UniProtKB - P51583 (PUR6_RAT)
Protein
Multifunctional protein ADE2
Gene
Paics
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalytic activityi
- 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate = (2S)-2-[5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H+ + phosphateEC:6.3.2.6
- 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + H+ = 5-amino-1-(5-phospho-β-D-ribosyl)imidazole + CO2EC:4.1.1.21
: IMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Multifunctional protein ADE2 (Paics), AIR carboxylase, AIR carboxylase (LOC100910308)
- Adenylosuccinate lyase (Adsl)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Pathwayi: IMP biosynthesis via de novo pathway
This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route).Proteins known to be involved in this subpathway in this organism are:
- Multifunctional protein ADE2 (Paics), AIR carboxylase, AIR carboxylase (LOC100910308)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 101 | For SAICAR synthetase activityBy similarity | 1 | |
Active sitei | 107 | For SAICAR synthetase activityBy similarity | 1 | |
Active sitei | 215 | For SAICAR synthetase activityBy similarity | 1 | |
Active sitei | 303 | For AIR carboxylase activityBy similarity | 1 | |
Active sitei | 332 | For AIR carboxylase activityBy similarity | 1 | |
Sitei | 334 | Essential for AIR carboxylase activityBy similarity | 1 |
GO - Molecular functioni
- 5-amino-4-imidazole carboxylate lyase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- identical protein binding Source: RGD
- phosphoribosylaminoimidazole carboxylase activity Source: UniProtKB-EC
- phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Source: UniProtKB-EC
GO - Biological processi
- 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
- G1/S transition of mitotic cell cycle Source: RGD
Keywordsi
Molecular function | Decarboxylase, Ligase, Lyase, Multifunctional enzyme |
Biological process | Purine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-73817, Purine ribonucleoside monophosphate biosynthesis |
UniPathwayi | UPA00074;UER00130 UPA00074;UER00131 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Paics Synonyms:Ade2, Airc |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620066, Paics |
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000075033 | 2 – 425 | Multifunctional protein ADE2Add BLAST | 424 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 22 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 27 | PhosphoserineCombined sources | 1 | |
Modified residuei | 36 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 107 | PhosphoserineBy similarity | 1 | |
Modified residuei | 238 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 247 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 274 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P51583 |
PaxDbi | P51583 |
PRIDEi | P51583 |
PTM databases
iPTMneti | P51583 |
PhosphoSitePlusi | P51583 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000002101, Expressed in female gonad and 20 other tissues |
Genevisiblei | P51583, RN |
Interactioni
Subunit structurei
Homooctamer.
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
IntActi | P51583, 2 interactors |
STRINGi | 10116.ENSRNOP00000061495 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 260 | SAICAR synthetaseAdd BLAST | 259 | |
Regioni | 261 – 425 | AIR carboxylaseAdd BLAST | 165 | |
Regioni | 261 – 266 | LinkerBy similarity | 6 | |
Regioni | 267 – 425 | AIR carboxylaseBy similarityAdd BLAST | 159 |
Sequence similaritiesi
In the N-terminal section; belongs to the SAICAR synthetase family.Curated
Phylogenomic databases
eggNOGi | KOG2835, Eukaryota |
GeneTreei | ENSGT00390000010172 |
HOGENOMi | CLU_061495_1_0_1 |
InParanoidi | P51583 |
OMAi | WSDEQII |
OrthoDBi | 566769at2759 |
PhylomeDBi | P51583 |
TreeFami | TF106384 |
Family and domain databases
Gene3Di | 3.40.50.7700, 1 hit |
