P2X purinoceptor 4

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• ATP binding Source: RGD <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "Histidine 140 plays a key role in the inhibitory modulation of the P2X4 nucleotide receptor by copper but not zinc."
    Coddou C., Morales B., Gonzalez J., Grauso M., Gordillo F., Bull P., Rassendren F., Huidobro-Toro J.P.
    J Biol Chem 278:36777-36785(2003) [PubMed] [Europe PMC] [Abstract]
  • "Characterization of three types of ATP-activated current in relation to P2X subunits in rat trigeminal ganglion neurons."
    Luo J., Yin G.F., Gu Y.Z., Liu Y., Dai J.P., Li C., Li Z.W.
    Brain Res 1115:9-15(2006) [PubMed] [Europe PMC] [Abstract]
• cadherin binding Source: RGD
• copper ion binding Source: BHF-UCLInferred from direct assayⁱ
  • "Histidine 140 plays a key role in the inhibitory modulation of the P2X4 nucleotide receptor by copper but not zinc."
    Coddou C., Morales B., Gonzalez J., Grauso M., Gordillo F., Bull P., Rassendren F., Huidobro-Toro J.P.
    J Biol Chem 278:36777-36785(2003) [PubMed] [Europe PMC] [Abstract]
• extracellularly ATP-gated cation channel activity Source: UniProtKBInferred from direct assayⁱ
  • Ref.1

    "A novel neuronal P2x ATP receptor ion channel with widespread distribution in the brain."
    Seguela P., Haghighi A., Soghomonian J.J., Cooper E.
    J. Neurosci. 16:448-455(1996) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
• identical protein binding Source: IntAct <p>Inferred from Physical Interaction</p> <p>Covers physical interactions between the gene product of interest and another molecule (or ion, or complex).</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#ipi">GO evidence code guide</a></p> Inferred from physical interactionⁱ
  • "Direct observation of ATP-induced conformational changes in single P2X(4) receptors."
    Shinozaki Y., Sumitomo K., Tsuda M., Koizumi S., Inoue K., Torimitsu K.
    PLoS Biol 7:e1000103-e1000103(2009) [PubMed] [Europe PMC] [Abstract]
• ligand-gated calcium channel activity Source: ARUK-UCL <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "Involvement of P2X4 and P2Y12 receptors in ATP-induced microglial chemotaxis."
    Ohsawa K., Irino Y., Nakamura Y., Akazawa C., Inoue K., Kohsaka S.
    Glia 55:604-616(2007) [PubMed] [Europe PMC] [Abstract]
• purinergic nucleotide receptor activity Source: BHF-UCLInferred from direct assayⁱ
  • "Histidine 140 plays a key role in the inhibitory modulation of the P2X4 nucleotide receptor by copper but not zinc."
    Coddou C., Morales B., Gonzalez J., Grauso M., Gordillo F., Bull P., Rassendren F., Huidobro-Toro J.P.
    J Biol Chem 278:36777-36785(2003) [PubMed] [Europe PMC] [Abstract]
• signaling receptor binding Source: BHF-UCLInferred from physical interactionⁱ
  • "Evidence for functional P2X4/P2X7 heteromeric receptors."
    Guo C., Masin M., Qureshi O.S., Murrell-Lagnado R.D.
    Mol Pharmacol 72:1447-1456(2007) [PubMed] [Europe PMC] [Abstract]
• zinc ion binding Source: BHF-UCLInferred from direct assayⁱ
  • "Histidine 140 plays a key role in the inhibitory modulation of the P2X4 nucleotide receptor by copper but not zinc."
    Coddou C., Morales B., Gonzalez J., Grauso M., Gordillo F., Bull P., Rassendren F., Huidobro-Toro J.P.
    J Biol Chem 278:36777-36785(2003) [PubMed] [Europe PMC] [Abstract]

GO - Biological processⁱ

• apoptotic signaling pathway Source: RGD
• behavioral response to pain Source: ARUK-UCLInferred from mutant phenotypeⁱ
  • "P2X4 receptors induced in spinal microglia gate tactile allodynia after nerve injury."
    Tsuda M., Shigemoto-Mogami Y., Koizumi S., Mizokoshi A., Kohsaka S., Salter M.W., Inoue K.
    Nature 424:778-783(2003) [PubMed] [Europe PMC] [Abstract]
• calcium ion transmembrane transport Source: UniProtKBInferred from direct assayⁱ
  • Ref.1

