Ppox

Functionⁱ

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication

Catalytic activityⁱ

Protoporphyrinogen-IX + 3 O₂ = protoporphyrin-IX + 3 H₂O₂.1 Publication

Cofactorⁱ

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Activity regulationⁱ

Inhibited by acifluorfen.1 Publication

Pathwayⁱ: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.
Proteins known to be involved in this subpathway in this organism are:

Protoporphyrinogen oxidase (Ppox), Protoporphyrinogen oxidase (Ppox), Protoporphyrinogen oxidase (Ppox)

This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	42	FAD; via amide nitrogenBy similarity	1
Binding siteⁱ	257	FAD; via amide nitrogen and carbonyl oxygenBy similarity	1
Binding siteⁱ	449	FAD; via amide nitrogenBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	9 – 14	FADBy similarity	6
Nucleotide bindingⁱ	34 – 35	FADBy similarity	2
Nucleotide bindingⁱ	57 – 60	FADBy similarity	4
Nucleotide bindingⁱ	454 – 456	FADBy similarity	3

GO - Molecular functionⁱ

oxygen-dependent protoporphyrinogen oxidase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 3346226

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

heme biosynthetic process
Source: MGI
Inferred from direct assayⁱ
- 3346226
oxidation-reduction process Source: UniProtKB
porphyrin-containing compound biosynthetic process Source: UniProtKB
protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
protoporphyrinogen IX metabolic process Source: MGI
response to drug Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Oxidoreductase
Biological process	Heme biosynthesis, Porphyrin biosynthesis
Ligand	FAD, Flavoprotein

Enzyme and pathway databases

Reactomeⁱ	R-MMU-189451 Heme biosynthesis
UniPathwayⁱ	UPA00251;UER00324

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Protoporphyrinogen oxidase (EC:1.3.3.4) Short name: PPO
Gene namesⁱ	Name:Ppox
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 1

Organism-specific databases

MGIⁱ	MGI:104968 Ppox

Keywords - Cellular componentⁱ

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000135272	1 – 477	Protoporphyrinogen oxidaseAdd BLAST		477

Proteomic databases

EPDⁱ	P51175
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P51175
PaxDbⁱ	P51175
PeptideAtlas More...PeptideAtlasⁱ	P51175
PRIDEⁱ	P51175

PTM databases

iPTMnetⁱ	P51175
PhosphoSitePlusⁱ	P51175

Expressionⁱ

Inductionⁱ

During erythroid differentiation.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000062729 Expressed in 280 organ(s), highest expression level in fetal liver hematopoietic progenitor cell
CleanExⁱ	MM_PPOX
ExpressionAtlasⁱ	P51175 baseline and differential
Genevisibleⁱ	P51175 MM

Interactionⁱ

Subunit structureⁱ

Monomer. Homodimer (By similarity).By similarity

Protein-protein interaction databases

IntActⁱ	P51175, 4 interactors
STRINGⁱ	10090.ENSMUSP00000072863

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P51175
SMRⁱ	P51175
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the protoporphyrinogen oxidase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG1276 Eukaryota COG1232 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000008744
HOGENOMⁱ	HOG000269479
HOVERGENⁱ	HBG001709
InParanoidⁱ	P51175
KEGG Orthology (KO) More...KOⁱ	K00231
OMAⁱ	PMICGIC
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G08PW
PhylomeDBⁱ	P51175
TreeFamⁱ	TF323479

Family and domain databases

Gene3Dⁱ	3.50.50.60, 1 hit
InterProⁱ	View protein in InterPro IPR002937 Amino_oxidase IPR036188 FAD/NAD-bd_sf IPR004572 Protoporphyrinogen_oxidase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01593 Amino_oxidase, 1 hit
SUPFAMⁱ	SSF51905 SSF51905, 1 hit
TIGRFAMsⁱ	TIGR00562 proto_IX_ox, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

P51175-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MGRTVIVLGG GISGLAASYH LIRGPSPPKV ILVEGSKRLG GWIRSIRGSD 
        60         70         80         90        100
GAIFELGPRG IRPAGALGAR TLLLVSELGL ESEVLPVRGD HPAAQNRFLY 
       110        120        130        140        150
VGGTLHPLPS GLRGLLRPSP PFSKPLFWAG LRELLKPRGK EPDETVHSFA 
       160        170        180        190        200
QRRLGPEVAS LAMDSLCRGV FAGNSRELSI RSCFPSLFQA EQTHRSILLG 
       210        220        230        240        250
LLLGAGQSPQ PDSSLIRQAR AERWSQWSLR GGLEVLPQAL HNHLASKGVT 
       260        270        280        290        300
VLSGQPVCGL SLQPEGRWKV SLGDSSLEAD HIISAIPASE LSKLLPAEAA 
       310        320        330        340        350
PLARILSTIK AVSVAVVNLQ YRGACLPVQG FGHLVPSSED PTVLGIVYDS 
       360        370        380        390        400
VAFPEQDGNP PSLRVTVMLG GYWLQKLKAA GHQLSPELFQ QQAQEAAATQ 
       410        420        430        440        450
LGLKEPPSHC LVHLHKNCIP QYTIGHWQKL DSAMQFLTAQ RLPLTLAGAS 
       460        470 
YEGVAVNDCI ESGRQAAVAV LGTESNS

