Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

D(4) dopamine receptor

Gene

Drd4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Activated by dopamine, but also by epinephrine and norepinephrine, and by numerous synthetic agonists and drugs. Agonist binding triggers signaling via G proteins that inhibit adenylyl cyclase (By similarity). Modulates the circadian rhythm of contrast sensitivity by regulating the rhythmic expression of NPAS2 in the retinal ganglion cells (PubMed:24048828).By similarity1 Publication

Caution

In contrast to human protein, does not interact with KLHL12 and is not ubiquitinated by the BCR(KLHL12) complex.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi77SodiumBy similarity1
Binding sitei112AgonistBy similarity1
Metal bindingi119SodiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processBiological rhythms
LigandMetal-binding, Sodium

Enzyme and pathway databases

ReactomeiR-MMU-390651 Dopamine receptors
R-MMU-418594 G alpha (i) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
D(4) dopamine receptor
Alternative name(s):
D(2C) dopamine receptor
Dopamine D4 receptor
Gene namesi
Name:Drd4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:94926 Drd4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 34ExtracellularBy similarityAdd BLAST34
Transmembranei35 – 57Helical; Name=1By similarityAdd BLAST23
Topological domaini58 – 67CytoplasmicBy similarity10
Transmembranei68 – 90Helical; Name=2By similarityAdd BLAST23
Topological domaini91 – 106ExtracellularBy similarityAdd BLAST16
Transmembranei107 – 128Helical; Name=3By similarityAdd BLAST22
Topological domaini129 – 146CytoplasmicBy similarityAdd BLAST18
Transmembranei147 – 170Helical; Name=4By similarityAdd BLAST24
Topological domaini171 – 186ExtracellularBy similarityAdd BLAST16
Transmembranei187 – 208Helical; Name=5By similarityAdd BLAST22
Topological domaini209 – 314CytoplasmicBy similarityAdd BLAST106
Transmembranei315 – 337Helical; Name=6By similarityAdd BLAST23
Topological domaini338 – 346ExtracellularBy similarity9
Transmembranei347 – 369Helical; Name=7By similarityAdd BLAST23
Topological domaini370 – 387CytoplasmicBy similarityAdd BLAST18

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show a significant reduction in daytime contrast sensitivity.1 Publication

Chemistry databases

ChEMBLiCHEMBL2574

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000694021 – 387D(4) dopamine receptorAdd BLAST387

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi3N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi105 ↔ 180PROSITE-ProRule annotation
Disulfide bondi340 ↔ 343By similarity
Lipidationi387S-palmitoyl cysteineBy similarity1

Post-translational modificationi

Polyubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex: polyubiquitination does not lead to degradation of DRD4 protein.By similarity
Palmitoylated. Palmitoylation of the C-terminal Cys is important for normal expression at the cell membrane.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Ubl conjugation

Proteomic databases

PaxDbiP51436
PRIDEiP51436

PTM databases

iPTMnetiP51436
PhosphoSitePlusiP51436

Expressioni

Tissue specificityi

Detected in olfactory bulb, hypothalamus, olfactory tubercle, brainstem and striatum.1 Publication

Gene expression databases

BgeeiENSMUSG00000025496 Expressed in 68 organ(s), highest expression level in retina
CleanExiMM_DRD4
GenevisibleiP51436 MM

Interactioni

Subunit structurei

Forms homo- and heterooligomers with DRD2. D4.7 allele exhibits higher affinity for homodimers compared to DRD2 heterodimers, while alleles D42. and 4.4 have similar affinities for both. The interaction with DRD2 may modulate agonist-induced downstream signaling (By similarity). Interacts with CLIC6 (By similarity). Interacts with GPRASP1. May interact with ADORA2A. Interacts with KLHL12 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-61454N
IntActiP51436, 3 interactors
STRINGi10090.ENSMUSP00000026569

Chemistry databases

BindingDBiP51436

Structurei

3D structure databases

ProteinModelPortaliP51436
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00890000139331
HOGENOMiHOG000239242
HOVERGENiHBG106962
InParanoidiP51436
KOiK04147
OMAiVVHITQA
OrthoDBiEOG091G06VI
PhylomeDBiP51436
TreeFamiTF334382

Family and domain databases

InterProiView protein in InterPro
IPR002185 Dopamine_D4_rcpt
IPR000929 Dopamine_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PANTHERiPTHR44250:SF1 PTHR44250:SF1, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00569 DOPAMINED4R
PR00242 DOPAMINER
PR00237 GPCRRHODOPSN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