    "A novel neuronal P2x ATP receptor ion channel with widespread distribution in the brain."
    Seguela P., Haghighi A., Soghomonian J.J., Cooper E.
    J. Neurosci. 16:448-455(1996) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
• calcium ion transport Source: RGD
• cellular response to ATP Source: UniProtKBInferred from direct assayⁱ
  • Ref.1

    "A novel neuronal P2x ATP receptor ion channel with widespread distribution in the brain."
    Seguela P., Haghighi A., Soghomonian J.J., Cooper E.
    J. Neurosci. 16:448-455(1996) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
• cellular response to zinc ion Source: UniProtKBInferred from direct assayⁱ
  • Ref.1

    "A novel neuronal P2x ATP receptor ion channel with widespread distribution in the brain."
    Seguela P., Haghighi A., Soghomonian J.J., Cooper E.
    J. Neurosci. 16:448-455(1996) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
• excitatory postsynaptic potential Source: RGD
• inorganic cation transmembrane transport Source: RGDInferred from direct assayⁱ
  • "Functional properties of internalization-deficient P2X4 receptors reveal a novel mechanism of ligand-gated channel facilitation by ivermectin."
    Toulme E., Soto F., Garret M., Boue-Grabot E.
    Mol Pharmacol 69:576-587(2006) [PubMed] [Europe PMC] [Abstract]
• ion transmembrane transport Source: RGD
• membrane depolarization Source: RGD
• negative regulation of cardiac muscle hypertrophy Source: RGD
• neuronal action potential Source: RGDInferred from direct assayⁱ
  • "Histidine 140 plays a key role in the inhibitory modulation of the P2X4 nucleotide receptor by copper but not zinc."
    Coddou C., Morales B., Gonzalez J., Grauso M., Gordillo F., Bull P., Rassendren F., Huidobro-Toro J.P.
    J Biol Chem 278:36777-36785(2003) [PubMed] [Europe PMC] [Abstract]
  • "Characterization of three types of ATP-activated current in relation to P2X subunits in rat trigeminal ganglion neurons."
    Luo J., Yin G.F., Gu Y.Z., Liu Y., Dai J.P., Li C., Li Z.W.
    Brain Res 1115:9-15(2006) [PubMed] [Europe PMC] [Abstract]
• nitric oxide biosynthetic process Source: RGD
• positive regulation of blood vessel endothelial cell migration Source: RGD
• positive regulation of calcium ion transport into cytosol Source: RGD
• positive regulation of calcium-mediated signaling Source: RGD
• positive regulation of endothelial cell chemotaxis Source: RGD
• positive regulation of microglial cell migration Source: ARUK-UCLInferred from mutant phenotypeⁱ
  • "Involvement of P2X4 and P2Y12 receptors in ATP-induced microglial chemotaxis."
    Ohsawa K., Irino Y., Nakamura Y., Akazawa C., Inoue K., Kohsaka S.
    Glia 55:604-616(2007) [PubMed] [Europe PMC] [Abstract]
• positive regulation of protein kinase B signaling Source: ARUK-UCLInferred from mutant phenotypeⁱ
  • "Involvement of P2X4 and P2Y12 receptors in ATP-induced microglial chemotaxis."
    Ohsawa K., Irino Y., Nakamura Y., Akazawa C., Inoue K., Kohsaka S.
    Glia 55:604-616(2007) [PubMed] [Europe PMC] [Abstract]
• purinergic nucleotide receptor signaling pathway Source: RGD
• regulation of blood pressure Source: RGD
• regulation of cardiac muscle contraction Source: RGD
• regulation of chemotaxis Source: ARUK-UCLInferred from mutant phenotypeⁱ
  • "Involvement of P2X4 and P2Y12 receptors in ATP-induced microglial chemotaxis."
    Ohsawa K., Irino Y., Nakamura Y., Akazawa C., Inoue K., Kohsaka S.
    Glia 55:604-616(2007) [PubMed] [Europe PMC] [Abstract]
• regulation of sodium ion transport Source: BHF-UCLInferred from direct assayⁱ
  • "Evidence for functional P2X4/P2X7 heteromeric receptors."
    Guo C., Masin M., Qureshi O.S., Murrell-Lagnado R.D.
    Mol Pharmacol 72:1447-1456(2007) [PubMed] [Europe PMC] [Abstract]
• relaxation of cardiac muscle Source: RGD
• response to ATP Source: RGD
• response to axon injury Source: ARUK-UCLInferred from mutant phenotypeⁱ
  • "P2X4 receptors induced in spinal microglia gate tactile allodynia after nerve injury."
    Tsuda M., Shigemoto-Mogami Y., Koizumi S., Mizokoshi A., Kohsaka S., Salter M.W., Inoue K.
    Nature 424:778-783(2003) [PubMed] [Europe PMC] [Abstract]
• response to fluid shear stress Source: RGD
• response to ischemia Source: ARUK-UCLInferred from mutant phenotypeⁱ
  • "Up-regulation of P2X2, P2X4 receptor and ischemic cell death: prevention by P2 antagonists."
    Cavaliere F., Florenzano F., Amadio S., Fusco F.R., Viscomi M.T., D'Ambrosi N., Vacca F., Sancesario G., Bernardi G., Molinari M., Volonte C.
    Neuroscience 120:85-98(2003) [PubMed] [Europe PMC] [Abstract]
• sensory perception of pain Source: ARUK-UCLInferred from mutant phenotypeⁱ
  • "P2X4 receptors induced in spinal microglia gate tactile allodynia after nerve injury."
    Tsuda M., Shigemoto-Mogami Y., Koizumi S., Mizokoshi A., Kohsaka S., Salter M.W., Inoue K.
    Nature 424:778-783(2003) [PubMed] [Europe PMC] [Abstract]
• sensory perception of touch Source: ARUK-UCLInferred from mutant phenotypeⁱ
  • "P2X4 receptors induced in spinal microglia gate tactile allodynia after nerve injury."
    Tsuda M., Shigemoto-Mogami Y., Koizumi S., Mizokoshi A., Kohsaka S., Salter M.W., Inoue K.
    Nature 424:778-783(2003) [PubMed] [Europe PMC] [Abstract]
• signal transduction Source: RGD
• vasodilation Source: RGD

