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Protein

P2X purinoceptor 4

Gene

P2rx4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.1 Publication

GO - Molecular functioni

  • ATP binding Source: RGD
  • cadherin binding Source: Ensembl
  • copper ion binding Source: BHF-UCL
  • drug binding Source: RGD
  • extracellularly ATP-gated cation channel activity Source: UniProtKB
  • identical protein binding Source: IntAct
  • ligand-gated calcium channel activity Source: ARUK-UCL
  • purinergic nucleotide receptor activity Source: BHF-UCL
  • signaling receptor binding Source: BHF-UCL
  • zinc ion binding Source: BHF-UCL

GO - Biological processi

  • apoptotic signaling pathway Source: Ensembl
  • behavioral response to pain Source: ARUK-UCL
  • calcium ion transmembrane transport Source: UniProtKB
  • cellular response to ATP Source: UniProtKB
  • cellular response to zinc ion Source: UniProtKB
  • membrane depolarization Source: Ensembl
  • negative regulation of cardiac muscle hypertrophy Source: Ensembl
  • neuronal action potential Source: RGD
  • positive regulation of blood vessel endothelial cell migration Source: Ensembl
  • positive regulation of calcium ion transport into cytosol Source: Ensembl
  • positive regulation of calcium-mediated signaling Source: Ensembl
  • positive regulation of endothelial cell chemotaxis Source: Ensembl
  • positive regulation of microglial cell migration Source: ARUK-UCL
  • positive regulation of protein kinase B signaling Source: ARUK-UCL
  • protein homooligomerization Source: RGD
  • regulation of blood pressure Source: Ensembl
  • regulation of cardiac muscle contraction Source: Ensembl
  • regulation of chemotaxis Source: ARUK-UCL
  • regulation of sodium ion transport Source: BHF-UCL
  • relaxation of cardiac muscle Source: Ensembl
  • response to axon injury Source: ARUK-UCL
  • response to fluid shear stress Source: Ensembl
  • response to ischemia Source: ARUK-UCL
  • sensory perception of pain Source: ARUK-UCL
  • sensory perception of touch Source: ARUK-UCL

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-139853 Elevation of cytosolic Ca2+ levels
R-RNO-418346 Platelet homeostasis

Names & Taxonomyi

Protein namesi
Recommended name:
P2X purinoceptor 4
Short name:
P2X4
Alternative name(s):
ATP receptor
Purinergic receptor
Gene namesi
Name:P2rx4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi62073 P2rx4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicSequence analysisAdd BLAST33
Transmembranei34 – 54Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini55 – 338ExtracellularSequence analysisAdd BLAST284
Transmembranei339 – 359Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini360 – 388CytoplasmicSequence analysisAdd BLAST29

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi249E → K: Restores antagonism by PPADS. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2818
GuidetoPHARMACOLOGYi481

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001615541 – 388P2X purinoceptor 4Add BLAST388

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi75N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi110N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi116 ↔ 165By similarity
Disulfide bondi126 ↔ 149By similarity
Disulfide bondi132 ↔ 159By similarity
Glycosylationi153N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi184N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi199N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi208N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi217 ↔ 227By similarity
Disulfide bondi261 ↔ 270By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP51577
PRIDEiP51577

PTM databases

PhosphoSitePlusiP51577

Expressioni

Tissue specificityi

Widespread distribution in the brain. Also expressed in epithelial cells.1 Publication

Gene expression databases

BgeeiENSRNOG00000001300
GenevisibleiP51577 RN

Interactioni

Subunit structurei

Functional P2XRs are organized as homomeric and heteromeric trimers.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • cadherin binding Source: Ensembl
  • identical protein binding Source: IntAct
  • signaling receptor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi248281, 2 interactors
DIPiDIP-48411N
IntActiP51577, 2 interactors
MINTiP51577
STRINGi10116.ENSRNOP00000001752

