UniProtKB - P51577 (P2RX4_RAT)
Protein
P2X purinoceptor 4
Gene
P2rx4
Organism
Rattus norvegicus (Rat)
Status
Functioni
Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.1 Publication
GO - Molecular functioni
- ATP binding Source: RGD
- cadherin binding Source: RGD
- copper ion binding Source: BHF-UCL
- extracellularly ATP-gated cation channel activity Source: UniProtKB
- identical protein binding Source: IntAct
- ligand-gated calcium channel activity Source: ARUK-UCL
- purinergic nucleotide receptor activity Source: BHF-UCL
- signaling receptor binding Source: BHF-UCL
- zinc ion binding Source: BHF-UCL
GO - Biological processi
- apoptotic signaling pathway Source: RGD
- behavioral response to pain Source: ARUK-UCL
- calcium ion transmembrane transport Source: UniProtKB
- calcium ion transport Source: RGD
- cellular response to ATP Source: UniProtKB
- cellular response to zinc ion Source: UniProtKB
- excitatory postsynaptic potential Source: RGD
- inorganic cation transmembrane transport Source: RGD
- ion transmembrane transport Source: RGD
- membrane depolarization Source: RGD
- negative regulation of cardiac muscle hypertrophy Source: RGD
- neuronal action potential Source: RGD
- nitric oxide biosynthetic process Source: RGD
- positive regulation of blood vessel endothelial cell migration Source: RGD
- positive regulation of calcium ion transport into cytosol Source: RGD
- positive regulation of calcium-mediated signaling Source: RGD
- positive regulation of endothelial cell chemotaxis Source: RGD
- positive regulation of microglial cell migration Source: ARUK-UCL
- positive regulation of protein kinase B signaling Source: ARUK-UCL
- purinergic nucleotide receptor signaling pathway Source: RGD
- regulation of blood pressure Source: RGD
- regulation of cardiac muscle contraction Source: RGD
- regulation of chemotaxis Source: ARUK-UCL
- regulation of sodium ion transport Source: BHF-UCL
- relaxation of cardiac muscle Source: RGD
- response to ATP Source: RGD
- response to axon injury Source: ARUK-UCL
- response to fluid shear stress Source: RGD
- response to ischemia Source: ARUK-UCL
- sensory perception of pain Source: ARUK-UCL
- sensory perception of touch Source: ARUK-UCL
- signal transduction Source: RGD
- vasodilation Source: RGD
Keywordsi
Molecular function | Ion channel, Ligand-gated ion channel, Receptor |
Biological process | Ion transport, Transport |
Enzyme and pathway databases
Reactomei | R-RNO-139853, Elevation of cytosolic Ca2+ levels R-RNO-418346, Platelet homeostasis |
Names & Taxonomyi
Protein namesi | Recommended name: P2X purinoceptor 4Short name: P2X4 Alternative name(s): ATP receptor Purinergic receptor |
Gene namesi | Name:P2rx4 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 62073, P2rx4 |
Subcellular locationi
Other locations
- Membrane By similarity; Multi-pass membrane protein Sequence analysis
Nucleus
- integral component of nuclear inner membrane Source: GO_Central
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: BHF-UCL
Other locations
- apical part of cell Source: RGD
- axon Source: RGD
- cell body Source: ARUK-UCL
- cell junction Source: RGD
- dendrite Source: RGD
- dendritic spine Source: RGD
- membrane Source: RGD
- neuronal cell body Source: RGD
- perinuclear region of cytoplasm Source: RGD
- postsynaptic density Source: RGD
- synapse Source: RGD
- terminal bouton Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 33 | CytoplasmicSequence analysisAdd BLAST | 33 | |
Transmembranei | 34 – 54 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 55 – 338 | ExtracellularSequence analysisAdd BLAST | 284 | |
Transmembranei | 339 – 359 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 360 – 388 | CytoplasmicSequence analysisAdd BLAST | 29 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 249 | E → K: Restores antagonism by PPADS. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL2818 |
DrugCentrali | P51577 |
GuidetoPHARMACOLOGYi | 481 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000161554 | 1 – 388 | P2X purinoceptor 4Add BLAST | 388 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 75 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 110 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 116 ↔ 165 | By similarity | ||
Disulfide bondi | 126 ↔ 149 | By similarity | ||
Disulfide bondi | 132 ↔ 159 | By similarity | ||
Glycosylationi | 153 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 184 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 199 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 208 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 217 ↔ 227 | By similarity | ||
Disulfide bondi | 261 ↔ 270 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P51577 |
PRIDEi | P51577 |
PTM databases
GlyGeni | P51577, 6 sites |
PhosphoSitePlusi | P51577 |
Expressioni
Tissue specificityi
Widespread distribution in the brain. Also expressed in epithelial cells.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000001300, Expressed in pancreas and 21 other tissues |
Genevisiblei | P51577, RN |
Interactioni
Subunit structurei
Functional P2XRs are organized as homomeric and heteromeric trimers.
