Rho

Functionⁱ

Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (By similarity). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (By similarity).By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Siteⁱ	113	Plays an important role in the conformation switch to the active conformationBy similarity	1
Metal bindingⁱ	201	ZincBy similarity	1
Metal bindingⁱ	279	ZincBy similarity	1

GO - Molecular functionⁱ

11-cis retinal binding Source: UniProtKB
G-protein coupled photoreceptor activity Source: UniProtKB
metal ion binding Source: UniProtKB-KW
retinal binding
Source: RGD
Inferred from direct assayⁱ
- 2525480
- 8814134
spectrin binding
Source: MGI
Inferred from direct assayⁱ
- 23704327

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

absorption of visible light Source: AgBase
protein-chromophore linkage Source: UniProtKB-KW
red, far-red light phototransduction
Source: RGD
Inferred from direct assayⁱ
- 2525480
rhodopsin mediated signaling pathway
Source: RGD
Inferred from direct assayⁱ
- 8814134
visual perception Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	G-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer
Biological process	Sensory transduction, Vision
Ligand	Chromophore, Metal-binding, Zinc

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Rhodopsin
Gene namesⁱ	Name:Rho
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Unplaced

Organism-specific databases

Rat genome database More...RGDⁱ	3573 Rho

RGDⁱ

3573 Rho

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 36	ExtracellularCuratedAdd BLAST	36
Transmembraneⁱ	37 – 61	Helical; Name=1By similarityAdd BLAST	25
Topological domainⁱ	62 – 73	CytoplasmicCuratedAdd BLAST	12
Transmembraneⁱ	74 – 96	Helical; Name=2By similarityAdd BLAST	23
Topological domainⁱ	97 – 110	ExtracellularCuratedAdd BLAST	14
Transmembraneⁱ	111 – 133	Helical; Name=3By similarityAdd BLAST	23
Topological domainⁱ	134 – 152	CytoplasmicCuratedAdd BLAST	19
Transmembraneⁱ	153 – 173	Helical; Name=4By similarityAdd BLAST	21
Topological domainⁱ	174 – 202	ExtracellularCuratedAdd BLAST	29
Transmembraneⁱ	203 – 224	Helical; Name=5By similarityAdd BLAST	22
Topological domainⁱ	225 – 252	CytoplasmicCuratedAdd BLAST	28
Transmembraneⁱ	253 – 274	Helical; Name=6By similarityAdd BLAST	22
Topological domainⁱ	275 – 286	ExtracellularCuratedAdd BLAST	12
Transmembraneⁱ	287 – 308	Helical; Name=7By similarityAdd BLAST	22
Topological domainⁱ	309 – 348	CytoplasmicCuratedAdd BLAST	40

Keywords - Cellular componentⁱ

Cell projection, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000197708	1 – 348	RhodopsinAdd BLAST		348

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	1	N-acetylmethionineBy similarity	1
Glycosylationⁱ	2	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	15	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	110 ↔ 187	PROSITE-ProRule annotation
Modified residueⁱ	296	N6-(retinylidene)lysineBy similarity	1
Lipidationⁱ	322	S-palmitoyl cysteineBy similarity	1
Lipidationⁱ	323	S-palmitoyl cysteineBy similarity	1
Modified residueⁱ	334	PhosphoserineBy similarity	1
Modified residueⁱ	336	PhosphothreonineBy similarity	1
Modified residueⁱ	338	PhosphoserineBy similarity	1
Modified residueⁱ	340	PhosphothreonineBy similarity	1
Modified residueⁱ	342	PhosphothreonineBy similarity	1
Modified residueⁱ	343	PhosphoserineBy similarity	1

Post-translational modificationⁱ

Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.By similarity

Contains one covalently linked retinal chromophore. Upon light absorption, the covalently bound 11-cis-retinal is converted to all-trans-retinal. After hydrolysis of the Schiff base and release of the covalently bound all-trans-retinal, active rhodopsin is regenerated by binding of a fresh molecule of 11-cis-retinal.By similarity

Keywords - PTMⁱ

Acetylation, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbⁱ	P51489
PRIDEⁱ	P51489

PTM databases

iPTMnetⁱ	P51489
PhosphoSitePlusⁱ	P51489

Interactionⁱ

Subunit structureⁱ

Homodimer. Interacts (phosphorylated form) with SAG. Interacts with GNAT1, Interacts with GNAT3. SAG and G-proteins compete for a common binding site. Interacts with GRK1 (By similarity).By similarity

