIFSCN
OrthoDBiEOG091G0BT8
PhylomeDBiP51589
TreeFamiTF352043

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR008071 Cyt_P450_E_grp-I_CYP2J-like
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR01688 EP450ICYP2J
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

P51589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAAMGSLAA ALWAVVHPRT LLLGTVAFLL AADFLKRRRP KNYPPGPWRL
60 70 80 90 100
PFLGNFFLVD FEQSHLEVQL FVKKYGNLFS LELGDISAVL ITGLPLIKEA
110 120 130 140 150
LIHMDQNFGN RPVTPMREHI FKKNGLIMSS GQAWKEQRRF TLTALRNFGL
160 170 180 190 200
GKKSLEERIQ EEAQHLTEAI KEENGQPFDP HFKINNAVSN IICSITFGER
210 220 230 240 250
FEYQDSWFQQ LLKLLDEVTY LEASKTCQLY NVFPWIMKFL PGPHQTLFSN
260 270 280 290 300
WKKLKLFVSH MIDKHRKDWN PAETRDFIDA YLKEMSKHTG NPTSSFHEEN
310 320 330 340 350
LICSTLDLFF AGTETTSTTL RWALLYMALY PEIQEKVQAE IDRVIGQGQQ
360 370 380 390 400
PSTAARESMP YTNAVIHEVQ RMGNIIPLNV PREVTVDTTL AGYHLPKGTM
410 420 430 440 450
ILTNLTALHR DPTEWATPDT FNPDHFLENG QFKKREAFMP FSIGKRACLG
460 470 480 490 500
EQLARTELFI FFTSLMQKFT FRPPNNEKLS LKFRMGITIS PVSHRLCAVP

QV
Length:502
Mass (Da):57,611
Last modified:May 27, 2002 - v2
Checksum:iB5F006581E249EC0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti264K → E in BAG37780 (PubMed:14702039).Curated1
Sequence conflicti318T → A in BAG37780 (PubMed:14702039).Curated1
Sequence conflicti339A → V in BAB85489 (PubMed:12386130).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02915949R → S1 PublicationCorresponds to variant dbSNP:rs11572190Ensembl.1
Natural variantiVAR_029160113V → M. Corresponds to variant dbSNP:rs11572242Ensembl.1
Natural variantiVAR_022084124N → S. Corresponds to variant dbSNP:rs2228113Ensembl.1
Natural variantiVAR_014317143T → A in allele CYP2J2*2; significantly reduced metabolism of both arachidonic acid and linoleic acid. 1 PublicationCorresponds to variant dbSNP:rs55753213Ensembl.1
Natural variantiVAR_014318158R → C in allele CYP2J2*3; significantly reduced metabolism of both arachidonic acid and linoleic acid. 1 PublicationCorresponds to variant dbSNP:rs56307989Ensembl.1
Natural variantiVAR_014319192I → N in allele CYP2J2*4; significantly reduced metabolism of arachidonic acid only. 1 PublicationCorresponds to variant dbSNP:rs66515830Ensembl.1
Natural variantiVAR_014320342D → N in allele CYP2J2*5; no change in activity. 1 PublicationCorresponds to variant dbSNP:rs56053398Ensembl.1
Natural variantiVAR_014321404N → Y in allele CYP2J2*6; significantly reduced metabolism of both arachidonic acid and linoleic acid. 1 PublicationCorresponds to variant dbSNP:rs72547598Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37143 mRNA Translation: AAC50370.2
AB080265 mRNA Translation: BAB85489.1
AF272142 Genomic DNA Translation: AAM44456.1
AY426985 Genomic DNA Translation: AAQ93356.1
AK315387 mRNA Translation: BAG37780.1
BC032594 mRNA Translation: AAH32594.1
CCDSiCCDS613.1
RefSeqiNP_000766.2, NM_000775.3
UniGeneiHs.152096

Genome annotation databases

EnsembliENST00000371204; ENSP00000360247; ENSG00000134716
GeneIDi1573
KEGGihsa:1573
UCSCiuc001czq.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCP2J2_HUMAN
AccessioniPrimary (citable) accession number: P51589
Secondary accession number(s): B2RD33, Q8TF13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 27, 2002
Last modified: June 20, 2018
This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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