UniProtKB - P51164 (ATP4B_HUMAN)
Potassium-transporting ATPase subunit beta
ATP4B
Functioni
The beta subunit of the gastric H+/K+ ATPase pump which transports H+ ions in exchange for K+ ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump (By similarity).
Within a transport cycle, the transfer of a H+ ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle (By similarity).
By similarityGO - Molecular functioni
- ATPase activator activity Source: GO_Central
- heterocyclic compound binding Source: Ensembl
- P-type potassium:proton transporter activity Source: ProtInc
GO - Biological processi
- cell adhesion Source: UniProtKB-KW
- cellular potassium ion homeostasis Source: GO_Central
- cellular sodium ion homeostasis Source: GO_Central
- pH reduction Source: Ensembl
- potassium ion import across plasma membrane Source: GO_Central
- response to lipopolysaccharide Source: Ensembl
- response to organonitrogen compound Source: Ensembl
- sodium ion export across plasma membrane Source: GO_Central
Keywordsi
Biological process | Cell adhesion, Hydrogen ion transport, Ion transport, Potassium transport, Transport |
Ligand | Potassium |
Enzyme and pathway databases
PathwayCommonsi | P51164 |
Reactomei | R-HSA-936837, Ion transport by P-type ATPases |
SignaLinki | P51164 |
Protein family/group databases
TCDBi | 3.A.3.1.2, the p-type atpase (p-atpase) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Potassium-transporting ATPase subunit betaAlternative name(s): Gastric H(+)/K(+) ATPase subunit beta Proton pump beta chain |
Gene namesi | Name:ATP4BImported |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:820, ATP4B |
MIMi | 137217, gene |
neXtProti | NX_P51164 |
VEuPathDBi | HostDB:ENSG00000186009 |
Subcellular locationi
Plasma membrane
- Apical cell membrane By similarity; Single-pass type II membrane protein Sequence analysis
- Cell membrane By similarity; Single-pass type II membrane protein Sequence analysis
Note: Localized in the apical canalicular membrane of parietal cells.By similarity
Plasma Membrane
- apical plasma membrane Source: UniProtKB-SubCell
- plasma membrane Source: Reactome
- sodium:potassium-exchanging ATPase complex Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 36 | CytoplasmicSequence analysisAdd BLAST | 36 | |
Transmembranei | 37 – 57 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 58 – 291 | ExtracellularSequence analysisAdd BLAST | 234 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 496 |
OpenTargetsi | ENSG00000186009 |
PharmGKBi | PA25114 |
Miscellaneous databases
Pharosi | P51164, Tclin |
Chemistry databases
ChEMBLi | CHEMBL2095173 |
DrugBanki | DB05351, Dexlansoprazole DB13620, Potassium gluconate |
DrugCentrali | P51164 |
Genetic variation databases
BioMutai | ATP4B |
DMDMi | 1703461 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219091 | 1 – 291 | Potassium-transporting ATPase subunit betaAdd BLAST | 291 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 99 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Glycosylationi | 103 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Glycosylationi | 130 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Disulfide bondi | 131 ↔ 152 | By similarity | ||
Glycosylationi | 146 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Glycosylationi | 161 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Disulfide bondi | 162 ↔ 178 | By similarity | ||
Glycosylationi | 193 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Disulfide bondi | 201 ↔ 263 | By similarity | ||
Glycosylationi | 222 | N-linked (GlcNAc...) asparagineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
jPOSTi | P51164 |
MassIVEi | P51164 |
PaxDbi | P51164 |
PeptideAtlasi | P51164 |
PRIDEi | P51164 |
ProteomicsDBi | 56294 |
PTM databases
GlyGeni | P51164, 7 sites |
iPTMneti | P51164 |
PhosphoSitePlusi | P51164 |
Expressioni
Gene expression databases
Bgeei | ENSG00000186009, Expressed in body of stomach and 90 other tissues |
Genevisiblei | P51164, HS |
Organism-specific databases
HPAi | ENSG00000186009, Tissue enriched (stomach) |
Interactioni
Subunit structurei
The ATPase pump is composed of two subunits: alpha (catalytic) and beta (regulatory).