HAMAPi | MF_02045, PurE_classII, 1 hit MF_00137, SAICAR_synth, 1 hit |
InterProi | View protein in InterPro IPR033626, PurE_classII IPR000031, PurE_dom IPR035893, PurE_sf IPR028923, SAICAR_synt/ADE2_N IPR018236, SAICAR_synthetase_CS |
Pfami | View protein in Pfam PF00731, AIRC, 1 hit PF01259, SAICAR_synt, 1 hit |
SMARTi | View protein in SMART SM01001, AIRC, 1 hit |
SUPFAMi | SSF52255, SSF52255, 1 hit |
TIGRFAMsi | TIGR01162, purE, 1 hit |
PROSITEi | View protein in PROSITE PS01057, SAICAR_SYNTHETASE_1, 1 hit PS01058, SAICAR_SYNTHETASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P51583-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATAEVLNIG RKLYEGKTKE VYELLDSPGR VLLQSKDQIT AGNAARKNHL
60 70 80 90 100
EGKAAISNKI TSCIFQLLQE AGIKTAFTKK CGETAFIAPQ CEMIPIEWVC
110 120 130 140 150
RRIATGSFLK RNPGVKEGYR FYPPKVEMFF KDDANNDPQW SEEQLIAAKF
160 170 180 190 200
CFAGLVIGQT EVDIMSHATQ AIFEILEKSW LPQNCTLVDM KIEFGVDVTT
210 220 230 240 250
KEIVLADVID NDSWRLWPSG DRSQQKDKQS YRDLKEVTPE GLQMVKKNFE
260 270 280 290 300
WVADRVELLL KSNSQCRVVV LMGSTSDLGH CEKIKKACGN FGIPCELRVT
310 320 330 340 350
SAHKGPDETL RIKAEYEGDG IPTVFVAVAG RSNGLGPVMS GNTAYPVISC
360 370 380 390 400
PPITADWGAQ DVWSSLRLPS GIGCSTILSP EGSAQFAAQI FGLNNHLVWA
410 420
KLRASKLNTW ISLKQADKKI RECNL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D37979 mRNA Translation: BAA07197.1 BC072508 mRNA Translation: AAH72508.1 BC085711 mRNA Translation: AAH85711.1 |
PIRi | S55684 |
RefSeqi | NP_543186.1, NM_080910.3 XP_006250914.1, XM_006250852.3 XP_006250915.1, XM_006250853.3 XP_006250916.1, XM_006250854.2 |
Genome annotation databases
Ensembli | ENSRNOT00000063942; ENSRNOP00000061495; ENSRNOG00000002101 |
GeneIDi | 140946 |
KEGGi | rno:140946 |
UCSCi | RGD:620066, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D37979 mRNA Translation: BAA07197.1 BC072508 mRNA Translation: AAH72508.1 BC085711 mRNA Translation: AAH85711.1 |
PIRi | S55684 |
RefSeqi | NP_543186.1, NM_080910.3 XP_006250914.1, XM_006250852.3 XP_006250915.1, XM_006250853.3 XP_006250916.1, XM_006250854.2 |
3D structure databases
SMRi | P51583 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P51583, 2 interactors |
STRINGi | 10116.ENSRNOP00000061495 |
PTM databases
iPTMneti | P51583 |
PhosphoSitePlusi | P51583 |
Proteomic databases
jPOSTi | P51583 |
PaxDbi | P51583 |
PRIDEi | P51583 |
Genome annotation databases
Ensembli | ENSRNOT00000063942; ENSRNOP00000061495; ENSRNOG00000002101 |
GeneIDi | 140946 |
KEGGi | rno:140946 |
UCSCi | RGD:620066, rat |
Organism-specific databases
CTDi | 10606 |
RGDi | 620066, Paics |
Phylogenomic databases
eggNOGi | KOG2835, Eukaryota |
GeneTreei | ENSGT00390000010172 |
HOGENOMi | CLU_061495_1_0_1 |
InParanoidi | P51583 |
OMAi | WSDEQII |
OrthoDBi | 566769at2759 |
PhylomeDBi | P51583 |
TreeFami | TF106384 |
Enzyme and pathway databases
UniPathwayi | UPA00074;UER00130 UPA00074;UER00131 |
Reactomei | R-RNO-73817, Purine ribonucleoside monophosphate biosynthesis |
Miscellaneous databases
PROi | PR:P51583 |
Gene expression databases
Bgeei | ENSRNOG00000002101, Expressed in female gonad and 20 other tissues |
Genevisiblei | P51583, RN |
Family and domain databases
Gene3Di | 3.40.50.7700, 1 hit |
HAMAPi | MF_02045, PurE_classII, 1 hit MF_00137, SAICAR_synth, 1 hit |
InterProi | View protein in InterPro IPR033626, PurE_classII IPR000031, PurE_dom IPR035893, PurE_sf IPR028923, SAICAR_synt/ADE2_N IPR018236, SAICAR_synthetase_CS |
Pfami | View protein in Pfam PF00731, AIRC, 1 hit PF01259, SAICAR_synt, 1 hit |
SMARTi | View protein in SMART SM01001, AIRC, 1 hit |
SUPFAMi | SSF52255, SSF52255, 1 hit |
TIGRFAMsi | TIGR01162, purE, 1 hit |
PROSITEi | View protein in PROSITE PS01057, SAICAR_SYNTHETASE_1, 1 hit PS01058, SAICAR_SYNTHETASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PUR6_RAT | |
Accessioni | P51583Primary (citable) accession number: P51583 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families