UniProtKB - P51175 (PPOX_MOUSE)
Protein
Protoporphyrinogen oxidase
Gene
Ppox
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication
Catalytic activityi
- EC:1.3.3.41 Publication
Cofactori
FADBy similarityNote: Binds 1 FAD per subunit.By similarity
Activity regulationi
Inhibited by acifluorfen.1 Publication
: protoporphyrin-IX biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.Proteins known to be involved in this subpathway in this organism are:
- Protoporphyrinogen oxidase (Ppox), Protoporphyrinogen oxidase (Ppox), Protoporphyrinogen oxidase (Ppox)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 42 | FAD; via amide nitrogenBy similarity | 1 | |
Binding sitei | 257 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 449 | FAD; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 9 – 14 | FADBy similarity | 6 | |
Nucleotide bindingi | 34 – 35 | FADBy similarity | 2 | |
Nucleotide bindingi | 57 – 60 | FADBy similarity | 4 | |
Nucleotide bindingi | 454 – 456 | FADBy similarity | 3 |
GO - Molecular functioni
- oxidoreductase activity Source: GO_Central
- oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB
GO - Biological processi
- heme biosynthetic process Source: MGI
- oxidation-reduction process Source: UniProtKB
- porphyrin-containing compound biosynthetic process Source: UniProtKB
- protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
- protoporphyrinogen IX metabolic process Source: MGI
- response to drug Source: Ensembl
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Heme biosynthesis, Porphyrin biosynthesis |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
Reactomei | R-MMU-189451, Heme biosynthesis |
UniPathwayi | UPA00251;UER00324 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Ppox |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104968, Ppox |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Intermembrane side 1 Publication
Mitochondrion
- integral component of mitochondrial inner membrane Source: MGI
- intrinsic component of mitochondrial inner membrane Source: UniProtKB
- mitochondrial membrane Source: UniProtKB
- mitochondrion Source: MGI
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000135272 | 1 – 477 | Protoporphyrinogen oxidaseAdd BLAST | 477 |
Proteomic databases
EPDi | P51175 |
MaxQBi | P51175 |
PaxDbi | P51175 |
PeptideAtlasi | P51175 |
PRIDEi | P51175 |
PTM databases
iPTMneti | P51175 |
PhosphoSitePlusi | P51175 |
Expressioni
Inductioni
During erythroid differentiation.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000062729, Expressed in fetal liver hematopoietic progenitor cell and 295 other tissues |
Genevisiblei | P51175, MM |
Interactioni
Subunit structurei
Monomer. Homodimer (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 202334, 2 interactors |
IntActi | P51175, 4 interactors |
STRINGi | 10090.ENSMUSP00000072863 |
Miscellaneous databases
RNActi | P51175, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Protoporphyrinogen oxidase subfamily.Curated
Phylogenomic databases
eggNOGi | KOG1276, Eukaryota |
GeneTreei | ENSGT00390000008744 |
HOGENOMi | CLU_009629_2_1_1 |
InParanoidi | P51175 |
OMAi | IFDSCCF |
OrthoDBi | 1578484at2759 |
PhylomeDBi | P51175 |
TreeFami | TF323479 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR002937, Amino_oxidase IPR036188, FAD/NAD-bd_sf IPR004572, Protoporphyrinogen_oxidase |
Pfami | View protein in Pfam PF01593, Amino_oxidase, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
TIGRFAMsi | TIGR00562, proto_IX_ox, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P51175-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGRTVIVLGG GISGLAASYH LIRGPSPPKV ILVEGSKRLG GWIRSIRGSD