Chemistry databases

BindingDBiP51577

Structurei

Secondary structure

1388
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi114 – 117Combined sources4
Turni129 – 131Combined sources3
Turni139 – 141Combined sources3
Beta strandi143 – 150Combined sources8
Beta strandi153 – 155Combined sources3
Beta strandi158 – 163Combined sources6
Beta strandi379 – 381Combined sources3

3D structure databases

SMRiP51577
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP51577

Family & Domainsi

Sequence similaritiesi

Belongs to the P2X receptor family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFJF Eukaryota
ENOG410XR0C LUCA
GeneTreeiENSGT00390000016028
HOGENOMiHOG000232042
HOVERGENiHBG053086
InParanoidiP51577
KOiK05218
OMAiTMINVGS
OrthoDBiEOG091G0A2U
PhylomeDBiP51577
TreeFamiTF328633

Family and domain databases

Gene3Di2.60.490.10, 1 hit
InterProiView protein in InterPro
IPR003047 P2X4_purnocptor
IPR027309 P2X_extracellular_dom_sf
IPR001429 P2X_purnocptor
PANTHERiPTHR10125 PTHR10125, 1 hit
PfamiView protein in Pfam
PF00864 P2X_receptor, 1 hit
PIRSFiPIRSF005713 P2X_purinoceptor, 1 hit
PRINTSiPR01311 P2X4RECEPTOR
PR01307 P2XRECEPTOR
TIGRFAMsiTIGR00863 P2X, 1 hit
PROSITEiView protein in PROSITE
PS01212 P2X_RECEPTOR, 1 hit

Sequencei

Sequence statusi: Complete.

P51577-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGCCSVLGS FLFEYDTPRI VLIRSRKVGL MNRAVQLLIL AYVIGWVFVW
60 70 80 90 100
EKGYQETDSV VSSVTTKAKG VAVTNTSQLG FRIWDVADYV IPAQEENSLF
110 120 130 140 150
IMTNMIVTVN QTQSTCPEIP DKTSICNSDA DCTPGSVDTH SSGVATGRCV
160 170 180 190 200
PFNESVKTCE VAAWCPVEND VGVPTPAFLK AAENFTLLVK NNIWYPKFNF
210 220 230 240 250
SKRNILPNIT TSYLKSCIYN AQTDPFCPIF RLGTIVEDAG HSFQEMAVEG
260 270 280 290 300
GIMGIQIKWD CNLDRAASLC LPRYSFRRLD TRDLEHNVSP GYNFRFAKYY
310 320 330 340 350
RDLAGKEQRT LTKAYGIRFD IIVFGKAGKF DIIPTMINVG SGLALLGVAT
360 370 380
VLCDVIVLYC MKKKYYYRDK KYKYVEDYEQ GLSGEMNQ
Length:388
Mass (Da):43,501
Last modified:October 1, 1996 - v1
Checksum:iC7B0FC9A94EF8D9A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136 – 137SV → LR in AAC52380 (PubMed:8551329).Curated2
Sequence conflicti237E → G in CAA61037 (PubMed:8598206).Curated1
Sequence conflicti305G → A in CAA62607 (PubMed:7498461).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32497 mRNA Translation: AAC52380.1
X91200 mRNA Translation: CAA62607.1
U47031 mRNA Translation: AAA99777.1
X87763 mRNA Translation: CAA61037.1
X93565 mRNA Translation: CAA63778.1
BC078792 mRNA Translation: AAH78792.1
PIRiJC6137
RefSeqiNP_113782.1, NM_031594.1
UniGeneiRn.7176

Genome annotation databases

EnsembliENSRNOT00000001752; ENSRNOP00000001752; ENSRNOG00000001300
GeneIDi29659
KEGGirno:29659
UCSCiRGD:62073 rat

Similar proteinsi

Entry informationi

Entry nameiP2RX4_RAT
AccessioniPrimary (citable) accession number: P51577
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 18, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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