P51436-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGNSSATEDG GLLAGRGPES LGTGAGLGGA GAAALVGGVL LIGLVLAGNS
60 70 80 90 100
LVCVSVASER TLQTPTNYFI VSLAAADLLL AVLVLPLFVY SEVQGGVWLL
110 120 130 140 150
SPRLCDTLMA MDVMLCTASI FNLCAISVDR FVAVTVPLRY NQQGQCQLLL
160 170 180 190 200
IAATWLLSAA VASPVVCGLN DVPGRDPAVC CLENRDYVVY SSVCSFFLPC
210 220 230 240 250
PLMLLLYWAT FRGLRRWEAA RHTKLHSRAP RRPSGPGPPV SDPTQGPFFP
260 270 280 290 300
DCPPPLPSLR TSPSDSSRPE SELSQRPCSP GCLLADAALP QPPEPSSRRR
310 320 330 340 350
RGAKITGRER KAMRVLPVVV GAFLVCWTPF FVVHITRALC PACFVSPRLV
360 370 380
SAVTWLGYVN SALNPIIYTI FNAEFRSVFR KTLRLRC
Length:387
Mass (Da):41,487
Last modified:October 1, 1996 - v1
Checksum:iBEA306D5E8AA02E9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1M → L in BAC31893 (PubMed:16141072).Curated1
Sequence conflicti19E → K in BAC31893 (PubMed:16141072).Curated1
Sequence conflicti47A → T in AAB50730 (PubMed:8584230).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19880 Genomic DNA Translation: AAC52190.1
S80929
, S80920, S80927, S80928 Genomic DNA Translation: AAB50730.1
AK044379 mRNA Translation: BAC31893.1
CH466531 Genomic DNA Translation: EDL18041.1
BC016086 mRNA Translation: AAH16086.1
BC051421 mRNA Translation: AAH51421.2
CCDSiCCDS22007.1
PIRiI49246
RefSeqiNP_031904.1, NM_007878.2
UniGeneiMm.41075

Genome annotation databases

EnsembliENSMUST00000026569; ENSMUSP00000026569; ENSMUSG00000025496
GeneIDi13491
KEGGimmu:13491
UCSCiuc009kkm.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19880 Genomic DNA Translation: AAC52190.1
S80929
, S80920, S80927, S80928 Genomic DNA Translation: AAB50730.1
AK044379 mRNA Translation: BAC31893.1
CH466531 Genomic DNA Translation: EDL18041.1
BC016086 mRNA Translation: AAH16086.1
BC051421 mRNA Translation: AAH51421.2
CCDSiCCDS22007.1
PIRiI49246
RefSeqiNP_031904.1, NM_007878.2
UniGeneiMm.41075

3D structure databases

ProteinModelPortaliP51436
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61454N
IntActiP51436, 3 interactors
STRINGi10090.ENSMUSP00000026569

Chemistry databases

BindingDBiP51436
ChEMBLiCHEMBL2574

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP51436
PhosphoSitePlusiP51436

Proteomic databases

PaxDbiP51436
PRIDEiP51436

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026569; ENSMUSP00000026569; ENSMUSG00000025496
GeneIDi13491
KEGGimmu:13491
UCSCiuc009kkm.1 mouse

Organism-specific databases

CTDi1815
MGIiMGI:94926 Drd4

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00890000139331
HOGENOMiHOG000239242
HOVERGENiHBG106962
InParanoidiP51436
KOiK04147
OMAiVVHITQA
OrthoDBiEOG091G06VI
PhylomeDBiP51436
TreeFamiTF334382

Enzyme and pathway databases

ReactomeiR-MMU-390651 Dopamine receptors
R-MMU-418594 G alpha (i) signalling events

Miscellaneous databases

PROiPR:P51436
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025496 Expressed in 68 organ(s), highest expression level in retina
CleanExiMM_DRD4
GenevisibleiP51436 MM

Family and domain databases

InterProiView protein in InterPro
IPR002185 Dopamine_D4_rcpt
IPR000929 Dopamine_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PANTHERiPTHR44250:SF1 PTHR44250:SF1, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00569 DOPAMINED4R
PR00242 DOPAMINER
PR00237 GPCRRHODOPSN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDRD4_MOUSE
AccessioniPrimary (citable) accession number: P51436
Secondary accession number(s): O35838, Q7TT80, Q8BXS4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again