60 70 80 90 100
GAIFELGPRG IRPAGALGAR TLLLVSELGL ESEVLPVRGD HPAAQNRFLY
110 120 130 140 150
VGGTLHPLPS GLRGLLRPSP PFSKPLFWAG LRELLKPRGK EPDETVHSFA
160 170 180 190 200
QRRLGPEVAS LAMDSLCRGV FAGNSRELSI RSCFPSLFQA EQTHRSILLG
210 220 230 240 250
LLLGAGQSPQ PDSSLIRQAR AERWSQWSLR GGLEVLPQAL HNHLASKGVT
260 270 280 290 300
VLSGQPVCGL SLQPEGRWKV SLGDSSLEAD HIISAIPASE LSKLLPAEAA
310 320 330 340 350
PLARILSTIK AVSVAVVNLQ YRGACLPVQG FGHLVPSSED PTVLGIVYDS
360 370 380 390 400
VAFPEQDGNP PSLRVTVMLG GYWLQKLKAA GHQLSPELFQ QQAQEAAATQ
410 420 430 440 450
LGLKEPPSHC LVHLHKNCIP QYTIGHWQKL DSAMQFLTAQ RLPLTLAGAS
460 470
YEGVAVNDCI ESGRQAAVAV LGTESNS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0A6YX50 | A0A0A6YX50_MOUSE | Protoporphyrinogen oxidase | Ppox | 389 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 64 | A → T (PubMed:7607249).Curated | 1 | |
Sequence conflicti | 66 | A → P (PubMed:7607249).Curated | 1 | |
Sequence conflicti | 108 | L → S in BAA08126 (PubMed:7607249).Curated | 1 | |
Sequence conflicti | 176 | R → Q in AAH02047 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 427 | W → C in BAA08126 (PubMed:7607249).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25114 mRNA Translation: AAA96003.1 D45185 mRNA Translation: BAA08126.1 BC002047 mRNA Translation: AAH02047.1 |
CCDSi | CCDS15487.1 |
PIRi | S65684 S68367 |
RefSeqi | NP_032937.1, NM_008911.2 XP_006496770.1, XM_006496707.3 |
Genome annotation databases
Ensembli | ENSMUST00000073120; ENSMUSP00000072863; ENSMUSG00000062729 |
GeneIDi | 19044 |
KEGGi | mmu:19044 |
UCSCi | uc007dns.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25114 mRNA Translation: AAA96003.1 D45185 mRNA Translation: BAA08126.1 BC002047 mRNA Translation: AAH02047.1 |
CCDSi | CCDS15487.1 |
PIRi | S65684 S68367 |
RefSeqi | NP_032937.1, NM_008911.2 XP_006496770.1, XM_006496707.3 |
3D structure databases
SMRi | P51175 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202334, 2 interactors |
IntActi | P51175, 4 interactors |
STRINGi | 10090.ENSMUSP00000072863 |
PTM databases
iPTMneti | P51175 |
PhosphoSitePlusi | P51175 |
Proteomic databases
EPDi | P51175 |
MaxQBi | P51175 |
PaxDbi | P51175 |
PeptideAtlasi | P51175 |
PRIDEi | P51175 |
Protocols and materials databases
Antibodypediai | 34299, 252 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000073120; ENSMUSP00000072863; ENSMUSG00000062729 |
GeneIDi | 19044 |
KEGGi | mmu:19044 |
UCSCi | uc007dns.1, mouse |
Organism-specific databases
CTDi | 5498 |
MGIi | MGI:104968, Ppox |
Phylogenomic databases
eggNOGi | KOG1276, Eukaryota |
GeneTreei | ENSGT00390000008744 |
HOGENOMi | CLU_009629_2_1_1 |
InParanoidi | P51175 |
OMAi | IFDSCCF |
OrthoDBi | 1578484at2759 |
PhylomeDBi | P51175 |
TreeFami | TF323479 |
Enzyme and pathway databases
UniPathwayi | UPA00251;UER00324 |
Reactomei | R-MMU-189451, Heme biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 19044, 4 hits in 17 CRISPR screens |
ChiTaRSi | Ppox, mouse |
PROi | PR:P51175 |
RNActi | P51175, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000062729, Expressed in fetal liver hematopoietic progenitor cell and 295 other tissues |
Genevisiblei | P51175, MM |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR002937, Amino_oxidase IPR036188, FAD/NAD-bd_sf IPR004572, Protoporphyrinogen_oxidase |
Pfami | View protein in Pfam PF01593, Amino_oxidase, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
TIGRFAMsi | TIGR00562, proto_IX_ox, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PPOX_MOUSE | |
Accessioni | P51175Primary (citable) accession number: P51175 Secondary accession number(s): P97344, Q99M34 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families