By similarityBinary interactionsi
Hide detailsP51577
With | #Exp. | IntAct |
---|---|---|
P2rx2 [P49653] | 4 | EBI-9511617,EBI-9511543 |
itself | 2 | EBI-9511617,EBI-9511617 |
GO - Molecular functioni
- cadherin binding Source: RGD
- identical protein binding Source: IntAct
- signaling receptor binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 248281, 2 interactors |
DIPi | DIP-48411N |
IntActi | P51577, 2 interactors |
MINTi | P51577 |
STRINGi | 10116.ENSRNOP00000001752 |
Chemistry databases
BindingDBi | P51577 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P51577 |
SMRi | P51577 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P51577 |
Family & Domainsi
Sequence similaritiesi
Belongs to the P2X receptor family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QSUI, Eukaryota |
GeneTreei | ENSGT01010000222290 |
HOGENOMi | CLU_034469_2_0_1 |
InParanoidi | P51577 |
OMAi | RAASHCV |
OrthoDBi | 1128763at2759 |
PhylomeDBi | P51577 |
TreeFami | TF328633 |
Family and domain databases
Gene3Di | 2.60.490.10, 1 hit |
IDEALi | IID50123 |
InterProi | View protein in InterPro IPR003047, P2X4_purnocptor IPR027309, P2X_extracellular_dom_sf IPR001429, P2X_purnocptor |
PIRSFi | PIRSF005713, P2X_purinoceptor, 1 hit |
PRINTSi | PR01311, P2X4RECEPTOR PR01307, P2XRECEPTOR |
TIGRFAMsi | TIGR00863, P2X, 1 hit |
PROSITEi | View protein in PROSITE PS01212, P2X_RECEPTOR, 1 hit |
i Sequence
Sequence statusi: Complete.