Length:477

Mass (Da):50,871

Last modified:October 1, 1996 - v1

Checksum:ⁱ8CFB48120728DE6F

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A0A6YX50	A0A0A6YX50_MOUSE		Protoporphyrinogen oxidase Protoporphyrinogen oxidase, EC 1.3.3.4	Ppox	389	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	64	A → T (PubMed:7607249).Curated	1
Sequence conflictⁱ	66	A → P (PubMed:7607249).Curated	1
Sequence conflictⁱ	108	L → S in BAA08126 (PubMed:7607249).Curated	1
Sequence conflictⁱ	176	R → Q in AAH02047 (PubMed:15489334).Curated	1
Sequence conflictⁱ	427	W → C in BAA08126 (PubMed:7607249).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U25114 mRNA Translation: AAA96003.1 D45185 mRNA Translation: BAA08126.1 BC002047 mRNA Translation: AAH02047.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS15487.1
PIRⁱ	S65684 S68367
NCBI Reference Sequences More...RefSeqⁱ	NP_032937.1, NM_008911.2 XP_006496770.1, XM_006496707.3
UniGeneⁱ	Mm.266888

Genome annotation databases

Ensemblⁱ	ENSMUST00000073120; ENSMUSP00000072863; ENSMUSG00000062729
GeneIDⁱ	19044
KEGGⁱ	mmu:19044
UCSC genome browser More...UCSCⁱ	uc007dns.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P51175	Protoporhyrinogen oxidase (Fragment)		MOUSE		49	UniRef100_P51175
UPI0005ABB401		MOUSE		315

Protein	Similar proteins		Species	Score	Length	Source
P51175	Protoporhyrinogen oxidase (Fragment)		MOUSE		49	UniRef90_P51175
Protoporphyrinogen oxidase		MESAU		477
Protoporphyrinogen oxidase		RAT		477
Protoporphyrinogen oxidase		DIPOR		477
Protoporphyrinogen oxidase		CRIGR		477
+12

Protein	Similar proteins		Species	Score	Length	Source
P51175	Protoporphyrinogen oxidase	)	HUMAN		477	UniRef50_P50336
Protoporphyrinogen oxidase		BOVIN		477
Protoporphyrinogen oxidase		MACFA		477
Protoporphyrinogen oxidase		PANTR		477
Protoporphyrinogen oxidase		HETGA		505
+238

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U25114 mRNA Translation: AAA96003.1 D45185 mRNA Translation: BAA08126.1 BC002047 mRNA Translation: AAH02047.1
CCDSⁱ	CCDS15487.1
PIRⁱ	S65684 S68367
RefSeqⁱ	NP_032937.1, NM_008911.2 XP_006496770.1, XM_006496707.3
UniGeneⁱ	Mm.266888

3D structure databases

ProteinModelPortalⁱ	P51175
SMRⁱ	P51175
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	P51175, 4 interactors
STRINGⁱ	10090.ENSMUSP00000072863

PTM databases

iPTMnetⁱ	P51175
PhosphoSitePlusⁱ	P51175

Proteomic databases

EPDⁱ	P51175
MaxQBⁱ	P51175
PaxDbⁱ	P51175
PeptideAtlasⁱ	P51175
PRIDEⁱ	P51175

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000073120; ENSMUSP00000072863; ENSMUSG00000062729
GeneIDⁱ	19044
KEGGⁱ	mmu:19044
UCSCⁱ	uc007dns.1 mouse

Organism-specific databases

CTDⁱ	5498
MGIⁱ	MGI:104968 Ppox

Phylogenomic databases

eggNOGⁱ	KOG1276 Eukaryota COG1232 LUCA
GeneTreeⁱ	ENSGT00390000008744
HOGENOMⁱ	HOG000269479
HOVERGENⁱ	HBG001709
InParanoidⁱ	P51175
KOⁱ	K00231
OMAⁱ	PMICGIC
OrthoDBⁱ	EOG091G08PW
PhylomeDBⁱ	P51175
TreeFamⁱ	TF323479

Enzyme and pathway databases

UniPathwayⁱ	UPA00251;UER00324
Reactomeⁱ	R-MMU-189451 Heme biosynthesis

Miscellaneous databases

ChiTaRSⁱ	Ppox mouse
Protein Ontology More...PROⁱ	PR:P51175
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000062729 Expressed in 280 organ(s), highest expression level in fetal liver hematopoietic progenitor cell
CleanExⁱ	MM_PPOX
ExpressionAtlasⁱ	P51175 baseline and differential
Genevisibleⁱ	P51175 MM

Family and domain databases

Gene3Dⁱ	3.50.50.60, 1 hit
InterProⁱ	View protein in InterPro IPR002937 Amino_oxidase IPR036188 FAD/NAD-bd_sf IPR004572 Protoporphyrinogen_oxidase
Pfamⁱ	View protein in Pfam PF01593 Amino_oxidase, 1 hit
SUPFAMⁱ	SSF51905 SSF51905, 1 hit
TIGRFAMsⁱ	TIGR00562 proto_IX_ox, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PPOX_MOUSE
Accessionⁱ	P51175Primary (citable) accession number: P51175 Secondary accession number(s): P97344, Q99M34
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
	Last sequence update:	October 1, 1996
	Last modified:	November 7, 2018
	This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PATHWAY comments
Index of metabolic and biosynthesis pathways

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UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P51175 (PPOX_MOUSE)

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Protoporphyrinogen oxidase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protoporphyrin-IX biosynthesis

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Mitochondrion

Mitochondrion

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Pathwayⁱ: protoporphyrin-IX biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