Interacts with alpha subunit ATP12A; this interaction is required for the formation of a functionally active pump and targeting at the plasma membrane (By similarity).
Interacts (via N-terminus) with alpha subunit ATP4A (via the P-domain) (By similarity).
By similarityBinary interactionsi
P51164
With | #Exp. | IntAct |
---|---|---|
CCNDBP1 [O95273] | 3 | EBI-3904463,EBI-748961 |
GPR42 [O15529] | 3 | EBI-3904463,EBI-18076404 |
ZC4H2 [Q9NQZ6] | 3 | EBI-3904463,EBI-747993 |
Protein-protein interaction databases
BioGRIDi | 106986, 7 interactors |
ComplexPortali | CPX-2160, Hydrogen:potassium-exchanging ATPase complex |
CORUMi | P51164 |
IntActi | P51164, 6 interactors |
STRINGi | 9606.ENSP00000334216 |
Chemistry databases
BindingDBi | P51164 |
Miscellaneous databases
RNActi | P51164, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 194 – 291 | immunoglobulin-likeAdd BLAST | 98 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3927, Eukaryota |
GeneTreei | ENSGT01030000234579 |
HOGENOMi | CLU_057702_1_1_1 |
InParanoidi | P51164 |
OMAi | CKILADH |
PhylomeDBi | P51164 |
TreeFami | TF314618 |
Family and domain databases
Gene3Di | 2.60.40.1660, 1 hit |
InterProi | View protein in InterPro IPR000402, Na/K_ATPase_sub_beta IPR038702, Na/K_ATPase_sub_beta_sf |
PANTHERi | PTHR11523, PTHR11523, 1 hit |
Pfami | View protein in Pfam PF00287, Na_K-ATPase, 1 hit |
TIGRFAMsi | TIGR01107, Na_K_ATPase_bet, 1 hit |
PROSITEi | View protein in PROSITE PS00390, ATPASE_NA_K_BETA_1, 1 hit PS00391, ATPASE_NA_K_BETA_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAALQEKKTC GQRMEEFQRY CWNPDTGQML GRTLSRWVWI SLYYVAFYVV
60 70 80 90 100
MTGLFALCLY VLMQTVDPYT PDYQDQLRSP GVTLRPDVYG EKGLEIVYNV
110 120 130 140 150
SDNRTWADLT QTLHAFLAGY SPAAQEDSIN CTSEQYFFQE SFRAPNHTKF
160 170 180 190 200
SCKFTADMLQ NCSGLADPNF GFEEGKPCFI IKMNRIVKFL PSNGSAPRVD
210 220 230 240 250
CAFLDQPREL GQPLQVKYYP PNGTFSLHYF PYYGKKAQPH YSNPLVAAKL
260 270 280 290
LNIPRNAEVA IVCKVMAEHV TFNNPHDPYE GKVEFKLKIE K
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M75110 mRNA Translation: AAA35987.1 BX537316 Genomic DNA No translation available. CH471085 Genomic DNA Translation: EAX09221.1 BC029059 mRNA Translation: AAH29059.1 AB008783 Genomic DNA Translation: BAA23425.1 |
CCDSi | CCDS9539.1 |
PIRi | JH0480 |
RefSeqi | NP_000696.1, NM_000705.3 |
Genome annotation databases
Ensembli | ENST00000335288; ENSP00000334216; ENSG00000186009 ENST00000672885; ENSP00000500492; ENSG00000288359 |
GeneIDi | 496 |
KEGGi | hsa:496 |
MANE-Selecti | ENST00000335288.5; ENSP00000334216.3; NM_000705.4; NP_000696.1 |
UCSCi | uc001vtz.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M75110 mRNA Translation: AAA35987.1 BX537316 Genomic DNA No translation available. CH471085 Genomic DNA Translation: EAX09221.1 BC029059 mRNA Translation: AAH29059.1 AB008783 Genomic DNA Translation: BAA23425.1 |
CCDSi | CCDS9539.1 |
PIRi | JH0480 |
RefSeqi | NP_000696.1, NM_000705.3 |