P51577-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGCCSVLGS FLFEYDTPRI VLIRSRKVGL MNRAVQLLIL AYVIGWVFVW
60 70 80 90 100
EKGYQETDSV VSSVTTKAKG VAVTNTSQLG FRIWDVADYV IPAQEENSLF
110 120 130 140 150
IMTNMIVTVN QTQSTCPEIP DKTSICNSDA DCTPGSVDTH SSGVATGRCV
160 170 180 190 200
PFNESVKTCE VAAWCPVEND VGVPTPAFLK AAENFTLLVK NNIWYPKFNF
210 220 230 240 250
SKRNILPNIT TSYLKSCIYN AQTDPFCPIF RLGTIVEDAG HSFQEMAVEG
260 270 280 290 300
GIMGIQIKWD CNLDRAASLC LPRYSFRRLD TRDLEHNVSP GYNFRFAKYY
310 320 330 340 350
RDLAGKEQRT LTKAYGIRFD IIVFGKAGKF DIIPTMINVG SGLALLGVAT
360 370 380
VLCDVIVLYC MKKKYYYRDK KYKYVEDYEQ GLSGEMNQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 136 – 137 | SV → LR in AAC52380 (PubMed:8551329).Curated | 2 | |
Sequence conflicti | 237 | E → G in CAA61037 (PubMed:8598206).Curated | 1 | |
Sequence conflicti | 305 | G → A in CAA62607 (PubMed:7498461).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U32497 mRNA Translation: AAC52380.1 X91200 mRNA Translation: CAA62607.1 U47031 mRNA Translation: AAA99777.1 X87763 mRNA Translation: CAA61037.1 X93565 mRNA Translation: CAA63778.1 BC078792 mRNA Translation: AAH78792.1 |
PIRi | JC6137 |
RefSeqi | NP_113782.1, NM_031594.1 |
Genome annotation databases
Ensembli | ENSRNOT00000001752; ENSRNOP00000001752; ENSRNOG00000001300 |
GeneIDi | 29659 |
KEGGi | rno:29659 |
UCSCi | RGD:62073, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U32497 mRNA Translation: AAC52380.1 X91200 mRNA Translation: CAA62607.1 U47031 mRNA Translation: AAA99777.1 X87763 mRNA Translation: CAA61037.1 X93565 mRNA Translation: CAA63778.1 BC078792 mRNA Translation: AAH78792.1 |
PIRi | JC6137 |
RefSeqi | NP_113782.1, NM_031594.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2BP5 | X-ray | 2.80 | P | 375-384 | [»] | |
2RUP | NMR | - | A | 111-167 | [»] | |
BMRBi | P51577 | |||||
SMRi | P51577 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 248281, 2 interactors |
DIPi | DIP-48411N |
IntActi | P51577, 2 interactors |
MINTi | P51577 |
STRINGi | 10116.ENSRNOP00000001752 |
Chemistry databases
BindingDBi | P51577 |
ChEMBLi | CHEMBL2818 |
DrugCentrali | P51577 |
GuidetoPHARMACOLOGYi | 481 |
PTM databases
GlyGeni | P51577, 6 sites |
PhosphoSitePlusi | P51577 |
Proteomic databases
PaxDbi | P51577 |
PRIDEi | P51577 |
Genome annotation databases
Ensembli | ENSRNOT00000001752; ENSRNOP00000001752; ENSRNOG00000001300 |
GeneIDi | 29659 |
KEGGi | rno:29659 |
UCSCi | RGD:62073, rat |
Organism-specific databases
CTDi | 5025 |
RGDi | 62073, P2rx4 |
Phylogenomic databases
eggNOGi | ENOG502QSUI, Eukaryota |
GeneTreei | ENSGT01010000222290 |
HOGENOMi | CLU_034469_2_0_1 |
InParanoidi | P51577 |
OMAi | RAASHCV |
OrthoDBi | 1128763at2759 |
PhylomeDBi | P51577 |
TreeFami | TF328633 |
Enzyme and pathway databases
Reactomei | R-RNO-139853, Elevation of cytosolic Ca2+ levels R-RNO-418346, Platelet homeostasis |
Miscellaneous databases
EvolutionaryTracei | P51577 |
PROi | PR:P51577 |
Gene expression databases
Bgeei | ENSRNOG00000001300, Expressed in pancreas and 21 other tissues |
Genevisiblei | P51577, RN |
Family and domain databases
Gene3Di | 2.60.490.10, 1 hit |
IDEALi | IID50123 |
InterProi | View protein in InterPro IPR003047, P2X4_purnocptor IPR027309, P2X_extracellular_dom_sf IPR001429, P2X_purnocptor |
PIRSFi | PIRSF005713, P2X_purinoceptor, 1 hit |
PRINTSi | PR01311, P2X4RECEPTOR PR01307, P2XRECEPTOR |
TIGRFAMsi | TIGR00863, P2X, 1 hit |
PROSITEi | View protein in PROSITE PS01212, P2X_RECEPTOR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | P2RX4_RAT | |
Accessioni | P51577Primary (citable) accession number: P51577 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families