GO - Molecular functionⁱ

spectrin binding
Source: MGI
Inferred from direct assayⁱ
- 23704327

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	10116.ENSRNOP00000061473

STRINGⁱ

10116.ENSRNOP00000061473

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P51489
SMRⁱ	P51489
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	330 – 348	Interaction with SAGBy similarityAdd BLAST		19

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	134 – 136	'Ionic lock' involved in activated form stabilizationBy similarity		3

Sequence similaritiesⁱ

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.PROSITE-ProRule annotation

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG3656 Eukaryota ENOG410XRW9 LUCA
HOGENOMⁱ	HOG000253932
HOVERGENⁱ	HBG107442
InParanoidⁱ	P51489
KEGG Orthology (KO) More...KOⁱ	K04250
PhylomeDBⁱ	P51489
TreeFamⁱ	TF324998

Family and domain databases

InterProⁱ	View protein in InterPro IPR000276 GPCR_Rhodpsn IPR017452 GPCR_Rhodpsn_7TM IPR001760 Opsin IPR027430 Retinal_BS IPR000732 Rhodopsin IPR019477 Rhodopsin_N
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00001 7tm_1, 1 hit PF10413 Rhodopsin_N, 1 hit
PRINTSⁱ	PR00237 GPCRRHODOPSN PR00238 OPSIN PR00579 RHODOPSIN
PROSITEⁱ	View protein in PROSITE PS00237 G_PROTEIN_RECEP_F1_1, 1 hit PS50262 G_PROTEIN_RECEP_F1_2, 1 hit PS00238 OPSIN, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.ⁱShow all

P51489-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MNGTEGPNFY VPFSNITGVV RSPFEQPQYY LAEPWQFSML AAYMFLLIVL 
        60         70         80         90        100
GFPINFLTLY VTVQHKKLRT PLNYILLNLA VADLFMVFGG FTTTLYTSLH 
       110        120        130        140        150
GYFVFGPTGC NLEGFFATLG GEIGLWSLVV LAIERYVVVC KPMSNFRFGE 
       160        170        180        190        200
NHAIMGVAFT WVMALACAAP PLVGWSRYIP EGMQCSCGID YYTLKPEVNN 
       210        220        230        240        250
ESFVIYMFVV HFTIPMIVIF FCYGQLVFTV KEAAAQQQES ATTQKAEKEV 
       260        270        280        290        300
TRMVIIMVIF FLICWLPYAS VAMYIFTHQG SNFGPIFMTL PAFFAKTASI 
       310        320        330        340 
YNPIIYIMMN KQFRNCMLTS LCCGKNPLGD DEASATASKT ETSQVAPA

Length:348

Mass (Da):39,042

Last modified:October 1, 1996 - v1

Checksum:ⁱDA0F3F90C30984BC

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation
	A0A0G2JSY7	A0A0G2JSY7_RAT		Rhodopsin Rhodopsin	Rho rCG_29898	348	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	320	S → T in CAA87081 (Ref. 2) Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U22180 Genomic DNA Translation: AAA84439.1 Z46957 mRNA Translation: CAA87081.1
PIRⁱ	S51677
NCBI Reference Sequences More...RefSeqⁱ	NP_254276.1, NM_033441.1
UniGeneⁱ	Rn.92530

Genome annotation databases

GeneIDⁱ	24717
KEGGⁱ	rno:24717
UCSC genome browser More...UCSCⁱ	RGD:3573 rat

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P51489	Rhodopsin	)	HUMAN		348	UniRef90_P08100
Rhodopsin	)	BOVIN		348
Rhodopsin		SHEEP		348
Rhodopsin		SMICR		348
Rhodopsin		TRIMA		348
+343

Protein	Similar proteins		Species	Score	Length	Source
P51489	Rhodopsin	)	HUMAN		348	UniRef50_P08100
Rhodopsin		DELDE		348
Rhodopsin		SMICR		348
Rhodopsin		OTOCR		348
Rhodopsin	)	TURTR		348
+567

Entry informationⁱ

Entry nameⁱ	OPSD_RAT
Accessionⁱ	P51489Primary (citable) accession number: P51489
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
	Last sequence update:	October 1, 1996
	Last modified:	May 23, 2018
	This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P51489 (OPSD_RAT)

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Rhodopsin

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Endoplasmic reticulum

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Computationally mapped potential isoform sequencesi

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Computationally mapped potential isoform sequencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