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

Topology

Keywords - Cellular componentⁱ

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechⁱ

Mutagenesis

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Amino acid modifications

Keywords - PTMⁱ

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressionⁱ

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityⁱ

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsⁱ

GO - Molecular functionⁱ

• cadherin binding Source: RGD
• identical protein binding Source: IntActInferred from physical interactionⁱ
  • "Direct observation of ATP-induced conformational changes in single P2X(4) receptors."
    Shinozaki Y., Sumitomo K., Tsuda M., Koizumi S., Inoue K., Torimitsu K.
    PLoS Biol 7:e1000103-e1000103(2009) [PubMed] [Europe PMC] [Abstract]
• signaling receptor binding Source: BHF-UCLInferred from physical interactionⁱ
  • "Evidence for functional P2X4/P2X7 heteromeric receptors."
    Guo C., Masin M., Qureshi O.S., Murrell-Lagnado R.D.
    Mol Pharmacol 72:1447-1456(2007) [PubMed] [Europe PMC] [Abstract]

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

        10         20         30         40         50
MAGCCSVLGS FLFEYDTPRI VLIRSRKVGL MNRAVQLLIL AYVIGWVFVW 
        60         70         80         90        100
EKGYQETDSV VSSVTTKAKG VAVTNTSQLG FRIWDVADYV IPAQEENSLF 
       110        120        130        140        150
IMTNMIVTVN QTQSTCPEIP DKTSICNSDA DCTPGSVDTH SSGVATGRCV 
       160        170        180        190        200
PFNESVKTCE VAAWCPVEND VGVPTPAFLK AAENFTLLVK NNIWYPKFNF 
       210        220        230        240        250
SKRNILPNIT TSYLKSCIYN AQTDPFCPIF RLGTIVEDAG HSFQEMAVEG 
       260        270        280        290        300
GIMGIQIKWD CNLDRAASLC LPRYSFRRLD TRDLEHNVSP GYNFRFAKYY 
       310        320        330        340        350
RDLAGKEQRT LTKAYGIRFD IIVFGKAGKF DIIPTMINVG SGLALLGVAT 
       360        370        380 
VLCDVIVLYC MKKKYYYRDK KYKYVEDYEQ GLSGEMNQ              

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references
2. SIMILARITY comments
   Index of protein domains and families