3D structure databases
SMRi | P51164 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 106986, 7 interactors |
ComplexPortali | CPX-2160, Hydrogen:potassium-exchanging ATPase complex |
CORUMi | P51164 |
IntActi | P51164, 6 interactors |
STRINGi | 9606.ENSP00000334216 |
Chemistry databases
BindingDBi | P51164 |
ChEMBLi | CHEMBL2095173 |
DrugBanki | DB05351, Dexlansoprazole DB13620, Potassium gluconate |
DrugCentrali | P51164 |
Protein family/group databases
TCDBi | 3.A.3.1.2, the p-type atpase (p-atpase) superfamily |
PTM databases
GlyGeni | P51164, 7 sites |
iPTMneti | P51164 |
PhosphoSitePlusi | P51164 |
Genetic variation databases
BioMutai | ATP4B |
DMDMi | 1703461 |
Proteomic databases
jPOSTi | P51164 |
MassIVEi | P51164 |
PaxDbi | P51164 |
PeptideAtlasi | P51164 |
PRIDEi | P51164 |
ProteomicsDBi | 56294 |
Protocols and materials databases
Antibodypediai | 25980, 271 antibodies from 30 providers |
DNASUi | 496 |
Genome annotation databases
Ensembli | ENST00000335288; ENSP00000334216; ENSG00000186009 ENST00000672885; ENSP00000500492; ENSG00000288359 |
GeneIDi | 496 |
KEGGi | hsa:496 |
MANE-Selecti | ENST00000335288.5; ENSP00000334216.3; NM_000705.4; NP_000696.1 |
UCSCi | uc001vtz.5, human |
Organism-specific databases
CTDi | 496 |
DisGeNETi | 496 |
GeneCardsi | ATP4B |
HGNCi | HGNC:820, ATP4B |
HPAi | ENSG00000186009, Tissue enriched (stomach) |
MIMi | 137217, gene |
neXtProti | NX_P51164 |
OpenTargetsi | ENSG00000186009 |
PharmGKBi | PA25114 |
VEuPathDBi | HostDB:ENSG00000186009 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3927, Eukaryota |
GeneTreei | ENSGT01030000234579 |
HOGENOMi | CLU_057702_1_1_1 |
InParanoidi | P51164 |
OMAi | CKILADH |
PhylomeDBi | P51164 |
TreeFami | TF314618 |
Enzyme and pathway databases
PathwayCommonsi | P51164 |
Reactomei | R-HSA-936837, Ion transport by P-type ATPases |
SignaLinki | P51164 |
Miscellaneous databases
BioGRID-ORCSi | 496, 3 hits in 1029 CRISPR screens |
GenomeRNAii | 496 |
Pharosi | P51164, Tclin |
PROi | PR:P51164 |
RNActi | P51164, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000186009, Expressed in body of stomach and 90 other tissues |
Genevisiblei | P51164, HS |
Family and domain databases
Gene3Di | 2.60.40.1660, 1 hit |
InterProi | View protein in InterPro IPR000402, Na/K_ATPase_sub_beta IPR038702, Na/K_ATPase_sub_beta_sf |
PANTHERi | PTHR11523, PTHR11523, 1 hit |
Pfami | View protein in Pfam PF00287, Na_K-ATPase, 1 hit |
TIGRFAMsi | TIGR01107, Na_K_ATPase_bet, 1 hit |
PROSITEi | View protein in PROSITE PS00390, ATPASE_NA_K_BETA_1, 1 hit PS00391, ATPASE_NA_K_BETA_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ATP4B_HUMAN | |
Accessioni | P51164Primary (citable) accession number: P51164 Secondary accession number(s): B1B0N8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 165 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 13
Human chromosome 13: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families